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All re-examinations for known pathogens again were negative, also in the squirrels. Finally, colleagues from the Friedrich Loeffler Institute sequenced all RNA genetic material from the brain of a squirrel and were successful. In addition to the squirrel’s mammalian genes, sequences were found that clearly originated from a virus. Virus-specific tests, designed based on these sequences, revealed the presence of the new virus in several squirrels and, notably, in the brains and cerebrospinal fluids of the deceased breeders. The new virus, a Borna virus, clearly differs from all Borna viruses identified so far, most of which cause encephalitis in horses. The new virus had presumably been transmitted to the breeders by bites or scratches of the squirrels.
Dennis Tappe, Daniel Cadar, Jonas Schmidt-Chanasit and external co-operation partners (see publication)