Well-functioning and sustainable laboratory services are key components of strong health systems and crucial for improving public health and research capacities of countries. Lab-SPHERE - Laboratory Systems and Public Health in Resource-Limited Settings is a tropEd accredited 2-week intensive course offered annually since 2019 aimed at describing the role of laboratory systems in strengthening health services and research capacity with a special focus on low and middle-income countries (LMICs). The emphasis is on explaining the structure and functions of laboratory systems and their effective and efficient management. Moreover, areas where laboratory systems overlap and/or complement health systems are being considered. The course uses interactive, simulation-based exercises, as well as laboratory- and computer-based practical sessions to complement the theoretical content.