Arbeitgruppe: Iris Bruchhaus, Thorben Matthies, Corinna Lender, Finn Fürstenwerth, Michael Dörpinghaus, Lisa Roth, Susann Ofori, Pedro Lubiana, Torben Rehn (es fehlt: Dirk Lercher)
Iris Bruchhaus, Thorben Matthies, Corinna Lender, Finn Fürstenwerth, Michael Dörpinghaus, Lisa Roth, Susann Ofori, Pedro Lubiana, Torben Rehn (es fehlt: Dirk Lercher)

Overview

PROJECTS

  • Understanding the pathogenicity of Entamoeba histolytica
  • Plasmodium falciparum cytoadhesion - Characterization of receptor-ligand interactions
  • Evolution of malaria parasites and their interaction with their host cell

Research Projects

Understanding the pathogenicity of Entamoeba histolytica

Martin Meyer, Corinna Lender, Dirk Lercher

The infection with Entamoeba histolytica can lead to invasive diseases such as ulcerative colitis or extraintestinal abscesses. Since all proposed pathogenicity factors are present in both, pathogenic and non-pathogenic amoeba, the mechanism of virulence is still unclear. We recently identified two syngenic cell lines derived from the laboratory strain HM-1:IMSS, which consistently differ in virulence. Cell line A is incapable to induce abscesses, whereas cell line B provokes large abscesses. Transcriptomic and proteomic studies revealed various differences, suggesting that the abundance of particular proteins is responsible for amoeba pathogenicity. However, generation of clones derived from the two cell lines indicated that in particular cell line B is more divergent then previously expected, as single clones showed different degrees in virulence ranging from avirulent to highly virulent phenotypes. Despite these phenotypic variations, preliminary sequence analyses revealed only little sequence differences between the clones. Consequently, we have now a robust tool that will help to understand the origin of amoebic pathogenesis. Therefore, a selection of non-pathogenic and pathogenic clones should be further characterised i) to identify pathogenicity factors of E. histolytica based on the comparison between these clones, ii) to elucidate the transcriptional dynamics of these clones during abscess formation, and iii) to understand the interaction of amoebae with host cells during the process of abscess formation. New technical achievements such as mRNA sequencing of abscess-derived amoebae, small animal-magnet resonance imaging, advanced immunohistochemistry, and intravital microscopy will be employed. Merging the findings obtained by these investigations will be useful to unravel the molecular mechanism of amoebic pathogenesis.

Supported by the DFG and the Jürgen Manchot Stiftung.
PIs: Hannelore Lotter, Iris Bruchhaus

Plasmodium falciparum cytoadhesion - Characterization of receptor-ligand interactions

Nahla Metwally, Lisa Roth, Michael Dörpinghaus, Susann Ofori

The human malaria parasite Plasmodium falciparum is able to avoid spleen-dependent killing by adhesion of infected erythrocytes to human endothelial receptors that line microvascular venules. This sequestration of infected red blood cells (iRBC) is the major reason for the most severe pathological phenotypes observed in malaria, including obstruction of blood flow, hypoxia, tissue damage and ultimately, organ failure. The specific binding is caused by parasite-derived proteins located on the erythrocyte membrane. Currently, only one protein family of the parasite is known to be involved in these receptor-ligand interactions, the members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. The PfEMP1 family is encoded by about 60 var genes per parasite genome and the var gene sequences vary greatly from parasite to parasite, giving rise to an enormous repertoire of different var genes in nature. The various PfEMP1 molecules display different binding characteristics, which is thought to be important for the different clinical outcomes. To date, at least 22 endothelial receptors or molecules are described to be involved in the interaction with iRBC (e.g. CD36, ICAM-1, P-selectin, E-selectin). Nevertheless, for most of the receptors that were described to bind the parasite ligand is not much known. Although the phenomenon that iRBC are sequestered to host epithelial structures during an infection is central for the understanding of malaria pathogenesis, our current knowledge regarding the underlying molecular framework is far from being complete. Here we like to characterise the interaction of iRBC with a selected set of endothelial receptor. The interaction should be analysed under static as well as under flow conditions. Furthermore, of the nature of the bond responsible for binding of iRBC to a specific receptor should be elucidated (catch versus ordinary slip bond). Most importantly, the parasite's ligand responsible for a specific binding should be identified. In addition the protein domain responsible for binding should be mapped, the interaction should be characterized, and the three-dimensional structures should be determined. These results will help us to develop intervention strategies to specifically inhibit binding of those P. falciparum-infected erythrocytes that are responsible for severe clinical courses of malaria.

Supported by the DAAD, Joachim Herz Stiftung and PIER, Jürgen Manchot Stiftung
Collaborators: Thomas Gutsmann (FZ Borstel), Henning Tidow (Chemie, Universität Hamburg)

Evolution of malaria parasites and their interaction with their host cell

Pedro Lubiana and Sonja Obersteller

Fighting malaria requires a deeper understanding of the evolution of the interactions of the Plasmodium parasites with their host cells. Thus, it is of great importance for malaria research (i) to obtain a reliable phylogenetic framework that includes the genus Plasmodium and closely-related malaria parasites, (ii) to uncover pathogenicity-related mechanisms common to all Haemosporida, and (iii) to identify the molecular determinants that underlay the exceptional high pathogenicity of P. falciparum. These studies will provide better insights into the evolution of infection mechanisms of haemosporidian blood parasites and, in particular, those causing human malaria.

Supported by the DFG.
PIs: Thorsten Burmester, Christian Pick (Zoological Institute, University Hamburg), Tobias Spielmann (BNITM), Iris Bruchhaus; PhD Students: Janus Borner, Pedro Lubiana

MOLECULAR PHYLOGENETICS AND EVOLUTION, 94 (2016) 221-231. doi:10.1016/j.ympev.2015.09.003

Publication Highlights

Laborgruppe Bruchhaus

Overexpression of Differentially Expressed Genes Identified in Non-pathogenic and Pathogenic Entamoeba histolytica Clones Allow Identification of New Pathogenicity Factors Involved in Amoebic Liver Abscess Formation.
Meyer M, Fehling H, Matthiesen J, Lorenzen S, Schuldt K, Bernin H, Zaruba M, Lender C, Ernst T, Ittrich H, Roeder T, Tannich E, Lotter H, Bruchhaus I.
PLoS Pathog. 2016 Aug 30;12(8):e1005853. doi: 10.1371/journal.ppat.1005853.

Phylogeny of haemosporidian blood parasites revealed by a multi-gene approach.
Borner J, Pick C, Thiede J, Kolawole OM, Kingsley MT, Schulze J, Cottontail VM, Wellinghausen N, Schmidt-Chanasit J, Bruchhaus I, Burmester T.
Mol Phylogenet Evol. 2016 Jan;94(Pt A):221-31. doi: 10.1016/j.ympev.2015.09.003. Epub 2015 Sep 11.

Type of in vitro cultivation influences cytoadhesion, knob structure, protein localization and transcriptome profile of Plasmodium falciparum.
Tilly AK, Thiede J, Metwally N, Lubiana P, Bachmann A, Roeder T, Rockliffe N, Lorenzen S, Tannich E, Gutsmann T, Bruchhaus I.
Sci Rep. 2015 Nov 16;5:16766. doi: 10.1038/srep16766.

Evidence of promiscuous endothelial binding by Plasmodium falciparum-infected erythrocytes.
Esser C, Bachmann A, Kuhn D, Schuldt K, Förster B, Thiel M, May J, Koch-Nolte F, Yáñez-Mó M, Sánchez-Madrid F, Schinkel AH, Jalkanen S, Craig AG, Bruchhaus I, Horstmann RD.
Cell Microbiol. 2014 Jan 21. doi: 10.1111/cmi.12270

The Cell Surface Proteome of Entamoeba histolytica.
Biller L, Matthiesen J, Kühne V, Lotter H, Handal G, Nozaki T, Saito-Nakano Y, Schümann M, Roeder T, Tannich E, Krause E, Bruchhaus I.
Mol Cell Proteomics. 2014 Jan;13(1):132-44. doi: 10.1074/mcp.M113.031393.

All Publications

Laborgruppe Bruchhaus
2016

Octopamine controls starvation resistance, life span and metabolic traits in Drosophila.
Li Y, Hoffmann J, Li Y, Stephano F, Bruchhaus I, Fink C, Roeder T.
Sci Rep. 2016 Oct 19;6:35359.

Overexpression of Differentially Expressed Genes Identified in Non-pathogenic and Pathogenic Entamoeba histolytica Clones Allow Identification of New Pathogenicity Factors Involved in Amoebic Liver Abscess Formation.
Meyer M, Fehling H, Matthiesen J, Lorenzen S, Schuldt K, Bernin H, Zaruba M, Lender C, Ernst T, Ittrich H, Roeder T, Tannich E, Lotter H, Bruchhaus I.
PLoS Pathog. 2016 Aug 30;12(8):e1005853. doi: 10.1371/journal.ppat.1005853.

Social stress increases the susceptibility to infection in the ant Harpegnathos saltator
Schneider SA, Scharffetter C, Wagner AE, Boesch C, Bruchhaus I, Rimbach G, Roeder T.
Sci Rep. 2016 May 10;6:25800. doi: 10.1038/srep25800.

IL-23 promotes immunopathology and prevents IL-13-dependent tissue repair associated with Ly6Clo monocytes in Entamoeba histolytica-induced liver damage.
Noll J, Helk E, Fehling H, Bernin H, Marggraff C, Jacobs T, Huber S, Pelczar P, Ernst T, Ittrich H, Otto B, Mittrücker HW, Hölscher C, Tacke F, Bruchhaus I, Tannich E, Lotter H.
J Hepatol. 2016 May;64(5):1147-57. doi: 10.1016/j.jhep.2016.01.013.

Phylogeny of haemosporidian blood parasites revealed by a multi-gene approach.
Borner J, Pick C, Thiede J, Kolawole OM, Kingsley MT, Schulze J, Cottontail VM, Wellinghausen N, Schmidt-Chanasit J, Bruchhaus I, Burmester T.
Mol Phylogenet Evol. 2016 Jan;94(Pt A):221-31. doi: 10.1016/j.ympev.2015.09.003. Epub 2015 Sep 11.

Laborgruppe Bruchhaus
2015

Type of in vitro cultivation influences cytoadhesion, knob structure, protein localization and transcriptome profile of Plasmodium falciparum.
Tilly AK, Thiede J, Metwally N, Lubiana P, Bachmann A, Roeder T, Rockliffe N, Lorenzen S, Tannich E, Gutsmann T, Bruchhaus I.
Sci Rep. 2015 Nov 16;5:16766. doi: 10.1038/srep16766.

A comparative study of the localization and membrane topology of members of the RIFIN, STEVOR and PfMC-2TM protein families in Plasmodium falciparum-infected erythrocytes.
Bachmann A, Scholz JA, Janßen M, Klinkert MQ, Tannich E, Bruchhaus I, Petter M.
Malar J. 2015 Jul 16;14:274.

Magnetic Resonance Imaging of Pathogenic Protozoan Parasite Entamoeba histolytica Labeled With Superparamagnetic Iron Oxide Nanoparticles.
Ernst TM, Fehling H, Bernin H, Zaruba MD, Bruchhaus I, Adam G, Ittrich H, Lotter H.
Invest Radiol. 2015 Oct;50(10):709-18.

The Plasmodium falciparum exportome contains non-canonical PEXEL/HT proteins.
Schulze J, Kwiatkowski M, Borner J, Schlüter H, Bruchhaus I, Burmester T, Spielmann T, Pick C.
Mol Microbiol. 2015 Jul;97(2):301-14.

Cysteine peptidases in pathogenesis.
Bruchhaus I, Matthiesen J.
Amebiasis. Biology and Pathogenesis of Entamoeba. Nozaki, Tomoyoshi, Bhattacharya, Alok (Eds.), Springer, 2015. ISBN 978-4-431-55199-7.

Laborgruppe Bruchhaus
2014

Transcriptional regionalization of the fruit fly's airway epithelium
Faisal MN, Hoffmann J, El-Kholy S, Kallsen K, Wagner C, Bruchhaus I, Fink C, Roeder T.
PLoS One. 2014 Jul 14;9(7):e102534.

Evidence of promiscuous endothelial binding by Plasmodium falciparum-infected erythrocytes
Esser C, Bachmann A, Kuhn D, Schuldt K, Förster B, Thiel M, May J, Koch-Nolte F, Yáñez-Mó M, Sánchez-Madrid F, Schinkel AH, Jalkanen S, Craig AG, Bruchhaus I, Horstmann RD.
Cell Microbiol. 2014 May;16(5):701-8.

The cell surface proteome of Entamoeba histolytica
Biller L, Matthiesen J, Kühne V, Lotter H, Handal G, Nozaki T, Saito-Nakano Y, Schümann M, Roeder T, Tannich E, Krause E, Bruchhaus I.
Mol Cell Proteomics. 2014 Jan;13(1):132-44.

Lophophorata monophyletic – after all.
Nesnidal MP, Helmkampf M, Bruchhaus I, Ebersberg I, Hausdorf B.
Deep Metazoan Phylogeny: Backbone of the Tree of Life. 2014. Edited by Waegele JW, Bartholomaeus T, Misof B, Vogt L. Berlin: Walter de Gruyter.

Laborgruppe Bruchhaus
2013

New phylogenomic data support the monophyly of Lophophorata and an Ectoproct-Phoronid clade and indicate that Polyzoa and Kryptrochozoa are caused by systematic bias.
Nesnidal MP, Helmkampf M, Meyer A, Witek A, Bruchhaus I, Ebersberger I, Hankeln T, Lieb B, Struck TH, Hausdorf B.
BMC Evol Biol. 2013 Nov 17;13:253. doi: 10.1186/1471-2148-13-253.

Agent of whirling disease meets orphan worm: phylogenomic analyses firmly place Myxozoa in Cnidaria.
Nesnidal MP, Helmkampf M, Bruchhaus I, El-Matbouli M, Hausdorf B.
PLoS One. 2013;8(1):e54576. doi: 10.1371/journal.pone.0054576. Epub 2013 Jan 30.

Overexpression of specific cysteine peptidases confers pathogenicity to a nonpathogenic Entamoeba histolytica clone.
Matthiesen J, Bär AK, Bartels AK, Marien D, Ofori S, Biller L, Tannich E, Lotter H, Bruchhaus I.
MBio. 2013 Mar 26;4(2).

Laborgruppe Bruchhaus
2012

Genomic diversity of the human intestinal parasite Entamoeba histolytica.
Weedall GD, Clark CG, Koldkjaer P, Kay S, Bruchhaus I, Tannich E, Paterson S, Hall N.
Genome Biol. 2012 May 25;13(5):R38. doi: 10.1186/gb-2012-13-5-r38.

The cysteine protease inhibitors EhICP1 and EhICP2 perform different tasks in the regulation of endogenous protease activity in trophozoites of Entamoeba histolytica.
Šaric M, Irmer H, Eckert D, Bär AK, Bruchhaus I, Scholze H.
Protist. 2012 Jan;163(1):116-28. doi: 10.1016/j.protis.2011.01.003.

Temporal expression and localization patterns of variant surface antigens in clinical Plasmodium falciparum isolates during erythrocyte schizogony.
Bachmann A, Petter M, Tilly AK, Biller L, Uliczka KA, Duffy MF, Tannich E, Bruchhaus I.
PLoS One. 2012;7(11):e49540.

Entamoeba cysteine endopeptidases belonging to the C1 papain superfamily.
Scholze H, Tannich E, Bruchhaus I.
Handbook of Proteolytic Enzymes, 3nd edition, 2012. Rawlings N.D. and Salvesen, G., eds., Ch. 438, Elsevier, pp. 1915-1918.

Laborgruppe Bruchhaus
2011

Phylogenomic analyses of malaria parasites and evolution of their exported proteins.
Pick C, Ebersberger I, Spielmann T, Bruchhaus I, Burmester T.
BMC Evol Biol. 2011 Jun 15;11:167. doi: 10.1186/1471-2148-11-167.

The complete mitochondrial genome of Flustra foliacea (Ectoprocta, Cheilostomata) - compositional bias affects phylogenetic analyses of lophotrochozoan relationships.
Nesnidal MP, Helmkampf M, Bruchhaus I, Hausdorf B.
BMC Genomics. 2011 Nov 23;12:572. doi: 10.1186/1471-2164-12-572.

Highly co-ordinated var gene expression and switching in clinical Plasmodium falciparum isolates from non-immune malaria patients.
Bachmann A, Predehl S, May J, Harder S, Burchard GD, Gilberger TW, Tannich E, Bruchhaus I.
Cell Microbiol. 2011 Sep;13(9):1397-409.

Laborgruppe Bruchhaus
2010

Compositional heterogeneity and phylogenomic inference of metazoan relationships.
Nesnidal MP, Helmkampf M, Bruchhaus I, Hausdorf B.
Mol Biol Evol. 2010 Sep;27(9):2095-104. doi: 10.1093/molbev/msq097.

The essentials of protein import in the degenerate mitochondrion of Entamoeba histolytica.
Dolezal P, Dagley MJ, Kono M, Wolynec P, Liki? VA, Foo JH, Sedinová M, Tachezy J, Bachmann A, Bruchhaus I, Lithgow T.
PLoS Pathog. 2010 Mar 19;6(3):e1000812. doi: 10.1371/journal.ppat.1000812.

Characterization of a subunit of the outer dynein arm docking complex necessary for correct flagellar assembly in Leishmania donovani.
Harder S, Thiel M, Clos J, Bruchhaus I.
PLoS Negl Trop Dis. 2010 Jan 26;4(1):e586. doi: 10.1371/journal.pntd.0000586.

Differences in the transcriptome signatures of two genetically related Entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties.
Biller L, Davis PH, Tillack M, Matthiesen J, Lotter H, Stanley SL Jr, Tannich E, Bruchhaus I.
BMC Genomics. 2010 Jan 26;11:63. doi: 10.1186/1471-2164-11-63.

Phylogenetic relationships within the lophophorate lineages (Ectoprocta, Brachiopoda and Phoronida).
Hausdorf B, Helmkampf M, Nesnidal MP, Bruchhaus I.
Mol Phylogenet Evol. 2010 Jun;55(3):1121-7. doi: 10.1016/j.ympev.2009.12.022.

tRNA gene sequences are required for transcriptional silencing in Entamoeba histolytica.
Irmer H, Hennings I, Bruchhaus I, Tannich E.
Eukaryot Cell. 2010 Feb;9(2):306-14. doi: 10.1128/EC.00248-09.

Caenopores are antimicrobial peptides in the nematode Caenorhabditis elegans instrumental in nutrition and immunity.
Roeder T, Stanisak M, Gelhaus C, Bruchhaus I, Grötzinger J, Leippe M.
Dev Comp Immunol. 2010 Feb;34(2):203-9. doi: 10.1016/j.dci.2009.09.010.

Laborgruppe Bruchhaus
2009

Absence of erythrocyte sequestration and lack of multicopy gene family expression in Plasmodium falciparum from a splenectomized malaria patient.
Bachmann A, Esser C, Petter M, Predehl S, von Kalckreuth V, Schmiedel S, Bruchhaus I, Tannich E.
PLoS One. 2009 Oct 14;4(10):e7459. doi: 10.1371/journal.pone.0007459.

Comparison of two genetically related Entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties.
Biller L, Schmidt H, Krause E, Gelhaus C, Matthiesen J, Handal G, Lotter H, Janssen O, Tannich E, Bruchhaus I.
Proteomics. 2009 Sep;9(17):4107-20. doi: 10.1002/pmic.200900022.

Major cysteine peptidases of Entamoeba histolytica are required for aggregation and digestion of erythrocytes but are dispensable for phagocytosis and cytopathogenicity.
Irmer H, Tillack M, Biller L, Handal G, Leippe M, Roeder T, Tannich E, Bruchhaus I.
Mol Microbiol. 2009 May;72(3):658-67. doi: 10.1111/j.1365-2958.2009.06672.x.

Laborgruppe Bruchhaus
2008

An Entamoeba cysteine peptidase specifically expressed during encystation.
Ebert F, Bachmann A, Nakada-Tsukui K, Hennings I, Drescher B, Nozaki T, Tannich E, Bruchhaus I.
Parasitol Int. 2008 Dec;57(4):521-4. doi: 10.1016/j.parint.2008.07.002.

Phylogenomic analyses of lophophorates (brachiopods, phoronids and bryozoans) confirm the Lophotrochozoa concept.
Helmkampf M, Bruchhaus I, Hausdorf B.
Proc Biol Sci. 2008 Aug 22;275(1645):1927-33. doi: 10.1098/rspb.2008.0372.

Involvement of a Leishmania thymidine kinase in flagellum formation, promastigote shape and growth as well as virulence.
Thiel M, Harder S, Wiese M, Kroemer M, Bruchhaus I.
Mol Biochem Parasitol. 2008 Apr;158(2):152-62. doi: 10.1016/j.molbiopara.2007.12.005.

Characterization of a conserved rhoptry-associated leucine zipper-like protein in the malaria parasite Plasmodium falciparum.
Haase S, Cabrera A, Langer C, Treeck M, Struck N, Herrmann S, Jansen PW, Bruchhaus I, Bachmann A, Dias S, Cowman AF, Stunnenberg HG, Spielmann T, Gilberger TW.
Infect Immun. 2008 Mar;76(3):879-87. doi: 10.1128/IAI.00144-07.

Multigene analysis of lophophorate and chaetognath phylogenetic relationships.
Helmkampf M, Bruchhaus I, Hausdorf B.
Mol Phylogenet Evol. 2008 Jan;46(1):206-14.

Laborgruppe Bruchhaus
2007

Structure and content of the Entamoeba histolytica genome.
Clark CG, Alsmark UC, Tazreiter M, Saito-Nakano Y, Ali V, Marion S, Weber C, Mukherjee C, Bruchhaus I, Tannich E, Leippe M, Sicheritz-Ponten T, Foster PG, Samuelson J, Noël CJ, Hirt RP, Embley TM, Gilchrist CA, Mann BJ, Singh U, Ackers JP, Bhattacharya S, Bhattacharya A, Lohia A, Guillén N, Duchêne M, Nozaki T, Hall N.
Adv Parasitol. 2007;65:51-190.

Spiralian phylogenomics supports the resurrection of Bryozoa comprising Ectoprocta and Entoprocta.
Hausdorf B, Helmkampf M, Meyer A, Witek A, Herlyn H, Bruchhaus I, Hankeln T, Struck TH, Lieb B.
Mol Biol Evol. 2007 Dec;24(12):2723-9.

Protozoan parasites: programmed cell death as a mechanism of parasitism.
Bruchhaus I, Roeder T, Rennenberg A, Heussler VT.
Trends Parasitol. 2007 Aug;23(8):376-83.

The Entamoeba histolytica genome: primary structure and expression of proteolytic enzymes.
Tillack M, Biller L, Irmer H, Freitas M, Gomes MA, Tannich E, Bruchhaus I.
BMC Genomics. 2007 Jun 14;8:170.

Proteomic analysis of Entamoeba histolytica.
Tolstrup J, Krause E, Tannich E, Bruchhaus I.
Parasitology. 2007 Feb;134(Pt 2):289-98.

Laborgruppe Bruchhaus
2006

The second cysteine protease inhibitor, EhICP2, has a different localization in trophozoites of Entamoeba histolytica than EhICP1.
Saric M, Vahrmann A, Bruchhaus I, Bakker-Grunwald T, Scholze H.
Parasitol Res. 2006 Dec;100(1):171-4.

Increased expression of the major cysteine proteinases by stable episomal transfection underlines the important role of EhCP5 for the pathogenicity of Entamoeba histolytica.
Tillack M, Nowak N, Lotter H, Bracha R, Mirelman D, Tannich E, Bruchhaus I.
Mol Biochem Parasitol. 2006 Sep;149(1):58-64.

Expression of a mitochondrial peroxiredoxin prevents programmed cell death in Leishmania donovani.
Harder S, Bente M, Isermann K, Bruchhaus I.
Eukaryot Cell. 2006 May;5(5):861-70.

Laborgruppe Bruchhaus
2005

In vitro amoebicidal activity of some medicinal plants of the bambun region (Cameroon)
Moundipa PF, Melanie Flore KG, Bilong Bilong CF, Bruchhaus, I.
Afr. J. Trad. CAM 2005, 2, 113-121.

The genome of the protist parasite Entamoeba histolytica.
Loftus B, Anderson I, Davies R, Alsmark UC, Samuelson J, Amedeo P, Roncaglia P, Berriman M, Hirt RP, Mann BJ, Nozaki T, Suh B, Pop M, Duchene M, Ackers J, Tannich E, Leippe M, Hofer M, Bruchhaus I, Willhoeft U, Bhattacharya A, Chillingworth T, Churcher C, Hance Z, Harris B, Harris D, Jagels K, Moule S, Mungall K, Ormond D, Squares R, Whitehead S, Quail MA, Rabbinowitsch E, Norbertczak H, Price C, Wang Z, Guillén N, Gilchrist C, Stroup SE, Bhattacharya S, Lohia A, Foster PG, Sicheritz-Ponten T, Weber C, Singh U, Mukherjee C, El-Sayed NM, Petri WA Jr, Clark CG, Embley TM, Barrell B, Fraser CM, Hall N.
Nature. 2005 Feb 24;433(7028):865-8.

Identification of EhICP1, a chagasin-like cysteine protease inhibitor of Entamoeba histolytica.
Riekenberg S, Witjes B, Sari? M, Bruchhaus I, Scholze H.
FEBS Lett. 2005 Mar 14;579(7):1573-8.

Laborgruppe Bruchhaus
2004

Resistance of Entamoeba histolytica to the cysteine proteinase inhibitor E64 is associated with secretion of pro-enzymes and reduced pathogenicity.
Nowak N, Lotter H, Tannich E, Bruchhaus I.
J Biol Chem. 2004 Sep 10;279(37):38260-6.

A peroxiredoxin specifically expressed in two types of pharyngeal neurons is required for normal growth and egg production in Caenorhabditis elegans.
Isermann K, Liebau E, Roeder T, Bruchhaus I.
J Mol Biol. 2004 May 7;338(4):745-55.

Entamoeba histolytica trophozoites transfer lipophosphopeptidoglycans to enteric cell layers.
Lauwaet T, Oliveira MJ, De Bruyne G, Bruchhaus I, Duchêne M, Mareel M, Leroy A.
Int J Parasitol. 2004 Apr;34(5):549-56.

Effects of bisphosphonates on the growth of Entamoeba histolytica and Plasmodium species in vitro and in vivo.
Ghosh S, Chan JM, Lea CR, Meints GA, Lewis JC, Tovian ZS, Flessner RM, Loftus TC, Bruchhaus I, Kendrick H, Croft SL, Kemp RG, Kobayashi S, Nozaki T, Oldfield E.
J Med Chem. 2004 Jan 1;47(1):175-87.

Laborgruppe Bruchhaus
2003

Developmentally induced changes of the proteome in the protozoan parasite Leishmania donovani.
Bente M, Harder S, Wiesgigl M, Heukeshoven J, Gelhaus C, Krause E, Clos J, Bruchhaus I.
Proteomics. 2003 Sep;3(9):1811-29.

The intestinal protozoan parasite Entamoeba histolytica contains 20 cysteine protease genes, of which only a small subset is expressed during in vitro cultivation.
Bruchhaus I, Loftus BJ, Hall N, Tannich E.
Eukaryot Cell. 2003 Jun;2(3):501-9.

Laborgruppe Bruchhaus
2002

Differential gene expression in Entamoeba histolytica isolated from amoebic liver abscess.
Bruchhaus I, Roeder T, Lotter H, Schwerdtfeger M, Tannich E.
Mol Microbiol. 2002 May;44(4):1063-72.

Recombinant expression and purification of an enzymatically active cysteine proteinase of the protozoan parasite Entamoeba histolytica.
Hellberg A, Nowak N, Leippe M, Tannich E, Bruchhaus I.
Protein Expr Purif. 2002 Feb;24(1):131-7.

Laborgruppe Bruchhaus
2001

Comparative proteome analysis of Leishmania donovani at different stages of transformation from promastigotes to amastigotes.
Thiel M, Bruchhaus I.
Med Microbiol Immunol. 2001 Nov;190(1-2):33-6.

Introns of Entamoeba histolytica and Entamoeba dispar.
Willhoeft U, Campos-Góngora E, Touzni S, Bruchhaus I, Tannich E.
Protist. 2001 Jul;152(2):149-56.

Overexpression of cysteine proteinase 2 in Entamoeba histolytica or Entamoeba dispar increases amoeba-induced monolayer destruction in vitro but does not augment amoebic liver abscess formation in gerbils.
Hellberg A, Nickel R, Lotter H, Tannich E, Bruchhaus I.
Cell Microbiol. 2001 Jan;3(1):13-20.

Laborgruppe Bruchhaus
2000

A simple and reliable 5'-RACE approach.
Schramm G, Bruchhaus I, Roeder T.
Nucleic Acids Res. 2000 Nov 15;28(22):E96.

Superoxide dismutase reduces susceptibility to metronidazole of the pathogenic protozoan Entamoeba histolytica under microaerophilic but not under anaerobic conditions.
Wassmann C, Bruchhaus I.
Arch Biochem Biophys. 2000 Apr 1;376(1):236-8.

Two major 'higher molecular mass proteinases' of Entamoeba histolytica are identified as cysteine proteinases 1 and 2.
Hellberg A, Leippe M, Bruchhaus I.
Mol Biochem Parasitol. 2000 Feb 5;105(2):305-9.

Laborgruppe Bruchhaus
1998

Isolation of ultrapure supercoiled plasmid-DNA using preparative electrophoresis.
Roeder T, Görich D, Roeder NP, Bruchhaus I.
Electrophoresis. 1998 Jul;19(10):1575-6.

Recombinant expression and biochemical characterization of an NADPH:flavin oxidoreductase from Entamoeba histolytica.
Bruchhaus I, Richter S, Tannich E.
Biochem J. 1998 Mar 15;330 ( Pt 3):1217-21.

Isolation and molecular characterization of a surface-bound proteinase of Entamoeba histolytica.
Jacobs T, Bruchhaus I, Dandekar T, Tannich E, Leippe M.
Mol Microbiol. 1998 Jan;27(2):269-76.

Laborgruppe Bruchhaus
1997

Removal of hydrogen peroxide by the 29 kDa protein of Entamoeba histolytica.
Bruchhaus I, Richter S, Tannich E.
Biochem J. 1997 Sep 15;326 ( Pt 3):785-9.

Characterization of two E. histolytica proteins that inactivate reactive oxygen species.
Bruchhaus I, Richter S, Tannich E.
Arch Med Res. 1997;28 Spec No:91-2.

Problematik der Isolierung genomischer DNA.
Bruchhaus I, Müller H-J
BIOspektrum, 1997, 1, 68 + 73.

Laborgruppe Bruchhaus
1995

Free radicals and protozoa.
Bruchhaus I, Dive D.
Protistol Actual. 1995, 195-203.

Identification of an Entamoeba histolytica gene encoding a protein homologous to prokaryotic disulphide oxidoreductases.
Bruchhaus I, Tannich E.
Mol Biochem Parasitol. 1995 Mar;70(1-2):187-91.

Laborgruppe Bruchhaus
1993

Unusual gene organization in the protozoan parasite Entamoeba histolytica.
Bruchhaus I, Leippe M, Lioutas C, Tannich E.
DNA Cell Biol. 1993 Dec;12(10):925-33.

Primary structure of the pyruvate phosphate dikinase in Entamoeba histolytica.
Bruchhaus I, Tannich E.
Mol Biochem Parasitol. 1993 Nov;62(1):153-6.

Analysis of the genomic sequence encoding the 29-kDa cysteine-rich protein of Entamoeba histolytica.
Bruchhaus I, Tannich E.
Trop Med Parasitol. 1993 Jun;44(2):116-8.

Laborgruppe Bruchhaus
1992

Purification of glutathione reductase from muscle of the adult parasitic nematode Ascaris suum.
Komuniecki R, Bruchhaus I, Ilg T, Wilson K, Zhang Y, Fairlamb AH.
Mol Biochem Parasitol. 1992 Apr;51(2):331-3.

Recombinant expression, purification and biochemical characterization of a superoxide dismutase from Entamoeba histolytica.
Bruchhaus I, Brattig NW, Tannich E.
Arch Med Res. 1992;23(2):27-9.

Laborgruppe Bruchhaus
1991

Pathogenic and nonpathogenic Entamoeba histolytica: identification and molecular cloning of an iron-containing superoxide dismutase.
Tannich E, Bruchhaus I, Walter RD, Horstmann RD.
Mol Biochem Parasitol. 1991 Nov;49(1):61-71.

News from the group

Finally - it - is - done. CONGRATULATIONS Dr. Martin Meyer!!!!! (13.10.2016)

Congratulations Dr. rer. nat. Nahla Metwally

 

 

 

 

BioMalParXII: Biology and Pathology of the Malaria Parasite, Heidelberg, 18-20 May 2016

27th Annual Meeting of the German Society for Parasitology, Göttingen, 9-12 March 2016

 

 

 

 

Alumni

Doctoral Thesis

Dr. rer. nat. Martin Meyer (2013-2016) : Identifizierung neuer Pathogenitätsfaktoren durch Überexpression differentiell exprimierter Gene in pathogenen und nicht-pathogenen Entamoeba histolytica (SCHAUDINN, 1903) Klonen.

Dr. rer. nat. Nahla Metwally (2013-2016) : Analyses of the transcriptome profiles of Plasmodium falciparum infected erythrocytes selected for binding to the human endothelial receptors (ICAM-1, P-selectin, E-selectin, CD9 and CD151).

Dr. rer. nat. Helena Fehling (2012-2016) : Analyse der Unterschiede pathogener und apathogener Entamoeba histolytica (SCHAUDINN, 1903) Klone in der Parasit-Wirt Interaktion.

Dr. rer. nat. Ann-Kathrin Tilly (2012-2015) : Adhäsion von Plasmodium falciparum (Welch, 1897) infizierten Erythrozyten an den humanen Endothelrezeptor P-Selektin.

Dr. rer. nat. Maximilian Nesnidal (2009-2013) : Molekularphylogenetische Analyse basaler Linien der Bilateria.

Dr. rer. nat. Jenny Matthiesen (2009-2012) : Charakterisierung des pathogenen Phänotyps von Entamoeba histolytica (Schaudinn, 1903).

Dr. rer. nat. Ghassan Handal (2007-2010) : Regulation of antioxidant enzymes in Entamoeba histolytica (Schaudinn, 1903).

Dr. rer. nat. Anna Bachmann (2007-2010) : Genexpression und Lokalisation variabler Oberflächenproteine des Malariaerregers Plasmodium falciparum (Welch, 1897).

Dr. rer. nat. Sabine Predehl (2007-2010) : Funktionsanalyen der PfEMP1 Proteinfamilie von Plasmodium falciparum (Welch, 1897).

Dr. rer. nat. Laura Biller (2006-2009) : Identifizierung der Pathogenitätsfaktoren von Entamoeba histolytica mittels Phänotypisierung und vergleichender Expressionsanalysen.

Dr. rer. nat. Martin Helmkampf (2005-2009) : Molecular phylogenetic analysis of Lophophorata and Chaetognatha.

Dr. rer. nat. Manuela Tillack (2004-2008) : Die Bedeutung der Cystein-Proteinasen des parasitischen Protozoons Entamoeba histolytica für die Phagozytose, Gewebszerstörung, Invasion und Abszessbildung.

Dr. rer. nat. Meike Thiel (2000-2006) : Identifizierung und Charakterisierung stadienspezifischer Proteine von Leishmania donovani.

Dr. rer. nat. Simone Harder (2001-2005) : Proteomanalyse der Stadiendifferenzierung von Leishmania donovani.

Dr. rer. nat. Kerstin Isermann (2001-2004) : Charakterisierung der Peroxiredoxine von Caenorhabditis elegans.

Dr. rer. nat. Nicolas Nowak (2000-2004) : Charakterisierung der Peptidasen von Entamoeba histolytica.

Dr. rer. nat. Claudia Waßmann (1997-2000) : Charakterisierung enzymatischer Antioxidantien von Entamoeba histolytica.

Dr. rer. nat. Andrea Hellberg (1997-2000) : Cystein-Proteinasen von Entamoeba histolytica und Entamoeba dispar.

Dr. med. Symi Richter (1996) : Rekombinante Expression und funktionelle Charakterisierung eines Peroxiredoxins und einer NADPH:Flavin-Oxidoreduktase des Protozoons Entamoeba histolytica.

Diploma and Master Thesis

Marius Schmitt (2016) : Localization of Plasmodium vivax (Grassi and Feletti, 1890) VIR proteins by means of Plasmodium falciparum (Welch, 1897) transgenic cell lines.

Michael Dörpinghaus (2015) : Development of a system to characterize the binding properties of Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) domains to human endothelial receptors

Eugenia Reit (2015) : Production of transgenic CHO–cell lines for investigation of interaction of Plasmodium falciparum with the human endothelial receptors VCAM–1, PECAM–1 and VE–cadherin.

Lisa Roth (2015) : Characterization of binding characteristics of human endothelial receptors among human erythrocytes infected with malaria parasite Plasmodium falciparum.

Ellen Drews (2014) : Etablierung lentiviraler Expressionssysteme zur Charakterisierung der Interaktion zwischen Plasmodium falciparum (Welch, 1897) infizierten Erythrozyten und humanen Endothelrezeptoren.

Jenny Thiede (2013) : Lokalisierung ausgewählter Proteine mit alternativen, putativen PEXEL-Motiv des Malariaerregers Plasmodium falciparum (Welch, 1897).

Anne Bartels (2013) : Die Überexpression von Cysteinpeptidasen beeinflusst die Virulenz nicht-pathogener Entamoeba histolytica [SCHAUDINN, 1903] Trophozoiten.

Vera Kühne (2012) : Charakterisierung des putativen Membranproteoms von Entamoeba histolytica (Schaudinn, 1903).

Judith Scholz (2012) : Genexpression und Lokalisation variabler Oberflächenproteine in Merozoiten des Malariaerregers Plasmodium falciparum (Welch, 1897).

Olga Shpigelman (2011) : Identification of binding domains of PfEMP1 molecules to the receptor ICAM–1 of Plasmodium falciparum (Welch, 1897).

Ann-Kathrin Tilly (2011) : Expression und Lokalisation variabler Oberflächenantigene des Malariaerregers Plasmodium falciparum (Welch, 1897) während der erythrozytären Schizogonie.

Ann-Kathrein Bär (2010) : Expressionsprofil der Cysteinpeptidasen von Entamoeba histolytica (SCHAUDINN 1903) während der Leberabszessbildung im experimentellen Mausmodell (Mus musculus, LINNAEUS 1758).

Johanna Anige (2010) : Charakterisierung der Bindungseigenschaften ausgewählter Domänen der PfEMP1-Moleküle von Plasmodium falciparum (Welch, 1897) mit Hilfe der Biacore-Technologie.

Steffi Renk (2010) : Adhärenz Plasmodium falciparum (Welch, 1897) infizierter Erythrozyten an Endothelrezeptoren: Einfluss der in vitro-Kultivierung.

Babette Drescher (2009) : Charakterisierung und Lokalisierung ausgewählter Mitglieder der aig-Genfamilie von Entamoeba histolytica (Schaudinn, 1903).

Jenny Matthiesen (2008) : Charakterisierung putativer EhAIG-Proteine und Aufbau eines Protein-Markierungssystems bei Entamoeba histolytica (Schaudinn, 1903).

Dennis Marien (2007) : Expression der Peptidasen und weiterer putativer Pathogenitätsfaktoren von Entamoeba histolytica während der Leberabszessbildung.

Nina Alex (2006) : Expressionsstudien von CudA-homologen (culmination defective) Genen in Entamoeba histolytica (Schaudinn, 1903) und Entamoeba invadens (Rodhain, 1934).

Maya Kono (2007) : Charakterisierung putativer mitosomaler Proteine in Entamoeba histolytica.

Sabine Predehl (2007) : Dynamik der var-Genexpression bei Plasmodium falciparum.

Anna Bachmann (2006) : Analyse der var-Genexpression bei Plasmodium falciparum.

Mona Shahhossini (2004) : Regulation der Cystein-Proteinasen von Entamoeba invadens während der Enzystierung.

Jörn Tolstrup (2004) : Proteomanalyse Cystein-Proteinase überexprimierender Entamoeba histolytica.

Verena Diehm (2001) : Analyse der Expressionsmuster der Proteasegene des parasitischen Protozoons Entamoeba histolytica.

Simone Harder (2000) : Untersuchungen zur Inhibition der Proteinsynthese durch "Antisense"-RNA und RNA"-Interference" bei Entamoeba histolytica.

Meike Thiel (1999) : Identifizierung stadienspezifisch exprimierter Proteine des parasitischen Protozoons Leishmania donovani.

Bachelor Thesis

Elena Baron (2015) : Localization of Plasmodium vivax (Grassi and Feletti, 1890) VIR proteins by means of Plasmodium falciparum (Welch, 1897) transgenic cell lines.

Postdocs

Dr. rer. nat. Jenny Matthiesen (2012-2016) : Pathogenicity factors and cysteine peptidases of Entamoeba histolytica