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We investigate how arboviruses interact with their mosquito vectors and how these interactions affect virus evolution and transmissibility. We are studying the biotic and abiotic factors of arbovirus emergence and are involved in arbovirus outbreak response. In addition, we provide specific support for outbreak response to the WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research including on-the-spot investigations, confirmation of diagnosis, handling of dangerous (biosafety level III) pathogens, case detection, and provision of logistics in the form of staff and supplies.
Currently, we are performing arbovirus research worldwide with projects in nine areas:
1. The German arbovirus surveillance and mosquito monitoring program
2. Surveillance of emerging arboviruses in Rio de Janeiro, Brazil
3. Vector range, genetic traits and phylogeography of arboviruses in Danube Delta, Romania
4. Metagenomics profile and evolution of the viral communities in field-collected sand flies, mosquitoes and ticks from different remote ecosystems of the Amazonian rainforest, French Guiana
5. Virome of ticks collected from domestic animals in districts of the Tigray Province, Ethiopia
6. Sandfly-borne viruses from Israel and their impact on human and animal health
7. Domestication of Aedes aegypti and the effect on virus diversity and distribution based on a field study in Benin, West Africa
8. Establishment of an “Early Warning System for Mosquito-borne Diseases” and implementation of new vector control tools in the urban environment of Greece and Germany
9. Vector competence of indigenous mosquitoes from Germany
Human and animal diseases caused by arthropod-borne viruses (arboviruses) are of growing importance in many countries of Europe including Germany (Schmidt-Chanasit et al 2012). Continuing eco-climatic changes and globalization create suitable conditions for the emergence of arboviruses in Germany (Becker et al 2015). Thus, we initiated a program that compiles and analyses arbovirus and vector data collected over a number of successive years. This provided a solid base to determine the underlying causes of the seasonal fluctuations in arbovirus activity and arbovirus emergence (Jöst et al. 2010 and 2011, Becker et al. 2012, Garigliany et al. 2015, Engel et al. 2016, Cadar et al. 2017, Lühken et al. 2017, Heitmann et al. 2018). In addition, the fundamental understanding of the mosquito vector ecology (Börstler et al 2016, Lühken et al. 2016, Leggewie et al. 2018) is the sine qua non to develop and implement sustainable vector and arbovirus control programs in Germany.
In 2013 Germany launched a new program to implement longterm biosecurity projects under Global Partnership auspices: German Partnership Program for Excellence in Biological and Health Security. The program was intended to help partner countries tackle biosecurity threats (e.g. bioterrorism, outbreaks of highly pathogenic diseases, pandemics). The aim was not only to prevent any misuse of infectious agents that could pose a threat not merely to Germany but also to strengthen the health services of Brazil, thus enhancing Brazil´s national security. Under this Program a range of projects were implemented. In cooperation with various specialised Brazilian institutes (Federal University of Rio de Janeiro, Laboratório Central de Saúde Pública do Rio de Janeiro Noel Nutels, Oswaldo Cruz Foundation) we established an early warning system for arbovirus emergence that led to the discovery of Zika virus in Rio de Janeiro and the better understanding of Zika virus ecology (Campos et al. 2016 and 2017, Heitmann et al. 2017), laboratory diagnostics (Huzly et al. 2016, Müller et al. 2016, Steinhagen et al. 2016, Corman et al. 2016, Müller et al. 2017, Rönnberg et al. 2017, Rockstroh et al. 2017) and pathogenesis (Tappe et al. 2016, Gabriel et al. 2017, Röcker et al. 2018, Stanelle-Bertram et al. 2018). Thus, we implemented early warning systems for arbovirus emergence in Rio de Janeiro (Ayllon et al. 2017), reduced arbovirus spread and rapidly identified and eliminated the vectors responsible.
The Danube Delta is the largest continuous marshland and the second largest and best preserved river delta in Europe. The Danube Delta also represents the place with the highest biodiversity and largest wetland in Europe and provides perfect environmental conditions necessary for a diverse and abundant mosquitoes fauna. Being located along major migratory bird routes, the Danube Delta represents an extremely important nesting and feeding zone for a large number of resident and migratory birds from Africa and Asia. Arboviruses are characterized as being mostly local and focal with transmission foci and hotspots which are influenced by the presence of the aquatic biotopes suitable for the development of arthropod populations in the areas where blood feeding occurs. Wild birds are important to public health, because they can act as biological or mechanical carriers of emerging arboviruses, either as a reservoir host or by dispersing infected arthropod vectors. Given that the introduction and spread of the arboviruses into new geographic areas depends on the presence of infected hosts, suitable arthropod vector and favorable habitats, the knowledge of the resident arthropod species population (Török et al 2016), distribution, dynamics, their feeding preferences and vector competence was studied to understand the enzootic cycles (Tomazatos et al. 2018). In addition, this longitudinal arthropod surveillance project helped us to learn more about abovirus ecology and their genetic and phylogeographic characterization.
Different arthropod species (sand flies, mosquitoes and ticks) are vectors of a wide array of arboviruses (arthropod-borne viruses) and have likely been central to viral evolution. To better understand the extent of arthropod-borne pathogens as well as their origin and evolutionary history, it is crucial to uncover the full range of microbial agents, including viruses associated with arthropods. Our current knowledge of the diversity of sand flies-, mosquito- and tick-associated viruses is very scarce, in part due to the lack of investigation of arthropod viromes, at least in remote areas. The majority of the arboviruses sampled to date predominantly has a relationship with vertebrates or are known to be agents of diseases. Recent studies of arthropod RNA viruses have revealed that changes in genome size, structure and segmentation have occurred more frequently and on a larger scale than previously thought, with some arthropod viruses likely to be ancestors of those that infect vertebrates. Anthropogenic and natural changes in the environment and climate changes may in future bring humans closer to microorganisms they have not been previously in contact. The vast majority of viruses are thought to be undiscovered, and recent studies have shown that viruses discovered in insects are very divergent from known pathogens. This project will apply beside the entomological and ecological investigations a so called vector-enabled metagenomics approach on diverse arthropod vector species from different ecosystems of the Amazonian rainforest in French Guiana. It will also provide an unprecedented view of the virome diversity in the insect vectors from different remote and closed ecological habitats as well as its seasonal variability. The characterization of the novel viruses identified by high-throughput sequencing will improve our knowledge on the molecular mechanisms of virus-host interactions in arthropod vectors, and will bring a new perspective on fundamental questions of virus origins, diversity, host range, genome evolution, and disease emergence.
Like in many developing countries, domestic animals play a crucial role in Ethiopia. They provide food in the form of meat and milk. Cattle are a source of cash income through sales of milk, meat and other related products. Furthermore, they act as storage of wealth and determine social status within the community. Despite the large number of cattle in the country, the sector is characterized by low productivity and, hence, income derived from this sector could not impart significant role in the development of Ethiopia´s economy. The low productivity is attributed to high disease incidence and parasite burden. Vector-borne diseases are major constraints to the development of viable livestock industries wherever they occur. Among these, tick and tick-borne diseases are widely distributed in Ethiopia, causing a tremendous economic importance in livestock production. Arboviruses could be responsible for diseases with serious health implications, welfare and socio-economic impact. The present study will focus on the known and novel arboviruses and their genetic characterization from ticks of cattle from Ethiopia. This will help to provide useful information to the concerned stake holders and to develop appropriate and sustainable control and prevention methods.
The general aim of this collaborative effort is focused on the characterization of Phlebotomine sandfly- borne viruses that infect humans in Israel and elucidating their medical importance. Specific objectives will be: (i) Set up country wide surveillance for Phlebotomus and Sergentomyia sandflies fitted to virus detection and blood meal analysis; (ii) Perform molecular and phylogenetic analysis of novel viruses identified in Phlebotomine sandflies in Israel and (iii) characterize the functional and clinical relevance of the identified sandfly-borne viruses.
As there are difficulties in pointing out specific genes that lead to increased vector competence, we conceived that vector competence is more likely the result of the effects of genetic adaptations for other functions being not linked directly with vector competence. Therefore, we will gain new insights in how the adaption to the human environment (for example host-feeding preference, oviposition or resting behavior) influences vector behavior and if there are consequences on virus prevalence. This will be accomplished within a field study in Benin. Ae. aegypti will be trapped along a transect reaching from urban sites with high anthropogenic impact, over rural sites with medium anthrophogenic impact to sylvatic sites with low anthropogenic impact. Changes in the basic transmission parameters of mosquitoes (e.g. host-feeding preference) associated with domestication are particularly important but poorly understood. The link between habitat change and adaptions of the vector and associated pathogens are essential to understand the emergence of viruses transmitted between humans and wildlife.
It was demonstrated that there are differences of virus prevalence in mosquitoes between disturbed and
natural habitats. There should also be a focus on viruses associated exclusively with mosquitoes, named mosquito-only viruses, which are unable to replicate in vertebrates and do not represent a health risk for animals and humans. Therefore, we do not focus only on known viruses, which are often described for West Africa (e.g. DENV or YFV). The emergence of ZIKV in Oceania and the Americas demonstrated the importance of an unbiased virus surveillance. Therefore, the study focus on the detection of known and unknown viruses (moboviruses and mosquito-only viruses) silently circulating in sylvatic and urban Ae. aegypti from Benin and their prevalence change according to the degree of domestication of
the vector. We postulate that the adaptations accompanying domestication have side effects that result in a varied prevalence of viruses in Ae. aegypti.
In this project, we will use the entomological, epidemiological, climatological and geographical data from Germany and Greece already available and/or to be produced by our partners, key actors in the field of pathogen detection and vector control in Europe, in order to develop a common Early Warning System for mosquito borne diseases to be implemented and evaluated in both countries. Substantial exchange of know-how and expertise will take place between two major research institutes in the field arbovirology and transfer to two Major mosquito control operators in Europe for operational purposes.
The project's objectives are: 1. Connecting major European actors in arbovirology and mosquito surveillance and control from Germany and Greece 2. Reanalyze the epidemiological data from the severe WNV epidemic in Northern Greece since 2010 and the USUV outbreaks in Germany since 2011 so as to prepare accordingly Germany and Greece against WNV, USUV and other arboviruses. 3. Identify high risk areas and critical time intervals for WNV and USUV circulation following modeling from entomological data and epidemiological data already produced by the partners during the last years and to be further developed for the years 2018 and 2019 (Lühken et al. 2017). 4. Provide good practices, innovative tools and methods for the control of container-breeding mosquitoes in urban settings, with special consideration of Culex pipiens and Aedes albopictus, which are the major European vectors for existing (USUV in Germany and WNV in Greece) and/or threatening (WNV for Germany, dengue, Chikungunya and Zika for both countries) mosquito-borne diseases. 5. Produce, update and make available this information to concerned Public Health Institutions and/or other bodies of both countries, a functional webGIS, in which the users will have the possibility to view, explore, query, and display the periodical risk maps of the Early Warning System for mosquito-borne diseases.
Within the framework of climate impact research, the vector competence of selected indigenous mosquito species for Sindbis, Usutu, Batai, West Nile, chikungunya, and dengue viruses will be determined (Leggewie et al., 2016, Wagner et al., 2018). Thus, as part of the laboratory work in the BSL-3 insectary mosquito infection and transmission rates for several arboviruses will be determined. In addition, in vitro experiments will focus on the adaption of arboviruses to different temperatures. Based on the data obtained, a risk Analysis will be carried out and necessary preventive measures developed.
Autochthonous Crimean–Congo Hemorrhagic Fever in Spain
Negredo A, de la Calle-Prieto F, Palencia-Herrejón E, Mora-Rillo M, Astray-Mochales J, Sánchez-Seco MP, Bermejo Lopez E, Menárguez J, Fernández-Cruz A, Sánchez-Artola B, Keough-Delgado E, Ramírez de Arellano E, Lasala F, Milla J, Fraile JL, Ordobás Gavín M, Martinez de la Gándara A, López Perez L, Diaz-Diaz D, López-García MA, Delgado-Jimenez P, Martín-Quirós A, Trigo E, Figueira JC, Manzanares J, Rodriguez-Baena E, Garcia-Comas L, Rodríguez-Fraga O, García-Arenzana N, Fernández-Díaz MV, Cornejo VM, Emmerich P, Schmidt-Chanasit J, Arribas JR
N Engl J Med. 2017 Jul 13;377(2):154-161. doi: 10.1056/NEJMoa1615162.
Phase 1 Trials of rVSV Ebola Vaccine in Africa and Europe.
Agnandji ST, Huttner A, Zinser ME, Njuguna P, Dahlke C, Fernandes JF, Yerly S, Dayer JA, Kraehling V, Kasonta R, Adegnika AA, Altfeld M, Auderset F, Bache EB, Biedenkopf N, Borregaard S, Brosnahan JS, Burrow R, Combescure C, Desmeules J, Eickmann M, Fehling SK, Finckh A, Goncalves AR, Grobusch MP, Hooper J, Jambrecina A, Kabwende AL, Kaya G, Kimani D, Lell B, Lemaître B, Lohse AW, Massinga-Loembe M, Matthey A, Mordmüller B, Nolting A, Ogwang C, Ramharter M, Schmidt-Chanasit J, Schmiedel S, Silvera P, Stahl FR, Staines HM, Strecker T, Stubbe HC, Tsofa B, Zaki S, Fast P, Moorthy V, Kaiser L, Krishna S, Becker S, Kieny MP, Bejon P, Kremsner PG, Addo MM, Siegrist CA.
N Engl J Med. 2016 Apr 28;374(17):1647-60. doi: 10.1056/NEJMoa1502924.
Bornavirus Associated with Fatal Human Encephalitis.
Tappe D, Schmidt-Chanasit J, Beer M.
N Engl J Med. 2015 Nov 5;373(19):1880-1. doi: 10.1056/NEJMc1510342.
A Variegated Squirrel Bornavirus Associated with Fatal Human Encephalitis.
Hoffmann B, Tappe D, Höper D, Herden C, Boldt A, Mawrin C, Niederstraßer O, Müller T, Jenckel M, van der Grinten E, Lutter C, Abendroth B, Teifke JP, Cadar D, Schmidt-Chanasit J, Ulrich RG, Beer M.
N Engl J Med. 2015 Jul 9;373(2):154-62. doi: 10.1056/NEJMoa1415627.
A case of severe Ebola virus infection complicated by gram-negative septicemia.
Kreuels B, Wichmann D, Emmerich P, Schmidt-Chanasit J, de Heer G, Kluge S, Sow A, Renné T, Günther S, Lohse AW, Addo MM, Schmiedel S.
N Engl J Med. 2014 Dec 18;371(25):2394-401. doi: 10.1056/NEJMoa1411677.
Emergence of Zaire Ebola virus disease in Guinea.
Baize S, Pannetier D, Oestereich L, Rieger T, Koivogui L, Magassouba N, Soropogui B, Sow MS, Keïta S, De Clerck H, Tiffany A, Dominguez G, Loua M, Traoré A, Kolié M, Malano ER, Heleze E, Bocquin A, Mély S, Raoul H, Caro V, Cadar D, Gabriel M, Pahlmann M, Tappe D, Schmidt-Chanasit J, Impouma B, Diallo AK, Formenty P, Van Herp M, Günther S.
N Engl J Med. 2014 Oct 9;371(15):1418-25. doi: 10.1056/NEJMoa1404505.
Experimental risk assessment for chikungunya virus transmission based on vector competence, distribution and temperature suitability in Europe, 2018.
Heitmann A, Jansen S, Lühken R, Helms M, Pluskota B, Becker N, Kuhn C, Schmidt-Chanasit J, Tannich E.
Euro Surveill. 2018 Jul;23(29). doi: 10.2807/1560-7917.ES.2018.23.29.1800033.
Viral metagenomics, genetic and evolutionary characteristics of Crimean-Congo hemorrhagic fever orthonairovirus in humans, Kosovo.
Emmerich P, Jakupi X, von Possel R, Berisha L, Halili B, Günther S, Cadar D, Ahmeti S, Schmidt-Chanasit J.
Infect Genet Evol. 2018 Jul 11;65:6-11. doi: 10.1016/j.meegid.2018.07.010.
Yellow fever in the diagnostic laboratory.
Domingo C, Charrel RN, Schmidt-Chanasit J, Zeller H, Reusken C.
Emerg Microbes Infect. 2018 Jul 12;7(1):129. doi: 10.1038/s41426-018-0128-8.
Circulation of Dirofilaria immitis and Dirofilaria repens in the Danube Delta Biosphere Reserve, Romania.
Tomazatos A, Cadar D, Török E, Maranda I, Horváth C, Keresztes L, Spinu M, Jansen S, Jöst H, Schmidt-Chanasit J, Tannich E, Lühken R.
Parasit Vectors. 2018 Jul 4;11(1):392. doi: 10.1186/s13071-018-2980-8.
Semen inhibits Zika virus infection of cells and tissues from the anogenital region.
Müller JA, Harms M, Krüger F, Groß R, Joas S, Hayn M, Dietz AN, Lippold S, von Einem J, Schubert A, Michel M, Mayer B, Cortese M, Jang KS, Sandi-Monroy N, Deniz M, Ebner F, Vapalahti O, Otto M, Bartenschlager R, Herbeuval JP, Schmidt-Chanasit J, Roan NR, Münch J.
Nat Commun. 2018 Jun 7;9(1):2207. doi: 10.1038/s41467-018-04442-y.
Occupation-Associated Fatal Limbic Encephalitis Caused by Variegated Squirrel Bornavirus 1, Germany, 2013.
Tappe D, Schlottau K, Cadar D, Hoffmann B, Balke L, Bewig B, Hoffmann D, Eisermann P, Fickenscher H, Krumbholz A, Laufs H, Huhndorf M, Rosenthal M, Schulz-Schaeffer W, Ismer G, Hotop SK, Brönstrup M, Ott A, Schmidt-Chanasit J, Beer M.
Emerg Infect Dis. 2018 Jun;24(6):978-987. doi: 10.3201/eid2406.172027.
The molecular tweezer CLR01 inhibits Ebola and Zika virus infection.
Röcker AE, Müller JA, Dietzel E, Harms M, Krüger F, Heid C, Sowislok A, Riber CF, Kupke A, Lippold S, von Einem J, Beer J, Knöll B, Becker S, Schmidt-Chanasit J, Otto M, Vapalahti O, Zelikin AN, Bitan G, Schrader T, Münch J.
Antiviral Res. 2018 Apr;152:26-35. doi: 10.1016/j.antiviral.2018.02.003.
Culex torrentium mosquitoes from Germany are negative for Wolbachia.
Leggewie M, Krumkamp R, Badusche M, Heitmann A, Jansen S, Schmidt-Chanasit J, Tannich E, Becker SC.
Med Vet Entomol. 2018 Mar;32(1):115-120. doi: 10.1111/mve.12270.
Vector competence of field populations of the mosquito species Aedes japonicus japonicus and Culex pipiens from Switzerland for two West Nile virus strains.
Wagner S, Mathis A, Schönenberger AC, Becker S, Schmidt-Chanasit J, Silaghi C, Veronesi E.
Med Vet Entomol. 2018 Mar;32(1):121-124. doi: 10.1111/mve.12273.
Distribution of Usutu Virus in Germany and Its Effect on Breeding Bird Populations.
Lühken R, Jöst H, Cadar D, Thomas SM, Bosch S, Tannich E, Becker N, Ziegler U, Lachmann L, Schmidt-Chanasit J.
Emerg Infect Dis. 2017 Dec;23(12):1994-2001. doi: 10.3201/eid2312.171257
Specific detection of dengue and Zika virus antibodies using envelope proteins with mutations in the conserved fusion loop.
Rockstroh A, Moges B, Barzon L, Sinigaglia A, Palù G, Kumbukgolla W, Schmidt-Chanasit J, Sarno M, Brites C, Moreira-Soto A, Drexler JF, Ferreira OC, Ulbert S.
Emerg Microbes Infect. 2017 Nov 8;6(11):e99. doi: 10.1038/emi.2017.87
Detection and characterization of a novel rhabdovirus in Aedes cantans mosquitoes and evidence for a mosquito-associated new genus in the family Rhabdoviridae.
Shahhosseini N, Lühken R, Jöst H, Jansen S, Börstler J, Rieger T, Krüger A, Yadouleton A, de Mendonça Campos R, Cirne-Santos CC, Ferreira DF, Garms R, Becker N, Tannich E, Cadar D, Schmidt-Chanasit J.
Infect Genet Evol. 2017 Sep 21;55:260-268. doi: 10.1016/j.meegid.2017.09.026.
Compensating for cross-reactions using avidity and computation in a suspension multiplex immunoassay for serotyping of Zika versus other flavivirus infections.
Rönnberg B, Gustafsson Å, Vapalahti O, Emmerich P, Lundkvist Å, Schmidt-Chanasit J, Blomberg J.
Med Microbiol Immunol. 2017 Aug 29. doi: 10.1007/s00430-017-0517-y.
West Nile Virus lineage-2 in Culex specimens from Iran.
Shahhosseini N, Chinikar S, Moosa-Kazemi SH, Sedaghat MM, Kayedi MH, Lühken R, Schmidt-Chanasit J.
Trop Med Int Health. 2017 Jul 26. doi: 10.1111/tmi.12935.
Early Evidence for Zika Virus Circulation among Aedes aegypti Mosquitoes, Rio de Janeiro, Brazil.
Ayllón T, Campos RM, Brasil P, Morone FC, Câmara DCP, Meira GLS, Tannich E, Yamamoto KA, Carvalho MS, Pedro RS, Schmidt-Chanasit J, Cadar D, Ferreira DF, Honório NA.
Emerg Infect Dis. 2017 Aug 15;23(8). doi: 10.3201/eid2308.162007.
Blood donor screening for West Nile virus (WNV) revealed acute Usutu virus (USUV) infection, Germany, September 2016.
Cadar D, Maier P, Müller S, Kress J, Chudy M, Bialonski A, Schlaphof A, Jansen S, Jöst H, Tannich E, Runkel S, Hitzler WE, Hutschenreuter G, Wessiepe M, Schmidt-Chanasit J.
Euro Surveill. 2017;22(14):pii=30501. DOI: http://dx.doi.org/10.2807/1560-7917.
No serological evidence for Zika virus infection and low specificity for anti-Zika virus ELISA in malaria positive individuals among pregnant women from Madagascar in 2010.
Schwarz NG, Mertens E, Winter D, Maiga-Ascofaré O, Dekker D, Jansen S, Tappe D, Randriamampionona N, May J, Rakotozandrindrainy R, Schmidt-Chanasit J.
PLoS One. 2017 May 16;12(5):e0176708. doi: 10.1371/journal.pone.0176708.
Increased Proinflammatory Cytokine Levels in Prolonged Arthralgia in Ross River Virus Infection.
Tappe D, Pérez-Girón JV, Gómez-Medina S, Günther S, Muñoz-Fontela C, Schmidt-Chanasit J.
Emerg Infect Dis. 2017 Apr;23(4):702-704. doi: 10.3201/eid2304.160466.
Widespread activity of multiple lineages of Usutu virus, western Europe, 2016.
Cadar D, Lühken R, van der Jeugd H, Garigliany M, Ziegler U, Keller M, Lahoreau J, Lachmann L, Becker N, Kik M, Oude Munnink BB, Bosch S, Tannich E, Linden A, Schmidt V, Koopmans MP, Rijks J, Desmecht D, Groschup MH, Reusken C, Schmidt-Chanasit J.
Euro Surveill. 2017 Jan 26;22(4). pii: 30452. doi: 10.2807/1560-7917.ES.2017.22.4.30452.
Genetic Diversity and New Lineages of Dengue Virus Serotypes 3 and 4 in Returning Travelers, Germany, 2006-2015.
Shihada S, Emmerich P, Thomé-Bolduan C, Jansen S, Günther S, Frank C, Schmidt-Chanasit J, Cadar D.
Emerg Infect Dis. 2017 Feb;23(2):272-275. doi: 10.3201/eid2302.160751.
Recent Zika Virus Isolates Induce Premature Differentiation of Neural Progenitors in Human Brain Organoids.
Gabriel E, Ramani A, Karow U, Gottardo M, Natarajan K, Gooi LM, Goranci-Buzhala G, Krut O, Peters F, Nikolic M, Kuivanen S, Korhonen E, Smura T, Vapalahti O, Papantonis A, Schmidt-Chanasit J, Riparbelli M, Callaini G, Krönke M, Utermöhlen O, Gopalakrishnan J.
Cell Stem Cell. 2017 Jan 23. pii: S1934-5909(16)30461-1. doi: 10.1016/j.stem.2016.12.005.
Experimental transmission of Zika virus by mosquitoes from central Europe.
Heitmann A, Jansen S, Lühken R, Leggewie M, Badusche M, Pluskota B, Becker N, Vapalahti O, Schmidt-Chanasit J, Tannich E.
Euro Surveill. 2017 Jan 12;22(2). pii: 30437. doi: 10.2807/1560-7917.ES.2017.22.2.30437.
Development of a high-throughput colorimetric Zika virus infection assay.
Müller JA, Harms M, Schubert A, Mayer B, Jansen S, Herbeuval JP, Michel D, Mertens T, Vapalahti O, Schmidt-Chanasit J, Münch J.
Med Microbiol Immunol. 2017 Feb 7. doi: 10.1007/s00430-017-0493-2.
Yellow fever in a traveller returning from Suriname to the Netherlands, March 2017.
Wouthuyzen-Bakker M, Knoester M, van den Berg AP, GeurtsvanKessel CH, Koopmans MP, Van Leer-Buter C, Oude Velthuis B, Pas SD, Ruijs WL, Schmidt-Chanasit J, Vreden SG, van der Werf TS, Reusken CB, Bierman WF.
Euro Surveill. 2017;22(11):pii=30488. DOI: http://dx.doi.org/10.2807/1560-7917.ES.2017.22.11.30488
Variegated Squirrel Bornavirus 1 in Squirrels, Germany and the Netherlands.
Schlottau K, Jenckel M, van den Brand J, Fast C, Herden C, Höper D, Homeier-Bachmann T, Thielebein J, Mensing N, Diender B, Hoffmann D, Ulrich RG, Mettenleiter TC, Koopmans M, Tappe D, Schmidt-Chanasit J, Reusken CB, Beer M, Hoffmann B.
Emerg Infect Dis. 2017 Mar;23(3):477-481. doi: 10.3201/eid2303.161061.
Serodiagnosis of Zika virus (ZIKV) infections by a novel NS1-based ELISA devoid of cross-reactivity with dengue virus antibodies: a multicohort study of assay performance, 2015 to 2016.
Steinhagen K, Probst C, Radzimski C, Schmidt-Chanasit J, Emmerich P, van Esbroeck M, Schinkel J, Grobusch MP, Goorhuis A, Warnecke JM, Lattwein E, Komorowski L, Deerberg A, Saschenbrecker S, Stöcker W, Schlumberger W.
Euro Surveill. 2016 Dec 15;21(50). pii: 30426. doi: 10.2807/1560-7917.ES.2016.21.50.30426.
High specificity of a novel Zika virus ELISA in European patients after exposure to different flaviviruses.
Huzly D, Hanselmann I, Schmidt-Chanasit J, Panning M.
Euro Surveill. 2016 Apr 21;21(16). doi: 10.2807/1560-7917.ES.2016.21.16.30203.
Assay optimization for molecular detection of Zika virus.
Corman VM, Rasche A, Baronti C, Aldabbagh S, Cadar D, Reusken CB, Pas SD, Goorhuis A, Schinkel J, Molenkamp R, Kümmerer BM, Bleicker T, Brünink S, Eschbach-Bludau M, Eis-Hübinger AM, Koopmans MP, Schmidt-Chanasit J, Grobusch MP, de Lamballerie X, Drosten C, Drexler JF.
Bull World Health Organ. 2016 Dec 1;94(12):880-892.
Inactivation and Environmental Stability of Zika Virus.
Müller JA, Harms M, Schubert A, Jansen S, Michel D, Mertens T, Schmidt-Chanasit J, Münch J.
Emerg Infect Dis. 2016 Sep 15;22(9). doi: 10.3201/eid2209.160664
Sexual transmission of Zika virus in Germany, April 2016.
Frank C, Cadar D, Schlaphof A, Neddersen N, Günther S, Schmidt-Chanasit J, Tappe D.
Euro Surveill. 2016 Jun 9;21(23). doi: 10.2807/1560-7917.ES.2016.21.23.30252.
Cytokine kinetics of Zika virus-infected patients from acute to reconvalescent phase.
Tappe D, Pérez-Girón JV, Zammarchi L, Rissland J, Ferreira DF, Jaenisch T, Gómez-Medina S, Günther S, Bartoloni A, Muñoz-Fontela C, Schmidt-Chanasit J.
Med Microbiol Immunol. 2016 10.1007/s00430-015-0445-7
Confirmed Zika virus infection in a Belgian traveler returning from Guatemala, and the diagnostic challenges of imported cases into Europe.
De Smet B, Van den Bossche D, van de Werve C, Mairesse J, Schmidt-Chanasit J, Michiels J, Ariën KK, Van Esbroeck M, Cnops L.
J Clin Virol. 2016 Jul;80:8-11. doi: 10.1016/j.jcv.2016.04.009
Prolonged detection of Zika virus RNA in urine samples during the ongoing Zika virus epidemic in Brazil.
de M Campos R, Cirne-Santos C, Meira GL, Santos LL, de Meneses MD, Friedrich J, Jansen S, Ribeiro MS, da Cruz IC, Schmidt-Chanasit J, Ferreira DF.
J Clin Virol. 2016 Feb 16;77:69-70. doi: 10.1016/j.jcv.2016.02.009.
Zika without symptoms in returning travellers: What are the implications?
Ginier M, Neumayr A, Günther S, Schmidt-Chanasit J, Blum J.
Travel Med Infect Dis. 2016 Jan-Feb;14(1):16-20. doi: 10.1016/j.tmaid.2016.01.012.
False positive dengue NS1 antigen test in a traveller with an acute Zika virus infection imported into Switzerland.
Gyurech D, Schilling J, Schmidt-Chanasit J, Cassinotti P, Kaeppeli F, Dobec M.
Swiss Med Wkly. 2016 Feb 9;146:w14296. doi: 10.4414/smw.2016.14296.
Reconstruction of the Evolutionary History and Dispersal of Usutu Virus, a Neglected Emerging Arbovirus in Europe and Africa.
Engel D, Jöst H, Wink M, Börstler J, Bosch S, Garigliany MM, Jöst A, Czajka C, Lühken R, Ziegler U, Groschup MH, Pfeffer M, Becker N, Cadar D, Schmidt-Chanasit J.
MBio. 2016 Feb 2;7(1). pii: e01938-15. doi: 10.1128/mBio.01938-15.
Pilot longitudinal mosquito surveillance study in the Danube Delta Biosphere Reserve and the first reports of Anopheles algeriensis Theobald, 1903 and Aedes hungaricus Mihályi, 1955 for Romania.
Török E, Tomazatos A, Cadar D, Horváth C, Keresztes L, Jansen S, Becker N, Kaiser A, Popescu O, Schmidt-Chanasit J, Jöst H, Lühken R.
Parasit Vectors. 2016 Apr 11;9(1):196. doi: 10.1186/s13071-016-1484-7.
Sustained Elevated Cytokine Levels during Recovery Phase of Mayaro Virus Infection.
Tappe D, Pérez-Girón JV, Just-Nübling G, Schuster G, Gómez-Medina S, Günther S, Muñoz-Fontela C, Schmidt-Chanasit J.
Emerg Infect Dis. 2016 Apr;22(4):750-2. doi: 10.3201/eid2204.151502.
Host-feeding patterns of mosquito species in Germany.
Börstler J, Jöst H, Garms R, Krüger A, Tannich E, Becker N, Schmidt-Chanasit J, Lühken R.
Parasit Vectors. 2016 Jun 3;9(1):318. doi: 10.1186/s13071-016-1597-z.
Surveillance of maternal antibodies against West Nile virus in chicken eggs in South-West Germany.
Börstler J, Engel D, Petersen M, Poggensee C, Jansen S, Schmidt-Chanasit J, Lühken R.
Trop Med Int Health. 2016 May;21(5):687-90. doi: 10.1111/tmi.12676.
Distribution of individual members of the mosquito Anopheles maculipennis complex in Germany identified by newly developed real-time PCR assays.
Lühken R, Czajka C, Steinke S, Jöst H, Schmidt-Chanasit J, Pfitzner W, Becker N, Kiel E, Krüger A, Tannich E.
Med Vet Entomol. 2016 Jun;30(2):144-54. doi: 10.1111/mve.12161.
New Hosts of The Lassa Virus.
Olayemi A, Cadar D, Magassouba N, Obadare A, Kourouma F, Oyeyiola A, Fasogbon S, Igbokwe J, Rieger T, Bockholt S, Jérôme H, Schmidt-Chanasit J, Garigliany M, Lorenzen S, Igbahenah F, Fichet JN, Ortsega D, Omilabu S, Günther S, Fichet-Calvet E.
Sci Rep. 2016 May 3;6:25280. doi: 10.1038/srep25280.
Indoor development of Aedes aegypti in Germany, 2016.
Kampen H, Jansen S, Schmidt-Chanasit J, Walther D.
Euro Surveill. 2016 Nov 24;21(47). pii: 30407. doi: 10.2807/1560-7917.ES.2016.21.47.30407.
Evidence for an independent third Usutu virus introduction into Germany.
Ziegler U, Fast C, Eiden M, Bock S, Schulze C, Hoeper D, Ochs A, Schlieben P, Keller M, Zielke DE, Luehken R, Cadar D, Walther D, Schmidt-Chanasit J, Groschup MH.
Vet Microbiol. 2016 Aug 30;192:60-6. doi: 10.1016/j.vetmic.2016.06.007.
Co-circulation of a novel phlebovirus and Massilia virus in sandflies, Portugal.
Amaro F, Zé-Zé L, Alves MJ, Börstler J, Clos J, Lorenzen S, Becker SC, Schmidt-Chanasit J, Cadar D.
Virol J. 2015 Oct 24;12:174. doi: 10.1186/s12985-015-0407-0.
Putative Lineage of Novel African Usutu Virus, Central Europe.
Cadar D, Bosch S, Jöst H, Börstler J, Garigliany MM, Becker N, Schmidt-Chanasit J.
Emerg Infect Dis. 2015 Sep [date cited]. http://dx.doi.org/10.3201/eid2109.142026
Zika virus infection in a traveller returning to Europe from Brazil, March 2015.
Zammarchi L, Tappe D, Fortuna C, Remoli ME, Gunther S, Venturi G, Bartoloni A, Schmidt-Chanasit J.
Euro Surveill. 2015 Jun 11;20(23). pii: 21153.
Acute Zika virus infection after travel to Malaysian Borneo, September 2014.
Tappe D, Nachtigall S, Kapaun A, Schnitzler P, Günther S, Schmidt-Chanasit J.
Emerg Infect Dis. 2015 May;21(5):911-3. doi: 10.3201/eid2105.141960.
Zika virus infections imported to Italy: clinical, immunological and virological findings, and public health implications.
Zammarchi L, Stella G, Mantella A, Bartolozzi D, Tappe D, Günther S, Oestereich L, Cadar D, Muñoz-Fontela C, Bartoloni A, Schmidt-Chanasit J.
J Clin Virol. 2015 Feb;63:32-5. doi: 10.1016/j.jcv.2014.12.005.
Surveillance of Batai virus in bovines from Germany.
Hofmann M, Wiethölter A, Blaha I, Jöst H, Heinemann P, Lehmann M, Miller T, Cadar D, Yanase T, Kley N, Eiden M, Groschup M, Schmidt-Chanasit J.
Clin Vaccine Immunol. 2015 Jun;22(6):672-3. doi: 10.1128/CVI.00082-15.
West Nile virus lineage 2 infection in a blood donor from Vienna, Austria, August 2014.
Jungbauer C, Hourfar MK, Stiasny K, Aberle SW, Cadar D, Schmidt-Chanasit J, Mayr WR.
J Clin Virol. 2015 Mar;64:16-9. doi: 10.1016/j.jcv.2015.01.003.
Characterization of a novel circo-like virus in Aedes vexans mosquitoes from Germany: evidence for a new genus within the family Circoviridae.
Garigliany MM, Börstler J, Jöst H, Badusche M, Desmecht D, Schmidt-Chanasit J, Cadar D.
J Gen Virol. 2015 Apr;96(Pt 4):915-20. doi: 10.1099/vir.0.000036.
Health care response to CCHF in US soldier and nosocomial transmission to health care providers, Germany, 2009.
Conger NG, Paolino KM, Osborn EC, Rusnak JM, Günther S, Pool J, Rollin PE, Allan PF, Schmidt-Chanasit J, Rieger T, Kortepeter MG.
Emerg Infect Dis. 2015 Jan;21(1):23-31. doi: 10.3201/eid2101.141413.
Usutu virus in bats, Germany, 2013.
Cadar D, Becker N, Campos Rde M, Börstler J, Jöst H, Schmidt-Chanasit J.
Emerg Infect Dis. 2014 Oct;20(10):1771-3. doi: 10.3201/eid2010.140909.
O'nyong-nyong virus infection imported to Europe from Kenya by a traveler.
Tappe D, Kapaun A, Emmerich P, Campos Rde M, Cadar D, Günther S, Schmidt-Chanasit J.
Emerg Infect Dis. 2014 Oct;20(10):1766-7. doi: 10.3201/eid2010.140823.
Zika virus infection after travel to Tahiti, December 2013.
Wæhre T, Maagard A, Tappe D, Cadar D, Schmidt-Chanasit J.
Emerg Infect Dis. 2014 Aug;20(8):1412-4. doi: 10.3201/eid2008.140302.
Dengue virus transmission by blood stem cell donor after travel to Sri Lanka; Germany, 2013.
Punzel M, Korukluoğlu G, Caglayik DY, Menemenlioglu D, Bozdag SC, Tekgündüz E, Altuntaş F, Campos Rde M, Burde B, Günther S, Tappe D, Cadar D, Schmidt-Chanasit J.
Emerg Infect Dis. 2014 Aug;20(8):1366-9. doi: 10.3201/eid2008.140508.
Genome analysis of Mayaro virus imported to Germany from French Guiana.
Friedrich-Jänicke B, Emmerich P, Tappe D, Günther S, Cadar D, Schmidt-Chanasit J.
Emerg Infect Dis. 2014 Jul;20(7):1255-7. doi: 10.3201/eid2007.140043.
First case of laboratory-confirmed Zika virus infection imported into Europe, November 2013.
Tappe D, Rissland J, Gabriel M, Emmerich P, Gunther S, Held G, Smola S, Schmidt-Chanasit J.
Euro Surveill. 2014 Jan 30;19(4). pii: 20685.
Autochthonous dengue virus infection in Japan imported into Germany, September 2013.
Schmidt-Chanasit J, Emmerich P, Tappe D, Gunther S, Schmidt S, Wolff D, Hentschel K, Sagebiel D, Schoneberg I, Stark K, Frank C.
Euro Surveill. 2014 Jan 23;19(3). pii: 20681.
Detection of Usutu virus in a bullfinch (Pyrrhula pyrrhula) and a great spotted woodpecker (Dendrocopos major) in north-west Europe.
Garigliany MM, Marlier D, Tenner-Racz K, Eiden M, Cassart D, Gandar F, Beer M, Schmidt-Chanasit J, Desmecht D.
Vet J. 2014 Jan;199(1):191-3. doi: 10.1016/j.tvjl.2013.10.017.
Dengue virus & co: are mosquito-borne viral diseases on the rise?
Cramer JP, Schmidt-Chanasit J.
Dtsch Med Wochenschr. 2014 Feb;139(6):247-50. doi: 10.1055/s-0033-1359968.
Cyclovirus CyCV-VN species distribution is not limited to Vietnam and extends to Africa.
Garigliany MM, Hagen RM, Frickmann H, May J, Schwarz NG, Perse A, Jöst H, Börstler J, Shahhosseini N, Desmecht D, Mbunkah HA, Daniel AM, Kingsley MT, de Mendonca Campos R, de Paula VS, Randriamampionona N, Poppert S, Tannich E, Rakotozandrindrainy R, Cadar D, Schmidt-Chanasit J.
Sci Rep. 2014 Dec 18;4:7552. doi: 10.1038/srep07552.
Lack of evidence for the presence of Schmallenberg virus in mosquitoes in Germany, 2011.
Wernike K, Jöst H, Becker N, Schmidt-Chanasit J, Beer M
Parasit Vectors. 2014 Aug 29;7:402. doi: 10.1186/1756-3305-7-402.
Increase in West Nile virus infections imported to Germany in 2012.
Gabriel M, Emmerich P, Frank C, Fiedler M, Rashidi-Alavijeh J, Jochum C, Günther S, Auerhammer K, Rupprecht HJ, Blank RT, Sacher N, Pertzborn L, Stark K, Schrauzer T, Schmidt-Chanasit J.
J Clin Virol. 2013 Nov;58(3):587-9. doi: 10.1016/j.jcv.2013.08.027.
Emergence of Dengue virus 4 genotypes II b and I in the city of Rio de Janeiro.
Campos Rde M, Veiga CS, Meneses MD, de Souza LM, Fernandes CA, Malirat V, Albuquerque JP, Tanuri A, Schmidt-Chanasit J, Ferreira DF.
J Clin Virol. 2013 Jan;56(1):86-8. doi: 10.1016/j.jcv.2012.10.006.
Epizootic emergence of Usutu virus in wild and captive birds in Germany.
Becker N, Jöst H, Ziegler U, Eiden M, Höper D, Emmerich P, Fichet-Calvet E, Ehichioya DU, Czajka C, Gabriel M, Hoffmann B, Beer M, Tenner-Racz K, Racz P, Günther S, Wink M, Bosch S, Konrad A, Pfeffer M, Groschup MH, Schmidt-Chanasit J.
PLoS One. 2012;7(2):e32604. doi: 10.1371/journal.pone.0032604.
West Nile virus meningoencephalitis imported into Germany.
Schultze-Amberger J, Emmerich P, Günther S, Schmidt-Chanasit J.
Emerg Infect Dis. 2012 Oct;18(10):1698-700. doi: 10.3201/eid1810.120204.
Two laboratory-confirmed cases of Japanese encephalitis imported to Germany by travelers returning from Southeast Asia.
Tappe D, Nemecek A, Zipp F, Emmerich P, Gabriel M, Günther S, Dobler G, Schmidt-Chanasit J, Stich A.
J Clin Virol. 2012 Jul;54(3):282-5. doi: 10.1016/j.jcv.2012.03.004.
Fatal dengue hemorrhagic fever imported into Germany.
Schmidt-Chanasit J, Tenner-Racz K, Poppert D, Emmerich P, Frank C, Dinges C, Penning R, Nerlich A, Racz P, Günther S.
Infection. 2012 Aug;40(4):441-3. doi: 10.1007/s15010-011-0208-3.
Detection of Usutu virus infection in a healthy blood donor from south-west Germany, 2012.
Allering L, Jöst H, Emmerich P, Günther S, Lattwein E, Schmidt M, Seifried E, Sambri V, Hourfar K, Schmidt-Chanasit J.
Euro Surveill. 2012 Dec 13;17(50). pii: 20341.
Seroprevalence of antibodies against Chikungunya, Dengue, and Rift Valley fever viruses after febrile illness outbreak, Madagascar.
Schwarz NG, Girmann M, Randriamampionona N, Bialonski A, Maus D, Krefis AC, Njarasoa C, Rajanalison JF, Ramandrisoa HD, Randriarison ML, May J, Schmidt-Chanasit J, Rakotozandrindrainy R.
Emerg Infect Dis. 2012 Nov;18(11):1780-6. doi: 10.3201/eid1811.111036.
Isolation of usutu virus in Germany.
Jöst H, Bialonski A, Maus D, Sambri V, Eiden M, Groschup MH, Günther S, Becker N, Schmidt-Chanasit J.
Am J Trop Med Hyg. 2011 Sep;85(3):551-3. doi: 10.4269/ajtmh.2011.11-0248.
Medical importance of Sindbis virus in south-west Germany.
Jöst H, Bürck-Kammerer S, Hütter G, Lattwein E, Lederer S, Litzba N, Bock-Hensley O, Emmerich P, Günther S, Becker N, Niedrig M, Schmidt-Chanasit J.
J Clin Virol. 2011 Nov;52(3):278-9. doi: 10.1016/j.jcv.2011.08.002.
Isolation and phylogenetic analysis of Batai virus, Germany.
Jöst H, Bialonski A, Schmetz C, Günther S, Becker N, Schmidt-Chanasit J.
Am J Trop Med Hyg. 2011 Feb;84(2):241-3. doi: 10.4269/ajtmh.2011.10-0483.
Isolation and phylogenetic analysis of Sindbis viruses from mosquitoes in Germany.
Jöst H, Bialonski A, Storch V, Günther S, Becker N, Schmidt-Chanasit J.
J Clin Microbiol. 2010 May;48(5):1900-3. doi: 10.1128/JCM.00037-10.
Dr. med. Sami Shihada (2018) : High genetic diversity and emergence of new lineages of dengue virus type 3 and 4 detected in german travellers, 2006-2015. magna cum laude, University Medical Center Hamburg-Eppendorf
Dr. rer. nat. Nariman Shahhosseini (2017) : Mosquito surveillance in Iran and Germany reveals new insights into arbovirus ecology and evolution. cum laude, University of Hamburg
Dr. med. Dimitri Engel (2017) : Reconstruction of the evolutionary history and dispersal of Usutu virus, a neglected emerging arbovirus in Europe and Africa. magna cum laude, University Medical Center Hamburg-Eppendorf
Dr. rer. nat. Jessica Börstler (2016) : Arboviruses in Germany: geographical distribution and the interaction between mosquitoes and vertebrates. magna cum laude, University of Hamburg
Dr. med. Ludger Allering (2016) : Detection of Usutu virus infection in a healthy blood donor from south-west Germany, 2012. magna cum laude, University Medical Center Hamburg-Eppendorf
Dr. rer. nat. Hanna Jöst (2013) : Surveillance of arboviruses in mosquitoes of Germany. magna cum laude, Heidelberg University, Ruperto Carola
Dr. rer. nat. Patrick Heinemann (2013) : The N terminus of Andes virus L protein suppresses mRNA and protein expression in mammalian cells. magna cum laude, University of Bremen
Dr. med. Karoline Bleymehl (2011) : Phenotypic and genetic characterization of varicella-zoster virus mutants resistant to acyclovir, brivudine and/or foscarnet. summa cum laude, Goethe University Frankfurt
Dr. med. Stefanie Mikolajczak (2010) : Nephropathia epidemica outbreak in Lower Franconia and the Eifel: molecular biological studies on the causative hantavirus species in summer/autumn 2007. magna cum laude, Goethe University Frankfurt