Arbeitgruppe: Abteilung Molekulare Parasitologie
Abteilung Molekulare Parasitologie

Overview

The Department of Molecular Parasitology focuses its research on molecular aspects of amoebiasis and malaria, two parasitic diseases with high impact on morbidity and mortality in tropical countries. In particular, questions concerning variability, immune evasion and pathogenicity of Entamoeba histolytica and Plasmodium falciparum are investigated. In addition, the Department is involved in mosquito surveillance and pathogen detection as well as in the development of new tools to improve diagnostics of parasite infections. In collaboration with the Institute for Standardisation and Documentation for Medical Laboratories (INSTAND) the Department regularly performs external quality assessments of more than 300 laboratories in the diagnostics of faecal and blood parasites.

Research Projects

Immune mechanisms underlying development and regeneration of amebic liver abscess

Lotter laboratory

Human infections with the protozoan parasite Entamoeba histolytica may result in severe focal lesions of the liver, also known as amebic liver abscess. Since the disease occurs mainly in adult men, it is obvious that in addition to parasite-specific effector molecules, host factors may also contribute to the outcome of the infection. We recently showed that the male sex hormone testosterone plays an important role in the susceptibility to the disease by suppressing protective immune responses. Hence, the prolonged persistence of the parasite in the liver of male individuals leads to an overwhelming immune response responsible for tissue damage. At present, we investigate immunologic and metabolic repair mechanisms of the liver and focus on molecular mechanisms underlying the sex disparity in the immune response.

Development of an immunostimulatory molecule for the treatment of cutaneous leishmaniasis

Lotter laboratory

The protozoan parasite Leishmania major (L. major) is the main causative agent of cutaneous leishmaniasis and is responsible for more than 1.5 million cases per year. Up to now, a convincing treatment regiment is not available. We recently isolated a glycolipid from the membrane of Entamoeba histolytica that exhibits strong immunostimulatory properties. In vitro and in vivo studies revealed that treatment with the membrane anchor of this molecule was effective against intracellular Leishmania growth resulting in a reduction of Leishmania-induced lesions in a mouse model for the disease. At present, we are investigating synthetic, modified versions of the molecule for their leishmanicidal activity, explore the molecular mechanism underlying the protective efficacy and develop suitable nanocarriers to improve bioavailability and clinical application of the compounds.

 

Understanding the pathogenicity of Entamoeba histolytica

Bruchhaus laboratory

Infections with Entamoeba histolytica may result in invasive diseases such as amoebic colitis or amoebic liver abscesses. However, the mechanisms underlying amoeba virulence are still unclear. Using a variety of molecular and imaging approaches, we are comparing E. histolytica clones, derived from the same parasite isolate but with different pathogenic phenotypes to unravel the molecular mechanisms that might be instrumental for amoeba virulence. New technical achievements such as mRNA sequencing, small animal-magnet resonance imaging, generation of genetically engineered amoeba cell lines, and intravital microscopy will be employed.

Plasmodium falciparum cytoadhesion: Characterization of receptor – ligand interactions

Bruchhaus laboratory

The human malaria parasite P. falciparum is able to avoid spleen-dependent killing by adhesion of infected erythrocytes (IEs) to human endothelial receptors that line microvascular venules. This cytoadhesion of IEs is the major reason for the most severe pathological phenotypes observed in malaria, including obstruction of blood flow, hypoxia, tissue damage and ultimately, organ failure. Although the phenomenon that IEs cytoadhere to endothelial receptors is central for the understanding of malaria pathogenesis, our current knowledge regarding the underlying molecular framework is far from being complete. To understand the phenomenon of P. falciparum cytoadhesion in more detail, we are searching for respective P. falciparum ligands mediating binding to specific receptors, map respective binding domains and analyse the molecular nature of P. falciparum cytoadhesion.

Expression and regulation of virulence-associated gene families in P. falciparum patient isolates

Anna Bachmann, Heidrun von Thien

The virulence of P. falciparum has been linked to the ability of infected erythrocytes to adhere to a range of endothelial cell surface molecules expressed on blood vessel walls such as CD36 and ICAM-1. This sequestration process allows the parasite to avoid spleen-dependent killing mechanisms. However, membrane proteins mediating sequestration are exposed to the host’s immune system and need to be varied in a long-lasting chronic infection by undergoing antigenic variation. For this reason P. falciparum possesses several multicopy gene families coding for variant surface antigens, which are characterized by an extensive sequence polymorphism. The best known is the high molecular weight PfEMP1 family (P. falciparum erythrocyte membrane protein 1), which is encoded by 60 var genes per parasite genome. Each var gene encodes a similar, but not identical PfEMP1 version with particular receptor binding capacities. The expression switching between different var genes enables the parasite to change its immunological appearance and cytoadhesion capability during the course of infection. Accordingly, blocking the expression switch between different PfEMP1 variants exposed on the surface of infected erythrocytes would give the immune system a major advantage in fighting malaria infection. For this reason the rate and mechanism at which parasites switch var expression to undergo antigenic variation in vivo is of tremendous interest and we are currently following several strategies to address this questions:

A. var gene expression at the early onset of P. falciparum blood stage infection:

We took advantage of a study conducted at the University Hospital in Tübingen, Germany, in which healthy, non-immune volunteers were infected with one lot of purified, cryopreserved NF54 sporozoites, the infectious mosquito stage of the parasite, provided by Sanaria, USA. After blood sampling, the RNA of the circulating ring stage parasites was immediately preserved for transcript profiling of the entire NF54 full var gene repertoire by quantitative real time PCR (qPCR). Currently we apply this qPCR approach to ex vivo parasites from 18 volunteers at consecutive time-points after sporozoite infection. In addition, we aim to compare var gene expression levels in parasites prior to mosquito infection with those detected in in vivo parasites. This analysis should allow us to answer (1) if an intrinsic program of the parasite determines the expression of the major virulence factor PfEMP1 at the early onset of P. falciparum blood infections in malaria-naïve individuals and (2) if this expression program is reset during mosquito or human liver passage of the parasite.

B. Profiling the expression dynamics of virulence-associated genes in clinical P. falciparum isolates:

 

In malaria endemic countries the expression of a var gene subset coding for particular PfEMP1 domain cassettes, which bind a novel endothelial receptor, were recently shown to be associated with severe malaria in young children. We pursue a novel strategy to identify biological processes involved in this host-pathogen interaction by analysing naturally occurring human P. falciparum infections in returning travellers recruited at the Bernhard Nocht Clinic in Hamburg. Importantly, gene expression in parasites from these patients is unaffected by pre-formed immune responses and can provide larger sample volumes for in-depth analyses. We use next generation RNA sequencing to identify and quantify all transcribed PfEMP1 domains To check (1) if non-immune adults hospitalized with clinical malaria symptoms mimic African children or (2) if different PfEMP1 domain cassettes are responsible for parasites adhesion in these patients, Furthermore, we analysing shortly cultivated parasites over several consecutive parasite generations to monitor (3) if the parasites switch their var gene expression to a different gene copy in absence of any immune pressure. Collaboration partners: Michael F. Duffy and Michaela Petter, University of Melbourne; Benjamin Mordmüller and Peter G. Kremsner, University of Tübingen; Matthias Frank, University of Tübingen.

Characterizing small variant surface antigens in regard to their gene expression, subcellular localization and function in different life cycle stages of the malaria parasite

Judith A. M. Scholz, Heidrun von Thien, Anna Bachmann

The malaria parasite invades and multiplies within red blood cells of its human host, thereby causing disease through anemia and obstruction of blood flow. Accordingly, parasites invasion of red blood cell, their adhesion to small vessel walls and immune evasion by means of antigenic variation are critical to malaria pathogenesis. For all processes an involvement of large protein families encoded in parasite’s genome is discussed. In addition to PfEMP1, which certainly mediates antigenic variation and endothelial receptor binding of P. falciparum, much less is known about the small variant surface antigens families RIFIN, STEVOR and PfMC-2TM. Interestingly, they members have been monitored at different subcellular sites within the infected red blood cell and are, therefore, candidates for mediating several of the patho-physiological events during malaria disease. However, a better understanding of these small variant surface protein families is necessary and may reveal new targets for intervention. To get more insight in their function, we established analytical tools for analyzing parasites obtained from malaria patients, whose gene expression and protein localization is not influenced by prolonged in vitro-cultivation. We could already show that the gene expression of RIFIN- and STEVOR-encoding genes is enhanced in vivo and that proteins of both families contribute to the surface coat of the infected red blood cell. Currently, we are characterizing the gene expression, localization and functional role of small variant surface antigen in the invasive stage, the merozoite, as well as in other life cycle stages of the parasite.

Collaboration partners: Michaela Petter, University of Melbourne

Entomology – Risk assessment of German mosquitoes

Mayke Leggewie, Heidrun von Thien, Anna Heitmann

As a result of intensified globalization of international trade and of substantial travel activities, mosquito-borne exotic pathogens are becoming an increasing threat for Europe. In Germany some 50 different mosquito species are known, several of which have vector competence for pathogens. In upcoming years the risk for arbovirus transmission might increase in Germany due to increased occurrence of invasive mosquito species, such as the Asian bush mosquito Ochlerotatus japonicus or the Asian tiger mosquito Aedes albopictus. These invasive species are characterized by high vector competence for a broad range of pathogens and a preference for human blood meals. For risk assessment, a number of mosquito and pathogen surveillance projects have been initiated in Germany during the last few years. Accordingly, the department is involved in (i) surveillance of Aedes albopictus in Southern Germany, (ii) studies on vector competence of indigenous mosquitoes for tropical viruses, making use of the BNITM BSL3-insectary, (iii) analysing the genetic variability of German mosquito species and last but not least (iv) screening German mosquitoes for parasite infections (e.g. dirofilaria).

Development of new diagnostics for the detection of parasite infections

Heidrun von Thien

For more than a century the diagnosis of parasites has relied upon microscopic inspection of stool samples. Recent developments of antigen-detection and DNA-based technologies have indicated that stool microscopy has a rather low sensitivity and is unable to differentiate between closely related pathogenic or non-pathogenic species or between subtypes of the same species. During the last few years our laboratory has developed assays that allow sensitive and specific detection of the various medically important parasites. These assays, which comprise antigen-detection, fluorescence in-situ hybridization and real-time PCR are extensively evaluted using comprehensive collections of clinical samples from travellers of the Bernhard Nocht Clinic as well as during large epidemiological studies on the prevalence and incidence of parasite infections in Africa and Asia. These assays are constantly optimized.

Publication Highlights

AMOEBIASIS
Abteilung Molekulare Parasitologie

Overexpression of Differentially Expressed Genes Identified in Non-pathogenic and Pathogenic Entamoeba histolytica Clones Allow Identification of New Pathogenicity Factors Involved in Amoebic Liver Abscess Formation.
Meyer M, Fehling H, Matthiesen J, Lorenzen S, Schuldt K, Bernin H, Zaruba M, Lender C, Ernst T, Ittrich H, Roeder T, Tannich E, Lotter H, Bruchhaus I.
PLoS Pathog. 2016 Aug 30;12(8):e1005853. doi: 10.1371/journal.ppat.1005853.

IL-23 promotes immunopathology and prevents IL-13-dependent tissue repair associated with Ly6Clo monocytes in Entamoeba histolytica-induced liver damage.
Noll J, Helk E, Fehling H, Bernin H, Marggraff C, Jacobs T, Huber S, Pelczar P, Ernst T, Ittrich H, Otto B, Mittrücker HW, Hölscher C, Tacke F, Bruchhaus I, Tannich E, Lotter H.
J Hepatol. 2016 May;64(5):1147-57. doi: 10.1016/j.jhep.2016.01.013.

The cell surface proteome of Entamoeba histolytica
Biller l, Matthiesen J, Kühne V, Lotter H, Handal G, Nozaki T, Saito-Nakano Y, Schürmann M, Roeder T, Tannich E, Krause E, Bruchhaus I.
Mol Cell Protomics. 2014; 13(1):132-144. doi: 10.1074/mcp.M113.031393

Testosterone increases susceptibility to amebic liver abscess in mice and mediates inhibition of IFN? secretion in natural killer T cells.
Lotter H, Helk E, Bernin H, Jacobs T, Prehn C, Adamski J, González-Roldán N, Holst O, Tannich E.
PLoS One. 2013;8(2):e55694. doi: 10.1371/journal.pone.0055694. Epub 2013 Feb 12.

TNFalpha-mediated liver destruction by Kupffer cells and Ly6Chi monocytes during Entamoeba histolytica infection.
Helk E, Bernin H, Ernst T, Ittrich H, Jacobs T, Heeren J, Tacke F, Tannich E, Lotter H.
PLoS Pathog. 2013 Jan;9(1):e1003096. doi: 10.1371/journal.ppat.1003096. Epub 2013 Jan 3.

MALARIA

Mosquito Passage Dramatically Changes var Gene Expression in Controlled Human Plasmodium falciparum Infections.
Bachmann A, Petter M, Krumkamp R, Esen M, Held J, Scholz JA, Li T, Sim BK, Hoffman SL, Kremsner PG, Mordmüller B, Duffy MF, Tannich E.
PLoS Pathog. 2016 Apr 12;12(4):e1005538. doi: 10.1371/journal.ppat.1005538.

Type of in vitro cultivation influences cytoadhesion, knob structure, protein localization and transcriptome profile of Plasmodium falciparum.
Tilly AK, Thiede J, Metwally N, Lubiana P, Bachmann A, Roeder T, Rockliffe N, Lorenzen S, Tannich E, Gutsmann T, Bruchhaus I.
Sci Rep. 2015 Nov 16;5:16766. doi: 10.1038/srep16766.

A comparative study of the localization and membrane topology of members of the RIFIN, STEVOR and PfMC-2TM protein families in Plasmodium falciparum-infected erythrocytes.
Bachmann A, Scholz JA, Janßen M, Klinkert MQ, Tannich E, Bruchhaus I, Petter M.
Malar J. 2015 Jul 16;14:274. doi: 10.1186/s12936-015-0784-2.

Evidence of promiscuous endothelial binding by Plasmodium falciparum-infected erythrocytes.
Esser C, Bachmann A, Kuhn D, Schuldt K, Förster B, Thiel M, May J, Koch-Nolte F, Yáñez-Mó M, Sánchez-Madrid F, Schinkel AH, Jalkanen S, Craig AG, Bruchhaus I, Horstmann RD.
Cell Microbiol. 2014 May;16(5):701-8. doi: 10.1111/cmi.12270.

Temporal expression and localization patterns of variant surface antigens in clinical Plasmodium falciparum isolates during erythrocyte schizogony.
Bachmann A, Petter M, Tilly AK, Biller L, Uliczka KA, Duffy MF, Tannich E, Bruchhaus I.
PLoS One. 2012;7(11):e49540. doi: 10.1371/journal.pone.0049540.

ENTOMOLOGY

Distribution of individual members of the mosquito Anopheles maculipennis complex in Germany identified by newly developed real-time PCR assays.
Lühken R, Czajka C, Steinke S, Jöst H, Schmidt-Chanasit J, Pfitzner W, Becker N, Kiel E, Krüger A, Tannich E.
Med Vet Entomol. 2016 Jan 20. doi: 10.1111/mve.12161.

Stable transmission of Dirofilaria repens nematodes, northern Germany.
Czajka C, Becker N, Jöst H, Poppert S, Schmidt-Chanasit J, Krüger A, Tannich E.
Emerg Infect Dis. 2014 Feb;20(2):328-31. doi: 10.3201/eid2002.131003.

Field evaluation of four widely used mosquito traps in Central Europe.
Lühken R, Pfitzner WP, Börstler J, Garms R, Huber K, Schork N, Steinke S, Kiel E, Becker N, Tannich E, Krüger A.
Parasit Vectors. 2014 Jun 12;7:268. doi: 10.1186/1756-3305-7-268.

Repeated introduction of Aedes albopictus into Germany, July to October 2012.
Becker N, Geier M, Balczun C, Bradersen U, Huber K, Kiel E, Krüger A, Lühken R, Orendt C, Plenge-Bönig A, Rose A, Schaub GA, Tannich E.
Parasitol Res. 2013 Apr;112(4):1787-90. doi: 10.1007/s00436-012-3230-1.

First nationwide surveillance of Culex pipiens complex and Culex torrentium mosquitoes demonstrated the presence of Culex pipiens biotype pipiens/molestus hybrids in Germany.
Rudolf M, Czajka C, Börstler J, Melaun C, Jöst H, von Thien H, Badusche M, Becker N, Schmidt-Chanasit J, Krüger A, Tannich E, Becker S.
PLoS One. 2013 Sep 11;8(9):e71832. doi: 10.1371/journal.pone.0071832.

DIAGNOSTICS

Tropheryma whipplei in children with diarrhoea in rural Ghana.
Vinnemeier CD, Klupp EM, Krumkamp R, Rolling T, Fischer N, Owusu-Dabo E, Addo MM, Adu-Sarkodie Y, Käsmaier J, Aepfelbacher M, Cramer JP, May J, Tannich E.
Clin Microbiol Infect. 2016 Jan;22(1):65.e1-3. doi: 10.1016/j.cmi.2015.09.022.

Human case of Onchocerca lupi infection, Germany, August 2014.
Bergua A, Hohberger B, Held J, Muntau B, Tannich E, Tappe D.
Euro Surveill. 2015 Apr 23;20(16). pii: 21099.

Fatal Human Meningoencephalitis due to Halicephalobus Nematodes, Germany.
Monoranu CM, Müllges W, Keppler M, Brehm K, Ondrejka SL, Muntau B, Tannich E, Müller-Hermelink HK, Tappe D.
Open Forum Infect Dis. 2015 May 22;2(2):ofv061. doi: 10.1093/ofid/ofv061.

A case of autochthonous human Dirofilaria infection, Germany, March 2014.
Tappe D, Plauth M, Bauer T, Muntau B, Dießel L, Tannich E, Herrmann-Trost P.
Euro Surveill. 2014 May 1;19(17):2-4.

Prospective European-wide multicentre study on a blood based real-time PCR for the diagnosis of acute schistosomiasis.
Wichmann D, Poppert S, Von Thien H, Clerinx J, Dieckmann S, Jensenius M, Parola P, Richter J, Schunk M, Stich A, Zanger P, Burchard GD, Tannich E.
BMC Infect Dis. 2013 Jan 30;13:55. doi: 10.1186/1471-2334-13-55.

All Publications

2016

Circulation of Dirofilaria repens and Dirofilaria immitis in Moldova.
Șuleșco T, von Thien H, Toderaș L, Toderaș I, Lühken R, Tannich E.
Parasit Vectors. 2016 Dec 3;9(1):627.

Octopamine controls starvation resistance, life span and metabolic traits in Drosophila.
Li Y, Hoffmann J, Li Y, Stephano F, Bruchhaus I, Fink C, Roeder T.
Sci Rep. 2016 Oct 19;6:35359.

Overexpression of Differentially Expressed Genes Identified in Non-pathogenic and Pathogenic Entamoeba histolytica Clones Allow Identification of New Pathogenicity Factors Involved in Amoebic Liver Abscess Formation.
Meyer M, Fehling H, Matthiesen J, Lorenzen S, Schuldt K, Bernin H, Zaruba M, Lender C, Ernst T, Ittrich H, Roeder T, Tannich E, Lotter H, Bruchhaus I.
PLoS Pathog. 2016 Aug 30;12(8):e1005853. doi: 10.1371/journal.ppat.1005853.

Diagnostic performance of the Luminex xTAG gastrointestinal pathogens panel to detect rotavirus in Ghanaian children with and without diarrhoea.
Leva A, Eibach D, Krumkamp R, Käsmaier J, Rubbenstroth D, Adu-Sarkodie Y, May J, Tannich E, Panning M.
Virol J. 2016 Jul 29;13:132. doi: 10.1186/s12985-016-0588-1.

Sharp increase of imported Plasmodium vivax malaria seen in migrants from Eritrea in Hamburg, Germany.
Roggelin L, Tappe D, Noack B, Addo MM, Tannich E, Rothe C.
Malar J. 2016 Jun 17;15:325. doi: 10.1186/s12936-016-1366-7.

Host-feeding patterns of mosquito species in Germany.
Börstler J, Jöst H, Garms R, Krüger A, Tannich E, Becker N, Schmidt-Chanasit J, Lühken R.
Parasit Vectors. 2016 Jun 3;9(1):318. doi: 10.1186/s13071-016-1597-z.

Sporozoite Route of Infection Influences In Vitro var Gene Transcription of Plasmodium falciparum Parasites From Controlled Human Infections
Dimonte S, Bruske EI, Hass J, Supan C, Salazar CL, Held J, Tschan S, Esen M, Flötenmeyer M, Koch I, Berger J, Bachmann A, Sim BK, Hoffman SL, Kremsner PG, Mordmüller B, Frank M.
J Infect Dis. 2016 Sep 15;214(6):884-94. doi: 10.1093/infdis/jiw225.

Culex pipiens and Culex torrentium populations from Germany are susceptible to West Nile virus infection.
Leggewie M, Badusche M, Rudolf M, Jansen S, Börstker J, Huber K, Krüger A, Schmidt-Chanasit J, Tannich E, Becker SC.
One Health (2016) 2, 88-94

Detection of Dirofilaria repens and Dirofilaria immitis DNA in mosquitoes from Belarus.
Șuleșco T, Volkova T, Yashkova S, Tomazatos A, von Thien H, Lühken R, Tannich E.
Parasitol Res. 2016 Sep;115(9):3535-41. doi: 10.1007/s00436-016-5118-y.

Social stress increases the susceptibility to infection in the ant Harpegnathos saltator.
Schneider SA, Scharffetter C, Wagner AE, Boesch C, Bruchhaus I, Rimbach G, Roeder T.
Sci Rep. 2016 May 10;6:25800. doi: 10.1038/srep25800.

Plasmodium falciparum STEVOR phosphorylation regulates host erythrocyte deformability enabling malaria parasite transmission.
Naissant B, Dupuy F, Duffier Y, Lorthiois A, Duez J, Scholz J, Buffet P, Merckx A, Bachmann A, Lavazec C.
Blood. 2016 Jun 16;127(24):e42-53. doi: 10.1182/blood-2016-01-690776.

Application of a multiplex PCR assay for the detection of gastrointestinal pathogens in a rural African setting.
Eibach D, Krumkamp R, Hahn A, Sarpong N, Adu-Sarkodie Y, Leva A, Käsmaier J, Panning M, May J, Tannich E.
BMC Infect Dis. 2016 Apr 14;16(1):150. doi: 10.1186/s12879-016-1481-7.

Mosquito Passage Dramatically Changes var Gene Expression in Controlled Human Plasmodium falciparum Infections.
Bachmann A, Petter M, Krumkamp R, Esen M, Held J, Scholz JA, Li T, Sim BK, Hoffman SL, Kremsner PG, Mordmüller B, Duffy MF, Tannich E.
PLoS Pathog. 2016 Apr 12;12(4):e1005538. doi: 10.1371/journal.ppat.1005538.

Phylogeny of haemosporidian blood parasites revealed by a multi-gene approach.
Borner J, Pick C, Thiede J, Kolawole OM, Kingsley MT, Schulze J, Cottontail VM, Wellinghausen N, Schmidt-Chanasit J, Bruchhaus I, Burmester T.
Mol Phylogenet Evol. 2016 Jan;94(Pt A):221-31. doi: 10.1016/j.ympev.2015.09.003.

The cytokine profile of human NKT cells and PBMCs is dependent on donor sex and glycolipid ligands used for stimulation.
Bernin H, Fehling H, Marggraff C, Tannich E, Lotter H.
Med Microbiol Immunol. 2016 Aug;205(4):321-32. doi: 10.1007/s00430-016-0449-y.

Distribution of individual members of the mosquito Anopheles maculipennis complex in Germany identified by newly developed real-time PCR assays.
Lühken R, Czajka C, Steinke S, Jöst H, Schmidt-Chanasit J, Pfitzner W, Becker N, Kiel E, Krüger A, Tannich E.
Med Vet Entomol. 2016 Jun;30(2):144-54. doi: 10.1111/mve.12161.

IL-23 promotes immunopathology and prevents IL-13-dependent tissue repair associated with Ly6Clo monocytes in Entamoeba histolytica-induced liver damage.
Noll J, Helk E, Fehling H, Bernin H, Marggraff C, Jacobs T, Huber S, Pelczar P, Ernst T, Ittrich H, Otto B, Mittrücker HW, Hölscher C, Tacke F, Bruchhaus I, Tannich E, Lotter H.
J Hepatol. 2016 May;64(5):1147-57. doi: 10.1016/j.jhep.2016.01.013.

Rapid-antigen test negative malaria in a traveller returning from Thailand, molecularly diagnosed as Plasmodium knowlesi
Mackroth MS, Tappe D, Tannich E, Addo M, Rothe C.
Open Forum Infect Dis. 2016 Mar 21;3(1):ofw039. doi: 10.1093/ofid/ofw039.

Tropheryma whipplei in children with diarrhoea in rural Ghana.
Vinnemeier CD, Klupp EM, Krumkamp R, Rolling T, Fischer N, Owusu-Dabo E, Addo MM, Adu-Sarkodie Y, Käsmaier J, Aepfelbacher M, Cramer JP, May J, Tannich E.
Clin Microbiol Infect. 2016 Jan;22(1):65.e1-3. doi: 10.1016/j.cmi.2015.09.022.

A Somali refugee with fever and abnormal blood smear.
Jochum J, Tannich E, Tappe D, Schmiedel S.
Internist (Berl). 2016 Nov 7. [Epub ahead of print] German.

2015

A comparative study of the localization and membrane topology of members of the RIFIN, STEVOR and PfMC-2TM protein families in Plasmodium falciparum-infected erythrocytes.
Bachmann A, Scholz JA, Janßen M, Klinkert MQ, Tannich E, Bruchhaus I, Petter M.
Malar J. 2015 Jul 16;14:274. doi: 10.1186/s12936-015-0784-2.

Human case of Onchocerca lupi infection, Germany, August 2014.
Bergua A, Hohberger B, Held J, Muntau B, Tannich E, Tappe D.
Euro Surveill. 2015 Apr 23;20(16). pii: 21099.

Cysteine peptidases in pathogenesis.
Bruchhaus I, Matthiesen J.
(2015) Amebiasis. Biology and Pathogenesis of Entamoeba. Tomoyoshi Nozaki and Alok Bhattacharya (Eds.), Springer, ISBN 978-4-431-55199-7.

Molecular characterization of Cryptosporidium spp. among children in rural Ghana.
Eibach D, Krumkamp R, Al-Emran HM, Sarpong N, Hagen RM, Adu-Sarkodie Y, Tannich E, May J.
PLoS Negl Trop Dis. 2015 Mar 6;9(3):e0003551. doi: 10.1371/journal.pntd.0003551.

Magnetic resonance imaging of pathogenic protozoan parasite Entamoeba histolytica labeled with superparamagnetic iron oxide nanoparticles.
Ernst TM, Fehling H, Bernin H, Zaruba MD, Bruchhaus I, Adam G, Ittrich H, Lotter H.
Invest Radiol. 2015 Oct;50(10):709-18. doi: 10.1097/RLI.0000000000000175.

Outbreak of Cryptosporidium hominis following river flooding in the city of Halle (Saale), Germany, August 2013.
Gertler M, Dürr M, Renner P, Poppert S, Askar M, Breidenbach J, Frank C, Preußel K, Schielke A, Werber D, Chalmers R, Robinson G, Feuerpfeil I, Tannich E, Gröger C, Stark K, Wilking H.
BMC Infect Dis. 2015 Feb 22;15:88. doi: 10.1186/s12879-015-0807-1.

Comparison of two real-time PCR assays for the detection of malaria parasites from hemolytic blood samples - Short communication.
Hagen RM, Hinz R, Tannich E, Frickmann H.
Eur J Microbiol Immunol (Bp). 2015 Jun 18;5(2):159-63. doi: 10.1556/1886.2015.00006.

Host immunity and tissue destruction during amebic liver abscess formation.
Helk E, Bernin H, Lotter H.
(2015) Amebiasis. Biology and Pathogenesis of Entamoeba. Tomoyoshi Nozaki and Alok Bhattacharya (Eds.), Springer, ISBN 978-4-431-55199-7.

Sex differences in H7N9 influenza A virus pathogenesis.
Hoffmann J, Otte A, Thiele S, Lotter H, Shu Y, Gabriel G.
Vaccine. 2015 Dec 8;33(49):6949-54. doi: 10.1016/j.vaccine.2015.08.044.

Gastrointestinal infections and diarrheal disease in Ghanaian infants and children: an outpatient case-control study.
Krumkamp R, Sarpong N, Schwarz NG, Adlkofer J, Loag W, Eibach D, Hagen RM, Adu-Sarkodie Y, Tannich E, May J.
PLoS Negl Trop Dis. 2015 Mar 4;9(3):e0003568. doi: 10.1371/journal.pntd.0003568.

Physico-Chemical Characteristics of Culex pipiens sensu lato and Culex torrentium (Diptera: Culicidae) Breeding Sites in Germany.
Lühken R, Steinke S, Leggewie M, Tannich E, Krüger A, Becker S, Kiel E.
J Med Entomol. 2015 Sep;52(5):932-6. doi: 10.1093/jme/tjv070.

Fatal Human Meningoencephalitis due to Halicephalobus Nematodes, Germany.
Monoranu CM, Müllges W, Keppler M, Brehm K, Ondrejka SL, Muntau B, Tannich E, Müller-Hermelink HK, Tappe D.
Open Forum Infect Dis. 2015 May 22;2(2):ofv061. doi: 10.1093/ofid/ofv061.

The Plasmodium falciparum exportome contains non-canonical PEXEL/HT proteins.
Schulze J, Kwiatkowski M, Borner J, Schlüter H, Bruchhaus I, Burmester T, Spielmann T, Pick C.
Mol Microbiol. 2015 Jul;97(2):301-14. doi: 10.1111/mmi.13024.

Type of in vitro cultivation influences cytoadhesion, knob structure, protein localization and transcriptome profile of Plasmodium falciparum.
Tilly AK, Thiede J, Metwally N, Lubiana P, Bachmann A, Roeder T, Rockliffe N, Lorenzen S, Tannich E, Gutsmann T, Bruchhaus I.
Sci Rep. 2015 Nov 16;5:16766. doi: 10.1038/srep16766.

Abteilung Molekulare Parasitologie
2014

Stechmücken als Überträger exotischer Krankheitserreger in Deutschland.
Becker N, Krüger A, Kuhn C, Plenge-Bönig A, Thomas S, Schmidt-Chanasit J, Tannich E.
Bundesgesundheitsblatt (2014) 57, 531-540

Immune markers characteristic for asymptomatically infected and diseased Entamoeba histolytica individuals and their relation to sex.
Bernin H, Marggraff C, Jacobs T, Brattig N, Le VA, Blessmann J, Lotter H.
BMC Infect Dis. 2014 Nov 25;14:621. doi: 10.1186/s12879-014-0621-1.

Sex bias in the outcome of human tropical infectious diseases: influence of steroid hormones.
Bernin H, Lotter H.
J Infect Dis. 2014 Jul 15;209 Suppl 3:S107-13. doi: 10.1093/infdis/jit610. Review.

The cell surface proteome of Entamoeba histolytica
Biller l, Matthiesen J, Kühne V, Lotter H, Handal G, Nozaki T, Saito-Nakano Y, Schürmann M, Roeder T, Tannich E, Krause E, Bruchhaus I.
Mol Cell Protomics. 2014; 13(1):132-144. doi: 10.1074/mcp.M113.031393

The use of morphometric wing characters to discriminate female Culex pipiens and Culex torrentium.
Börstler J, Lühken R, Rudolf M, Steinke S, Melaun C, Becker S, Garms R, Krüger A.
J Vector Ecol. 2014 Jun;39(1):204-12. doi: 10.1111/j.1948-7134.2014.12088.x.

Stable transmission of Dirofilaria repens nematodes, Northern Germany
Czajka C, Becker N, Jöst H, Poppert S, Schmidt-Chanasit J, Krüger A, Tannich E.
Emerg Infect Dis. 2014 20(2):328-330. doi: 10.3201/eid2002.131003

Evidence of promiscuous endothelial binding by Plasmodium falciparum-infected erythrocytes.
Esser C, Bachmann A, Kuhn D, Schuldt K, Förster B, Thiel M, May J, Koch-Nolte F, Yáñez-Mó M, Sánchez-Madrid F, Schinkel AH, Jalkanen S, Craig AG, Bruchhaus I, Horstmann RD.
Cell Microbiol. 2014 May;16(5):701-8. doi: 10.1111/cmi.12270.

Transcriptional regionalization of the fruit fly's airway epithelium.
Faisal MN, Hoffmann J, El-Kholy S, Kallsen K, Wagner C, Bruchhaus I, Fink C, Roeder T.
PLoS One. 2014 Jul 14;9(7):e102534. doi: 10.1371/journal.pone.0102534. eCollection 2014.

Cyclovirus CyCV-VN species distribution is not limited to Vietnam and extends to Africa.
Garigliany MM, Hagen RM, Frickmann H, May J, Schwarz NG, Perse A, Jöst H, Börstler J, Shahhosseini N, Desmecht D, Mbunkah HA, Daniel AM, Kingsley MT, Campos Rde M, de Paula VS, Randriamampionona N, Poppert S, Tannich E, Rakotozandrindrainy R, Cadar D, Schmidt-Chanasit J.
Sci Rep. 2014 Dec 18;4:7552. doi: 10.1038/srep07552.

Diagnosis of neuroschistosomiasis by antibody specificity index and semi-quantitative real-time PCR from cerebrospinal fluid and serum.
Härter G, Frickmann H, Zenk S, Wichmann D, Ammann B, Kern P, Fleischer B, Tannich E, Poppert S.
J Med Microbiol. 2014 Feb;63(Pt 2):309-12. doi: 10.1099/jmm.0.066142-0.

Aedes japonicus japonicus (Diptera: Culicidae) from Germany have vector competence for Japan encephalitis virus but are refractory to infection with West Nile virus.
Huber K, Jansen S, Leggewie M, Badusche M, Schmidt-Chanasit J, Becker N, Tannich E, Becker SC.
Parasitol Res. 2014 Sep;113(9):3195-9. doi: 10.1007/s00436-014-3983-9.

Distribution and genetic structure of Aedes japonicus japonicus populations (Diptera: Culicidae) in Germany.
Huber K, Schuldt K, Rudolf M, Marklewitz M, Fonseca DM, Kaufmann C, Tsuda Y, Junglen S, Krüger A, Becker N, Tannich E, Becker SC.
Parasitol Res. 2014 Sep;113(9):3201-10. doi: 10.1007/s00436-014-4000-z.

Mosquitoes (Diptera: Culicidae) of metropolitan Hamburg, Germany.
Krüger A, Börstler J, Badusche M, Lühken R, Garms R, Tannich E.
Parasitol Res. 2014 Aug;113(8):2907-14. doi: 10.1007/s00436-014-3952-3.

Field evaluation of four widely used mosquito traps in Central Europe.
Lühken R, Pfitzner WP, Börstler J, Garms R, Huber K, Schork N, Steinke S, Kiel E, Becker N, Tannich E, Krüger A.
Parasit Vectors. 2014 Jun 12;7:268. doi: 10.1186/1756-3305-7-268.

Lophophorata monophyletic – after all.
Nesnidal MP, Helmkampf M, Bruchhaus I, Ebersberg I, Hausdorf B.
(2014) In: Deep Metazoan Phylogeny: Backbone of the Tree of Life. Edited by Waegele JW, Bartholomaeus T, Misof B, Vogt L. Berlin: Walter de Gruyter.

Dirofilaria repens and Dirofilaria immitis DNA findings in mosquitoes in Germany: temperature data allow autochthonous extrinsic development.
Sassnau R, Czajka C, Kronefeld M, Werner D, Genchi C, Tannich E, Kampen H.
Parasitol Res. 2014 Aug;113(8):3057-61. doi: 10.1007/s00436-014-3970-1.

A case of autochthonous human Dirofilaria infection, Germany, March 2014.
Tappe D, Plauth M, Bauer T, Muntau B, Dießel L, Tannich E, Herrmann-Trost P.
Euro Surveill. 2014 May 1;19(17):2-4.

2013

Diversity and distribution of Blastocystis sp. subtypes in non-human primates.
Alfellani MA, Jacob AS, Perea NO, Krecek RC, Taner-Mulla D, Verweij JJ, Levecke B, Tannich E, Clark CG, Stensvold CR.
Parasitology. 2013 Jul;140(8):966-71. doi: 10.1017/S0031182013000255.

Repeated introduction of Aedes albopictus into Germany, July to October 2012.
Becker N, Geier M, Balczun C, Bradersen U, Huber K, Kiel E, Krüger A, Lühken R, Orendt C, Plenge-Bönig A, Rose A, Schaub GA, Tannich E.
Parasitol Res. 2013 Apr;112(4):1787-90. doi: 10.1007/s00436-012-3230-1.

Diagnostic issues of acute schistosomiasis with Schistosoma mekongi in a traveler: a case report.
Clerinx J, Cnops L, Huyse T, Tannich E, Van Esbroeck M.
J Travel Med. 2013 Sep-Oct;20(5):322-5. doi: 10.1111/jtm.12040.

Influence of parasite density and sample storage time on the reliability of Entamoeba histolytica-specific PCR from formalin-fixed and paraffin-embedded tissues.
Frickmann H, Tenner-Racz K, Eggert P, Schwarz NG, Poppert S, Tannich E, Hagen RM.
Diagn Mol Pathol. 2013 Dec;22(4):236-44. doi: 10.1097/PDM.0b013e3182936936.

Food and drinking water hygiene and intestinal protozoa in deployed German soldiers.
Frickmann H, Schwarz NG, Wiemer DF, Fischer M, Tannich E, Scheid PL, Müller M, Schotte U, Bock W, Hagen RM.
Eur J Microbiol Immunol (Bp). 2013 Mar;3(1):53-60. doi: 10.1556/EuJMI.3.2013.1.8

TNFα-mediated liver destruction by Kupffer cells and Ly6Chi monocytes during Entamoeba histolytica infection.
Helk E, Bernin H, Ernst T, Ittrich H, Jacobs T, Heeren J, Tacke F, Tannich E, Lotter H.
PLoS Pathog. 2013 Jan;9(1):e1003096. doi: 10.1371/journal.ppat.1003096.

Janitschke K, Kimmig P, Seitz HM, Frosch M, Groß U, Hlobil H, Reiter-Owona I, Bogdan C, Tannich E, Fleischer B, Hörauf A, Tappe D, Walochnik J.
Parasitosen. (2014) In: Qualitätsstandards in der mikrobiologisch-infektiologischen Diagnostik.
Herausgeber: H. Mauch, R. Lütticken und S. Gatermann. Urban & Fischer, Jena

Rediscovery of Anopheles algeriensis Theob. (Diptera: Culicidae) in Germany after half a century.
Krüger A, Tannich E.
Eur Mosq Bulletin (2013) 31, 14-16

Testosterone increases susceptibility to amebic liver abscess in mice and mediates inhibition of IFNγ secretion in natural killer T cells.
Lotter H, Helk E, Bernin H, Jacobs T, Prehn C, Adamski J, González-Roldán N, Holst O, Tannich E.
PLoS One. 2013;8(2):e55694. doi: 10.1371/journal.pone.0055694.

Overexpression of specific cysteine peptidases confers pathogenicity to a nonpathogenic Entamoeba histolytica clone.
Matthiesen J, Bär AK, Bartels AK, Marien D, Ofori S, Biller L, Tannich E, Lotter H, Bruchhaus I.
MBio. 2013 Mar 26;4(2). pii: e00072-13. doi: 10.1128/mBio.00072-13.

Use of multiplex real-time PCR for detection of common diarrhea causing protozoan parasites in Egypt.
Nazeer J, Khalifa M, von Thien H, El-Sibaei M, Abdel-Hamid M, Tawfik R, Tannich E.
Parasitol Res (2013) 112, 595-601

Agent of whirling disease meets orphan worm: phylogenomic analyses firmly place Myxozoa in Cnidaria.
Nesnidal MP, Helmkampf M, Meyer A, Witek A, Bruchhaus I, Ebersberger I, Hankeln T, Lieb B, Struck TH, Hausdorf B.
PLoS One. 2013;8(1):e54576. doi: 10.1371/journal.pone.0054576.

New phylogenomic data support the monophyly of Lophophorata and an Ectoproct-Phoronid clade and indicate that Polyzoa and Kryptrochozoa are caused by systematic bias.
Nesnidal MP, Helmkampf M, Meyer A, Witek A, Bruchhaus I, Ebersberger I, Hankeln T, Lieb B, Struck TH, Hausdorf B.
BMC Evol Biol. 2013 Nov 17;13:253. doi: 10.1186/1471-2148-13-253.

First nationwide surveillance of Culex pipiens complex and Culex torrentium mosquitoes demonstrated the presence of Culex pipiens biotype pipiens/molestus hybrids in Germany.
Rudolf M, Czajka C, Börstler J, Melaun C, Jöst H, von Thien H, Badusche M, Becker N, Schmidt-Chanasit J, Krüger A, Tannich E, Becker S.
PLoS One. 2013 Sep 11;8(9):e71832. doi: 10.1371/journal.pone.0071832. eCollection 2013.

Prospective European-wide multicentre study on a blood based real-time PCR for the diagnosis of acute schistosomiasis.
Wichmann D, Poppert S, Von Thien H, Clerinx J, Dieckmann S, Jensenius M, Parola P, Richter J, Schunk M, Stich A, Zanger P, Burchard GD, Tannich E.
Wichmann D, Poppert S, Von Thien H, Clerinx J, Dieckmann S, Jensenius M, Parola P, Richter J, Schunk M, Stich A, Zanger P, Burchard GD, Tannich E.BMC Infect Dis. 2013 Jan 30;13:55. doi: 10.1186/1471-2334-13-55.

An unusual complication in ulcerative colitis during treatment with azathioprine and infliximab: Isospora belli as 'Casus belli'.
Stein J, Tannich E, Hartmann F.
BMJ Case Rep. 2013 May 24;2013. pii: bcr2013009837. doi: 10.1136/bcr-2013-009837.

Histolyasin and other Entamoeba cysteine endopeptidases
Scholze H, Tannich E, Bruchhaus I.
Handbook of Proteolytic Enzymes, 3rd edition, Rawlings N.D. and Salvesen, G., eds., Ch. 439, Elsevier, pp. 1919-1922. 2013.

Amöbiasis. (2013)
Tannich E, Kern P, Burchard GD.
DGPI Handbuch. Infektionen bei Kindern und Jugendlichen. 6. Auflage. Herausgeber: H. Scholz, B.H. Belohradsky, R. Bialek, U. Heininger, H.W. Kreth, R. Roos. Thieme Verlag, Stuttgart, 150-152

2012

The ecology of mosquitoes in an irrigated vegetable farm in Kumasi, Ghana: abundance, productivity and survivorship.
Afrane YA, Lawson BW, Brenya R, Kruppa T, Yan G.
Parasit Vectors. 2012 Oct 15;5:233. doi: 10.1186/1756-3305-5-233.

Temporal expression and localization patterns of variant surface antigens in clinical Plasmodium falciparum isolates during erythrocyte schizogony.
Bachmann A, Petter M, Tilly AK, Biller L, Uliczka KA, Duffy MF, Tannich E, Bruchhaus I.
PLoS One. 2012;7(11):e49540. doi: 10.1371/journal.pone.0049540.

Epizootic emergence of Usutu virus in wild and captive birds in Germany.
Becker N, Jöst H, Ziegler U, Eiden M, Höper D, Emmerich P, Fichet-Calvet E, Ehichioya DU, Czajka C, Gabriel M, Hoffmann B, Beer M, Tenner-Racz K, Racz P, Günther S, Wink M, Bosch S, Konrad A, Pfeffer M, Groschup MH, Schmidt-Chanasit J.
PLoS One. 2012;7(2):e32604. doi: 10.1371/journal.pone.0032604.

Antepartum depression and anxiety associated with disability in African women: cross-sectional results from the CDS study in Ghana and Côte d'Ivoire.
Bindt C, Appiah-Poku J, Te Bonle M, Schoppen S, Feldt T, Barkmann C, Koffi M, Baum J, Nguah SB, Tagbor H, Guo N, N'Goran E, Ehrhardt S; International CDS Study Group.
PLoS One. 2012;7(10):e48396. doi: 10.1371/journal.pone.0048396

Multisite performance evaluation of an enzyme-linked immunosorbent assay for detection of Giardia, Cryptosporidium, and Entamoeba histolytica antigens in human stool.
Christy NC, Hencke JD, Escueta-De Cadiz A, Nazib F, von Thien H, Yagita K, Ligaba S, Haque R, Nozaki T, Tannich E, Herbein JF, Petri WA Jr.
J Clin Microbiol. 2012 May;50(5):1762-3. doi: 10.1128/JCM.06483-11.

Schistosoma real-time PCR as diagnostic tool for international travellers and migrants.
Cnops L, Tannich E, Polman K, Clerinx J, Van Esbroeck M.
Trop Med Int Health. 2012 Oct;17(10):1208-16. doi: 10.1111/j.1365-3156.2012.03060.x.

Molecular detection of Setaria tundra (Nematoda: Filarioidea) and an unidentified filarial species in mosquitoes in Germany.
Czajka C, Becker N, Poppert S, Jöst H, Schmidt-Chanasit J, Krüger A.
Parasit Vectors. 2012 Jan 11;5:14. doi: 10.1186/1756-3305-5-14.

Bats host major mammalian paramyxoviruses.
Drexler JF, Corman VM, Müller MA, Maganga GD, Vallo P, Binger T, Gloza-Rausch F, Cottontail VM, Rasche A, Yordanov S, Seebens A, Knörnschild M, Oppong S, Adu Sarkodie Y, Pongombo C, Lukashev AN, Schmidt-Chanasit J, Stöcker A, Carneiro AJ, Erbar S, Maisner A, Fronhoffs F, Buettner R, Kalko EK, Kruppa T, Franke CR, Kallies R, Yandoko ER, Herrler G, Reusken C, Hassanin A, Krüger DH, Matthee S, Ulrich RG, Leroy EM, Drosten C.
Nat Commun. 2012 Apr 24;3:796. doi: 10.1038/ncomms1796.

Human parvovirus 4 in nasal and fecal specimens from children, Ghana.
Drexler JF, Reber U, Muth D, Herzog P, Annan A, Ebach F, Sarpong N, Acquah S, Adlkofer J, Adu-Sarkodie Y, Panning M, Tannich E, May J, Drosten C, Eis-Hübinger AM.
Emerg Infect Dis. 2012 Oct;18(10):1650-3. doi: 10.3201/eid1810.111373.

Evaluation of fluorescence in situ hybridisation (FISH) for the identification of Candida albicans in comparison with three phenotypic methods.
Lakner A, Essig A, Frickmann H, Poppert S.
Mycoses. 2012 May;55(3):e114-23. doi: 10.1111/j.1439-0507.2011.02154.x.

A novel Entamoeba histolytica inositol phosphate kinase catalyzes the formation of 5PP-Ins(1,2,3,4,6)P(5).
Löser B1, Nalaskowski MM, Fanick W, Lin H, Tannich E, Mayr GW.
Mol Biochem Parasitol. 2012 Jan;181(1):49-52. doi: 10.1016/j.molbiopara.2011.09.008.

The effect of deltamethrin-treated net fencing around cattle enclosures on outdoor-biting mosquitoes in Kumasi, Ghana.
Maia MF, Abonuusum A, Lorenz LM, Clausen PH, Bauer B, Garms R, Kruppa T.
PLoS One. 2012;7(9):e45794. doi: 10.1371/journal.pone.0045794.

Amoebic liver abscess with negative serologic markers for Entamoeba histolytica: mind the gap!
Marn H, Ignatius R, Tannich E, Harms G, Schürmann M, Dieckmann S.
Infection. 2012 Feb;40(1):87-91. doi: 10.1007/s15010-011-0157-x.

The cysteine protease inhibitors EhICP1 and EhICP2 perform different tasks in the regulation of endogenous protease activity in trophozoites of Entamoeba histolytica.
Šarić M, Irmer H, Eckert D, Bär AK, Bruchhaus I, Scholze H.
Protist. 2012 Jan;163(1):116-28. doi: 10.1016/j.protis.2011.01.003.

Entamoeba cysteine endopeptidases belonging to the C1 papain superfamily.
Scholze H, Tannich E, Bruchhaus I.
Handbook of Proteolytic Enzymes, 3nd edition, Rawlings N.D. and Salvesen, G., eds., Ch. 438, Elsevier, (2012) pp. 1915-1918.

Entamoeba histolytica.
Tannich E.
Lexikon der Infektionskrankheiten des Menschen. Erreger, Symptome, Diagnose, Therapie und Prophylaxe. 4. Auflage. Herausgeber: G. Darai, M. Handermann, H.-G. Sonntag, C.A. Tidona, L. Zöller. Springer Verlag, Heidelberg, (2012) 270-274

Genomic diversity of the human intestinal parasite Entamoeba histolytica.
Weedall GD, Clark CG, Koldkjaer P, Kay S, Bruchhaus I, Tannich E, Paterson S, Hall N.
Genome Biol. 2012 May 25;13(5):R38. doi: 10.1186/gb-2012-13-5-r38.

Rapid identification of the animal pathogens Streptococcus uberis and Arcanobacterium pyogenes by fluorescence in situ hybridization (FISH).
Werckenthin C, Gey A, Straubinger RK, Poppert S.
Vet Microbiol. 2012 May 4;156(3-4):330-5. doi: 10.1016/j.vetmic.2011.10.007.

2011

Malaria transmission in two rural communities in the forest zone of Ghana.
Abonuusum A1, Owusu-Daako K, Tannich E, May J, Garms R, Kruppa T.
Parasitol Res. 2011 Jun;108(6):1465-71. doi: 10.1007/s00436-010-2195-1.

Highly co-ordinated var gene expression and switching in clinical Plasmodium falciparum isolates from non-immune malaria patients.
Bachmann A, Predehl S, May J, Harder S, Burchard GD, Gilberger TW, Tannich E, Bruchhaus I.
Cell Microbiol. 2011 Sep;13(9):1397-409. doi: 10.1111/j.1462-5822.2011.01629.x.

Acute schistosomiasis in a cluster of travelers from Rwanda: diagnostic contribution of schistosome DNA detection in serum compared to parasitology and serology.
Clerinx J, Bottieau E, Wichmann D, Tannich E, Van Esbroeck M.
J Travel Med. 2011 Nov-Dec;18(6):367-72. doi: 10.1111/j.1708-8305.2011.00552.x.

Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by fluorescence in situ hybridization (FISH) from culture and paraffin-embedded tissue samples.
Hagen RM1, Frickmann H, Elschner M, Melzer F, Neubauer H, Gauthier YP, Racz P, Poppert S.
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Novel real-time PCR for the universal detection of Strongyloides species.
Kramme S, Nissen N, Soblik H, Erttmann K, Tannich E, Fleischer B, Panning M, Brattig N.
J Med Microbiol. 2011 Apr;60(Pt 4):454-8. doi: 10.1099/jmm.0.025338-0.

The complete mitochondrial genome of Flustra foliacea (Ectoprocta, Cheilostomata) - compositional bias affects phylogenetic analyses of lophotrochozoan relationships.
Nesnidal MP, Helmkampf M, Bruchhaus I, Hausdorf B.
BMC Genomics. 2011 Nov 23;12:572. doi: 10.1186/1471-2164-12-572.

Comparative evaluation of two rapid field tests for malaria diagnosis: Partec Rapid Malaria Test® and Binax Now® Malaria Rapid Diagnostic Test.
Nkrumah B, Acquah SE, Ibrahim L, May J, Brattig N, Tannich E, Nguah SB, Adu-Sarkodie Y, Huenger F.
BMC Infect Dis. 2011 May 23;11:143. doi: 10.1186/1471-2334-11-143.

Phylogenomic analyses of malaria parasites and evolution of their exported proteins.
Pick C, Ebersberger I, Spielmann T, Bruchhaus I, Burmester T.
BMC Evol Biol. 2011 Jun 15;11:167. doi: 10.1186/1471-2148-11-167.

Increased sampling reveals novel lineages of Entamoeba: consequences of genetic diversity and host specificity for taxonomy and molecular detection.
Stensvold CR, Lebbad M, Victory EL, Verweij JJ, Tannich E, Alfellani M, Legarraga P, Clark CG.
Protist. 2011 Jul;162(3):525-41. doi: 10.1016/j.protis.2010.11.002.

Real-time multiplex PCR for simultaneous detection of Campylobacter jejuni, Salmonella, Shigella and Yersinia species in fecal samples.
Wiemer D, Loderstaedt U, von Wulffen H, Priesnitz S, Fischer M, Tannich E, Hagen RM.
Int J Med Microbiol. 2011 Nov;301(7):577-84. doi: 10.1016/j.ijmm.2011.06.001.

2010

Differences in the transcriptome signatures of two genetically related Entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties.
Biller L, Davis PH, Tillack M, Matthiesen J, Lotter H, Stanley SL Jr, Tannich E, Bruchhaus I.
BMC Genomics. 2010 Jan 26;11:63. doi: 10.1186/1471-2164-11-63.

Labordiagnostik.
Bretzel G, Tannich E.
Tropenmedizin in Klinik und Praxis. Herausgeber T. Löscher und G.-D. Burchard. Thieme Verlag, Stuttgart, (2010) 994-1019.

The essentials of protein import in the degenerate mitochondrion of Entamoeba histolytica.
Dolezal P, Dagley MJ, Kono M, Wolynec P, Likić VA, Foo JH, Sedinová M, Tachezy J, Bachmann A, Bruchhaus I, Lithgow T.
PLoS Pathog. 2010 Mar 19;6(3):e1000812. doi: 10.1371/journal.ppat.1000812.

Characterization of a subunit of the outer dynein arm docking complex necessary for correct flagellar assembly in Leishmania donovani.
Harder S, Thiel M, Clos J, Bruchhaus I.
PLoS Negl Trop Dis. 2010 Jan 26;4(1):e586. doi: 10.1371/journal.pntd.0000586.

Phylogenetic relationships within the lophophorate lineages (Ectoprocta, Brachiopoda and Phoronida).
Hausdorf B, Helmkampf M, Nesnidal MP, Bruchhaus I.
Mol Phylogenet Evol. 2010 Jun;55(3):1121-7. doi: 10.1016/j.ympev.2009.12.022.

tRNA gene sequences are required for transcriptional silencing in Entamoeba histolytica.
Irmer H, Hennings I, Bruchhaus I, Tannich E.
Eukaryot Cell. 2010 Feb;9(2):306-14. doi: 10.1128/EC.00248-09.

Compositional heterogeneity and phylogenomic inference of metazoan relationships.
Nesnidal MP, Helmkampf M, Bruchhaus I, Hausdorf B.
Mol Biol Evol. 2010 Sep;27(9):2095-104. doi: 10.1093/molbev/msq097.

Comparison of the novel Partec rapid malaria test to the conventional Giemsa stain and the gold standard real-time PCR.
Nkrumah B, Agyekum A, Acquah SE, May J, Tannich E, Brattig N, Nguah SB, von Thien H, Adu-Sarkodie Y, Huenger F.
J Clin Microbiol. 2010 Aug;48(8):2925-8. doi: 10.1128/JCM.02403-09.

Rapid identification of Propionibacterium acnes from blood cultures by fluorescence in situ hybridization.
Poppert S, Riecker M, Essig A.
Diagn Microbiol Infect Dis. 2010 Feb;66(2):214-6. doi: 10.1016/j.diagmicrobio.2009.09.007.

Accelerated identification of Staphylococcus aureus from blood cultures by a modified fluorescence in situ hybridization procedure.
Poppert S, Riecker M, Wellinghausen N, Frickmann H, Essig A.
J Med Microbiol. 2010 Jan;59(Pt 1):65-8. doi: 10.1099/jmm.0.015503-0.

Caenopores are antimicrobial peptides in the nematode Caenorhabditis elegans instrumental in nutrition and immunity.
Roeder T, Stanisak M, Gelhaus C, Bruchhaus I, Grötzinger J, Leippe M.
Dev Comp Immunol. 2010 Feb;34(2):203-9. doi: 10.1016/j.dci.2009.09.010.

Amöbiasis und andere Amöbeninfektionen.
Tannich E, Burchard GD.
Tropenmedizin in Klinik und Praxis. Herausgeber T. Löscher und G.-D. Burchard. Thieme Verlag, Stuttgart, 639-650.

Microscopic diagnosis of sodium acetate-acetic acid-formalin-fixed stool samples for helminths and intestinal protozoa: a comparison among European reference laboratories.
Utzinger J, Botero-Kleiven S, Castelli F, Chiodini PL, Edwards H, Köhler N, Gulletta M, Lebbad M, Manser M, Matthys B, N'Goran EK, Tannich E, Vounatsou P, Marti H.
Clin Microbiol Infect. 2010 Mar;16(3):267-73. doi: 10.1111/j.1469-0691.2009.02782.x.

2009

Absence of erythrocyte sequestration and lack of multicopy gene family expression in Plasmodium falciparum from a splenectomized malaria patient.
Bachmann A, Esser C, Petter M, Predehl S, von Kalckreuth V, Schmiedel S, Bruchhaus I, Tannich E.
PLoS One. 2009 Oct 14;4(10):e7459. doi: 10.1371/journal.pone.0007459.

Comparison of two genetically related Entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties.
Biller L, Schmidt H, Krause E, Gelhaus C, Matthiesen J, Handal G, Lotter H, Janssen O, Tannich E, Bruchhaus I.
Proteomics. 2009 Sep;9(17):4107-20. doi: 10.1002/pmic.200900022.

Evaluation of new selective culture media and a rapid fluorescence in situ hybridization assay for identification of Clostridium difficile from stool samples.
Bloedt K, Riecker M, Poppert S, Wellinghausen N.
J Med Microbiol. 2009 Jul;58(Pt 7):874-7. doi: 10.1099/jmm.0.009811-0.

Major cysteine peptidases of Entamoeba histolytica are required for aggregation and digestion of erythrocytes but are dispensable for phagocytosis and cytopathogenicity
Irmer H, Tillack M, Biller L, Handal G, Leippe M, Roeder T, Tannich E, Bruchhaus I.
Mol Microbiol. 2009 May;72(3):658-67. doi: 10.1111/j.1365-2958.2009.06672.x.

Orientia tsutsugamushi bacteremia and cytokine levels in Vietnamese scrub typhus patients.
Kramme S, An le V, Khoa ND, Trin le V, Tannich E, Rybniker J, Fleischer B, Drosten C, Panning M.
J Clin Microbiol. 2009 Mar;47(3):586-9. doi: 10.1128/JCM.00997-08.

Amöbiasis
Kern P, Burchard GD, Tannich E.
DGPI Handbuch. Infektionen bei Kindern und Jugendlichen. 5. Auflage. Herausgeber: H. Scholz, B.H. Belohradsky, R. Bialek, U. Heininger, H.W. Kreth, R. Roos. Thieme Verlag, Stuttgart, (2009) 145-147

Natural killer T cells activated by a lipopeptidophosphoglycan from Entamoeba histolytica are critically important to control amebic liver abscess.
Lotter H, González-Roldán N, Lindner B, Winau F, Isibasi A, Moreno-Lafont M, Ulmer AJ, Holst O, Tannich E, Jacobs T.
PLoS Pathog. 2009 May;5(5):e1000434. doi: 10.1371/journal.ppat.1000434.

Dirofilaria repens infection and concomitant meningoencephalitis.
Poppert S, Hodapp M, Krueger A, Hegasy G, Niesen WD, Kern WV, Tannich E.
Emerg Infect Dis. 2009 Nov;15(11):1844-6. doi: 10.3201/eid1511.090936.

Rapid identification of beta-hemolytic streptococci by fluorescence in situ hybridization (FISH).
Poppert S, Nickel D, Berger A, Yildiz T, Kaestner N, Mauerer S, Spellerberg B.
Int J Med Microbiol. 2009 Aug;299(6):421-6. doi: 10.1016/j.ijmm.2009.02.001.

Amöben.
Tannich E.
Mikrobiologische Diagnostik. 2. Auflage. Herausgeber: B. Neumeister, R.W. Braun, P. Kimmig, H.K. Geiss. Thieme Verlag, Stuttgart, (2009) 978-984

Contact

Prof. Dr. Egbert Tannich

Phone: +49 40 42818-270
Fax: +49 40 42818-265
E-Mail: tannich@bnitm.de


Secretary

Wiebke Böhm (-270)

Support Staff

Claudia Marggraff (-476)
Susann Ofori (-397)
Heidrun von Thien (-476)

Associated Scientists

Prof. Dr. Rolf Garms