Arbeitgruppe: Iris Bruchhaus, Thorben Matthies, Corinna Lender, Finn Fürstenwerth, Michael Dörpinghaus, Lisa Roth, Susann Ofori, Pedro Lubiana, Torben Rehn (es fehlt: Dirk Lercher)
Iris Bruchhaus, Thorben Matthies, Corinna Lender, Finn Fürstenwerth, Michael Dörpinghaus, Lisa Roth, Susann Ofori, Pedro Lubiana, Torben Rehn (es fehlt: Dirk Lercher)

Overview

PROJECTS

  • Understanding the pathogenicity of Entamoeba histolytica
  • Plasmodium falciparum cytoadhesion - Characterization of receptor-ligand interactions
  • Evolution of malaria parasites and their interaction with their host cell

Research Projects

Understanding the pathogenicity of Entamoeba histolytica

Corinna Lender, Dirk Lercher, Thorben Matthies (former: Martin Meyer)

The pathogen Entamoeba histolytica can live asymptomatically in the human gut, or it can disrupt the intestinal barrier and induce life-threatening abscesses in different organs, most often in the liver. The molecular framework that enables this invasive, highly pathogenic phenotype is still not well understood. In order to identify factors that are positively or negatively correlated for invasion and destruction of the liver, we used a unique tool, E. histolytica clones that differ dramatically in their pathogenicity, while sharing almost identical genetic background. Based on comprehensive transcriptome studies of these clones, we identified a set of candidate genes that are potentially involved in pathogenicity. So far, using ectopic overexpression of the most promising candidates, either in pathogenic or in non-pathogenic Entamoeba clones, we identified genes where high expression reduced pathogenicity and only one gene that increased pathogenicity to a certain extend.

In this project we focus on the further characterization of the candidates by

  • generation and characterization of overexpressing and silencing transfectants
  • biochemical characterization of the identified candidates.
  • Furthermore, to study the host response to an E. histolytica infection mice were infected with pathogenic and non-pathogenic clones, the transcriptomes of the mice liver cells were analysed and compared.

Supported by the DFG and the Jürgen Manchot Stiftung. Collaboration: Hanna Lotter

Characterization of the interaction between Plasmodium infected erythrocytes and human endothelial receptors (receptor-ligand interaction)

Lisa Roth, Susann Ofori (former: Nahla Metwally)

P. falciparum is responsible for most of the morbidity and mortality accompanying malaria infections in humans. According to WHO, 212 million cases were reported globally in 2016. Malaria caused 429 000 malaria deaths (range 235 000–639 000) from which 292 000 deaths were children under 5 years of age, mainly in Sub-Saharan Africa (WHO, World Malaria Report 2016).

A major virulence determinant for this parasite is attributed to the ability of P. falciparum infected erythrocytes (IEs) to evade the immune system and to adhere in small blood vessels of vital organs most likely the small intestine, heart, lung and brain within its human host. This cytoadhesion of IEs is the major reason for the most severe pathological phenotypes observed in malaria, which include blood flow obstruction, hypoxia, induction of inflammatory immune responses, endothelial dysfunction, tissue damage and, ultimately, organ failure.

The interaction of IEs with host endothelial receptors is mainly mediated by members of the PfEMP1 family. The PfEMP1 encoding var genes vary greatly from parasite to parasite, giving rise to an enormous repertoire of different var genes in nature. Var genes are mutually exclusively expressed in ring-stage parasites, meaning that only one PfEMP1 variant is localized on the surface of IEs at a given time. PfEMP1 molecules consist of a single intracellular and transmembrane domain and several different extracellular domains. They can be classified into the groups A, B, B/A, C, B/C and E, or based on the existence of 23 domain cassettes (DCs).

Until now, at least 23 endothelial receptors have been identified to interact with P. falciparum IEs. However, the interactions to only a few of them (CD36, ICAM-1, and EPCR) were studied in detail and the extracellular PfEMP1 domains responsible for adhesion were identified.

In this project we focus on three major aspects.

  • Identification of PfEMP1 ligands/domains mediating cytoadhesion to various receptors
  • Characterization of the receptor-ligand phenotypes using a fluid unit and atomic force microscopy
  • Identification of new endothelial receptors involved in cytoadhesion of infected erythrocytes

Supported by the DAAD, Joachim Herz Stiftung and PIER
Collaborators: Thomas Gutsmann (FZ Borstel), Henning Tidow (Chemie, Universität Hamburg)

Importance of the VIR proteins of Plasmodium vivax for the cytoadhesion of infected erythrocytes and the formation of a chronic infection

Torben Rehn (former: Pedro Lubiana)

The importance of infection with Plasmodium vivax has been underestimated for a long time, although approx. 2.5 billion people are at risk getting infected by P. vivax and this causative agent is responsible for 70-390 million malaria infections annually. In the past decades, infection with P. vivax has been characterized as mild malaria infection, as, in contrast to infection with P. falciparum, P. vivax infection is not fatal in most cases. However, in the last years reports have been accumulated in which severe progressive forms could be attributed to an infection with P. vivax.

Recent studies suggest that the reticulocytes infected by P. vivax are removed from the blood stream and accumulate in different organs such as spleen, bone marrow and lung. However, it is controversially discussed whether classical cytoadhesion to endothelial receptors or non-classical cytoadhesion to non-endothelial structures of the organs occurs. There is the hypothesis that in P. vivax proteins of the approx. 295 members comprising VIR protein family are responsible for the establishment of a chronic infection as well as for cytoadhesion.

  • In this project using in vitro and in vivo studies with the help of P. vivax patient isolates, transgenic parasites, immunofluorescence microscopy and receptor binding studies under flow and static conditions, it should be investigated whether members of the VIR protein family are transported to the erythrocyte membrane and mediate cytoadhesion.

Supported by the DFG and Jürgen Manchot Stiftung. Collaborators: Thorsten Burmester, Tobias Spielmann

Changes in the transcriptome of human brain microvascular endothelial cells due to a Plasmodium falciparum malaria infection

Michael Dörpinghaus, Finn Fürstenwerth

Plasmodium infected erythrocytes can cytoadhere to endothelial cells, which is mainly mediated by proteins of the PfEMP1 family. Depending on the location of the binding, cytoadherence can have severe consequences for the human host. If infected erythrocytes bind to endothelial cells in the brain, cerebral malaria (CM) can occur. Nevertheless, cytoadhesion is necessary but not sufficient to explain the severe outcome. The binding of infected erythrocytes to endothelia cells also induced inflammatory response and endothelial dysfunction, which contributes the severity of a CM.

  • In this project, we focus on the pathogenic mechanisms leading to CM. The transcriptional changes of human brain microvascular endothelial cells (HBMECs) and parasite populations that bind to those cells are at the centre of our interest.

Supported by the Jürgen Manchot Stiftung. Collaborator: Thomas Jacobs

Publication Highlights

Laborgruppe Bruchhaus

Characterisation of Plasmodium falciparum populations selected on the human endothelial receptors P-selectin, E-selectin, CD9 and CD151.
Metwally NG, Tilly AK, Lubiana P, Roth LK, Dörpinghaus M, Lorenzen S, Schuldt K, Witt S, Bachmann A, Tidow H, Gutsmann T, Burmester T, Roeder T, Tannich E, Bruchhaus I.
Sci Rep. 2017 Jun 22;7(1):4069. doi: 10.1038/s41598-017-04241-3.

Overexpression of Differentially Expressed Genes Identified in Non-pathogenic and Pathogenic Entamoeba histolytica Clones Allow Identification of New Pathogenicity Factors Involved in Amoebic Liver Abscess Formation.
Meyer M, Fehling H, Matthiesen J, Lorenzen S, Schuldt K, Bernin H, Zaruba M, Lender C, Ernst T, Ittrich H, Roeder T, Tannich E, Lotter H, Bruchhaus I.
PLoS Pathog. 2016 Aug 30;12(8):e1005853. doi: 10.1371/journal.ppat.1005853.

Phylogeny of haemosporidian blood parasites revealed by a multi-gene approach.
Borner J, Pick C, Thiede J, Kolawole OM, Kingsley MT, Schulze J, Cottontail VM, Wellinghausen N, Schmidt-Chanasit J, Bruchhaus I, Burmester T.
Mol Phylogenet Evol. 2016 Jan;94(Pt A):221-31. doi: 10.1016/j.ympev.2015.09.003. Epub 2015 Sep 11.

Type of in vitro cultivation influences cytoadhesion, knob structure, protein localization and transcriptome profile of Plasmodium falciparum.
Tilly AK, Thiede J, Metwally N, Lubiana P, Bachmann A, Roeder T, Rockliffe N, Lorenzen S, Tannich E, Gutsmann T, Bruchhaus I.
Sci Rep. 2015 Nov 16;5:16766. doi: 10.1038/srep16766.

Evidence of promiscuous endothelial binding by Plasmodium falciparum-infected erythrocytes.
Esser C, Bachmann A, Kuhn D, Schuldt K, Förster B, Thiel M, May J, Koch-Nolte F, Yáñez-Mó M, Sánchez-Madrid F, Schinkel AH, Jalkanen S, Craig AG, Bruchhaus I, Horstmann RD.
Cell Microbiol. 2014 Jan 21. doi: 10.1111/cmi.12270

All Publications

Laborgruppe Bruchhaus
2016

Octopamine controls starvation resistance, life span and metabolic traits in Drosophila.
Li Y, Hoffmann J, Li Y, Stephano F, Bruchhaus I, Fink C, Roeder T.
Sci Rep. 2016 Oct 19;6:35359.

Overexpression of Differentially Expressed Genes Identified in Non-pathogenic and Pathogenic Entamoeba histolytica Clones Allow Identification of New Pathogenicity Factors Involved in Amoebic Liver Abscess Formation.
Meyer M, Fehling H, Matthiesen J, Lorenzen S, Schuldt K, Bernin H, Zaruba M, Lender C, Ernst T, Ittrich H, Roeder T, Tannich E, Lotter H, Bruchhaus I.
PLoS Pathog. 2016 Aug 30;12(8):e1005853. doi: 10.1371/journal.ppat.1005853.

Social stress increases the susceptibility to infection in the ant Harpegnathos saltator
Schneider SA, Scharffetter C, Wagner AE, Boesch C, Bruchhaus I, Rimbach G, Roeder T.
Sci Rep. 2016 May 10;6:25800. doi: 10.1038/srep25800.

IL-23 promotes immunopathology and prevents IL-13-dependent tissue repair associated with Ly6Clo monocytes in Entamoeba histolytica-induced liver damage.
Noll J, Helk E, Fehling H, Bernin H, Marggraff C, Jacobs T, Huber S, Pelczar P, Ernst T, Ittrich H, Otto B, Mittrücker HW, Hölscher C, Tacke F, Bruchhaus I, Tannich E, Lotter H.
J Hepatol. 2016 May;64(5):1147-57. doi: 10.1016/j.jhep.2016.01.013.

Phylogeny of haemosporidian blood parasites revealed by a multi-gene approach.
Borner J, Pick C, Thiede J, Kolawole OM, Kingsley MT, Schulze J, Cottontail VM, Wellinghausen N, Schmidt-Chanasit J, Bruchhaus I, Burmester T.
Mol Phylogenet Evol. 2016 Jan;94(Pt A):221-31. doi: 10.1016/j.ympev.2015.09.003. Epub 2015 Sep 11.

Laborgruppe Bruchhaus
2015

Type of in vitro cultivation influences cytoadhesion, knob structure, protein localization and transcriptome profile of Plasmodium falciparum.
Tilly AK, Thiede J, Metwally N, Lubiana P, Bachmann A, Roeder T, Rockliffe N, Lorenzen S, Tannich E, Gutsmann T, Bruchhaus I.
Sci Rep. 2015 Nov 16;5:16766. doi: 10.1038/srep16766.

A comparative study of the localization and membrane topology of members of the RIFIN, STEVOR and PfMC-2TM protein families in Plasmodium falciparum-infected erythrocytes.
Bachmann A, Scholz JA, Janßen M, Klinkert MQ, Tannich E, Bruchhaus I, Petter M.
Malar J. 2015 Jul 16;14:274.

Magnetic Resonance Imaging of Pathogenic Protozoan Parasite Entamoeba histolytica Labeled With Superparamagnetic Iron Oxide Nanoparticles.
Ernst TM, Fehling H, Bernin H, Zaruba MD, Bruchhaus I, Adam G, Ittrich H, Lotter H.
Invest Radiol. 2015 Oct;50(10):709-18.

The Plasmodium falciparum exportome contains non-canonical PEXEL/HT proteins.
Schulze J, Kwiatkowski M, Borner J, Schlüter H, Bruchhaus I, Burmester T, Spielmann T, Pick C.
Mol Microbiol. 2015 Jul;97(2):301-14.

Cysteine peptidases in pathogenesis.
Bruchhaus I, Matthiesen J.
Amebiasis. Biology and Pathogenesis of Entamoeba. Nozaki, Tomoyoshi, Bhattacharya, Alok (Eds.), Springer, 2015. ISBN 978-4-431-55199-7.

Laborgruppe Bruchhaus
2014

Transcriptional regionalization of the fruit fly's airway epithelium
Faisal MN, Hoffmann J, El-Kholy S, Kallsen K, Wagner C, Bruchhaus I, Fink C, Roeder T.
PLoS One. 2014 Jul 14;9(7):e102534.

Evidence of promiscuous endothelial binding by Plasmodium falciparum-infected erythrocytes
Esser C, Bachmann A, Kuhn D, Schuldt K, Förster B, Thiel M, May J, Koch-Nolte F, Yáñez-Mó M, Sánchez-Madrid F, Schinkel AH, Jalkanen S, Craig AG, Bruchhaus I, Horstmann RD.
Cell Microbiol. 2014 May;16(5):701-8.

The cell surface proteome of Entamoeba histolytica
Biller L, Matthiesen J, Kühne V, Lotter H, Handal G, Nozaki T, Saito-Nakano Y, Schümann M, Roeder T, Tannich E, Krause E, Bruchhaus I.
Mol Cell Proteomics. 2014 Jan;13(1):132-44.

Lophophorata monophyletic – after all.
Nesnidal MP, Helmkampf M, Bruchhaus I, Ebersberg I, Hausdorf B.
Deep Metazoan Phylogeny: Backbone of the Tree of Life. 2014. Edited by Waegele JW, Bartholomaeus T, Misof B, Vogt L. Berlin: Walter de Gruyter.

Laborgruppe Bruchhaus
2013

New phylogenomic data support the monophyly of Lophophorata and an Ectoproct-Phoronid clade and indicate that Polyzoa and Kryptrochozoa are caused by systematic bias.
Nesnidal MP, Helmkampf M, Meyer A, Witek A, Bruchhaus I, Ebersberger I, Hankeln T, Lieb B, Struck TH, Hausdorf B.
BMC Evol Biol. 2013 Nov 17;13:253. doi: 10.1186/1471-2148-13-253.

Agent of whirling disease meets orphan worm: phylogenomic analyses firmly place Myxozoa in Cnidaria.
Nesnidal MP, Helmkampf M, Bruchhaus I, El-Matbouli M, Hausdorf B.
PLoS One. 2013;8(1):e54576. doi: 10.1371/journal.pone.0054576. Epub 2013 Jan 30.

Overexpression of specific cysteine peptidases confers pathogenicity to a nonpathogenic Entamoeba histolytica clone.
Matthiesen J, Bär AK, Bartels AK, Marien D, Ofori S, Biller L, Tannich E, Lotter H, Bruchhaus I.
MBio. 2013 Mar 26;4(2).

Laborgruppe Bruchhaus
2012

Genomic diversity of the human intestinal parasite Entamoeba histolytica.
Weedall GD, Clark CG, Koldkjaer P, Kay S, Bruchhaus I, Tannich E, Paterson S, Hall N.
Genome Biol. 2012 May 25;13(5):R38. doi: 10.1186/gb-2012-13-5-r38.

The cysteine protease inhibitors EhICP1 and EhICP2 perform different tasks in the regulation of endogenous protease activity in trophozoites of Entamoeba histolytica.
Šaric M, Irmer H, Eckert D, Bär AK, Bruchhaus I, Scholze H.
Protist. 2012 Jan;163(1):116-28. doi: 10.1016/j.protis.2011.01.003.

Temporal expression and localization patterns of variant surface antigens in clinical Plasmodium falciparum isolates during erythrocyte schizogony.
Bachmann A, Petter M, Tilly AK, Biller L, Uliczka KA, Duffy MF, Tannich E, Bruchhaus I.
PLoS One. 2012;7(11):e49540.

Entamoeba cysteine endopeptidases belonging to the C1 papain superfamily.
Scholze H, Tannich E, Bruchhaus I.
Handbook of Proteolytic Enzymes, 3nd edition, 2012. Rawlings N.D. and Salvesen, G., eds., Ch. 438, Elsevier, pp. 1915-1918.

Laborgruppe Bruchhaus
2011

Phylogenomic analyses of malaria parasites and evolution of their exported proteins.
Pick C, Ebersberger I, Spielmann T, Bruchhaus I, Burmester T.
BMC Evol Biol. 2011 Jun 15;11:167. doi: 10.1186/1471-2148-11-167.

The complete mitochondrial genome of Flustra foliacea (Ectoprocta, Cheilostomata) - compositional bias affects phylogenetic analyses of lophotrochozoan relationships.
Nesnidal MP, Helmkampf M, Bruchhaus I, Hausdorf B.
BMC Genomics. 2011 Nov 23;12:572. doi: 10.1186/1471-2164-12-572.

Highly co-ordinated var gene expression and switching in clinical Plasmodium falciparum isolates from non-immune malaria patients.
Bachmann A, Predehl S, May J, Harder S, Burchard GD, Gilberger TW, Tannich E, Bruchhaus I.
Cell Microbiol. 2011 Sep;13(9):1397-409.

Laborgruppe Bruchhaus
2010

Compositional heterogeneity and phylogenomic inference of metazoan relationships.
Nesnidal MP, Helmkampf M, Bruchhaus I, Hausdorf B.
Mol Biol Evol. 2010 Sep;27(9):2095-104. doi: 10.1093/molbev/msq097.

The essentials of protein import in the degenerate mitochondrion of Entamoeba histolytica.
Dolezal P, Dagley MJ, Kono M, Wolynec P, Liki? VA, Foo JH, Sedinová M, Tachezy J, Bachmann A, Bruchhaus I, Lithgow T.
PLoS Pathog. 2010 Mar 19;6(3):e1000812. doi: 10.1371/journal.ppat.1000812.

Characterization of a subunit of the outer dynein arm docking complex necessary for correct flagellar assembly in Leishmania donovani.
Harder S, Thiel M, Clos J, Bruchhaus I.
PLoS Negl Trop Dis. 2010 Jan 26;4(1):e586. doi: 10.1371/journal.pntd.0000586.

Differences in the transcriptome signatures of two genetically related Entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties.
Biller L, Davis PH, Tillack M, Matthiesen J, Lotter H, Stanley SL Jr, Tannich E, Bruchhaus I.
BMC Genomics. 2010 Jan 26;11:63. doi: 10.1186/1471-2164-11-63.

Phylogenetic relationships within the lophophorate lineages (Ectoprocta, Brachiopoda and Phoronida).
Hausdorf B, Helmkampf M, Nesnidal MP, Bruchhaus I.
Mol Phylogenet Evol. 2010 Jun;55(3):1121-7. doi: 10.1016/j.ympev.2009.12.022.

tRNA gene sequences are required for transcriptional silencing in Entamoeba histolytica.
Irmer H, Hennings I, Bruchhaus I, Tannich E.
Eukaryot Cell. 2010 Feb;9(2):306-14. doi: 10.1128/EC.00248-09.

Caenopores are antimicrobial peptides in the nematode Caenorhabditis elegans instrumental in nutrition and immunity.
Roeder T, Stanisak M, Gelhaus C, Bruchhaus I, Grötzinger J, Leippe M.
Dev Comp Immunol. 2010 Feb;34(2):203-9. doi: 10.1016/j.dci.2009.09.010.

Laborgruppe Bruchhaus
2009

Absence of erythrocyte sequestration and lack of multicopy gene family expression in Plasmodium falciparum from a splenectomized malaria patient.
Bachmann A, Esser C, Petter M, Predehl S, von Kalckreuth V, Schmiedel S, Bruchhaus I, Tannich E.
PLoS One. 2009 Oct 14;4(10):e7459. doi: 10.1371/journal.pone.0007459.

Comparison of two genetically related Entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties.
Biller L, Schmidt H, Krause E, Gelhaus C, Matthiesen J, Handal G, Lotter H, Janssen O, Tannich E, Bruchhaus I.
Proteomics. 2009 Sep;9(17):4107-20. doi: 10.1002/pmic.200900022.

Major cysteine peptidases of Entamoeba histolytica are required for aggregation and digestion of erythrocytes but are dispensable for phagocytosis and cytopathogenicity.
Irmer H, Tillack M, Biller L, Handal G, Leippe M, Roeder T, Tannich E, Bruchhaus I.
Mol Microbiol. 2009 May;72(3):658-67. doi: 10.1111/j.1365-2958.2009.06672.x.

Laborgruppe Bruchhaus
2008

An Entamoeba cysteine peptidase specifically expressed during encystation.
Ebert F, Bachmann A, Nakada-Tsukui K, Hennings I, Drescher B, Nozaki T, Tannich E, Bruchhaus I.
Parasitol Int. 2008 Dec;57(4):521-4. doi: 10.1016/j.parint.2008.07.002.

Phylogenomic analyses of lophophorates (brachiopods, phoronids and bryozoans) confirm the Lophotrochozoa concept.
Helmkampf M, Bruchhaus I, Hausdorf B.
Proc Biol Sci. 2008 Aug 22;275(1645):1927-33. doi: 10.1098/rspb.2008.0372.

Involvement of a Leishmania thymidine kinase in flagellum formation, promastigote shape and growth as well as virulence.
Thiel M, Harder S, Wiese M, Kroemer M, Bruchhaus I.
Mol Biochem Parasitol. 2008 Apr;158(2):152-62. doi: 10.1016/j.molbiopara.2007.12.005.

Characterization of a conserved rhoptry-associated leucine zipper-like protein in the malaria parasite Plasmodium falciparum.
Haase S, Cabrera A, Langer C, Treeck M, Struck N, Herrmann S, Jansen PW, Bruchhaus I, Bachmann A, Dias S, Cowman AF, Stunnenberg HG, Spielmann T, Gilberger TW.
Infect Immun. 2008 Mar;76(3):879-87. doi: 10.1128/IAI.00144-07.

Multigene analysis of lophophorate and chaetognath phylogenetic relationships.
Helmkampf M, Bruchhaus I, Hausdorf B.
Mol Phylogenet Evol. 2008 Jan;46(1):206-14.

Laborgruppe Bruchhaus
2007

Structure and content of the Entamoeba histolytica genome.
Clark CG, Alsmark UC, Tazreiter M, Saito-Nakano Y, Ali V, Marion S, Weber C, Mukherjee C, Bruchhaus I, Tannich E, Leippe M, Sicheritz-Ponten T, Foster PG, Samuelson J, Noël CJ, Hirt RP, Embley TM, Gilchrist CA, Mann BJ, Singh U, Ackers JP, Bhattacharya S, Bhattacharya A, Lohia A, Guillén N, Duchêne M, Nozaki T, Hall N.
Adv Parasitol. 2007;65:51-190.

Spiralian phylogenomics supports the resurrection of Bryozoa comprising Ectoprocta and Entoprocta.
Hausdorf B, Helmkampf M, Meyer A, Witek A, Herlyn H, Bruchhaus I, Hankeln T, Struck TH, Lieb B.
Mol Biol Evol. 2007 Dec;24(12):2723-9.

Protozoan parasites: programmed cell death as a mechanism of parasitism.
Bruchhaus I, Roeder T, Rennenberg A, Heussler VT.
Trends Parasitol. 2007 Aug;23(8):376-83.

The Entamoeba histolytica genome: primary structure and expression of proteolytic enzymes.
Tillack M, Biller L, Irmer H, Freitas M, Gomes MA, Tannich E, Bruchhaus I.
BMC Genomics. 2007 Jun 14;8:170.

Proteomic analysis of Entamoeba histolytica.
Tolstrup J, Krause E, Tannich E, Bruchhaus I.
Parasitology. 2007 Feb;134(Pt 2):289-98.

Laborgruppe Bruchhaus
2006

The second cysteine protease inhibitor, EhICP2, has a different localization in trophozoites of Entamoeba histolytica than EhICP1.
Saric M, Vahrmann A, Bruchhaus I, Bakker-Grunwald T, Scholze H.
Parasitol Res. 2006 Dec;100(1):171-4.

Increased expression of the major cysteine proteinases by stable episomal transfection underlines the important role of EhCP5 for the pathogenicity of Entamoeba histolytica.
Tillack M, Nowak N, Lotter H, Bracha R, Mirelman D, Tannich E, Bruchhaus I.
Mol Biochem Parasitol. 2006 Sep;149(1):58-64.

Expression of a mitochondrial peroxiredoxin prevents programmed cell death in Leishmania donovani.
Harder S, Bente M, Isermann K, Bruchhaus I.
Eukaryot Cell. 2006 May;5(5):861-70.

Laborgruppe Bruchhaus
2005

In vitro amoebicidal activity of some medicinal plants of the bambun region (Cameroon)
Moundipa PF, Melanie Flore KG, Bilong Bilong CF, Bruchhaus, I.
Afr. J. Trad. CAM 2005, 2, 113-121.

The genome of the protist parasite Entamoeba histolytica.
Loftus B, Anderson I, Davies R, Alsmark UC, Samuelson J, Amedeo P, Roncaglia P, Berriman M, Hirt RP, Mann BJ, Nozaki T, Suh B, Pop M, Duchene M, Ackers J, Tannich E, Leippe M, Hofer M, Bruchhaus I, Willhoeft U, Bhattacharya A, Chillingworth T, Churcher C, Hance Z, Harris B, Harris D, Jagels K, Moule S, Mungall K, Ormond D, Squares R, Whitehead S, Quail MA, Rabbinowitsch E, Norbertczak H, Price C, Wang Z, Guillén N, Gilchrist C, Stroup SE, Bhattacharya S, Lohia A, Foster PG, Sicheritz-Ponten T, Weber C, Singh U, Mukherjee C, El-Sayed NM, Petri WA Jr, Clark CG, Embley TM, Barrell B, Fraser CM, Hall N.
Nature. 2005 Feb 24;433(7028):865-8.

Identification of EhICP1, a chagasin-like cysteine protease inhibitor of Entamoeba histolytica.
Riekenberg S, Witjes B, Sari? M, Bruchhaus I, Scholze H.
FEBS Lett. 2005 Mar 14;579(7):1573-8.

Laborgruppe Bruchhaus
2004

Resistance of Entamoeba histolytica to the cysteine proteinase inhibitor E64 is associated with secretion of pro-enzymes and reduced pathogenicity.
Nowak N, Lotter H, Tannich E, Bruchhaus I.
J Biol Chem. 2004 Sep 10;279(37):38260-6.

A peroxiredoxin specifically expressed in two types of pharyngeal neurons is required for normal growth and egg production in Caenorhabditis elegans.
Isermann K, Liebau E, Roeder T, Bruchhaus I.
J Mol Biol. 2004 May 7;338(4):745-55.

Entamoeba histolytica trophozoites transfer lipophosphopeptidoglycans to enteric cell layers.
Lauwaet T, Oliveira MJ, De Bruyne G, Bruchhaus I, Duchêne M, Mareel M, Leroy A.
Int J Parasitol. 2004 Apr;34(5):549-56.

Effects of bisphosphonates on the growth of Entamoeba histolytica and Plasmodium species in vitro and in vivo.
Ghosh S, Chan JM, Lea CR, Meints GA, Lewis JC, Tovian ZS, Flessner RM, Loftus TC, Bruchhaus I, Kendrick H, Croft SL, Kemp RG, Kobayashi S, Nozaki T, Oldfield E.
J Med Chem. 2004 Jan 1;47(1):175-87.

Laborgruppe Bruchhaus
2003

Developmentally induced changes of the proteome in the protozoan parasite Leishmania donovani.
Bente M, Harder S, Wiesgigl M, Heukeshoven J, Gelhaus C, Krause E, Clos J, Bruchhaus I.
Proteomics. 2003 Sep;3(9):1811-29.

The intestinal protozoan parasite Entamoeba histolytica contains 20 cysteine protease genes, of which only a small subset is expressed during in vitro cultivation.
Bruchhaus I, Loftus BJ, Hall N, Tannich E.
Eukaryot Cell. 2003 Jun;2(3):501-9.

Laborgruppe Bruchhaus
2002

Differential gene expression in Entamoeba histolytica isolated from amoebic liver abscess.
Bruchhaus I, Roeder T, Lotter H, Schwerdtfeger M, Tannich E.
Mol Microbiol. 2002 May;44(4):1063-72.

Recombinant expression and purification of an enzymatically active cysteine proteinase of the protozoan parasite Entamoeba histolytica.
Hellberg A, Nowak N, Leippe M, Tannich E, Bruchhaus I.
Protein Expr Purif. 2002 Feb;24(1):131-7.

Laborgruppe Bruchhaus
2001

Comparative proteome analysis of Leishmania donovani at different stages of transformation from promastigotes to amastigotes.
Thiel M, Bruchhaus I.
Med Microbiol Immunol. 2001 Nov;190(1-2):33-6.

Introns of Entamoeba histolytica and Entamoeba dispar.
Willhoeft U, Campos-Góngora E, Touzni S, Bruchhaus I, Tannich E.
Protist. 2001 Jul;152(2):149-56.

Overexpression of cysteine proteinase 2 in Entamoeba histolytica or Entamoeba dispar increases amoeba-induced monolayer destruction in vitro but does not augment amoebic liver abscess formation in gerbils.
Hellberg A, Nickel R, Lotter H, Tannich E, Bruchhaus I.
Cell Microbiol. 2001 Jan;3(1):13-20.

Laborgruppe Bruchhaus
2000

A simple and reliable 5'-RACE approach.
Schramm G, Bruchhaus I, Roeder T.
Nucleic Acids Res. 2000 Nov 15;28(22):E96.

Superoxide dismutase reduces susceptibility to metronidazole of the pathogenic protozoan Entamoeba histolytica under microaerophilic but not under anaerobic conditions.
Wassmann C, Bruchhaus I.
Arch Biochem Biophys. 2000 Apr 1;376(1):236-8.

Two major 'higher molecular mass proteinases' of Entamoeba histolytica are identified as cysteine proteinases 1 and 2.
Hellberg A, Leippe M, Bruchhaus I.
Mol Biochem Parasitol. 2000 Feb 5;105(2):305-9.

Laborgruppe Bruchhaus
1998

Isolation of ultrapure supercoiled plasmid-DNA using preparative electrophoresis.
Roeder T, Görich D, Roeder NP, Bruchhaus I.
Electrophoresis. 1998 Jul;19(10):1575-6.

Recombinant expression and biochemical characterization of an NADPH:flavin oxidoreductase from Entamoeba histolytica.
Bruchhaus I, Richter S, Tannich E.
Biochem J. 1998 Mar 15;330 ( Pt 3):1217-21.

Isolation and molecular characterization of a surface-bound proteinase of Entamoeba histolytica.
Jacobs T, Bruchhaus I, Dandekar T, Tannich E, Leippe M.
Mol Microbiol. 1998 Jan;27(2):269-76.

Laborgruppe Bruchhaus
1997

Removal of hydrogen peroxide by the 29 kDa protein of Entamoeba histolytica.
Bruchhaus I, Richter S, Tannich E.
Biochem J. 1997 Sep 15;326 ( Pt 3):785-9.

Characterization of two E. histolytica proteins that inactivate reactive oxygen species.
Bruchhaus I, Richter S, Tannich E.
Arch Med Res. 1997;28 Spec No:91-2.

Problematik der Isolierung genomischer DNA.
Bruchhaus I, Müller H-J
BIOspektrum, 1997, 1, 68 + 73.

Laborgruppe Bruchhaus
1995

Free radicals and protozoa.
Bruchhaus I, Dive D.
Protistol Actual. 1995, 195-203.

Identification of an Entamoeba histolytica gene encoding a protein homologous to prokaryotic disulphide oxidoreductases.
Bruchhaus I, Tannich E.
Mol Biochem Parasitol. 1995 Mar;70(1-2):187-91.

Laborgruppe Bruchhaus
1993

Unusual gene organization in the protozoan parasite Entamoeba histolytica.
Bruchhaus I, Leippe M, Lioutas C, Tannich E.
DNA Cell Biol. 1993 Dec;12(10):925-33.

Primary structure of the pyruvate phosphate dikinase in Entamoeba histolytica.
Bruchhaus I, Tannich E.
Mol Biochem Parasitol. 1993 Nov;62(1):153-6.

Analysis of the genomic sequence encoding the 29-kDa cysteine-rich protein of Entamoeba histolytica.
Bruchhaus I, Tannich E.
Trop Med Parasitol. 1993 Jun;44(2):116-8.

Laborgruppe Bruchhaus
1992

Purification of glutathione reductase from muscle of the adult parasitic nematode Ascaris suum.
Komuniecki R, Bruchhaus I, Ilg T, Wilson K, Zhang Y, Fairlamb AH.
Mol Biochem Parasitol. 1992 Apr;51(2):331-3.

Recombinant expression, purification and biochemical characterization of a superoxide dismutase from Entamoeba histolytica.
Bruchhaus I, Brattig NW, Tannich E.
Arch Med Res. 1992;23(2):27-9.

Laborgruppe Bruchhaus
1991

Pathogenic and nonpathogenic Entamoeba histolytica: identification and molecular cloning of an iron-containing superoxide dismutase.
Tannich E, Bruchhaus I, Walter RD, Horstmann RD.
Mol Biochem Parasitol. 1991 Nov;49(1):61-71.

News from the group

Muitos parabens senhor doutor! (07.04.2017)

Finally - it - is - done. CONGRATULATIONS Dr. Martin Meyer!!!!! (13.10.2016)

Congratulations Dr. rer. nat. Nahla Metwally

 

 

 

 

Alumni

Doctoral Thesis

Dr. rer. nat. Pedro Lubiana (2014-2017) : Charakterisierung der Interaktion zwischen Plasmodium infizierten Erythrozyten und humanen Endothelrezeptoren.

Dr. rer. nat. Martin Meyer (2013-2016) : Identifizierung neuer Pathogenitätsfaktoren durch Überexpression differentiell exprimierter Gene in pathogenen und nicht-pathogenen Entamoeba histolytica (SCHAUDINN, 1903) Klonen.

Dr. rer. nat. Nahla Metwally (2013-2016) : Analyses of the transcriptome profiles of Plasmodium falciparum infected erythrocytes selected for binding to the human endothelial receptors (ICAM-1, P-selectin, E-selectin, CD9 and CD151).

Dr. rer. nat. Helena Fehling (2012-2016) : Analyse der Unterschiede pathogener und apathogener Entamoeba histolytica (SCHAUDINN, 1903) Klone in der Parasit-Wirt Interaktion.

Dr. rer. nat. Ann-Kathrin Tilly (2012-2015) : Adhäsion von Plasmodium falciparum (Welch, 1897) infizierten Erythrozyten an den humanen Endothelrezeptor P-Selektin.

Dr. rer. nat. Maximilian Nesnidal (2009-2013) : Molekularphylogenetische Analyse basaler Linien der Bilateria.

Dr. rer. nat. Jenny Matthiesen (2009-2012) : Charakterisierung des pathogenen Phänotyps von Entamoeba histolytica (Schaudinn, 1903).

Dr. rer. nat. Ghassan Handal (2007-2010) : Regulation of antioxidant enzymes in Entamoeba histolytica (Schaudinn, 1903).

Dr. rer. nat. Anna Bachmann (2007-2010) : Genexpression und Lokalisation variabler Oberflächenproteine des Malariaerregers Plasmodium falciparum (Welch, 1897).

Dr. rer. nat. Sabine Predehl (2007-2010) : Funktionsanalyen der PfEMP1 Proteinfamilie von Plasmodium falciparum (Welch, 1897).

Dr. rer. nat. Laura Biller (2006-2009) : Identifizierung der Pathogenitätsfaktoren von Entamoeba histolytica mittels Phänotypisierung und vergleichender Expressionsanalysen.

Dr. rer. nat. Martin Helmkampf (2005-2009) : Molecular phylogenetic analysis of Lophophorata and Chaetognatha.

Dr. rer. nat. Manuela Tillack (2004-2008) : Die Bedeutung der Cystein-Proteinasen des parasitischen Protozoons Entamoeba histolytica für die Phagozytose, Gewebszerstörung, Invasion und Abszessbildung.

Dr. rer. nat. Meike Thiel (2000-2006) : Identifizierung und Charakterisierung stadienspezifischer Proteine von Leishmania donovani.

Dr. rer. nat. Simone Harder (2001-2005) : Proteomanalyse der Stadiendifferenzierung von Leishmania donovani.

Dr. rer. nat. Kerstin Isermann (2001-2004) : Charakterisierung der Peroxiredoxine von Caenorhabditis elegans.

Dr. rer. nat. Nicolas Nowak (2000-2004) : Charakterisierung der Peptidasen von Entamoeba histolytica.

Dr. rer. nat. Claudia Waßmann (1997-2000) : Charakterisierung enzymatischer Antioxidantien von Entamoeba histolytica.

Dr. rer. nat. Andrea Hellberg (1997-2000) : Cystein-Proteinasen von Entamoeba histolytica und Entamoeba dispar.

Dr. med. Symi Richter (1996) : Rekombinante Expression und funktionelle Charakterisierung eines Peroxiredoxins und einer NADPH:Flavin-Oxidoreduktase des Protozoons Entamoeba histolytica.

Diploma and Master Thesis

Sarah Steinfurth (2016) : Studien zur Identifizierung der Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) Bindungsdomänen für die Endothelrezeptoren CD9 und P-Selektin

Marius Schmitt (2016) : Localization of Plasmodium vivax (Grassi and Feletti, 1890) VIR proteins by means of Plasmodium falciparum (Welch, 1897) transgenic cell lines.

Eugenia Reit (2015) : Production of transgenic CHO–cell lines for investigation of interaction of Plasmodium falciparum with the human endothelial receptors VCAM–1, PECAM–1 and VE–cadherin.

Michael Dörpinghaus (2015) : Development of a system to characterize the binding properties of Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) domains to human endothelial receptors

Lisa Roth (2015) : Characterization of binding characteristics of human endothelial receptors among human erythrocytes infected with malaria parasite Plasmodium falciparum.

Ellen Drews (2014) : Etablierung lentiviraler Expressionssysteme zur Charakterisierung der Interaktion zwischen Plasmodium falciparum (Welch, 1897) infizierten Erythrozyten und humanen Endothelrezeptoren.

Jenny Thiede (2013) : Lokalisierung ausgewählter Proteine mit alternativen, putativen PEXEL-Motiv des Malariaerregers Plasmodium falciparum (Welch, 1897).

Anne Bartels (2013) : Die Überexpression von Cysteinpeptidasen beeinflusst die Virulenz nicht-pathogener Entamoeba histolytica [SCHAUDINN, 1903] Trophozoiten.

Vera Kühne (2012) : Charakterisierung des putativen Membranproteoms von Entamoeba histolytica (Schaudinn, 1903).

Judith Scholz (2012) : Genexpression und Lokalisation variabler Oberflächenproteine in Merozoiten des Malariaerregers Plasmodium falciparum (Welch, 1897).

Olga Shpigelman (2011) : Identification of binding domains of PfEMP1 molecules to the receptor ICAM–1 of Plasmodium falciparum (Welch, 1897).

Ann-Kathrin Tilly (2011) : Expression und Lokalisation variabler Oberflächenantigene des Malariaerregers Plasmodium falciparum (Welch, 1897) während der erythrozytären Schizogonie.

Ann-Kathrein Bär (2010) : Expressionsprofil der Cysteinpeptidasen von Entamoeba histolytica (SCHAUDINN 1903) während der Leberabszessbildung im experimentellen Mausmodell (Mus musculus, LINNAEUS 1758).

Johanna Anige (2010) : Charakterisierung der Bindungseigenschaften ausgewählter Domänen der PfEMP1-Moleküle von Plasmodium falciparum (Welch, 1897) mit Hilfe der Biacore-Technologie.

Steffi Renk (2010) : Adhärenz Plasmodium falciparum (Welch, 1897) infizierter Erythrozyten an Endothelrezeptoren: Einfluss der in vitro-Kultivierung.

Babette Drescher (2009) : Charakterisierung und Lokalisierung ausgewählter Mitglieder der aig-Genfamilie von Entamoeba histolytica (Schaudinn, 1903).

Jenny Matthiesen (2008) : Charakterisierung putativer EhAIG-Proteine und Aufbau eines Protein-Markierungssystems bei Entamoeba histolytica (Schaudinn, 1903).

Dennis Marien (2007) : Expression der Peptidasen und weiterer putativer Pathogenitätsfaktoren von Entamoeba histolytica während der Leberabszessbildung.

Nina Alex (2006) : Expressionsstudien von CudA-homologen (culmination defective) Genen in Entamoeba histolytica (Schaudinn, 1903) und Entamoeba invadens (Rodhain, 1934).

Maya Kono (2007) : Charakterisierung putativer mitosomaler Proteine in Entamoeba histolytica.

Sabine Predehl (2007) : Dynamik der var-Genexpression bei Plasmodium falciparum.

Anna Bachmann (2006) : Analyse der var-Genexpression bei Plasmodium falciparum.

Mona Shahhossini (2004) : Regulation der Cystein-Proteinasen von Entamoeba invadens während der Enzystierung.

Jörn Tolstrup (2004) : Proteomanalyse Cystein-Proteinase überexprimierender Entamoeba histolytica.

Verena Diehm (2001) : Analyse der Expressionsmuster der Proteasegene des parasitischen Protozoons Entamoeba histolytica.

Simone Harder (2000) : Untersuchungen zur Inhibition der Proteinsynthese durch "Antisense"-RNA und RNA"-Interference" bei Entamoeba histolytica.

Meike Thiel (1999) : Identifizierung stadienspezifisch exprimierter Proteine des parasitischen Protozoons Leishmania donovani.

Bachelor Thesis

Elena Baron (2015) : Localization of Plasmodium vivax (Grassi and Feletti, 1890) VIR proteins by means of Plasmodium falciparum (Welch, 1897) transgenic cell lines.

Postdocs

Dr. rer. nat. Jenny Matthiesen (2012-2016) : Pathogenicity factors and cysteine peptidases of Entamoeba histolytica