Department Infectious Disease Epidemiology

Overview

The focus of the Department Infectious Disease Epidemiology is the performance of studies on communicable diseases of humans. The concept includes the planning, preparation, supervision, performance and analysis of observational studies and clinical trials, combining classical, molecular and clinical epidemiology. Focus diseases are malaria, salmonellosis, cryptosporidiosis, sepsis, neglected tropical diseases (NTD) and co-infections in resource-poor settings. The group is interested in the development, evaluation and application of advanced diagnostics of infectious diseases, integration of electronic surveillance, definition of health priorities, and the analysis of the pathogens and their transmission conditions. The latter includes the analysis of genetic, environmental, socio-economic, demographic, and spatio-temporal risk factors on the host site as well as influence factors of the pathogens and vectors. The superior aim is the translation of results to implementation of diagnostics, treatment, prevention and capacity building in endemic countries. The studies are mainly performed together with the Kumasi Center for Collaborative Research (KCCR), the Ghana School of Public Health of the University of Ghana and different hospitals and communities in the Ashanti Region. Other cooperation partners are in many other African countries as well as in Vietnam and Laos.

Current Research Projects

Fever Without Source (FWS)

According to the WHO treatment guidelines, malaria is defined as fever combined with Plasmodium falciparum parasitaemia. However, there is evidence to question this classification, because of the high frequency of co-infections and asymptomatic parasitaemia in malaria-endemic areas. Due to the lack of appropriate diagnostics it is hardly possible to fully identify possible pathogens causing febrile illnesses beyond malaria in African patients. Thus, the clinical relevance of other infection and co-infections for childhood fever is likely to be underestimated.

While there is increasing evidence that bacterial blood stream infections are an important cause of fever in Africa, information on other pathogens is lacking. Especially, viral bloodstream infections, respiratory pathogens, urinary tract infections and gastrointestinal infections as causes of fever have hardly been studied systematically.

To fully identify pathogens that cause severe febrile illness in children we are conducting a study at Agogo Presbyterian Hospital in rural Ghana. Using a screening-algorithm based on the WHO Integrated Management of Childhood Illness we combine epidemiological field research, clinical research, basic diagnostic techniques and state of the art molecular diagnostics. The study will generate information on the frequency and interactions of specific pathogens and assess associated determinants, such as seasonal and age-related differences. Additionally, clinical symptoms and the clinical course of infections are assessed to identify typical signs for distinct pathogens to establish a diagnostic algorithm for medical personnel.

Contact persons: Benedikt Hogan, Lisa Reigl
Funding: German Center for Infection Research (DZIF)
Cooperation: Dr. Ellis Owusu-Dabo, Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi, Ghana
Yaw Adu-Sarkodie, School of Medical Sciences, Kwame Nkrumah University of Science & Technology (SMS/KNUST), Kumasi, Ghana
Dr. Harry O. Boateng, Children's Ward, Agogo Presbyterian Hospital, Agogo, Asante Akyem, Ghana
Dr. Theresa Rettich, Children's Ward, Agogo Presbyterian Hospital, Agogo, Asante Akyem, Ghana
Dr. Jonas Schmidt-Chanasit, Working Group Arbovirology, BNITM, Hamburg, Germany

Genetic determinants for the transmission of Cryptosporidium parvum/hominis among humans and animals in Africa (C16)

Cryptosporidiosis is increasingly identified as an important cause of morbidity and mortality worldwide. The apicomplexan parasite Cryptosporidium is the causative agent of cryptosporidiosis, recognized as an opportunistic infection in AIDS, but is also responsible for large outbreaks in immunocompetent individuals in developed countries. In developing countries, cryptosporidiosis is a common cause of prolonged and persistent diarrhoea, malnutrition and growth deficits among immunocompetent children and of severe diarrhoea in immunocompromised persons. Cryptosporidium is transmitted anthroponotically or zoonotically depending on the subspecies. Data on the main transmission routes, environmental contamination and animal reservoirs are still missing from sub-Saharan Africa.

The aim of the study is to understand routes of transmission and to identify genetic determinants in the parasite’s genome being responsible for host specificity.

The study is funded by the German Research Foundation (DFG) and will be carried out in Ghana, Gabon, Madagascar and Tanzania.

Contact persons: Daniel Eibach
Funding: German Research Foundation (DFG)
Cooperation: Dr. John Lusingu, National Institute for Medical Research at Tanga Centre, Tanga (NMRI), Tanzania
Dr. Ayola Akim Adégnika, Centre de Recherche Médicale de Lambaréné (CERMEL), Lambaréné, Gabun
Prof. Dr. Raphael Rakotozandrindrainy, Université d' Antananarivo, Laboratoire de Microbiologie et de Parasitologie, Antananarivo, Madagaskar
Dr. Ellis Owusu-Dabo, Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi, Ghana
Prof. Dr. Franz J. Conraths, Institut für Epidemiologie, Friedrich-Loeffler-Institut, Bundesforschungsinstitut für Tiergesundheit

Developing Excellence in Leadership and Genetics Training for Malaria Elimination in sub-Saharan Africa (DELGEME)

DELGEME aims to enrich the pool of African Scientist working in African institutions with relevant expertise particularly for the exploitation of genetics and genomics data for malaria elimination in sub-Saharan Africa.
Over the next five years, DELGEME will be providing short-term and degree training (MSc, PhD, Post-doctoral) to at least 85 young African scientists. Fellowships will be hosted at an African institution with a curriculum in genetics, genomics, bioinformatics, biostatistics or data science. DELGEME fellows are expected to develop a research proposal directed at elucidating critical gaps in malaria genetics and molecular epidemiological data exploration that can aid malaria elimination. Dedicated computational facilities will be available for fellows at The USTTB, the MRC, and USAMRD/KEMRI.
Calls for applications on this exciting exercise is available on the website delgeme.org.

Contact person: Oumou Maiga Ascofaré
Funding: Wellcome Trust Developing Excellence in Leadership, Training and Science Africa Initiative (DELTAS Africa) initiative in partnership with the Department of International Development (DFID) and the Alliance for Accelerating Excellence in Science in Africa (AESA)
Cooperation: University of Science Techniques and Technologies of Bamako Mali (USTTB), Medical Research Council (MRC) Unit The Gambia, The United States Army Medical Research Directorate (USAMRD-K)/KEMRI Kenya, The Noguchi Memorial Institute for Medical Research (NMIMR)-Navrongo Health Research Centre, Ghana, The National Institute of Medical Research (NIMR) Tanzania, Benhard-Nocht Institute for Tropical Medicine (BNITM) Germany/Kumasi Centre for Collaborative Research (KCCR) Ghana, Oxford University - Wellcome Trust Sanger Institute UK, MalariaGEN (www.malariagen.net), Plasmodium Diversity Network Africa (PDNA, www.cggh.org/collaborations/plasmodium-diversity-network-africa)

Assessing the effect of neglected tropical diseases on Plasmodium falciparum transmission in an area of co-endemicity (TransMal)

Schistosoma species, as well as intestinal helminths cause Neglected Tropical Diseases (NTD) with an enormous impact on affected populations. They are highly prevalent in Africa where they share the same geographical distribution with Plasmodium falciparum and consequently affect the same population. Epidemiological and experimental evidence suggests that chronic helminthiasis can alter the host response to a concomitant Plasmodium infection. The influence of helminth on malariometric indices is thought to reflect a profound effect of helminth infection on the host's immune response to P. falciparum infection. Chronic helminth infection has been shown to be associated with an impairment of the protective immune response to Plasmodium.

This latter effect of helminth on malaria transmission is of particular interest and has not yet been fully investigated despite a reported increase in P. falciparum gametocyte carriage in malaria and helminth coinfected subjects by comparison to those without helminths. Therefore, helminths can significantly affect malaria transmission in area of co-endemicity. This effect can interfere with current effort to eliminate malaria in these areas. So far it is unknown how coinfection may affect transmission intensity and there are no published studies that have assessed this question under natural settings.

The proposed study will generate relevant results that will ultimately guide intervention programmes and will provide a basis for improving diagnosis and treatment of infectious diseases in co-endemic countries.

The work will be carried out in collaboration between Ghana (KCCR), Gabon (CERMEL) and Germany (BNITM and University of Tubingen).

Global Partnership Initiated Academia for the Control of Health Threats (GIBACHT)

Capacity building

GIBACHT is an educational programme established and supported by the German Federal Ministry of Foreign Affairs. The training programme focuses on threats and risks conferred by biological agents and bioweapons and their successful prevention and control. The programme is offered and responsibly conducted by the Robert Koch Institute, Berlin, Germany, the Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany, the Swiss Tropical and Public Health Institute, Basel, Switzerland and the African Field Epidemiology Network, AFENET.

GIBACHT Programme Information

Web: www.gibacht.org
Contact: Norbert Schwarz
Cooperations: African Field Epidemiology Network, AFENET
Schweizer Tropen und Public Health Institut, Swiss TPH
Robert Koch Institut, RKI
Funding: German Federal Office of Foreign Affairs

The picture shows the whole GIBACHT team with students from Makerere University in Kampala after having worked through the GIBACHT biosecurity/biosafety case studies
GIBACHT team with students from Makerere University in Kampala

Wound Infection Study

Long persisting wound infections cause morbidity and suffering and require long-term treatment. Treatment success is under serious threat due to the rise of antimicrobial resistance. The bacterial spectrum of microorganisms causing such infections and their antibiotic resistance patterns is only superficially understood, especially in developing coun¬tries that lack adequate surveillance systems and diagnostic facilities. This has hampered specific treatment, which especially applies for chronic wounds, for which standard treatment strategies are ineffective.
This study takes place at Agogo Presbyterian Hospital in rural Ghana and aims to

  • Study the microbial composition of infected chronic wounds in adults presenting to the outpatient department
  • Study antimicrobial resistance and drug resistance mechanisms of the isolated bacterial strains

Contact persons: Denise Dekker, Benedikt Hogan

Prevalence of selected bacteria in chicken meat in Kumasi (Poultry study)

Frequent overuse of antibiotics in poultry farming has contributed to the emergence of antibiotic resistance. Consumption of meat contaminated with Salmonella, Campylobacter, and Arcobacter may infect humans and lead to emergence of multidrug resistance. Although the general transmission mode for the enteric bacteria under investigation is known (faecal-oral), it remains speculative which pathogen vehicles are predominant in Africa.In Ghana, chicken meat is locally available, however the majority is imported from Brazil, the United States, or the Netherlands.
This study takes place in Kumasi in urban Ghana and aims to

  • Investigate the occurrence and molecular types of multidrug resistant Salmonella spp., Campylobacter spp. Arcobacter spp. and ESBL- producing E. coli in local and imported chicken meat
  • Detect the presence of resistance genes in Salmonella, Campylobacter, Arcobacter and ESBL E. coli by PCR and sequencing

Contact persons: Denise Dekker, Daniel Eibach
Cooperation: Robert Koch - Institut (RKI), Wernigerode
Institute for Medical Microbiology, Goettingen

Influenza Surveillance Ashanti (ISA)

Epidemiology of Influenza A viruses circulating in animals and animal-handlers in Ghana

Influenza A viruses affect a wide range of hosts including poultry, pigs and humans. Usually, transmission between birds and mammals is restricted by host adaptation - avian viruses do not replicate well in mammalian hosts and vice versa. Influenza viruses have evolved mechanisms to overcome host restrictions and infect other species, for example through reassortment of gene segments during simultaneous infection of two virus subtypes. Poultry and pigs are a major source of protein and employment for the population of Ghana. These agricultural commodities contribute substantially to the food security, which is threatened by recent outbreaks of highly pathogenic avian H5N1. Although no human infection has yet been recorded, animal-handlers remain at high risk from infection by H5N1 and other Influenza A subtypes circulating in domestic animals. Data on the epidemiology of Influenza A viruses in the animal sector in Ghana is scarce. Our study aims to determine the prevalence and subtypes of circulating Influenza A viruses in poultry, pigs and their respective animal-handlers in Ghana. We further aim to assess the risk that these isolates pose to human hosts using phenotypic markers of increased transmissibility and pathogenicity.

Contact person: Eva Mertens, Matilda Ayim-Akonor
Funding: DAAD, Ghana Ministry of Education, West African Agricultural Productivity Program (WAAPP)
Cooperation: Dr. Ellis Owusu-Dabo, Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi, Ghana
Dr. E.K Adu, Council for Scientific and Industrial Research (CSIR)- Animal Research Institute, Accra, Ghana

Past Research Projects

Surveillance of Ebola infections by real-time mobile data transmission in Nigeria (EBOKON)

The ongoing Ebola virus epidemic in West Africa and the possible re-emergence of new Ebola virus infections in countries such as Nigeria require to strengthen the disease surveillance and control capabilities in these countries.
The aim of the project "EBOKON 10" is to exploit information technology to support in identifying emerging infections and Ebola suspected cases as well as their contacts immediately. In this way, further spread of infections can be prevented. The Department of Epidemiology at the HZI (Prof. Dr. Krause) has initiated that project together with the Department Infectious Disease Epidemiology at BNITM, the Robert Koch Institute, the Hasso Plattner Institute and the Nigerian "Programme for Field Epidemiology and Laboratory Training" to address this challenge.
In order to meet the specific technical requirements of West African countries, a management tool for infection control and for real time transmission of epidemiological data will be developed. It will run on normal mobile phones that are widely spread in West African countries. The complexity of the data processing is, however, handled by a high-performance data management platform and the use of cloud technology provided by SAP, a German Software Corporation.

Contact persons: Norbert Schwarz, Maike Lamshöft, Lisa Reigl
Cooperation: Helmholtz Centre for Infection Research, Braunschweig
Nigeria Field Epidemiology & Laboratory Training Program (NFELTP), Abuja, Nigeria
Robert Koch-Institut (RKI), Berlin
Federal Ministry of Health (FMOH), Abuja, Nigeria
Deutsche Zentrum für Infektionsforschung (DZIF), Braunschweig
Hasso-Plattner-Institut, Potsdam

EBOKON(10) Team

Prevalence of Staphylococcous aureus and Methicillin resistant S. aureus (MRSA) in healthy carriers in Madagascar

Staphylococcus aureus (S. aureus) is one of the most commonly isolated pathogen and can cause a variety of illnesses ranging from skin and soft tissue infections to life-threatening disease. It can be found as a commensal bacterium colonizing the anterial nares of asymptomatic carriers who may transmit the pathogen in the community or in health care facilities. Since the introduction of beta-lactam antibiotics, the spread of methicillin resistant S. aureus (MRSA) is rising globally . Recent efforts include the creation of a MRSA network in Africa that covers Gabon, Tanzania and Mocambique. However, data on methicllin sensitive S. aureus (MSSA) and MRSA in Africa and especially Madagascar remains scarce.

To describe the epidemiology of S. aureus and MRSA in Madagascar, we took nasal swabs from healthy students of the University of Antananarivo as well as from health care workers from different hospitals. In cooperation with the Laboratory of Microbiology and Parasitology of the University of Antananarivo, we analyzed the samples with conventional microbiological methods in Madagascar in 2013. Further detailed analyses of the isolates are currently performed in Germany.

The aim of this study is to describe the prevalence of MSSA and MRSA among healthy students and health care workers in Antananarivo, Madagascar. New data might guide the implementation of preventive measures in Madagascan hospitals.

Contact persons: Benedikt Hogan, Norbert Schwarz
Cooperation: Prof. Raphael Rakotozandrindrainy and staff, Laboratory for Microbiology and Parasitology, University of Antananarivo, Madagascar

A mobile-phone based Electronic Health Information and Surveillance System (eHISS) for Africa: concept and pilot study

In resource-poor settings mobile phones can be used to disseminate life-saving health information. Via such electronic health information systems (eHealth) even illiterate individuals or people living in remote areas can obtain valuable information on the prevention or treatment of acute diseases. However, most eHealth approaches are unidirectional and available to health-care workers only. An alternative approach is being conceptualized and developed in this project. Here a mobile phone-based “Electronic Health Information and Surveillance System” (eHISS) is being established and piloted in Ghana. Diseased individuals have the possibility to call a project hotline linked to an Interactive Voice Response (IVR) system. After sequential questions on disease symptoms have been answered, tailored health information is provided. Additionally, the place, time and type of these requests is recorded and processed to display real-time spatio-temporal disease patterns using digital maps. This approach enables automated real-time syndrome surveillance and early identification of potential disease outbreaks. The complex nature of eHISS is represented in the multi-disciplinary project consortium, involving epidemiology, health geography, clinical research, public health and eHealth techniques.

Contact person: Ralf Krumkamp, Maike Lamshöft
Funding: Ministry of Research and Education (BMBF)
Cooperation: Dr. Ellis Owusu-Dabo, Kumasi Centre for Collaborative Research (KCCR), Ghana
Dr Julius Fobil, Ghana School of Public Health (GSPH), Accra / Ghana
Dr. Benno Kreuels, University Clinic Hamburg Eppendorf (UKE), Germany
Prof. Dr. Mirjam Kretzschmar, National Institute for Public Health and the Environment, Bilthoven, The Netherlands
Dr. Mark Boots, VOTOMobile, Kumasi, Ghana

African Programme for Advanced Research Epidemiology Training (APARET)

Capacity Building

APARET was initiated in cooperation with the African Field Epidemiology Network, AFENET (www.afenet.net) and 11 other partners from Africa, Europe and America. APARET is an advanced training initiative whose goal is to support independent research activities by graduates of Field Epidemiology Training Programmes (FETP) and Field Epidemiology Laboratory Training Programmes (FELTP) in Africa. The four-year programme is supported by a grant of 2 million Euros from the European Union through the Seventh Framework Programme for the Funding of research and technological development... (more)

Contact: Norbert Schwarz, Lisa Reigl
Cooperations: Bernhard Nocht Institute for Tropical Medicine, Hamburg (BNITM), Germany
African Field Epidemiology Network (AFENET), Uganda
WHO Multi Disease Surveillance Centre (MDSC) in Ougadougou, Burkina-Faso
Ghana School of Public Health, Accra (GSPH), Ghana
Kwame Nkrumah University of Science and Technology , Centre for Collaborative Research in Tropical Medicine, Kumasi (KNUST), Ghana
Makerere University, School of Public Health, Kampala (MUSPH), Uganda
University of Ouagadougou (UO), Burkina-Faso
University of Zimbabwe (UZ), Zimbabwe
Muhimbili University of Health and Allied Sciences (MUHAS), Tanzania
Ahmadu Bello University (ABU), Zaria, Nigeria
Swiss Tropical and Public Health Institute (Swiss TPH), Switzerland
Centers for Disease Control and Prevention (CDC), Atlanta, USA
Funding: FP7 programme of the European Commission

Typhoid Fever Surveillance in East and West Africa (TySA/FISA/TSAP) Project

Typhoid fever is a systemic infection caused by Salmonella enterica serotype Typhi (S. Typhi). The disease is an important problem in developing countries because of its morbidity and mortality as well as the emergence of multidrug-resistant (MDR) S. Typhi strains. Since typhoid fever is a water- and food-borne gastrointestinal infection, ensuring safe water, adequate sanitation and hygiene are the main strategies to prevent the disease. In the absence of an affordable program to assure safe water and better sanitary conditions in less developed countries, efforts have been directed towards primary prevention through vaccination. Policymakers of several developing countries have indicated that updated data on the incidence of typhoid in their countries would be essential to introduce the vaccines into their programs.

The aim of this study is to begin to assess the burden of typhoid fever in sub-Saharan Africa. With the exception of case descriptions and outbreak reports, there is very little published data available on typhoid fever in sub-Saharan Africa. The specific objective would be to conduct a blood culture- and serology-based typhoid fever surveillance in a hospital in the Ashanti Region in Ghana, to determine the proportion of blood culture-confirmed typhoid fever cases among children and adults presenting to these hospital with fever, the proportion of typhoid fever with non-bacterial co-infections (i.e. malaria), the antimicrobial susceptibility pattern of S. Typhi isolates, the clinical presentation among children and adults, and the patterns of antimicrobial use prior to hospital presentation. The study results shall provide much-needed information on typhoid fever in sub-Saharan Africa and, if successful, provide the basis for potential extension of the surveillance to a larger area in Ghana.

Contact person: Jürgen May, Ralf Krumkamp
Funding: Ministry of Foreign Affairs and Trade, Seoul, Republic of Korea
Cooperation: Florian Marks, International Vaccine Institute (IVI), Seoul, Republic of Korea
Yaw Adu-Sarkodie, School of Medical Sciences, Kwame Nkrumah Univeristy of Science & Technology (SMS/KNUST), Kumasi, Ghana
Frank Huenger, Kumasi Centre for Collaborative Research (KCCR), Kumasi, Ghana
William Nana Arhin Thompson, Agogo Presbyterian Hospital, Ghana

Intermittent Preventive Antimalarial Treatment in Infants (IPTi)

Sulfadoxine-Pyrimethamine-based IPTi is considered a promising measure for malaria control. Still, the value of IPTi and similar malaria control approaches in older children (IPTc) and in areas of seasonal malaria transmission (IPTs) is intensely discussed. It is expected that WHO will soon launch a policy recommendation for a broad implementation of IPTi in Africa on the basis of published studies.
We conducted a controlled and registered trial on IPTi (registered at ClinicalTrial; NCT00206739) from 2003 until September 2005 in the Ashanti Region of Ghana, an area with intense perennial malaria transmission. In addition we evaluated whether an extended IPTi schedule might prolong the protection against malaria. Our research contributes to the ongoing discussion on IPTi and should be considered for the formulation of the WHO policy recommendation for implementation led by the IPTi consortium (http://www.ipti-malaria.org/).

The protection against malaria and anaemia in our trial was considerably lower than in the first IPTi trial published 2001 in The Lancet (Schellenberg et al.). In addition, our data indicate an age-dependence of the treatment efficacy and suggest that the protective effect cannot be extended by an additional drug application beyond the EPI schedule. The main results are published in the Journal of Clinical Infectious Diseases (see article).

To drive our understanding of IPTi forward and we are currently performing analyses on how protective efficacy is relation to factors like drug resistance, malaria incidence and socio-economic and spatio-temporal factors. In addition, we evaluate the impact of IPTi on the development of immunity, the possible influence of the intervention on sub-clinical organ dysfunction due to chronic P. falciparum infection, and more.

Contact person: Jürgen May, Benno Kreuels
Funding: BMBF, DFG, DAAD

Spatio-temporal and socioeconomic risk factors of malaria in children from Ghana

Malaria is the most common vector-borne infectious disease with more than one million deaths each year where Sub-Saharan Africa carries most of the burden. In contrast to a retrogressive trend in some areas morbidity and mortality from malaria have been increasing in many other areas due to factors such as deteriorating health systems, growing drug and insecticide resistance, failure of water management. The potential influence of climate, socioeconomic, sociodemographic and land cover factors on the emergence of malaria is under intensive discussion.
The objectives of this project are to investigate spatio-temporal patterns of malaria in children from Ghana below the age of 15 years. Specifically, we investigated how far malaria is associated with a position of a houshold in the area, the land use around homesteads, precipitation and temperature, and other environmental factors in the context of the socioeconomic and sociodemographic situation.
First results showed that the socioeconomic situation is significantly associated with malaria even in holoendemic rural areas. Additionally, a temporal influence of the level of precipitation on malaria incidence with a time lag of 9 weeks could be demonstrated. By using Geographical Information Systems (GIS) the spatial analysis of remotely sensed data demonstrated an influence of specific land cover classes on malaria incidence.

Funding: Swiss Foundation

A comparative assessment of the effectiveness of Artemether plus Lumefantrine (Coartem®) versus Artesunate plus Amodiaquine (Arsucam®) for the treatment of children with uncomplicated Plasmodium falciparum malaria.

Childhood mortality related to Plasmodium falciparum malaria is on the rise with more than 1 million deaths per year in Sub-Saharan Africa. In the context of growing drug-resistance to antimalarials health officials are calling for rapid replacement of failing drugs by combining antimalarial drugs. Artemisinin combination therapies (ACTs) are in the focus of international and national malaria control programs and are recommended for first-line treatment of malaria by the World Health Organisation (WHO). ACTs have been reported to be highly effective as artemisinin derivatives cause a rapid and substantial decrease in the parasite load when used for treating patients with malaria. However, the short half-lives of these drugs result in frequent recrudescent infections when used alone.

Therefore, much interest lays on the choice of the combination partner drug. Furthermore, drug resistance of parasites to the respective partner drug is a matter of concern. Artesunate-amodiaquine and artemether-lumefantrine are the only two registered fixed-dose ACTs available in African countries, which are GMP-manufactured at industrial scale. There is still limited data from randomised, controlled trials to compare effectiveness of these two ACTs in Africa, including Ghana. More data is needed to support their general effectiveness and to enable policy-makers to make evidence-based first-line treatment decisions. In this study, which is registered at ClinicalTrials under the number NCT00374205, clinical researchers of our group in Hamburg collaborate with scientists of the Kumasi Centre for Collaborative Research (KCCR), the School of Medical Sciences Kumasi (SMS/KNUST) and the Presbyterian Health Service (PHS) in Ghana. The study is co-funded by the “Vereinigung der Freunde des Tropeninstituts Hamburg e.V” and results are expected in fall 2007.

The study was co-funded by the “Vereinigung der Freunde des Tropeninstituts Hamburg e.V” and results have been published in the free online access Malaria Journal (see article).

Team AmoArte

Neglected infections as causes of morbidity and mortality in African children under five years of age - establishment of an advanced diagnostic system

During a period of one year all children admitted to the under-five outpatient clinic of a rural teaching hospital will consecutively recruited for the study. A standardized approach of clinical examination shall be established and performed on the basis of the WHO Integrated Management of Childhood Illness (IMCI) strategy.
The specific causes of infectious diseases, co-infections and co-morbidity will be assessed by advanced molecular diagnosis and the clinical significance will be evaluated. In addition to conventional microbiological techniques (bacterial cultures, serodiagnoses), DNA of infectious agents extracted from materials such as blood, stool, and sputum will be amplified by standard and real-time PCR assays and a panel of different techniques will be applied to determine and further specify causative organisms. This includes the collection of samples for fine-typing of strains, detection of drug-resistance markers and other sub-analyses.

The specific objectives of this proposal are

  1. to establish a standardized IMCI-based diagnostic system for infectious diseases (Core Module);
  2. to develop simple clinical algorithms for the direction of patients to specific investigations and treatment schemes (module 1);
  3. to assess morbidity and mortality of Ghanaian children under five years of age due to invasive bacterial infections and their participation and role in various co-infections, including malaria (module 2);
  4. to determine the significance of distinct pathogens as diarrhea-causing agents (module 3);
  5. to evaluate the specific viral aetiology of acute respiratory infections (module 4).

Funding: Swiss Foundation

Genetic association approach to identify endothelial receptors for plasmodium falciparum

Adherence of parasitized red blood cells (PRBC) to deep-organ vascular endothelium is essential for the pathogenesis of cerebral malaria and other severe and life-threatening malaria complications. In addition, the acquisition of natural immunity to malaria was found to correlate with the recognition of PRBC adherence ligands by serum antibodies.
Despite the obvious medical relevance of the adherence process, the knowledge of host and parasite molecules involved appears incomplete possibly because it is difficult to obtain by cell biology, as circumstantial evidence indicates.

We propose to apply a genetic association approach to identify human endothelial receptors for PRBC by comparing affected patients with persons who appear not susceptible. A total of 25 candidates including the established PRBC adherence receptors and new ones selected on the basis of functional and topographical criteria will be screened for genetic variants, which will be subjected to an association study using 2500 cases of severe malaria, 1500 sex-, age-, and residence-matched controls and 800 parent-affected offspring trios recruited in NGFN-1. Stratification for the type of malaria complication possibly allows conclusions concerning brain-specific functions of receptors. Identifying human endothelial receptors and subsequently PRBC ligands involved may provide a basis for vaccine development in malaria.

Funding: BMBF

Publication Highlights

Arbeitsgruppe May (Infektionsepidemiologie)

Association between malaria and invasive nontyphoidal Salmonella infection in a hospital study: Accounting for Berkson’s bias
Krumkamp R, Kreuels R, Sarpong N, Boahen KG, Foli G, Hogan B, Jaeger A, Reigl L, Zeeb H, Marks F, Adu-Sarkodie Y, May J.
Clin Infect Dis 2016;62:S83-9

The emergence of reduced ciprofloxacin susceptibility in Salmonella enterica causing bloodstream infections in rural Ghana
Eibach D, Al-Emran HM, Dekker D, Krumkamp R, Adu-Sarkodie Y, Cruz Espinoza LM, Ehmen C, Boahen K, Heisig P, Im J, Jaeger A, von Kalckreuth V, Pak GD, Panzner U, Park SE, Reinhardt A, Sarpong N, Schütt-Gerowitt H, Wierzba TF, Marks F, May J.
Clin Infect Dis. 2016 Mar 15;62 Suppl 1:S32-6. PubMed PMID: 26933017

Long-term live imaging reveals cytosolic immune responses of host hepatocytes against Plasmodium infection and parasite escape mechanisms
Prado M, Eickel N, De Niz M, Heitmann A, Agop-Nersesian C, Wacker R, Schmuckli-Maurer J, Caldelari R, Janse CJ, Khan SM, May J, Meyer CG, Heussler VT.
Autophagy. 2015 Sep 2;11(9):1561-79. PubMed PMID: 26208778

The causal effect of malaria on stunting: a Mendelian randomization and matching approach
Kang H, Kreuels B, Adjei O, Krumkamp R, May J, Small DS
Int J Epidemiol. 2013 Oct;42(5):1390-8. PubMed PMID: 23925429

Genome-wide association study indicates two novel resistance loci for severe malaria
Timmann C, Thye T, Vens M, Evans J, May J, Ehmen C, Sievertsen J, Muntau B, Ruge G, Loag W, Ansong D, Antwi S, Asafo-Adjei E, Nguah SB, Kwakye KO, Akoto AO, Sylverken J, Brendel M, Schuldt K, Loley C, Franke A, Meyer CG, Agbenyega T, Ziegler A, Horstmann RD
Nature. 2012 Sep 20;489(7416):443-6. PubMed PMID: 22895189

Differing effects of HbS and HbC traits on uncomplicated falciparum malaria, anemia, and child growth
Kreuels B, Kreuzberg C, Kobbe R, Ayim-Akonor M, Apiah-Thompson P, Thompson B, Ehmen C, Adjei S, Langefeld I, Adjei O, May J
Blood. 2010 Jun 3;115(22):4551-8. PubMed PMID: 20231425

Efficacy and safety of intermittent preventive treatment with sulfadoxine-pyrimethamine for malaria in African infants: a pooled analysis of six randomised, placebo-controlled trials
Aponte JJ, Schellenberg D, Egan A, Breckenridge A, Carneiro I, Critchley J, Danquah I, Dodoo A, Kobbe R, Lell B, May J, Premji Z, Sanz S, Sevene E, Soulaymani-Becheikh R, Winstanley P, Adjei S, Anemana S, Chandramohan D, Issifou S, Mockenhaupt F, Owusu-Agyei S, Greenwood B, Grobusch MP, Kremsner PG, Macete E, Mshinda H, Newman RD, Slutsker L, Tanner M, Alonso P, Menendez C
Lancet. 2009 Oct 31;374(9700):1533-42. PubMed PMID: 19765816

Hemoglobin variants and disease manifestations in severe falciparum malaria
May J, Evans JA, Timmann C, Ehmen C, Busch W, Thye T, Agbenyega T, Horstmann RD.
JAMA. 2007 May 23;297(20):2220-6.

All Publications

2016

Molecular Epidemiology and Antibiotic Susceptibility of Vibrio cholerae associated with a large Cholera Outbreak in Ghana in 2014
Eibach D, Herrera-León S, Gil H, Hogan B, Ehlkes L, Adjabeng M, Kreuels B, Nagel M, Opare D, Fobil JN, May J
PLOS Neglected Tropical Diseases, 2016 May 27;10(5):e0004751. eCollection 2016 May

Extended spectrum beta-lactamase producing Enterobacteriaceae causing bloodstream infections in rural Ghana, 2007-2012
Eibach D, Belmar Campos C, Krumkamp R, Al-Emran HM, Dekker D, Boahen KG, Kreuels B, Adu-Sarkodie Y, Aepfelbacher M, Se Eun Park SE, Panzner U, Marks F, May J
Int J Med Microbiol. 2016 May 10. pii: S1438-4221(16)30061-3. PubMed PMID: 27222489

Association Between Malaria and Invasive Nontyphoidal Salmonella Infection in a Hospital Study: Accounting for Berkson's Bias
Krumkamp R, Kreuels B, Sarpong N, Boahen KG, Foli G, Hogan B, Jaeger A, Reigl L, Zeeb H, Marks F, Adu-Sarkodie Y, May J.
Clin Infect Dis. 2016 Mar 15;62 Suppl 1:S83-9. PMID: 26933027

Variations of Invasive Salmonella Infections by Population Size in Asante Akim North Municipal, Ghana
Cruz Espinoza LM, Nichols C, Adu-Sarkodie Y, Al-Emran HM, Baker S, Clemens JD, Dekker DM, Eibach D, Krumkamp R, Boahen K, Im J, Jaeger A, von Kalckreuth V, Pak GD, Panzner U, Park SE, Park JK, Sarpong N, Schütt-Gerowitt H, Toy T, Wierzba TF, Marks F, May J.
Clin Infect Dis. 2016 Mar 15;62 Suppl 1:S17-22. PMID: 26933015

The Emergence of Reduced Ciprofloxacin Susceptibility in Salmonella enterica Causing Bloodstream Infections in Rural Ghana
Eibach D, Al-Emran HM, Dekker DM, Krumkamp R, Adu-Sarkodie Y, Cruz Espinoza LM, Ehmen C, Boahen K, Heisig P, Im J, Jaeger A, von Kalckreuth V, Pak GD, Panzner U, Park SE, Reinhardt A, Sarpong N, Schütt-Gerowitt H, Wierzba TF, Marks F, May J.
Clin Infect Dis. 2016 Mar 15;62 Suppl 1:S32-6. PMID: 26933017

A Multicountry Molecular Analysis of Salmonella enterica Serovar Typhi With Reduced Susceptibility to Ciprofloxacin in Sub-Saharan Africa
Al-Emran HM, Eibach D, Krumkamp R, Ali M, Baker S, Biggs HM, Bjerregaard-Andersen M, Breiman RF, Clemens JD, Crump JA, Cruz Espinoza LM, Deerin J, Dekker DM, Gassama Sow A, Hertz JT, Im J, Ibrango S, von Kalckreuth V, Kabore LP, Konings F, Løfberg SV, Meyer CG, Mintz ED, Montgomery JM, Olack B, Pak GD, Panzner U, Park SE, Razafindrabe JL, Rabezanahary H, Rakotondrainiarivelo JP, Rakotozandrindrainy R, Raminosoa TM, Schütt-Gerowitt H, Sampo E, Soura AB, Tall A, Warren M, Wierzba TF, May J*, Marks F.
Clin Infect Dis. 2016 Mar 15;62 Suppl 1:S42-6. PMID: 26933020. [*contributed equally]

Analysis of diagnostic findings from the European Mobile Laboratory in Guéckédou, Guinea, March 2014 through March 2015
Kerber R, Krumkamp R, Diallo B, Jaeger A, Rudolf M, Lanini S, Bore JA, Koundouno FR, Becker-Ziaja B, Fleischmann E, Stoecker K, Meschi S, Mély S, Newman ENC, Carletti F, Portmann J, Korva M, Wolff S, Molkenthin P, Kis Z, Kelterbaum A, Bocquin A, Strecker T, Fizet A, Castilletti C, Schudt G, Ottowell L, Kurth A, Atkinson B, Badusche M, Cannas A, Pallasch E, Bosworth A, Yue C, Pályi B, Ellerbrok H, Kohl C, Oestereich L, Logue CH, Lüdtke A, Richter M, Ngabo D, Borremans B, Becker D, Gryseels S, Abdellati S, Vermoesen T, Kuisma E, Kraus A, Liedigk B, Maes P, Thom R, Duraffour S, Diederich S, Hinzmann J, Afrough B, Repits J, Mertens M, Vitoriano I, Bah A, Sachse A, Boettcher JP, Wurr S, Bockholt S, Nitsche A, Strasser M, Ippolito G, Becker S, Raoul H, Carroll MW, De Clerck H, Van Herp M, Sprecher A, Koivogui L, Keïta S, Drury P, Gurry C, Formenty P, May J, Gabriel M, Wölfel R, Günther S, Di Caro A
J Infect Dis. 2016 Oct 15;214(suppl 3):S250-S257

Is the A578S Single-Nucleotide Polymorphism in K13-propeller a Marker of Emerging Resistance to Artemisinin Among Plasmodium falciparum in Africa?
Maïga-Ascofaré O, May J
J Infect Dis. 2016 Jan 1;213(1):165-6. Epub 2015 Aug 12. PubMed PMID: 26268852

Prevalence of nasal colonisation by methicillin-sensitive and methicillin-resistant Staphylococcus aureus among healthcare workers and students in Madagascar
Hogan BJ, Rakotozandrindrainy R, Al-Emran H, Dekker D, Hahn A, Jaeger A, Poppert S, Frickmann H, Hagen RM, Micheel V, Crusius S, Randriamampionona N, Rakotondrainiarivelo JP, Razafindrabe T, May J, Schwarz NG
BMC Infect Dis. 2016; 16: 420. Published online 2016 Aug 15. doi: 10.1186/s12879-016-1733-6 PMCID: PMC4986198

Spatial heterogeneity of malaria in Ghana: a cross-sectional study on the association between urbanicity and the acquisition of immunity
Frank C, Krumkamp R, Sarpong N, Sothmann P, Fobil JN, Foli G, Jaeger A, Ehlkes L, Owusu-Dabo E, Adu-Sarkodie Y, Marks F, Schumann RR, May J, Kreuels B.
Malar J. 2016 Feb 11;15(1):84. PMID: 26867774

Application of a multiplex PCR assay for the detection of gastrointestinal pathogens in a rural African setting
Eibach D, Krumkamp R, Hahn A, Sarpong N, Adu-Sarkodie Y, Leva A, Käsmaier J, Panning M, May J, Tannich E
BMC Infect Dis. 2016 Apr 14;16(1):150. doi: 10.1186/s12879-016-1481-7

Saccharomyces boulardii to Prevent Antibiotic-Associated Diarrhea: A Randomized, Double-Masked, Placebo-Controlled Trial
Ehrhardt S, Guo N, Hinz R, Schoppen S, May J, Reiser M, Schroeder MP, Schmiedel S, Keuchel M, Reisinger EC, Langeheinecke A, de Weerth A, Schuchmann M, Schaberg T, Ligges S, Eveslage M, Hagen RM, Burchard GD, Lohse AW
Open Forum Infect Dis. 2016 Jan 29;3(1):ofw011. PubMed PMID: 26973849

Similar virus spectra and seasonality in paediatric patients with acute respiratory disease, Ghana and Germany
Annan A, Ebach F, Corman VM, Krumkamp R, Adu-Sarkodie Y, Eis-Hübinger AM, Kruppa T, Simon A, May J, Evans J, Panning M, Drosten C, Drexler JF
Clin Microbiol Infect. 2016 Apr;22(4):340-6. PubMed PMID: 26585774

Tropheryma whipplei in children with diarrhoea in rural Ghana
Vinnemeier CD, Klupp EM, Krumkamp R, Rolling T, Fischer N, Owusu-Dabo E, Addo MM, Adu-Sarkodie Y, Käsmaier J, Aepfelbacher M, Cramer JP, May J, Tannich E.
Clin Microbiol Infect. 2016 Jan;22(1):65.e1-3. Epub 2015 Oct 8. PMID: 26456475

Validation and Identification of Invasive Salmonella Serotypes in Sub-Saharan Africa by Multiplex Polymerase Chain Reaction
Al-Emran HM, Krumkamp R, Dekker DM, Eibach D, Aaby P, Adu-Sarkodie Y, Ali M, Rubach MP, Bjerregaard-Andersen M, Crump JA, Cruz Espinoza LM, Løfberg SV, Gassama Sow A, Hertz JT, Im J, Jaeger A, Kabore LP, Konings F, Meyer CG, Niang A, Pak GD, Panzner U, Park SE, Rabezanahary H, Rakotozandrindrainy R, Raminosoa TM, Razafindrabe TJ, Sampo E, Schütt-Gerowitt H, Sarpong N, Soura AB, Tall A, von Kalckreuth V, Wierzba TF, May J, Marks F.
Clin Infect Dis. 2016 Mar 15;62 Suppl 1:S80-2. PMID: 26933026

The Typhoid Fever Surveillance in Africa Program (TSAP): Clinical, Diagnostic, and Epidemiological Methodologies
von Kalckreuth V, Konings F, Aaby P, Adu-Sarkodie Y, Ali M, Aseffa A, Baker S, Breiman RF, Bjerregaard-Andersen M, Clemens JD, Crump JA, Cruz Espinoza LM, Deerin JF, Gasmelseed N, Sow AG, Im J, Keddy KH, Cosmas L, May J, Meyer CG, Mintz ED, Montgomery JM, Olack B, Pak GD, Panzner U, Park SE, Rakotozandrindrainy R, Schütt-Gerowitt H, Soura AB, Warren MR, Wierzba TF, Marks F
Clin Infect Dis. 2016 Mar 15;62 Suppl 1:S9-S16. PMID: 26933028

Prevalence of Salmonella Excretion in Stool: A Community Survey in 2 Sites, Guinea-Bissau and Senegal
Im J, Nichols C, Bjerregaard-Andersen M, Sow AG, Løfberg S, Tall A, Pak GD, Aaby P, Baker S, Clemens JD, Espinoza LM, Konings F, May J, Monteiro M, Niang A, Panzner U, Park SE, Schütt-Gerowitt H, Wierzba TF, Marks F, von Kalckreuth V.
Clin Infect Dis. 2016 Mar 15;62 Suppl 1:S50-5. PMID: 26933022

Detection of a Novel gyrB Mutation Associated With Fluoroquinolone-Nonsusceptible Salmonella enterica serovar Typhimurium Isolated From a Bloodstream Infection in Ghana
Al-Emran HM, Heisig A, Dekker D, Adu-Sarkodie Y, Cruz Espinoza LM, Panzner U, von Kalckreuth V, Marks F, Park SE, Sarpong N, May J, Heisig P.
Clin Infect Dis. 2016 Mar 15;62 Suppl 1:S47-9. PMID: 26933021

The Relationship Between Invasive Nontyphoidal Salmonella Disease, Other Bacterial Bloodstream Infections, and Malaria in Sub-Saharan Africa
Park SE, Pak GD, Aaby P, Adu-Sarkodie Y, Ali M, Aseffa A, Biggs HM, Bjerregaard-Andersen M, Breiman RF, Crump JA, Cruz Espinoza LM, Eltayeb MA, Gasmelseed N, Hertz JT, Im J, Jaeger A, Parfait Kabore L, von Kalckreuth V, Keddy KH, Konings F, Krumkamp R, MacLennan CA, Meyer CG, Montgomery JM, Ahmet Niang A, Nichols C, Olack B, Panzner U, Park JK, Rabezanahary H, Rakotozandrindrainy R, Sampo E, Sarpong N, Schütt-Gerowitt H, Sooka A, Soura AB, Sow AG, Tall A, Teferi M, Yeshitela B, May J, Wierzba TF, Clemens JD, Baker S, Marks F.
Clin Infect Dis. 2016 Mar 15;62 Suppl 1:S23-31. PMID: 26933016

High detection rate of Rickettsia africae in Amblyomma variegatum but low prevalence of anti-rickettsial antibodiesin healthy pregnant women in Madagascar
Keller C, Krüger A, Schwarz NG, Rakotozandrindrainy R, Rakotondrainiarivelo JP, Razafindrabe T, Derschum H, Silaghi C, Pothmann D, Veit A, Hogan B, May J, Girmann M, Kramme S, Fleischer B, Poppert S.
Ticks Tick Borne Dis. 2016 Feb;7(1):60-5. PMID: 26318262

Diagnosing Salmonella enterica Serovar Typhi Infections by Polymerase Chain Reaction Using EDTA Blood Samples of Febrile Patients From Burkina Faso
Al-Emran HM, Hahn A, Baum J, Cruz Espinoza LM,Deerin J, Im J, Labore LP, Ibrango S, von Kalckreuth V, Konings F, Marks F, Sampo E, Panzner U, Park SE, Pak GD, Schütt-Gerowitt H, Vinnemeier CV, Warren M, Sousa AB
Clin Infect Dis. 2016 Mar 15;62 Suppl 1:S37-41

Diagnostic performance of the Luminex xTAG gastrointestinal pathogens panel to detect rotavirus in Ghanaian children with and without diarrhoea
Leva A, Eibach D, Krumkamp R, Käsmaier J, Rubbenstroth D, Adu-Sarkodie Y, May J, Tannich E, Panning M
Virol J. 2016 Jul 29;13:132. PubMed PMID: 27473598

International CDS Study Group.. Longitudinal course of ante- and postpartum generalized anxiety symptoms and associated factors in West-African women from Ghana and Côte d'Ivoire
Barthel D, Kriston L, Barkmann C, Appiah-Poku J, Te Bonle M, Esther Doris KY, Carine Esther BK, Jean Armel KE, Mohammed Y, Osei Y, Fordjour D, Owusu D, Eberhardt KA, Hinz R, Koffi M, N'Goran E, Nguah SB, Tagbor H, Schoppen S, Ehrhardt S, Bindt C
J Affect Disord. 2016 Jun;197:125-33. PubMed PMID: 26991367

Mosquito Passage Dramatically Changes var Gene Expression in Controlled Human Plasmodium falciparum Infections
Bachmann A, Petter M, Krumkamp R, Esen M, Held J, Scholz JA, Li T, Sim BK, Hoffman SL, Kremsner PG, Mordmüller B, Duffy MF, Tannich E
PLoS Pathog. 2016 Apr 12;12(4):e1005538. PubMed PMID: 27070311

Utilizing Moist or Dry Swabs for the Sampling of Nasal MRSA Carriers? An In Vivo and In Vitro Study
Warnke P, Devide A, Weise M, Frickmann H, Schwarz NG, Schaffler H, Ottl P, Podbielski A
PLoS One 2016,11:e0163073

Molecular Epidemiology of Carbapenem-Resistant Acinetobacter Baumannii Complex Isolates from Patients that were Injured During the Eastern Ukrainian Conflict
Granzer H, Hagen RM, Warnke P, Bock W, Baumann T, Schwarz NG, Podbielski A, Frickmann H, Koeller T
Eur J Microbiol Immunol (Bp) 2016,6:109-117

Dengue and chikungunya seroprevalence in Gabonese infants prior to major outbreaks in 2007 and 2010: A sero-epidemiological study
Gabor JJ, Schwarz NG, Esen M, Kremsner PG, Grobusch MP
Travel Med Infect Dis 2016,14:26-31

Risk Reduction of Needle Stick Injuries Due to Continuous Shift from Unsafe to Safe Instruments at a German University Hospital
Frickmann H, Schmeja W, Reisinger E, Mittlmeier T, Mitzner K, Schwarz NG, Warnke P, Podbielski A
Eur J Microbiol Immunol (Bp) 2016,6:227-237

Prevalence and genetic characterization of Anaplasma marginale in zebu cattle (Bos indicus) and their ticks (Amblyomma variegatum, Rhipicephalus microplus) from Madagascar
Pothmann D, Poppert S, Rakotozandrindrainy R, Hogan B, Mastropaolo M, Thiel C, Silaghi
Ticks Tick Borne Dis. 2016 Oct;7(6):1116-1123. PubMed ID: 27592064

Prospective, consecutive case series of 158 snakebite patients treated at Savannakhet provincial hospital, Lao People's Democratic Republic with high incidence of anaphylactic shock to horse derived F(ab')2 antivenom
Vongphoumy I., Chantilat P., Vilayvong P., Blessmann J
Toxicon. 2016 Jul;117:13-21. doi: 10.1016/j.toxicon.2016.03.011. Epub 2016 Mar 16

Selection of Plasmodium falciparum pfcrt and pfmdr1 polymorphisms after treatment with artesunate-amodiaquine fixed dose combination or artemether-lumefantrine in Liberia
Otienoburu SD, Maïga-Ascofaré O, Schramm B, Jullien V, Jones JJ, Zolia YM, Houzé P, Ashley EA, Kiechel JR, Guérin PJ, Le Bras J, Houzé S
Malar J. 2016 Sep 5;15:452. PubMed PMID: 27596849

2015

Group B Streptococci Serotype Distribution in Pregnant Women in Ghana: Assessment of Potential Coverage Through Future Vaccines
Vinnemeier CD, Brust P, Owusu-Dabo E, Sarpong N, Sarfo EY, Bio Y, Rolling T, Dekker D, Adu-Sarkodie Y, Eberhardt KA, May J, Cramer JP.
Trop Med Int Health. 2015 Nov;20(11):1516-1524. PMID: 26285044

High detection rate of Rickettsia africae in Amblyomma variegatum but low prevalence of anti-rickettsial antibodies in healthy pregnant women in Madagascar
Keller C, Krüger A, Schwarz NG, Rakotozandrindrainy R, Rakotondrainiarivelo JP, Razafindrabe T, Derschum H, Silaghi C, Pothmann D, Veit A, Hogan B, May J, Girmann M, Kramme S, Fleischer B, Poppert S.
Ticks Tick Borne Dis. 2015 Aug 18. pii: S1877-959X(15)30003-0. [Epub ahead of print] PMID: 26318262

Molecular characterization of Cryptosporidium spp. Among children in rural Ghana.
Eibach D, Krumkamp R, Al-Emran HM, Sarpong N, Hagen RM, Adu-Sarkodie Y, Tannich E, May J.
PLoS Negl Trop Dis. 2015 Mar 6;9(3):e0003551. PMID: 25749411

Identification of nasal colonization with β-lactamase-producing Enterobacteriaceae in patients, health care workers and students in Madagascar
Micheel V, Hogan B, Rakotoarivelo RA, Rakotozandrindrainy R, Razafimanatsoa F, Razafindrabe T, Rakotondrainiarivelo JP, Crusius S, Poppert S, Schwarz NG, May J, Frickmann H, Hagen RM.
Eur J Microbiol Immunol (Bp). 2015 Mar;5(1):116-25. PMID: 25908994

Correction: Gastrointestinal Infections and Diarrheal Disease in Ghanaian Infants and Children: An Outpatient Case-Control Study
Krumkamp R, Sarpong N, Schwarz NG, Adlkofer J, Loag W, Eibach D, Hagen RM, Adu-Sarkodie Y, Tannich E, May J.
PLoS Negl Trop Dis. 2015 Apr 22;9(4):e0003728. PMID: 25901599

Drinking water from dug wells in rural ghana--salmonella contamination, environmental factors, and genotypes
Dekker DM, Krumkamp R, Sarpong N, Frickmann H, Boahen KG, Frimpong M, Asare R, Larbi R, Hagen RM, Poppert S, Rabsch W, Marks F, Adu-Sarkodie Y, May J.
Int J Environ Res Public Health. 2015 Mar 27;12(4):3535-46. PMID: 25826395

Clinical Indicators for Bacterial Co-Infection in Ghanaian Children with P. falciparum Infection
Nielsen MV, Amemasor S, Agyekum A, Loag W, Marks F, Sarpong N, Dekker D, Adu-Sarkodie Y, May J.
PLoS ONE. 2015 Apr 9;10(4):e0122139. PMID: 25856341

PCR for enteric pathogens in high-prevalence settings. What does a positive signal tell us?
Frickmann H, Schwarz NG, Rakotozandrindrainy R, May J, Hagen RM.
Infect Dis (Lond). 2015 Jul;47(7):491-8. PMID: 25761823

Long-term live imaging reveals cytosolic immune responses of host hepatocytes against Plasmodium infection and parasite escape mechanisms
Prado M, Eickel N, De Niz M, Heitmann A, Agop-Nersesian C, Wacker R, Schmuckli-Maurer J, Caldelari R, Janse CJ, Khan SM, May J, Meyer CG, Heussler VT.
Autophagy. 2015 Jul 24:0. [Epub ahead of print] PMID: 26208778

Molecular epidemiology and seroprevalence in asymptomatic Plasmodium falciparum infections of Malagasy pregnant women in the highlands
Maïga-Ascofaré O, Rakotozandrindrainy R, Girmann M, Hahn A, Randriamampionona N, Poppert S, May J, Schwarz NG.
Malar J. 2015 May 3;14:188. PMID: 25935753

Prevalence of malaria parasitaemia in school children from two districts of Ghana earmarked for indoor residual spraying: a cross-sectional study
Sarpong N, Owusu-Dabo E, Kreuels B, Fobil JN, Segbaya S, Amoyaw F, Hahn A, Kruppa T, May J.
Malar J. 2015 Jun 25;14:260. PMID: 26109461

Is A578S SNP in K13-propeller a marker of emerging Plasmodium falciparum artemisinin-resistance in Africa?
Maïga-Ascofaré OM, May J.
J Infect Dis. 2015 Aug 12. [Epub ahead of print] PMID: 26268852

16S rRNA Gene Sequence-Based Identification of Bacteria in Automatically Incubated Blood Culture Materials from Tropical Sub-Saharan Africa
Frickmann H, Dekker D, Schwarz NG, Hahn A, Boahen K, Sarpong N, Adu-Sarkodie Y, Halbgewachs E, Marks F, von Kalckreuth V, Poppert S, Loderstaedt U, May J, Hagen RM.
PLoS ONE. 2015 Aug 13;10(8):e0135923. PMID: 26270631

Epidemiology of Ebola virus disease and of other highly contagious, life-threatening diseases with low incidence in Germany
Ehlkes L, Kreuels B, Schwarz NG, May J.
Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2015 Jul;58(7):705-13. German. PMID: 25997608

Gastrointestinal infections and diarrheal disease in Ghanaian infants and children: an outpatient case-control study
Krumkamp R, Sarpong N, Schwarz NG, Adlkofer J, Loag W, Eibach D, Hagen RM, Adu-Sarkodie Y, Tannich E, May J.
PLoS Negl Trop Dis. 2015 Mar 4;9(3):e0003568

Microbiological laboratory diagnostics of neglected zoonotic diseases (NZDs)
Schwarz, N.G., et al., Schwarz NG, Loderstaedt U, Hahn A, Hinz R, Zautner AE, Eibach D, Fischer M, Hagen RM, Frickmann H
Acta Trop. 2015 Sep 21

Urbanicity and Paediatric Bacteraemia in Ghana—A Case-Control Study within a Rural- Urban Transition Zone
Sothmann P, Krumkamp R, Kreuels B, Sarpong N, Frank C, Ehlkes L, Fobil J, Gyau K, Jaeger A, Bosu B, Marks F, Owusu-Dabo E, Salzberger B, May J
PLoS One. 2015 Sep 29;10(9):e0139433. eCollection 2015. PMID: 26418004

Screening agars for MRSA: evaluation of a stepwise diagnostic approach with two different selective agars for the screening for methicillin-resistant Staphylococcus aureus (MRSA)
Micheel V, Hogan B, Köller T, Warnke P, Crusius S, Hinz R, Hagen RM, Schwarz NG, Frickmann H
Mil Med Res. 2015 Jul 21;2:18. doi: 10.1186/s40779-015-0046-1. eCollection 2015

Surveillance and Outbreak Response Management System (SORMAS) to support the control of the Ebola virus disease outbreak in West Africa
Fähnrich C, Denecke K, Adeoye OO, Benzler J, Claus H, Kirchner G, Mall S, Richter R, Schapranow MP, Schwarz N, Tom-Aba D, Uflacker M, Poggensee G, Krause G
Euro Surveill. 2015;20(12):pii=21071

Influenza A and Parvovirus B19 Seropositivity Rates in Gabonese Infants
Gabor JJ, Schwarz NG, Esen M, Kremsner PG, Grobusch MP
The American journal of tropical medicine and hygiene 93:407-409

Seuchen – gestern, heute, morgen
Ehkles L, May J
APuZ 20-21/2015

Full matching approach to instrumental variables estimation with application to the effect of malaria on stunting
Kang H, Kreuels B, May J, Small DS.
Annals of Applied Statistics. 2015:1-62

2014

Mobile phone-based mHealth approaches for public health surveillance in sub-Saharan Africa: a systematic review
Brinkel J, Krämer A, Krumkamp R, May J, Fobil J.
Int J Environ Res Public Health. 2014 Nov 12;11(11):11559-82. PMID: 25396767

Cyclovirus CyCV-VN species distribution is not limited to Vietnam and extends to Africa
Garigliany MM, Hagen RM, Frickmann H, May J, Schwarz NG, Perse A, Jöst H, Börstler J, Shahhosseini N, Desmecht D, Mbunkah HA, Daniel AM, Kingsley MT, de Mendonca Campos R, de Paula VS, Randriamampionona N, Poppert S, Tannich E, Rakotozandrindrainy R, Cadar D, Schmidt-Chanasit J.
Sci Rep. 2014 Dec 18;4:7552. PMID: 25518947.

Endothelial protein C receptor gene variants not associated with severe malaria in ghanaian children
Schuldt K, Ehmen C, Evans J, May J, Ansong D, Sievertsen J, Muntau B, Ruge G, Agbenyega T, Horstmann RD.
PLoS One. 2014 Dec 26;9(12):e115770. PMID: 25541704.

Delayed hemolysis after treatment with parenteral artesunate in African children with severe malaria - a double-center prospective study
Rolling T, Agbenyega T, Issifou S, Adegnika AA, Sylverken J, Spahlinger D, Ansong D, Löhr SJZ, Burchard GD, May J, Mordmüller B, Krishna S, Kremsner PG, P Cramer J
J Infect Dis. 2014 Jun 15;209(12):1921-8

Evidence of promiscuous endothelial binding by Plasmodium falciparum-infected erythrocytes
Esser C, Bachmann A, Kuhn K, Schuldt K, Förster B, Bente M, May J, Koch-Nolte F, Yanez-Mo M, Sánchez-Madrid F, Schinkel AH, Jalkanen S, Craig AG, Horstmann RD
Cell Microbiol (2014)

Schistosoma mansoni in schoolchildren in a Madagascan highland school assessed by PCR and sedimentation microscopy and Bayesian estimation of sensitivities and specificities
Schwarz NG, Rakotozandrindrainy R, Heriniaina JN, Randriamampionona N, Hogan B, Frickmann H, Dekker D, Poppert S, Razafindrabe T, Rakotondrainiarivelo JP, May J, Hagen R
Acta Tropica 134:89-94 (2014)

International CDS Study Group. Mental health related determinants of parenting stress among urban mothers of young children - results from a birth-cohort study in Ghana and Côte d'Ivoire
Guo N, Bindt C, Te Bonle M, Appiah-Poku J, Tomori C, Hinz R, Barthel D, Schoppen S, Feldt T, Barkmann C, Koffi M, Loag W, Nguah SB, Eberhardt KA, Tagbor H, Bass JK, N'Goran E, Ehrhardt S
BMC Psychiatry 14:156 1-12 (2014)

Geographically weighted Poisson Regression of Land Use/Land Cover Determinants of Plasmodium falciparum Transmission
Ehlkes L, Krefis AC, Kreuels B, Krumkamp R, Adjei O, Ayim-Akonor Matilda, Kobbe R, Hahn A, Vinnemeier R, Loag W, Schickhoff U, May J.
Int J Health Geographics 13;35 (2014)

Monitoring parasite diversity for malaria elimination in sub-Saharan Africa
Ghansah A, Amenga-Etego L, Amambua-Ngwa A, Andagalu B, Apinjoh T, Bouyou-Akotet M, Cornelius V, Golassa L, Andrianaranjaka VH, Ishengoma D, Johnson K, Kamau E, Maïga-Ascofaré O, Mumba D, Tindana P, Tshefu-Kitoto A, Randrianarivelojosia M, William Y, Kwiatkowski DP, Djimde AA
Science 345:1297-8 (2014)

A Case of Severe Ebola Virus Infection Complicated by Gram-Negative Septicemia
Kreuels B, Wichmann D, Emmerich P, Schmidt-Chanasit J, de Heer G, Kluge S, Sow A, Renné T, Günther S, Lohse AW, Addo MM, Schmiedel S
N Engl J Med (2014)

2013

Serological survey on HIV and Syphilis in pregnant women in Madagascar
Frickmann H, Schwarz NG, Girmann M, Hagen RM, Poppert S, Crusius S, Podbielski A, Heriniaina JN, Razafindrabe T, Rakotondrainiarivelo JP, May J, Rakotozandrindrainy R.
Trop Med Int Health. 2013 Jan;18(1):35-9. doi: 10.1111/tmi.12007. Epub 2012 Oct 24.

Genome-wide association study indicates two novel resistance loci for severe malaria
Timmann C, Thye T, Vens M, Evans J, May J, Ehmen C, Sievertsen J, Muntau B, Ruge G, Loag W, Ansong D, Antwi S, Asafo-Adjei E, Nguah SB, Kwakye KO, Akoto AO, Sylverken J, Brendel M, Schuldt K, Loley C, Franke A, Meyer CG, Agbenyega T, Ziegler A, Horstmann RD
Nature. 2012 Sep 20;489(7416):443-6. doi: 10.1038/nature11334. Epub 2012 Aug 15.

Increased detection of invasive enteropathogenic bacteria in pre-incubated blood culture materials by real-time PCR in comparison with automated incubation in Subsaharan Africa
Frickmann H, Dekker D, Boahen K, Acquah S, Sarpong N, Adu-Sarkodie Y, Schwarz NG, May J, Marks F, Poppert S, Wiemer DF, Hagen RM.
Scand J Infect Dis. 2013 Aug;45(8):616-22. doi: 10.3109/00365548.2013.777777. Epub 2013 Apr 3.

The Causal Effect of Malaria on Stunting: A Mendelian Randomization and Matching Approach
Kang H, Kreuels B, Adjei O, Krumkamp R, May J, Small D.
Int J Epidemiol. 2013 Oct;42(5):1390-8

Fluorescence-in-situ-hybridization (FISH) for rapid identification of Salmonella spp. from agar and blood culture broth
Frickmann H, Dekker D, Boahen K, Sarpong N, Adu-Sarkodie Y, Hänle A, Essig A, Schwarz NG, May J, Poppert S, Hagen RM.
Int J Med Microbiol. 2013 Jul;303(5):277-84. doi: 10.1016/j.ijmm.2013.04.001. Epub 2013 Apr 11.

No association between antenatal common mental disorders in low-obstretic risk woman and adverse birth outcomes in their offspring: results from the CDS study in Ghana and Cote D’Ivoire
Bindt C, Guo N, Bonle MT, Appiah-Poku J, Hinz R, Barthel D, Schoppen S, Feldt T, Barkmann C, Koffi M, Loag W, Nguah SB, Eberhardt KA, Tagbor H, N'goran E, Ehrhardt S, International CDS Study Group.
PLoS One. 2013 Nov 18;8(11):e80711. doi: 10.1371/journal.pone.0080711. eCollection 2013.

Association of antepartum and postpartum depression in Ghanaian and Ivorian women with febrile illness in their offspring: A prospective birth cohort study
Guo N, Bindt C, Te Bonle M, Appiah-Poku J, Hinz R, Barthel D, Koffi M, Posdzich S, Deymann S, Barkmann C, Schlüter L, Jaeger A, Blay Nguah S, Eberhardt KA, N'goran E, Tagbor H, Ehrhardt S, International CDS Study Group.
Am J Epidemiol. 2013 Nov 1;178(9):1394-402. doi: 10.1093/aje/kwt142. Epub 2013 Sep 7.

Symptom severity and health care utilisation from children in rural Ghana – A cross-sectional survey
Krumkamp R, Sarpong N, Kreuels B, Ehlkes L, Loag W, Schwarz N, Zeeb H, Adu-Sarkodie Y, May J.
PLoS One. 2013 Nov 14;8(11):e80598. doi: 10.1371/journal.pone.0080598. eCollection 2013.

Malaria – nur ein Problem der Tropen?
Schweikart J, May J.
HygMed 2013; 38 [9]: 366–371

Clinical relevance of different biomarkers in imported plasmodium falciparum malaria in adults: a case control study
Stauga S, Hahn A, Brattig NW, Fischer-Herr J, Baldus S, Burchard GD, Cramer JP.
Malar J. 2013 Jul 16;12:246. doi: 10.1186/1475-2875-12-246.

Food and Drinking Water Hygiene and Intestinal Protozoa in deployed Germany soldiers
Frickmann H, Schwarz NG, Wiemer DF, Fischer M, Tannich E, Scheid PL, Müller M, Schotte U, Bock W, Hagen RM.
Eur J Microbiol Immunol (Bp). 2013 Mar;3(1):53-60. doi: 10.1556/EuJMI.3.2013.1.8. Epub 2013 Mar 13.

Porcine Hokovirus in domestic pigs, Cameroon
Adlhoch C, Kaiser M. Kingsley MT, Schwarz NG, Ulrich M, de Paula VS, Löwa A, Achukwi MD, Poppert S, Schmidt-Chanasit J, Ellerbrok H.
Emerg Infect Dis. 2013 Dec;19(12):2060-2. doi: 10.3201/eid1912.130891.

Influence of parasite density and sample storage time on the reliability of Entamoeba histolytica-specific PCR from formalin-fixed and paraffin-embedded tissues
Frickmann H, Tenner-Racz K, Eggert P, Schwarz NG, Poppert S, Tannich E, Hagen RM.
Diagn Mol Pathol. 2013 Dec;22(4):236-44. doi: 10.1097/PDM.0b013e3182936936.

Systematic review of maritime field studies about stress and strain in seafaring
Oldenburg, M., Hogan, B. & Jensen, H.J.
Int Arch Occup Environ Health. 2013 Jan;86(1):1-15. doi: 10.1007/s00420-012-0801-5. Epub 2012 Aug 23.

2012

Genome-wide association study indicates two novel resistance loci for severe malaria
Timmann C, Thye T, Vens M, Evans J, May J, Ehmen C, Sievertsen J, Muntau B, Ruge G, Loag W, Ansong D, Antwi S, Asafo-Adjei E, Nguah SB, Kwakye KO, Akoto AO, Sylverken J, Brendel M, Schuldt K, Loley C, Franke A, Meyer CG, Agbenyega T, Ziegler A, Horstmann RD
Nature. 2012 Sep 20;489(7416):443-6. doi: 10.1038/nature11334. Epub 2012 Aug 15.

Incidence and Characteristics of Bacteremia among Children in Rural Ghana
Nielsen MV, Sarpong N, Krumkamp R, Dekker D, Loag W, Amemasor S, Agyekum A, Marks F, Huenger F, Krefis AC, Hagen RM, Adu-Sarkodie Y, May J, Schwarz NG.
PLoS One. 2012;7(9):e44063. doi: 10.1371/journal.pone.0044063. Epub 2012 Sep 10.

Extrapolating respiratory tract infection incidences to a rural area of Ghana using a probability model for hospital attendance
Krumkamp R, Schwarz NG, Sarpong N, et al.
Int J Infect Dis. 2012 Jun;16(6):e429-35. doi: 10.1016/j.ijid.2012.02.003. Epub 2012 Apr 5.

Seroprevalence sampling of chikungunya, dengue and Rift Valley fever following a fever outbreak in Eastern Madagascar
Schwarz NG, Girmann M, Randriamampionona N, Bialonski A, Maus D, Krefis AC, Njarasoa C, Rajanalison JF, Ramandrisoa HD, Randriarison ML, May J, Schmidt-Chanasit J, Rakotozandrindrainy R.
Emerg Infect Dis. 2012 Nov;18(11):1780-6. doi: 10.3201/eid1811.111036.

Predictive Value of Fever and Palmar Palllor for P. falciparum parasitaemia in Children from an Endemic Area
Vinnemeier CD, Schwarz NG, Sarpong N, Loag W, Acquah S, Nkrumah B, Hünger F, Adu-Sarkodie Y, May J.
PLoS One. 2012;7(5):e36678. doi: 10.1371/journal.pone.0036678. Epub 2012 May 4.

Area Based Socioeconomic Conditions and Urban Malaria and Diarrhea Mortalities in Accra, Ghana
Fobil JN, Loag W, Schwarz N, Rodrigues F, Meyer CG, Kraemer A, May J.
Int J of Trop Med 7(1): 6-16

Genome-wide association study identifies resistance loci at ATP2B4 and MARVELD3 for severe falciparum malaria
Timmann C, Thye T, Vens M, Evans J, May J, Ehmen C, Sievertsen J, Muntau B, Ruge G, Loag W, Ansong D, Antwi S, Asafo-Adjei E, Nguah SB, Kwakye KO, Akoto AOY, Sylverken J, Brendel M, Schuldt K, Loley C, Franke A, Meyer CG, Agbenyega T, Ziegler A, Horstmann RD.
Nature. 2012 Sep 20;489(7416):443-6. doi: 10.1038/nature11334. Epub 2012 Aug 15.

Mapping urban malaria and diarrhoea mortality in Accra, Ghana: Evidence of vulnerabilities and implications for urban health policy
Fobil JN, Levers C, Lakes T, Loag W, Kraemer A, May J.
J Urban Health. 2012 Dec;89(6):977-91. doi: 10.1007/s11524-012-9702-x.

Detection of Human Parvovirus 4 in respiratory, faecal and plasma samples from West-African children
Drexler JF, Reber U, Muth D, Herzog P, Annan A, Ebach F, Sarpong N, Acquah S, Adu-Sarkodie S, Panning M, Tannich E, May J, Drosten D, Eis-Hübinger.
Emerg Infect Dis 2012 Oct;18(10):1650-3. doi: 10.3201/eid1810.111373

Human Parvovirus 4 viremia in young children, Ghana
May J, Drexler JF, Reber U, Sarpong N, Acquah S, Panning M, Drosten C, Eis-Hübinger AM.
Emerg Infect Dis. 2012 Oct;18(10):1690-2. doi: 10.3201/eid1810.111836.

Antigen specific IL-2 secretion correlates with NK cell responses after immunization of Tanzanian children with the RTS, S/ASO1 malaria vaccine
Horowitz A. Hafalla J., King E., Lusingu J., Dekker D, Leach A., Moris P., Cohen J., Vekemans J., Villafana T., Corran P., Bejon P., Drakely C., von Seidlein L., Riley E.
J Immunol. 2012 May 15;188(10):5054-62. doi: 10.4049/jimmunol.1102710. Epub 2012 Apr 13.

2011

Diagnostic Accuracy of Organ-Based Dissection Autopsies: A Study of Autopsy Procedures in Ghana
Fobil J, Kraemer A, May J.
J Public Health Africa 2:16-20

Neighborhood urban environmental quality conditions are likely to drive malaria and diarrhea mortality in Accra, Ghana
Fobil JN, Kraemer A, Meyer CG, May J.
J Environ Public Health. 2011;2011:484010. doi: 10.1155/2011/484010. Epub 2011 Jun 21.

Highly co-ordinated var gene expression and switching in clinical Plasmodium falciparum isolates from non-immune malaria patients
Bachmann A, Predehl S, May J, Harder S, Burchard GD, Gilberger TW, Tannich E, Bruchhaus I.
Cell Microbiol. 2011 Sep;13(9):1397-409. doi: 10.1111/j.1462-5822.2011.01629.x. Epub 2011 Jul 11.

Malaria transmission in two rural communities in the forest zone of Ghana
Abonuusum A, Owusu-Daako K, Tannich E, May J, Garms R, Kruppa T.
Parasitol Res. 2011 Jun;108(6):1465-71. doi: 10.1007/s00436-010-2195-1. Epub 2010 Dec 14.

A -436C A polymorphism in the human FAS gene promoter associated with severe childhood malaria
Schuldt K, Kretz CC, Timmann C, Sievertsen J, Ehmen C, Esser C, Loag W, Ansong D, Dering C, Evans J, Ziegler A, May J, Krammer PH, Agbenyega T, Horstmann RD.
PLoS Genet. 2011 May;7(5):e1002066. doi: 10.1371/journal.pgen.1002066. Epub 2011 May 19.

Comparative evaluation of two rapid field tests for malaria diagnosis: Partec Rapid Malaria Test® and Binax Now® Malaria Rapid Diagnostic Test
Nkrumah B, Acquah SE, Ibrahim L, May J, Brattig N, Tannich E, Nguah SB, Adu-Sarkodie Y, Huenger F.
BMC Infect Dis. 2011 May 23;11:143. doi: 10.1186/1471-2334-11-143.

ABO blood group and the risk of placental malaria in sub-Saharan Africa
Adegnika AA, Luty AJ, Grobusch MP, Ramharter M, Yazdanbakhsh M, Kremsner PG, Schwarz NG.
Malar J. 2011 Apr 22;10:101. doi: 10.1186/1475-2875-10-101.

Hemoglobin estimation by the HemoCue(R) portable hemoglobin photometer in a resource poor setting
Nkrumah B, Nguah SB, Sarpong N, Dekker D, Idriss A, May J, Adu-Sarkodie Y.
BMC Clin Pathol. 2011 Apr 21;11:5. doi: 10.1186/1472-6890-11-5.

IL3 variant on chromosomal region 5q31-33 and protection from recurrent malaria attacks
Meyer CG, Fernandez MC, Intemann C, Kreuels B, Kobbe R, Kreuzberg C, Ayim M, Rüther A, Apiah-Thompson P, Agyim B, Loag W, Ehmen C, Adjei, S, Adjei O, May J.
Hum Mol Genet. 2011 Mar 15;20(6):1173-81. doi: 10.1093/hmg/ddq562. Epub 2011 Jan 10.

Spatial analysis of land cover determinants of malaria incidence in the ashanti region, ghana
Krefis AC, Schwarz NG, Nkrumah B, Acquah S, Loag W, Oldeland J, Sarpong N, Adu-Sarkodie Y, Ranft U, May J.
PLoS One. 2011 Mar 23;6(3):e17905. doi: 10.1371/journal.pone.0017905.

Modeling the relationship between precipitation and malaria incidence in children from a holoendemic area in Ghana
Krefis AC, Schwarz NG, Krüger A, Fobil J, Nkrumah B, Acquah S, Loag W, Sarpong N, Adu-Sarkodie Y, Ranft U, May J.
Am J Trop Med Hyg. 2011 Feb;84(2):285-91. doi: 10.4269/ajtmh.2011.10-0381.

Follow-up survey of children who received sulfadoxine-pyrimethamine for intermittent preventive antimalarial treatment in infants
Kobbe R, Hogan B, Adjei S, Klein P, Kreuels B, Loag W, Adjei O, May J.
J Infect Dis. 15;203(4):556-60

Malaria transmission in two rural communities in the forest zone of Ghana
Abonuusum A, Owusu-Daako K, Tannich E, May J, Garms R, Kruppa T.
Parasitol Res. 2011 Jun;108(6):1465-71. doi: 10.1007/s00436-010-2195-1. Epub 2010 Dec 14.

2010

Differing effects of HbS and HbC traits on uncomplicated falciparum malaria, anemia and child growth
Kreuels B, Kreuzberg C, Kobbe R, Ayim-Akonor M, Apiah-Thompson P, Thomson B, Ehmen C, Adjei S, Langefeld I, Adjei O, May J.
Blood. 2010 Jun 3;115(22):4551-8. doi: 10.1182/blood-2009-09-241844. Epub 2010 Mar 15.

Systemic bacteraemia in children presenting with clinical pneumonia and the impact of non-typhoid salmonella (NTS)
Schwarz NG, Sarpong N, Hünger F, Marks F, Acquah SE, Agyekum A, Nkrumah B, Loag W, Hagen RM, Evans JA, Dekker D, Fobil JN, Meyer CG, May J, Adu-Sarkodie Y.
BMC Infect Dis. 2010 Nov 4;10:319. doi: 10.1186/1471-2334-10-319.

Multiplicity of Plasmodium falciparum infection following intermittent preventive treatment in infants
Buchholz U, Kobbe R, Danquah I, Zanger P, Reither K, Abruquah HH, Grobusch MP, Ziniel P, May J, Mockenhaupt FP.
Malar J. 2010 Aug 26;9:244. doi: 10.1186/1475-2875-9-244.

Assessing the relationship between socioeconomic conditions and urban environmental quality in Accra, Ghana
Fobil J, May J, Kraemer A.
Int J Environ Res Public Health. 2010 Jan;7(1):125-45. doi: 10.3390/ijerph7010125. Epub 2010 Jan 13.

FcGR2A functional genetic variant associated with susceptibility to severe malarial anaemia in Ghanaian children
Schuldt K, Esser C, Evans J, May J, Timmann C, Ehmen C, Loag W, Ansong D, Ziegler A, Agbenyega T, Meyer CG, Horstmann RD.
J Med Genet. 2010 Jul;47(7):471-5. doi: 10.1136/jmg.2009.073643. Epub 2009 Dec 3.

Surveillance of malaria drug resistance: improvement needed?
Kun FJ, May J, Noedl H.
Therapy 7:3-6 [Editorial]

Novel human parvovirus 4 genotype 3 in infants, Ghana
Panning M, Kobbe R, Vollbach S, Drexler JF, Adjei S, Adjei O, Drosten C, May J, Eis-Hübinger AM.
Emerg Infect Dis. 2010 Jul;16(7):1143-6. doi: 10.3201/eid1607.100025.

Comparison of the novel Partec rapid malaria test to the conventional Giemsa stain and the gold standard real-time PCR
Nkrumah B, Agyekum A, Acquah SE, May J, Tannich E, Brattig N, Nguah SB, von Thien H, Adu-Sarkodie Y, Hünger F.
J Clin Microbiol. 2010 Aug;48(8):2925-8. doi: 10.1128/JCM.02403-09. Epub 2010 Jun 16.

Principal component analysis of socioeconomic and their associoation with malaria in children from the Ashanti Region, Ghana
Krefis AC, Schwarz NG, Nkrumah B, Acquah S, Loag W, Sarpong N, Adu-Sarkodie Y, Ranft U, May J.
Malaria Journal 2010, 9:201. doi:10.1186/1475-2875-9-201.

Typhoid Fever among Children, Ghana
Marks F, Adu-Sarkodie Y, Huenger F, Sarpong N, Ekuban S, Agyekum A, Nkrumah S, Schwarz NG, Favorov M, Meyer CG, May J.
Emerg Infect Dis 16(11):1796-7. doi: 10.3201/eid1611.100388.

National health insurance coverage and socio-economic status in a rural district of Ghana
Sarpong N, Loag W, Fobil J, Meyer CG, Adu-Sarkodie Y, May J, Schwarz NG.
Trop Med Int Health. 2010 Feb;15(2):191-7. doi: 10.1111/j.1365-3156.2009.02439.x. Epub 2009 Dec 4.

Mumps outbreak in the Republic of Moldova, 2007-2008
Schwarz NG, Bernard H, Melnic A, Bucov V, Caterinciuc N, Heiden MAD, Andrews N, Pebody R, Aidyralieva C, Hahné S.
Pediatr Infect Dis J. 2010 Aug;29(8):703-6. doi: 10.1097/INF.0b013e3181d743df.

WHO guidelines for antimicrobial treatment in children admitted to hospital in an area of intense Plasmodium falciparum transmission: prospective study
Nadjm B, Amos B, Mtove G, Ostermann J, Chonya S, Wangai H, Kimwera J, Msuya W, Mtei F, Dekker D, Malahiyo R, Olomi R, Crump J, Whitty C, Reyburn H
BMJ. 2010 Mar 30;340:c1350. doi: 10.1136/bmj.c1350

Safety of the malaria vaccine candidate, RTS,S/AS01E in 5 to 17 month old Kenyan and Tanzanian Children
Lusingu J, Olotu A, Leach A, Lievens M, Vekemans J, Olivier A, Benns S, Olomi R, Msham S, Lang T, Gould J, Hallez K, Guerra Y, Njuguna P, Awuondo KO, Malabeja A, Abdul O, Gesase S, Dekker D, Malle L, Ismael S, Mturi N, Drakeley CJ, Savarese B, Villafana T, Ballou WR, Cohen J, Riley EM, Lemnge MM, Marsh K, Bejon P, von Seidlein L
PLoS One. 2010 Nov 29;5(11):e14090. doi: 10.1371/journal.pone.0014090

2009

Efficacy and safety of intermittent preventive treatment with sulfadoxine-pyrimethamine for malaria in African infants: a pooled analysis of six randomised, placebo-controlled trials
Aponte JJ, Schellenberg D, Egan A, Breckenridge A, Carneiro I, Critchley J, Danquah I, Dodoo A, Kobbe R, Lell B, May J, Premji Z, Sanz S, Sevene E, Soulaymani-Becheikh R, Winstanley P, Adjei S, Anemana S, Chandramohan D, Issifou S, Mockenhaupt F, Owusu-Agyei S, Greenwood B, Grobusch MP, Kremsner PG, Macete E, Mshinda H, Newman RD, Slutsker L, Tanner M, Alonso P, Menendez C.
Lancet. 2009 Oct 31;374(9700):1533-42. doi: 10.1016/S0140-6736(09)61258-7. Epub 2009 Sep 16.

Risk factors for human infection with Puumala virus, southwestern Germany
Schwarz AC, Ranft U, Piechotowski I, Childs JE, Brockmann SO.
Emerg Infect Dis. 2009 Jul;15(7):1032-9. doi: 10.3201/eid1507.081413.

Reasons for non-adherence to vaccination at mother and child care clinics (MCCs) in Lambarene, Gabon
Schwarz NG, Gysels M, Pell C, Gabor J, Schlie M, Issifou S, Lell B, Kremsner K, Grobusch MP, Pool R.
Vaccine. 2009 Aug 27;27(39):5371-5. doi: 10.1016/j.vaccine.2009.06.100. Epub 2009 Jul 17.

Malaria transmission in non-endemic areas: case report, review of the literature and implications for public health management.
Zoller T, Naucke TJ, May J, Hoffmeister B, Flick H, Williams CJ, Frank C, Bergmann F, Suttorp N, Mockenhaupt FP.
Malar J. 2009 Apr 20;8:71. doi: 10.1186/1475-2875-8-71.

Sickle cell trait (HbAS) and stunting in children below two years of age in an area of high malaria transmission
Kreuels B, Ehrhardt S, Kreuzberg C, Adjei S, Kobbe R, Burchard GD, Ehmen C, Ayim M, Adjei O, May J.
Malar J. 2009 Jan 16;8:16. doi: 10.1186/1475-2875-8-16.

2008

A randomized trial on effectiveness of artemether-lumefantrine versus artesunate plus amodiaquine for unsupervised treatment of uncomplicated Plasmodium falciparum malaria in Ghanaian children
Kobbe R, Klein P, Adjei S, Amemasor S, Thompson WN, Heidemann H, Nielsen MV, Vohwinkel J, Hogan B, Kreuels B, Bührlen M, Loag W, Ansong D, May J.
Malar J. 2008 Dec 19;7:261. doi: 10.1186/1475-2875-7-261.

Therapeutic and prophylactic effect of intermittent preventive anti-malarial treatment in infants (IPTi) from Ghana and Gabon
May J, Adjei S, Kobbe R, Busch W, Gabor JJ, Issifou S, Kreuels B, Lell B, Schwarz NG, Adjei O, Kremsner PG, Grobusch MP.
Malar J. 2008 Oct 1;7:198. doi: 10.1186/1475-2875-7-198.

Simvastatin treatment of Plasmodium berghei infected C57BL/6 mice shows no effect on parasitemia or incidence of cerebral malaria
Kobbe R, Schreiber N, Fleischer B, May J, Jacobs T.
Antimicrob Agents Chemother. 2008 Apr;52(4):1583-4. doi: 10.1128/AAC.01428-07. Epub 2008 Feb 11.

Spatial variation of malaria incidence in young children from a geographically homogeneous area with high endemicity
Kreuels B, Kobbe R, Adjei S, Kreuzberg C, von Reden I, Bäter K, Klug S, Busch W, Meyer CG, Adjei O, May J.
J Infect Dis. 2008 Jan 1;197(1):85-93. doi: 10.1086/524066.

Expression of Plasmodium falciparum 3D7 STEVOR proteins for evaluation of antibody responses following malaria infections in naïve infants
Schreiber N, Khattab A, Petter M, Marks F, Adjei S, May J, Klinkert MQ.
Parasitology. 2008 Feb;135(2):155-67. Epub 2007 Oct 12.

Q fever in young children, Ghana
Kobbe R, Kramme K, Kreuels B, Adjei S, Kreuzberg C, Panning M, Adjei O, Fleischer B, May J.
Emerg Infect Dis. 2008 Feb;14(2):344-6. doi: 10.3201/eid1402.070971.

2007

Malaria incidence and efficacy of intermittent preventive treatment in infants (IPTi)
Kobbe R, Adjei S, Kreuzberg C, Kreuels B, Thompson B, Thompson PA, Marks F, Busch W, Tonsun M, Schreiber N, Opoku E, Adjei O, Meyer CG, May J.
Malaria Journal 2007, 6:163. doi:10.1186/1475-2875-6-163

A Randomized Controlled Trial of Extended Intermittent Preventive Antimalarial Treatment in Infants
Kobbe R, Kreuzberg C, Adjei S, Thompson B, Langefeld I, Abruquah HH, Kreuels B, Ayim M, Busch W, Marks F, Amoah K, Opoku E, Meyer CG, Adjei O, May J.
Clin Infect Dis. 2007 Jul 1;45(1):16-25. Epub 2007 May 29.

Hemoglobin variants and disease manifestations in severe falciparum malaria
May J, Evans JA, Timmann C, Ehmen C, Busch W, Thye T, Agbenyega T, Horstmann RD.
JAMA. 2007 May 23;297(20):2220-6.

Maternal antibodies to domains of a placental Plasmodium falciparum 732var gene are associated with an anti-disease immunity
Khattab A, Chia YS, Deloron P, May J, Klinkert MQ.
Parasitol Res. 2007 Aug;101(3):767-74. Epub 2007 May 23.

Travel-associated Coxiella Burnetii infections: 3 cases of Q fever with different clinical manifestation
Kobbe R, Kramme S, Gocht A, Werner M, Lippert U, May J, Burchard G.
Travel Med Infect Dis. 2007 Nov;5(6):374-9. Epub 2007 Sep 12.

Immune responses after single dose sulfadoxine-pyrimethamine indicates underestimation of protective efficacy of intermittent preventive treatment in infants
Schreiber N, Kobbe R, Adjei S, Adjei O, Klinkert MQ, May J.
Trop Med Int Health. 2007 Oct;12(10):1157-63.

Cross-resistance may prevent return of chloroquine sensitive falciparum malaria [commentary]
Kobbe R, Meyer CG, May J.
N Engl J Med 356:868 (2007)

2006

Seasonal dynamics of Plasmodium falciparum prevlence and high parasite diversity of first infections in infants from central Ghana, West Africa
Kobbe R, Neuhoff, Marks F, Adjei S, Adjei O, Horstmann RD, Meyer CG, May J.
Trop Med Int Health 11:613-9 (2006)

Cerebral malaria is associated with IgG2 and IgG4 antibody responses to recombinant Plasmodium falciparum RIFIN antigen
Schreiber NB, Brattig N, Evans JA, Agbenyega T, Horstmann RD, May J, Klinkert MQ.
Microbes Infect. 2006 Apr;8(5):1269-76. Epub 2006 Feb 6.

Comment: Parasitological rebound effects and emergence of Plasmodium falciparum pyrimethamine resistance by single dose sulfadoxine-pyrimethamine
Marks F, Kobbe R, Meyer CG, May J.
J Infect Dis 193:1609-10 (2006)

Capillary refill time as an independent prognostic indicator in severe and complicated malaria
Evans JA, May J, Ansong D, Antwi S, Asafo-Adjei E, Nguah S, Kwakye KO, Akoto AOY, Owusu Ofori A, Sambian D, Sylverken J, Timmann C, Agbenyega T, Horstmann RD.
J Pediatr. 2006 Nov;149(5):676-81.

Novel promoter polymorphism of the anion exchange protein 1 highly prevalent in Ghana and associated with severe malaria anaemia
von Kalckreuth V, Evans J, Agbenyega T, Horstmann RD, May J.
J Infect Dis. 2006 Oct 1;194(7):949-57. Epub 2006 Aug 30.

Association of HLA haplotypes with PTLD after solid organ transplantation
Subklewe M, Marquis R, Choquet S, Leblond V, Garnier JL, Hetzer R, Swinnen LJ, Oertel S, Papp-Vary M, Gonzalez-Barca E, Hepkema BG, Schoenemann C, May J, Pezzutto A, Riess H.
Transplantation. 2006 Oct 27;82(8):1093-100.

Seasonal variation and high multiplicity of first Plasmodium falciparum infections in children from a holoendemic area in Ghana, West Africa
Kobbe R, Neuhoff, Marks F, Adjei S, Langefeld I, von Reden C, Adjei O, Meyer CG, May J.
Trop Med Int Health. 2006 May;11(5):613-9.

Cerebral malaria is associated with IgG2 and IgG4 antibody responses to recombinant Plasmodium falciparum RIFIN antigen
Schreiber N, Brattig N, Evans J, Tsiri Agbenyega, Horstmann RD, May J, Klinkert MQ.
Microbes Infect. 2006 Apr;8(5):1269-76. Epub 2006 Feb 6.

2005

Parasitological rebound effects and emergence of Plasmodium falciparum pyrimethamine resistance by single dose sulfadoxine-pyrimethamine.
Marks F, von Kalckreuth, Kobbe R, Adjei S, Adjei O, Horstmann RD, Meyer CG, May J.
J Infect Dis. 2005 Dec 1;192(11):1962-5. Epub 2005 Oct 20.

Pre-treatment with chloroquine and parasite chloroquine resistance in Ghanaian children with severe malaria
Evans JA, May J, Tominski D, Eggelte T, Marks F, Abruquah HH, Meyer CG, Timmann C, Agbenyega T, Horstmann RD.
QJM. 2005 Nov;98(11):789-96. Epub 2005 Sep 26.

Antifolates in prevention of HIV-associated opportunistic infections and in intermittent preventive treatment of malaria in Africa
Kobbe R, Marks F, May J, Meyer CG.
Trop Med Int Health 10:1-2 (2005)

High prevalence of markers for sulfadoxine and pyrimethamine resistance of Plasmodium falciparum in the absence of drug pressure in the Ashanti Region of Ghana
Marks F, Evans J, Meyer CG, Browne ENL, Flessner C, von Kalkreuth V, Eggelte T, Agbenyega T, Hoffmann H, Horstmann RD, May J.
Antimicrob Agents Chemother. 2005 Mar;49(3):1101-5.

Polymorphism of TAP1, LMP2 and their common promoter in African children with different manifestations of malaria
Niesporek S, Meyer CG, Kremsner PG, May J.
Int J Immunogenet. 2005 Feb;32(1):7-11.

2004

Genotyping of Plasmodium falciparum pyrimethamine resistance by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS)
Marks F, Meyer CG, Sievertsen J, Timmann C, Evans J, Horstmann RD, May J.
Antimicrob Agents Chemother. 2004 Feb;48(2):466-72.

High mortality of infant bacteraemia clinically indistinguishable from severe malaria
Evans JA, Adusei A, Timmann C, May J, Mack D, Agbenyega T, Horstmann RD, Frimpong E.
QJM. 2004 Sep;97(9):591-7.

Gin Tonic - revisited
Meyer CG, Marks F, May J.
Trop Med Int Health. 2004 Dec;9(12):1239-40.

2003

Association of Plasmodium falciparum chloroquine resistance transporter variant T76 with age-related plasma chloroquine levels
May J, Meyer CG.
Am J Trop Med Hyg. 2003 Feb;68(2):143-6.

A synonymous mutation of ancient origin in the glucose-6-phosphate dehydrogenase gene and assessment of haplotypes
May J, Meyer CG.
Blood Cells Mol Dis. 2003 Jan-Feb;30(1):144-5.

Sensitivity of hemozoin detection by automated flow cytometry in non- and semi-immune malaria patients
Grobusch MP, Hänscheid T, Krämer B, Neukammer J, May J, Seybold J, Kun JFJ, Temmesfeld-Wollbrück B, Kremsner PG, Suttorp N.
Cytometry B Clin Cytom. 2003 Sep;55(1):46-51.

2002

Genetic diversity of Plasmodium falciparum: pre-erythrocytic and asexual blood stages
Meyer CG, May J, Arez AP, Gil JP, do Rosario V.
Trop Med Int Health (2002) 7:395-408. doi: 10.1046/j.1365-3156.2002.00875.x

TNFα-308A associated with shorter intervals of Plasmodium falciparum reinfections
Meyer CG, May J, Lell B, Luty A, Kremsner PG.
Tissue Antigens. 2002 Apr;59(4):287-92.

Infektionserreger als biologische Waffen
Meyer CG, May J.
Anästesiol Intensivmed Notfallmed Schmerzthe 37:538-46 (2002)

2001

HLA-DQB1*0501-restricted Th1-type immune responses to a Plasmodium falciparum liver stage antigen protect from anemia in malarial infection
May J, Lell B, Luty AJF, Meyer CG, Kremsner PG.
J Infect Dis. 2001 Jan 1;183(1):168-72. Epub 2000 Nov 10.

Evidence for a reduced effect of chloroquine against Plasmodium falciparum in α-thalassaemic children
Mockenhaupt FP, May J, Bergqyist Y, Meyer CG, Falusi AG, Bienzle U.
Trop Med Int Health. 2001 Feb;6(2):102-7.

Nitric oxid synthaseLambarene (G-954C), increased nitric oxide production, and protection against malaria
Kun J, Mordmüller B, Perkins DJ, May J, Mercereau-Puijalon O, Alpers M, Weinberg B, Kremsner PG.
J Infect Dis. 2001 Aug 1;184(3):330-6. Epub 2001 Jun 26.

Spleen size determined by ultrasound in patients with AS trait, AC trait and G6PD deficiency in a hyperendemic malaria region (Ashanti Region/Ghana)
Burchard GD, Browne ENL, Sievertsen J, May J, Meyer CG.
Acta Trop. 2001 Oct 22;80(2):103-9.

2000

Red cell glucose-6-phosphat dehydrogenase status and pyruvat kinase activity in a Nigerian population
May J, Meyer CG, Falusi AG, Ademowo OG, Olumese PE, Großterlinden L, Mockenhaupt FP, Luzzatto L, Bienzle U.
Trop Med Int Health. 2000 Feb;5(2):119-23.

Concentrations of chloroquine and malaria parasites in Blood in Nigerian children
Mockenhaupt FP, May J, Bergqvist Y, Ademowo OG, Olumese PE, Falusi AG, Großterlinden L, Meyer CG, Bienzle U.
Antimicrob Agents Chemother. 2000 Apr;44(4):835-9.

Promoter variants of the mannose-binding lectin gene show different binding
Jüliger S, Luckner D, Mordmüller B, May J, Weierich A, Lell B, Luty A, Kremsner PG, Kun JFJ.
Biochem Biophys Res Commun. 2000 Aug 28;275(2):617-22.

Impact of subpatent multi-species and multi-clonal plasmodial infections on anaemia in children from Nigeria
May J, Mockenhaupt F, Ademowo PO, Falusi AG, Olumese P, Bienzle U, Meyer CG.
Trans R Soc Trop Med Hyg 94:399-403 (2000)

Plasma Interleukin-10: Tumor necrosis factor (TNF)-a ratio is associated with TNF promoter variants and predicts malarial complications
May J, Lell B, Luty AJF, Meyer CG, Kremsner PG.
J Infect Dis. 2000 Nov;182(5):1570-3. Epub 2000 Oct 9.

1999

Leishmaniasis. Basic principles, clinical aspects, diagnosis and therapy
Burbach G, May J, Harms G, Bienzle U.
Dtsch Med Wochenschr 124:88-93 (1999)

HLA class II factors associated with Plasmodium falciparum merozoite surface antigen allele families
May J, Meyer CG, Kun JFJ, Lell B, Luckner D, Dippmann AK, Bienzle U, Kremsner PG.
J Inf Dis 179:1042-1045 (1999)

Plasmodium falciparum-infection: Influence on haemoglobin levels in a-thalassaemia and microcytosis
Mockenhaupt FP, Bienzle U, May J, Falusi A, Ademowo OG, Olumese PE, Meyer CG.
J Infect Dis 180:925-928 (1999)

High rate of mixed and subpatent malarial infections in southwest Nigeria
May J, Mockenhaupt F, Ademowo OG, Falusi AG, Olumese PE, Bienzle U, Meyer CG.
Am J Trop Med Hyg 61:339-343 (1999)

Short report: High prevalence and imbalanced age distribution of the Plasmodium falciparum dihydrofolate reductase Asn-108 mutation in an area of low pyrimethamine usage in Nigeria
Mockenhaupt FP, May J, Eggelte TA, Thies FL, Ademowo OG, Bienzle U, Meyer CG.
Am J Trop Med Hyg 61:375-377 (1999)

Tropische Viruserkrankungen
Meyer CG, May J, Schwarz T.
Deut Med Wochenschr 124:1043-1051 (1999)

The contribution of alpha+-thalassaemia to anaemia in a Nigerian population exposed to intense malaria transmission
Mockenhaupt FP, Falusi AG, May J, Ademowo OG, Olumese PE, Meyer CG, Bienzle U.
Trop Med Int Health; 4:302-307 (1999)

Serum transferrin receptor levels in Plasmodium falciparum-infection
Mockenhaupt F, May J, Stark K, Falusi AG, Meyer CG, Bienzle U.
Hematologica 84:869-873 (1999)

The role of red blood cell polymorphisms in resistance and suscebility to malaria
Lell B, May J, Schmidt-Ott R, Lehmann LG, Luckner D, Greve B, Matousek P, Schmid D, Herbich K, Meyer CG, Bienzle U, Kremsner PG.
Clin Infect Dis 28:794-799 (1999)

Association of the ICAM-1Kilifi mutation with protection against severe malaria in Lambaréné, Gabon
Kun JFJ, Klabunde J, Lell B, Luckner D, Alpers M, May J, Meyer CG, Kremsner PG.
Am J Trop Med Hyg 61:776-779 (1999)

1998

Human leucocyte antigens in tuberculosis and leprosy
Meyer CG, May J, Stark K.
Trends Microbiol 6:148-154 (1998)

HLA-DP control of human Schistosoma haematobium infection
May J, Kremsner PG, Milovanovic D, Schnittger L, Löliger CC, Bienzle U, Meyer CG.
Am J Trop Med Hyg 59: 302-306 (1998)

HLA DPA1/DPB1 genotype and haplotype frequencies, and linkage disequilibria in Nigeria, Liberia, and Gabon
May J, Mockenhaupt FP, Löliger CC, Ademowo OG, Falusi AG, Jenisch S, Dippmann AK, Schnittger L, Kremsner PG, Bienzle U, Meyer CG.
Tissue Antigen 52:199-207 (1998)

1997

HLA-DP - part of the concert.
Meyer CG, May J, Schnittger L.
Immunol Today 18:58-61 (1997)

Hepatitis G virus (HGV) RNA and HGV antibodies in renal transplantant recipients: prevalence and risk factors
Stark K, Meyer CG, Tacke M, Schwarz A, Braun C, Huzly D, Engel AM, May J, Bienzle U.
Transplantation 64:608:612 (1997)

HLA-DRB1-DQA1-DQB1 haplotype diversity in in two African populations
Schnittger L, May J, Loeliger CC, Gallin MY, Erttmann KD, Bienzle U, Kremsner PG, Meyer CG.
Tissue Antigens 50:546-551 (1997)

Medikamenten-resistente Malaria
Mockenhaupt F, May J, Bienzle U, Meyer CG.
Dtsch Med Wochenschr 122:1293-1297 (1997)

1996

Met-11 of class II DPa1 first domain associated with onchocerciasis
Meyer CG, Schnittger L, Kretschmer C, May J.
Exp Clin Immunogenet 13:12-19 (1996)

DPB1*BR: an Mhc class II DPB1 allele (DPB1*6601) of negroid origin
Schnittger L, May J, Kretschmer C, Kremsner PG, Meyer CG.
Immunogenetics 44:405-406 (1996)

DPB1*TF, a novel HLA class II DPB1 allele (DPB1*6701) identified in a Turkish family
Meyer CG, May J, Simon C, Böhm BO, Loeliger CC.
Tissue Antigens 48:231-232 (1996)

DPA1*0105, a novel DPA1-variant in a negroid population
May J, Kretschmer C, Schnittger L, Striecker R, Kremsner PG, Meyer CG.
Tissue Antigens 48:593-594 (1996)

1994

Human MHC class II DPB1*02011 and related alleles in West Africa
Meyer CG, Spauke D, May J, Schnittger L.
Mitt Österr Ges Tropenmed Parasitol 16: 43-50 (1994)

DPA1*02012: a DPA1*0201-related Mhc class II allele in West Africa
Meyer CG, May J, Spauke D, Schnittger L.
Immunogenetics 40: 309 (1994)

Cooperations

Arbeitsgruppe May (Infektionsepidemiologie)

Germany

  • University Clinic Hamburg Eppendorf (UKE), Hamburg
  • Instiute for Tropical Medicine, Eberhard Karls University, Tübingen
  • Institut für Virologie, Universität Bonn
  • Helmholtz Centre for Infection Research (HZI), Braunschweig
  • Robert Koch Institut (RKI), Berlin
  • Hasso-Plattner-Institut (HPI), Potsdam
  • Friedrich Loeffler Institut für Medizinische Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald, Greifswald
  • Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Freiburg, Freiburg
  • Institut für Medizinische Mikrobiologie, Virologie und Hygiene, Universitäts-Klinikum Hamburg (UKE), Hamburg
  • Institute of Biochemistry and Molecular Biology, University of Hamburg
  • Institut für Medzinische Mikrobiologie, Universitätsklinikum Gießen, Gießen
  • Institut für Virologie, Philipps-University Marburg
  • Fakultät für Gesundheitswissenschaften, Universität Bielefeld
  • Abteilung für Psychosomatik, Altonaer Kinderkrankenhaus, Universitäts-Klinikum Hamburg (UKE), Hamburg
  • Institute of Public Health (IPH), Universitätsklinikum Heidelberg
  • Leibniz Institute for Prevention Research and Epidemiology (BIPS), Bremen
  • Abteilung für Infektions- und Tropenmedizin, Klinikum der Universität München, München


Europe

  • National Institute for Public Health and the Environment, Bilthoven, The Netherlands
  • Swiss Tropical and Public Health Institute (Swiss TPH), Switzerland
  • Institut für Zellbiologie, Universität Bern, Schweiz
  • National Center of Microbiology, Institute of Health Carlos III, Majadahonda, Madrid, Spain


Africa

  • Kumasi Centre for Collaborative Research (KCCR) (State Agreement), Ghana
  • University of Antananarivo (Memorandum of Understanding), Madgascar
  • Department of Biological, Environmental & Occupational Health Sciences, School of Public Health, University of Ghana, Accra, Ghana
  • Ghana Health Service, Disease Surveillance Service, Accra, Ghana
  • Ghana Health Service, National Public Health & Reference Laboratory (NPHRL), Accra, Ghana
  • VOTO Mobile, Kumasi, Ghana
  • Nigeria Field Epidemiology & Laboratory Training Program (NFELTP), Abuja, Nigeria
  • Federal Ministry of Health (FMOH), Abuja, Nigeria
  • African Field Epidemiology Network (AFENET), Kampala, Uganda
  • WHO Multi Disease Surveillance Centre (MDSC) in Ougadougou, Burkina-Faso
  • Ghana School of Public Health, Accra (GSPH), Ghana
  • Kwame Nkrumah University of Science and Technology (KNUST), Ghana
  • Deutsches Albert- Schweitzer- Zentrum (DASZ), Lambarene, Gabun
  • Centre de Recherche Médicale de Lambaréné (CERMEL), Lambaréné, Gabun
  • Makerere University, School of Public Health, Kampala (MUSPH), Uganda
  • University of Ouagadougou (UO), Burkina-Faso
  • University of Zimbabwe (UZ), Zimbabwe
  • National Institute for Medical Research at Tanga Centre, Tanga (NMRI), Tanzania
  • Muhimbili University of Health and Allied Sciences (MUHAS), Tanzania
  • Ahmadu Bello University (ABU), Zaria, Nigeria


International others

  • International Vaccine Institute (IVI), Seoul, Korea
  • Centers for Disease Control and Prevention (CDC), Atlanta, USA
  • University of Pennsylvania, USA
  • Vietnamese Military Medical University (VMMU), Hanoi, Vietnam
  • Provincial Health Department and Provincial Hospital Savannakhet, Lao PDR
  • Hué University of Medicine and Pharmacy (Hue UMP), Hué city, Vietnam


News from the group

February 2017

"Good to see both sides"

Our research fellow Oumou Maiga-Ascofaré was recently interviewed about her work and
her career path by the German Center for Infection Research (DZIF).
Here is the link to the full interview:
http://www.dzif.de/en/about_us/dzif_people/view/detail/artikel/good_to_see_both_sides/

Oktober 2016

Second GIBACHT cohort finishes series of biosecurity trainer courses in Kampala

The second GIBACHT-cohort completed the Global Partnership Initiated Biosecurity Academia for Controlling Health Threats (GIBACHT) fellowship, a course series including computer learning activities and three on-campus workshops which were held in Hamburg and Berlin (Germany) and Kampala (Uganda).
Development and use of training material is a substantial part of the GIBACHT fellowship, thus making GIBACHT graduates multipliers who can train others in biosecurity and management of catastrophic health events. The final course of the second GIBACHT series took place in Kampala, organized by the African Field Epidemiology Network (AFENET). In the final workshop, GIBACHT fellows implemented their newly developed biosecurity and biosafety relevant case studies with students of the Makerere University (Kampala, Uganda)

May 2016

Genetic determinants for the transmission of Cryptosporidium spp. among humans and animals in Africa

The Infectious Disease Epidemiology working group at the BNITM starts a new project on "Genetic determinants for the transmission of Cryptosporidium spp. among humans and animals in Africa" in May 2016. The project will be funded by the German Research Foundation (DFG) within the "German-African collaborations in infectiology" Initiative. This program aims to investigate neglected infectious diseases in humans and animals as well as to establish research capacities in Africa and to build up inner- African research networks.

Cryptosporidium is a globally distributed single-celled organism that causes persistent diarrhea and malnutrition in immunocompetent individuals as well as severe diarrhea in immunosuppressed patients. Although Cryptosporidium is one of the most common pathogens causing diarrhea in children under the age of two in Africa, a substantial number of cases are thought to remain undiagnosed. The pathogen can be found in both humans and animals. However, there has been very little knowledge yet about the routes of transmission and the reservoirs in Africa. Treatment options have been insufficient.

The objective of this research project is to analyze the genetic diversity of African Cryptosporidium isolates by using whole genome sequencing methods in order to decode chains of transmission and to identify genetic determinants that affect the host specificity. The data will be important to introduce prevention measures and to find potential drug targets. The study will be conducted at four African research sites, in Gabon, Ghana, Madagascar and Tanzania, within a period of three years. The study will be accompanied by an extensive training program for young African scientists to establish a sustainable scientific network in East, West and Central Africa. 

Alumni

Doctoral Thesis

Clemens Frank (2016) : Spatial heterogeneity of malaria in Ghana – a cross-sectional study on the association between urbanicity and the acquisition of immunity (Charité Universitätsmedizin Berlin, gemeinsame Fakultät der Freien Universität und der Humboldt Universität)

Peter Sothmann (2016) : Urbanicity and Paediatric Bacteraemia in Ghana — A Case-Control Study within a Rural-Urban Transition Zone (Universitätsklinikum Regensburg)

Ralf Krumkamp (2016) : Management of childhood infections in rural Ghana – Filling information gaps (University of Bremen)

Hassan Al-Emran (2016) : Molecular Epidemiology of Invasive Salmonella enterica in sub-Sahara Africa (University of Hamburg)

Philipp Klein (2016) : Artemether-Lumefantrin und Artesunat plus Amodiaquin zur Behandlung kindlicher Malaria tropica in Ghana: eine Effizienzstudie (Universität Hamburg)

Christof David Vinnemeier (2015) : Establishment of a clinical algorithm for the diagnosis of P. falciparum malaria in children from an endemic area using a Classification and Regression Tree (CART) model (Medizinischen Fakultät der Georg-August-Universität zu Göttingen)

Maja Verena Nielsen (2015) : Klinische Charakterisierung und Differenzierung von Bakteriämie und Malaria als Ursache schwerer fieberhafter Erkrankungen bei Kindern in Ghana (Universitätsklinikum Eppendorf, Hamburg)

Mirko Girmann (2014) : Seroprevalence of Antibodies against Chikungunya, Dengue and Rift Valley Fever Viruses after Febrile Illness Outbreak, Madagascar

Denise Dekker (2012) : Epidemiology of Salmonella enterica in the Asante Akyem district, Ghana (Universität Hamburg)

Kathrin Bruch (2012) : Einfluss von CD14-Genvarianten auf die Infektionsdynamik von Plasmodium falciparum bei Kleinkindern aus einem Malaria-endemischen Gebiet in Ghana (Universitätsklinikum Eppendorf, Hamburg)

Maria Calixto Fernandez (2012) : Der Einfluss von IL-3-Polymorphismen auf den Zeitpunkt und die Infektionsdynamik der ersten Plasmodien-Infektionen im Leben von Kindern aus einer hyperendemischen Region in Afrika (Universitätsklinikum Eppendorf, Hamburg)

Benedikt Hogan (2012) : Folgeuntersuchung von Kindern nach Einnahme einer intermittierenden präventiven Behandlung der Malaria mit Sulphadoxin-Pyrimethamin (Universitätsklinikum Eppendorf, Hamburg)

Christopher Intemann (2011) : Assoziation humaner Genvarianten mit dem Auftreten von schwerer Malaria im Kindesalter (Medizinische Fakultät der Georg-August-Universität Göttingen)

Julius Fobil (2011) : Spatial urban environmental change and malaria/diarrhoea mortality in Accra, Ghana (Universität Bielefeld)

Anna Caroline Krefis (2011) : Spatial, temporal, and socioeconomic risk factors of malaria in children from the Ashanti Region, Ghana (Medizinische Fakultät der Universität Düsseldorf)

Rieke Katja Neuhoff (2011) : Diagnostik, Prävalenz und Komplexität der Plasmodieninfektion bei drei Monate alten Kindern aus der Ashanti-Region, Ghana (Medizinische Fakultät der Georg-August-Universität Göttingen)

Selinde Merz (2009) : Abhängigkeit der Milzgröße von Plasmodien-Parasitamie, Sichelzellanämie, HbC- Hämoglobinopathie und Glucose-6-Phosphat-Dehydrogenase-Mangel in einem hyperendemischen Malariagebiet Ghanas (Universität Magdeburg)

Benno Kreuels (2009) : Räumliche Variation der Inzidenz von Plasmodium falciparum Malaria und ihr Einfluss auf die Wirksamkeit einer intermittierenden präventiven Behandlung bei Kleinkindern aus einem Gebiet hoher Endemizität (Universität Hamburg)

Ines Moosmeyer (2009) : Bestimmung der Multiklonalität der asymptomatischen Plasmodium-falciparum-Infektion im holoendemischen Süden Nigerias anhand der Merozoitenoberflächenantigene msp1 und msp2 (Charité - Universitätsmedizin Berlin, Humboldt-Univerität zu Berlin)

Nadine Schreiber (2007) : Entwicklung der Immunantwort gegen variable Oberflächenantigene von Plasmodium falciparum nach intermittierender, präventiver Therapie mit Sulfadoxin-Pyrimethamin bei afrikanischen Kleinkindern (Institut für Pharmazie, Abteilung Pharmazeutische Biologie und Mikrobiologie der Universität Hamburg)

Vera von Kalckreuth (2007) : Humane AE1-Polymorphismen und deren Bedeutung für Manifestationsformen und Fatalität der schweren Malaria tropica von Kindern aus einem hyperendemischen Gebiet in Ghana (Fachbereich Medizin der Universität Hamburg)

Claudia von Reden (2007) : Einfluss sozioökonomischer Parameter auf die Gewichtsentwicklung das Vorkommen von Infektionen mit Plasmodium falciparum bei Säuglingen in Ghana, West-Afrika (Institut für Med. Mikrobiologie und Hygiene der Universität zu Lübeck)

Florian Marks (2006) : Untersuchungen zur Resistenz von Plasmodium falciparum gegen Sulfadoxin/Pyrimethamin bei intermittierender Malariatherapie

Silvia Niesporek (2004) : Assoziationen von TAP1/LMP2-Promotor-Varianten und Allelen des TAP1- und LMP2-Gens mit den Manifestationsformen der Plasmodien-Infektion

Axel Kempa (2003) : Assoziation von HLA-Klasse-II-DQ-Allelen mit der Prävalenz von Plasmodien-Infektionen in Nigeria

Diploma and Master Thesis

Norbert Georg Schwarz (2016) : Analysing clinical studies with composite endpoints: Comparing a weighting strategy by Bakal et al. to a non-weighted approach (Ruprecht-Karls-Universität Heidelberg)

Enusa Ramani (2012) : Economic assessment of diagnostic systems against neglected infectious diseases in African children under five years of age (Hochschule für angewandte Wissenschaften)

Ralf Krumkamp (2010) : Respiratory tract infections in Ashanti province in Ghana – extrapolating disease rates by linking hospital records to a probability model for hospital attendance (Johannes Gutenberg-Universität Mainz)

Christina Kreuzberg (2009) : Risikofaktoren für eine Epstein-Barr-Virus – Infektion bei ghanaischen Kindern (Johannes Gutenberg – Universität Mainz)

Contact

Prof. Dr. Jürgen May

Phone: +49 40 42818-369
Fax: +49 40 42818-512
E-Mail: may@bnitm.de


Secretary

Daniela Krüger (-402)

Scientific Staff

Jörg Blessmann (-243) (Laos/Vietnam)
Denise Dekker (-535)
Daniel Eibach (-504)
Nicole Gilberger (-639)
Benedikt Hogan (-639)
Ralf Krumkamp (-535)
Oumou Maiga Ascofaré (KCCR, Ghana)
Eva Mertens (-244)
Dewi Ismajani Puradiredja (-243)
Nimako Sarpong (KCCR, Ghana)
Norbert Schwarz (-504)
Ricardo Strauss (-243)
Thorsten Thye (-208)

Technical staff

Nassim Behjat (-244)
Doris Winter (-244)

Data Management

Anna Jaeger (-503)
Wibke Loag (-503)

Project Management

Cassandra Aldrich (-268)
Maike Lamshöft (on leave)
Lisa Reigl (-268)

Associated Scientists

Julius Fobil (UGSPH, Accra)
Benno Kreuels (UKE, Hamburg)
Peter Sothmann (UKE, Hamburg)
Christof Vinnemeier (UKE, Hamburg)

Doctoral/PhD Students

Matilda Ayim-Akonor (-243)
Luise Ammer
Lutz Ehlkes
Elina Fechtner
Konstantin Franke
Wiebke Herr
Vinzent Levermann
Kolja Nolte
Christina Rohmann
Bismark Sarfo
Stefanie Steierberg
Marlow Zimmermann (-243)

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