Our publication highlights
Application of CRISPR/Cas9-Based Reverse Genetics in Leishmania braziliensis: Conserved Roles for HSP100 and HSP23
- Adaui V, Kröber-Boncardo C, Brinker C, Zirpel H, Sellau J, Arévalo J, Dujardin JC, Clos J
- Genes (Basel). 2020 Sep 30;11(10):1159. doi: 10.3390/genes11101159
Leishmania donovani 90 kD Heat Shock Protein - Impact of Phosphosites on Parasite Fitness, Infectivity and Casein Kinase Affinity
- Hombach-Barrigah A, Bartsch K, Smirlis D, Rosenqvist H, MacDonald A, Dingli F, Loew D, Späth GF, Rachidi N, Wiese M, Clos J
- Sci Rep. 2019 Mar 25;9(1):5074. doi: 10.1038/s41598-019-41640-0
Ribosome Profiling Reveals HSP90 Inhibitor Effects on Stage-Specific Protein Synthesis in Leishmania donovani
- Bifeld E, Lorenzen S, Bartsch K, Vasquez JJ, Siegel TN, Clos J
- mSystems. 2018 Nov 20;3(6):e00214-18. doi: 10.1128/mSystems.00214-18
Casein kinase 1.2 over expression restores stress resistance to Leishmania donovani HSP23 null mutants
- Kröber-Boncardo C, Lorenzen S, Brinker C, Clos J
- Sci Rep. 2020 Sep 29;10(1):15969. doi: 10.1038/s41598-020-72724-x
Life Cycle Stage-Specific Accessibility of Leishmania donovani Chromatin at Transcription Start Regions
- Grünebast J, Lorenzen S, Zummack J, Clos J
- mSystems. 2021 Aug 31;6(4):e0062821. doi: 10.1128/mSystems.00628-21
Our publications
2022
Promastigote-to-Amastigote Conversion in Leishmania spp.-A Molecular View
- Clos J, Grünebast J, Holm M
- Pathogens. 2022 Sep 15;11(9):1052. doi: 10.3390/pathogens11091052
Design, Synthesis and Antiparasitic Evaluation of Click Phospholipids
- George E Magoulas, Pantelis Afroudakis, Kalliopi Georgikopoulou, Marina Roussaki, Chiara Borsari, Theano Fotopoulou, Nuno Santarem, Emile Barrias, Paloma Tejera Nevado, Julia Hachenberg, Eugenia Bifeld, Bernhard Ellinger, Maria Kuzikov, Irini Fragiadaki, Effie Scoulica, Joachim Clos, Sheraz Gul, Maria Paola Costi, Wanderley de Souza, Kyriakos C Prousis, Anabela Cordeiro da Silva, Theodora Calogeropoulou
- Molecules. 2021 Jul 10;26(14):4204. doi: 10.3390/molecules26144204
2021
Life Cycle Stage-Specific Accessibility of Leishmania donovani Chromatin at Transcription Start Regions
- Grünebast J, Lorenzen S, Zummack J, Clos J
- mSystems. 2021 Aug 31;6(4):e0062821. doi: 10.1128/mSystems.00628-21
Repurposing Carvedilol as a Novel Inhibitor of the Trypanosoma cruzi Autophagy Flux That Affects Parasite Replication and Survival
- Rivero CV, Martínez SJ, Novick P, Cueto JA, Salassa BN, Vanrell MC, Li X, Labriola CA, Polo LM, Engman DM, Clos J, Romano PS
- Front Cell Infect Microbiol. 2021 Aug 12;11:657257. doi: 10.3389/fcimb.2021.657257
High Content Analysis of Macrophage-Targeting EhPIb-Compounds against Cutaneous and Visceral Leishmania Species
- Fehling H, Niss H, Bea A, Kottmayr N, Brinker C, Hoenow S, Sellau J, Gilberger TW, Ting F, Landschulze D, Meier C, Clos J, Lotter H
- Microorganisms. 2021 Feb 18;9(2):422. doi: 10.3390/microorganisms9020422
2020
Leishmania: Responding to environmental signals and challenges without regulated transcription
- Grünebast J, Clos J
- Comput Struct Biotechnol J. 2020 Dec 7;18:4016-4023. doi: 10.1016/j.csbj.2020.11.058
Application of CRISPR/Cas9-Based Reverse Genetics in Leishmania braziliensis: Conserved Roles for HSP100 and HSP23
- Adaui V, Kröber-Boncardo C, Brinker C, Zirpel H, Sellau J, Arévalo J, Dujardin JC, Clos J
- Genes (Basel). 2020 Sep 30;11(10):1159. doi: 10.3390/genes11101159
The Leishmania donovani SENP Protease Is Required for SUMO Processing but Not for Viability
- Bea A, Kröber-Boncardo C, Sandhu M, Brinker C, Clos J
- Genes (Basel). 2020 Oct 14;11(10):1198. doi: 10.3390/genes11101198
Antileishmanial Effects of Synthetic EhPIb Analogs Derived from the Entamoeba histolytica Lipopeptidephosphoglycan
- Fehling H, Choy SL, Ting F, Landschulze D, Bernin H, Lender SC, Mühlenpfordt M, Bifeld E, Eick J, Marggraff C, Kottmayr N, Groneberg M, Hoenow S, Sellau J, Clos J, Meier C, Lotter H
- Antimicrob Agents Chemother. 2020 Jun 23;64(7):e00161-20. doi: 10.1128/AAC.00161-20
Casein kinase 1.2 over expression restores stress resistance to Leishmania donovani HSP23 null mutants
- Kröber-Boncardo C, Lorenzen S, Brinker C, Clos J
- Sci Rep. 2020 Sep 29;10(1):15969. doi: 10.1038/s41598-020-72724-x
Heat Shock Proteins in Leishmania Parasites
- Kröber-Boncardo C., Grünebast J., Clos J.
- In: . Heat Shock Proteins. Springer, Dordrecht. https://doi.org/10.1007/7515_2020_27
2019
Discovery of a benzothiophene-flavonol halting miltefosine and antimonial drug resistance in Leishmania parasites through the application of medicinal chemistry, screening and genomics
- Borsari C, Jiménez-Antón MD, Eick J, Bifeld E, Torrado JJ, Olías-Molero AI, Corral MJ, Santarem N, Baptista C, Severi L, Gul S, Wolf M, Kuzikov M, Ellinger B, Reinshagen J, Witt G, Linciano P, Tait A, Costantino L, Luciani R, Tejera Nevado P, Zander-Dinse D, Franco CH, Ferrari S, Moraes CB, Cordeiro-da-Silva A, Ponterini G, Clos J, Alunda JM, Costi MP
- Eur J Med Chem. 2019 Dec 1;183:111676. doi: 10.1016/j.ejmech.2019.111676
Gene Replacement by Homologous Recombination
- Zirpel H, Clos J
- Methods Mol Biol. 2019;1971:169-188. doi: 10.1007/978-1-4939-9210-2_8
Cosmid Library Construction and Functional Cloning
- Clos J, Zander-Dinse D
- Methods Mol Biol. 2019;1971:123-140. doi: 10.1007/978-1-4939-9210-2_6
Generation of Bone Marrow-Derived Macrophages for In Vitro Infection Experiments
- Bifeld E
- Methods Mol Biol. 2019;1971:237-247. doi: 10.1007/978-1-4939-9210-2_12
Quantification of Intracellular Leishmania spp. Using Real-Time Quantitative PCR (qPCR)
- Bifeld, E
- Methods Mol Biol. 2019;1971:249-263. doi: 10.1007/978-1-4939-9210-2_13
Leishmania donovani 90 kD Heat Shock Protein - Impact of Phosphosites on Parasite Fitness, Infectivity and Casein Kinase Affinity
- Hombach-Barrigah A, Bartsch K, Smirlis D, Rosenqvist H, MacDonald A, Dingli F, Loew D, Späth GF, Rachidi N, Wiese M, Clos J
- Sci Rep. 2019 Mar 25;9(1):5074. doi: 10.1038/s41598-019-41640-0
Pharmacological Validation of N-Myristoyltransferase as a Drug Target in Leishmania donovani
- Corpas-Lopez V, Moniz S, Thomas M, Wall RJ, Torrie LS, Zander-Dinse D, Tinti M, Brand S, Stojanovski L, Manthri S, Hallyburton I, Zuccotto F, Wyatt PG, De Rycker M, Horn D, Ferguson MAJ, Clos J, Read KD, Fairlamb AH, Gilbert IH, Wyllie S
- ACS Infect Dis. 2019 Jan 11;5(1):111-122. doi: 10.1021/acsinfecdis.8b00226
Accelerating Drug Discovery Efforts for Trypanosomatidic Infections Using an Integrated Transnational Academic Drug Discovery Platform.
- Moraes CB, Witt G, Kuzikov M, Ellinger B, Calogeropoulou T, Prousis KC, Mangani S, Di Pisa F, Landi G, Iacono LD, Pozzi C, Freitas-Junior LH, Dos Santos Pascoalino B, Bertolacini CP, Behrens B, Keminer O, Leu J, Wolf M, Reinshagen J, Cordeiro-da-Silva A, Santarem N, Venturelli A, Wrigley S, Karunakaran D, Kebede B, Pöhner I, Müller W, Panecka-Hofman J, Wade RC, Fenske M, Clos J, Alunda JM, Corral MJ, Uliassi E, Bolognesi ML, Linciano P, Quotadamo A, Ferrari S, Santucci M, Borsari C, Costi MP, Gul S
- SLAS Discov. 2019 Mar;24(3):346-361. doi: 10.1177/2472555218823171
Leishmania - Methods and Protocols
- Clos, J. ed.
- Methods in Molecular Biology (2019) 1971, 1-368
2018
Ribosome Profiling Reveals HSP90 Inhibitor Effects on Stage-Specific Protein Synthesis in Leishmania donovani
- Bifeld E, Lorenzen S, Bartsch K, Vasquez JJ, Siegel TN, Clos J
- mSystems. 2018 Nov 20;3(6):e00214-18. doi: 10.1128/mSystems.00214-18
Molecular Preadaptation to Antimony Resistance in Leishmania donovani on the Indian Subcontinent
- Dumetz F, Cuypers B, Imamura H, Zander D, D'Haenens E, Maes I, Domagalska MA, Clos J, Dujardin JC, De Muylder G
- mSphere. 2018 Apr 18;3(2):e00548-17. doi: 10.1128/mSphere.00548-17
2017
MAPK1 of Leishmania donovani interacts and phosphorylates HSP70 and HSP90 subunits of foldosome complex
- Kaur P, Garg M, Hombach-Barrigah A, Clos J, Goyal N
- Sci Rep. 2017 Aug 31;7(1):10202. doi: 10.1038/s41598-017-09725-w
Synthetic analogs of an Entamoeba histolytica glycolipid designed to combat intracellular Leishmania infection
- Choy SL, Bernin H, Aiba T, Bifeld E, Lender SC, Mühlenpfordt M, Noll J, Eick J, Marggraff C, Niss H, Roldán NG, Tanaka S, Kitamura M, Fukase K, Clos J, Tannich E, Fujimoto Y, Lotter H
- Sci Rep. 2017 Aug 25;7(1):9472. doi: 10.1038/s41598-017-09894-8
Characterization of the protein tyrosine phosphatase LmPRL-1 secreted by Leishmania major via the exosome pathway
- Leitherer S, Clos J, Liebler-Tenorio EM, Schleicher U, Bogdan C and Soulat D
- Infect Immun. 2017 Jul 19;85(8):e00084-17. doi: 10.1128/IAI.00084-17
Hsp90 Inhibitors radicicol and geldanamycin have opposing effects onLeishmaniaAha1-dependent proliferation
- Bartsch K, Hombach-Barrigah A, Clos J
- Cell Stress Chaperones. 2017 Sep;22(5):729-742. doi: 10.1007/s12192-017-0800-2
Leishmania donovani chaperonin 10 regulates parasite internalization and intracellular survival in human macrophages
- Colineau L, Clos J, Moon KM, Foster LJ, Reiner NE
- Med Microbiol Immunol. 2017 Jun;206(3):235-257. doi: 10.1007/s00430-017-0500-7
Methoxylated 2'-hydroxychalcones as antiparasitic hit compounds
- Borsari C, Santarem N, Torrado J, Olías AI, Corral MJ, Baptista C, Gul S, Wolf M, Kuzikov M, Ellinger B, Reinshagen J, Linciano P, Tait A, Costantino L, Freitas-Junior LH, Moraes CB, Pascoalino B, Alcântara LMaria, Franco CH, Bertolacini CD, Fontana V, Tejera Nevado P, Clos J, Alunda JM, Cordeiro-da-Silva A, Ferrari S, Costi MP
- Eur J Med Chem. 2017 Jan 27;126:1129-1135. doi: 10.1016/j.ejmech.2016.12.017
Leishmania Heat Shock Proteins as Effectors of Immune Evasion and Virulence
- Bartsch K, Eick J, Zirpel H and Clos J
- Current Immunology Reviews, 2017, 13 - accepted
2016
Profiling of flavonol derivatives for the development of anti-trypanosomatidic drugs
- Borsari C, Luciani R, Pozzi C, Pohner I, Henrich S, Trande M, Cordeiro-da-Silva A, Santarem N, Baptista C, Tait A, Di Pisa F, Dello Iacono L, Landi G, Gul S, Wolf M, Kuzikov M, Ellinger B, Reinshagen J, Witt G, Gribbon P, Kohler M, Keminer O, Behrens B, Costantino L, Tejera Nevado P, Bifeld E, Eick J, Clos J, Torrado J, Jimenez-Anton MD, Corral MJ, Alunda JM, Pellati F, Wade RC, Ferrari S, Mangani S, Costi MP
- J Med Chem. 2016 Aug 25;59(16):7598-616. doi: 10.1021/acs.jmedchem.6b0069
Phenotypic Characterization of a Leishmania donovani Cyclophyilin 40 Null Mutant
- Wai-Lok Y, Lambertz U, Colineau L, Pescher P, MacDonald A, Zander D, Retzlaff S, Eick J, Reiner NE, Clos J, Späth GF
- J Eukaryot Microbiol. 2016 Nov;63(6):823-833. doi: 10.1111/jeu.12329
Joining forces: first application of a rapamycin-induced dimerizable Cre system for conditional null mutant analysis in Leishmania
- Späth GF, Clos J
- Mol Microbiol doi: 100:923-927.10.1111/mmi.13374
A versatile qPCR assay to quantify trypanosomatidic infections of host cells and tissues
- Bifeld E, Tejera Nevado P, Bartsch J, Eick J, Clos J
- Med Microbiol Immunol. doi 10.1007/s00430-016-0460-3
A Telomeric Cluster of Antimony Resistance Genes on Chromosome 34 of Leishmania infantum
- Tejera Nevado P, Bifeld E, Höhn K, Clos J
- Antimicrob Agents Chemother. doi: 10.1128/AAC.00544-16
2015
Co-circulation of a novel phlebovirus and Massilia virus in sandflies, Portugal
- Amaro F, Zé-Zé L, Alves MJ, Börstler J, Clos J, Lorenzen S, Becker SC, Schmidt-Chanasit J, Cadar D
- Virol J. 2015 Oct 24;12:174. doi: 10.1186/s12985-015-0407-0
The genetics of Leishmania virulence
- Bifeld E, Clos J
- Med Microbiol Immunol. 2015 Dec;204(6):619-34. doi: 10.1007/s00430-015-0422-1
Geographical sequence variation in the Leishmania major virulence factor P46
- Bifeld E, Chrobak M, Zander D, Schleicher U, Schönian G, Clos J
- Infect Genet Evol. 2015 Mar;30:195-205. doi: 10.1016/j.meegid.2014.12.029
Leishmania donovani P23 protects parasites against HSP90 inhibitor-mediated growth arrest
- Hombach A, Ommen G, Sattler V, Clos J
- Cell Stress & Chaperones 2015, 20, 673-685
2014
Cyclophilin 40-deficient Leishmania donovani fail to undergo stress-induced development of the infectious metacyclic stage
- Yau WL, Pescher P, MacDonald A, Hem S, Zander D, Retzlaff S, Blisnick T, Rotureau B, Rosenqvist H, Wiese M, Bastin P, Clos J, Späth GF
- Mol Microbiol. 2014 Jul;93(1):80-97. doi: 10.1111/mmi.12639
Leishmania and the Leishmaniases: the heat shock protein 90 of Leishmania donovani
- Wiesgigl M, Clos J
- Parasitology. 2014 Aug;141(9):1156-66. doi: 10.1017/S0031182013002151
No stress - Hsp90 and the signal transduction in Leishmania
- Hombach A, Clos J
- Parasitology. 2014 Aug;141(9):1156-66. doi: 10.1017/S0031182013002151
Leishmania infantum EndoG is an endo/exo-nuclease essential for parasite survival
- Rico E, Oliva C, Gutierrez KJ, Alzate JF, Genes CM, Moreno D, Casanova E, Gigante A, Perez-Perez MJ, Camarasa MJ, Clos J, Gago F, Jimenez-Ruiz A
- PLoS One. 2014 Feb 26;9(2):e89526. doi: 10.1371/journal.pone.0089526
Reduced antimony accumulation in ARM58-overexpressing Leishmania infantum
- Schäfer C, Tejera Nevado P, Zander D, Clos J
- Antimicrob Agents Chemother. 2014;58(3):1565-74. doi: 10.1128/AAC.01881-13
Heat Shock Proteins of Leishmania: Parasites in the Driver's Seat
- Joachim Clos and Antje Hombach
- Adak, S., Datta, R. (Eds.), Leishmania – Current Biology and Control. Caister Academic Press, Norfolk, United Kingdom, pp. 17-36.
A Novel Marker, ARM58, Confers Antimony Resistance to Leishmania spp
- Nühs A, Schäfer C, Zander D, Trübe L, Tejera Nevado P, Schmidt S, Arevalo J, Adaui V, Maes L, Dujardin J-C, Clos J
- Int J Parasitol Drugs Drug Resist. 2013 Dec 6;4(1):37-47. doi: 10.1016/j.ijpddr.2013.11.004
A small heat shock protein is essential for thermotolerance and intracellular survival of Leishmania donovani
- Hombach A, Ommen G, MacDonald A, Clos J
- J. Cell Science (2014) in press doi: 10.1242/jcs.157297
2013
The loss of virulence of histone H1 overexpressing Leishmania donovani parasites is directly associated with a reduction of HSP83 rate of translation
- Alexandratos A, Clos J, Samiotaki M, Efstathiou A, Panayotou G, Soteriadou K, Smirlis D
- Mol Microbiol. 2013 Jun;88(5):1015-31. doi: 10.1111/mmi.12240
2012
The Hsp90-Sti1 Interaction is Critical for Leishmania donovani Proliferation in Both Life Cycle Stages
- Hombach A, Ommen G, Chrobak M, Clos J
- Cell Microbiol. 2013 Apr;15(4):585-600. doi: 10.1111/cmi.12057
Rapid identification of Leishmania spp. in formalin-fixed, paraffin-embedded tissue samples by fluorescence in situ hybridization
- Frickmann H, Alnamar Y, Essig A, Clos J, Racz P, Barth TF, Hagen RM, Fischer M, Poppert S
- Trop Med Int Health. 2012 Sep;17(9):1117-26. doi: 10.1111/j.1365-3156.2012.03024.x
Secreted virulence factors and immune evasion in visceral leishmaniasis
- Lambertz U, Silverman JM, Nandan D, McMaster WR, Clos J, Foster LJ, Reiner NE
- J Leukoc Biol. 2012 Jun;91(6):887-99. doi: 10.1189/jlb.0611326
Leishmania donovani HslV does not interact stably with HslU proteins
- Chrobak M, Forster S, Meisel S, Pfefferkorn R, Forster F, Clos J
- Int J Parasitol (2012) 42:329-339
2010
The co-chaperone SGT of Leishmania donovani is essential for the parasite's viability
- Ommen G, Chrobak M, Clos J
- Cell Stress Chaperones. 2010 Jul;15(4):443-55. doi: 10.1007/s12192-009-0160-7
Overexpression of a single Leishmania major gene is sufficient to enhance parasite infectivity in vivo and in vitro
- Reiling L, Chrobak M, Schmetz C, Clos J
- Mol Microbiol. 2010 Jun 1;76(5):1175-90. doi: 10.1111/j.1365-2958.2010.07130.x
Phosphoproteome dynamics reveals heat shock protein complexes specific to the Leishmania infectious stage
- Morales M, Watanabe R, Dacher M, Chafey P, Osorio y Fortéa J, Beverley S, Ommen G, Clos J, Hem S, Lenormand P, Rousselle J-C, Namane A, Spath G
- Proc Natl Acad Sci U S A. 2010 May 4;107(18):8381-6. doi: 10.1073/pnas.0914768107
LmxMPK4, an essential mitogen-activated protein kinase of Leishmania mexicana is phosphorylated and activated by the STE7-like protein kinase LmxMKK5
- von Freyend SJ, Rosenqvist H, Fink A, Melzer IM, Clos J, Jensen ON, Wiese M
- Int J Parasitol. 2010 Jul;40(8):969-78. doi: 10.1016/j.ijpara.2010.02.00
An exosome-based secretion pathway is responsible for protein export from Leishmania and communication with macrophages
- Silverman JM, Clos J, de'Oliveira CC, Shirvani O, Fang Y, Wang C, Foster LJ, Reiner NE
- J Cell Sci. 2010 Mar 15;123(Pt 6):842-52. doi: 10.1242/jcs.056465
Leishmania exosomes modulate innate and adaptive immune responses through effects on monocytes and dendritic cells
- Silverman JM, Clos J, Horakova E, Wang AY, Wiesgigl M, Kelly I, Lynn MA, McMaster WR, Foster LJ, Levings MK, Reiner NE
- J Immunol (2010) 185:5011-5022
Characterization of a Subunit of the Outer Dynein Arm Docking Complex Necessary for Correct Flagellar Assembly in Leishmania donovani
- Harder S, Thiel M, Clos J, Bruchhaus I
- PLoS Negl Trop Dis. 2010 Jan 26;4(1):e586. doi: 10.1371/journal.pntd.0000586
2009
One-step generation of double-allele gene replacement mutants in Leishmania donovani
- Ommen G, Lorenz S, Clos J
- Int J Parasitol. 2009 Apr;39(5):541-6. doi: 10.1016/j.ijpara.2008.10.009
Leishmania infantum expresses a mitochondrial nuclease homologous to EndoG that migrates to the nucleus in response to an apoptotic stimulus
- Rico E, Alzate JF, Arias AA, Moreno D, Clos J, Gago F, Moreno I, Dominguez M, Jimenez-Ruiz A
- Mol Biochem Parasitol. 2009 Jan;163(1):28-38. doi: 10.1016/j.molbiopara.2008.09.007
2008
Identification of a Leishmania infantum gene mediating resistance to miltefosine and SbIII
- Choudhury K, Zander D, Kube M, Reinhardt R, Clos J
- Int J Parasitol. 2008 Oct;38(12):1411-23. doi: 10.1016/j.ijpara.2008.03.005
Leishmania major: identification of developmentally regulated proteins in procyclic and metacyclic promastigotes
- Mojtahedi Z, Clos J, Kamali-Sarvestani E
- Exp Parasitol. 2008 Jul;119(3):422-9. doi: 10.1016/j.exppara.2008.04.008
2007
Structural characterization of beta-sheeted oligomers formed on the pathway of oxidative prion protein aggregation in vitro
- Redecke L, von Bergen M, Clos J, Konarev PV, Svergun DI, Fittschen UE, Broekaert JA, Bruns O, Georgieva D, Mandelkow E, Genov N, Betzel C
- J Struct Biol. 2007 Feb;157(2):308-20. doi: 10.1016/j.jsb.2006.06.01
2006
Spontaneous recovery of pathogenicity by Leishmania major hsp100-/- alters the immune response in mice
- Reiling L, Jacobs T, Kroemer M, Gaworski I, Graefe S, Clos J
- Infect Immun. 2006 Nov;74(11):6027-36. doi: 10.1128/IAI.00773-05
Functional cloning as a means to identify Leishmania genes involved in drug resistance
- Clos J, Choudhury K
- Mini Rev Med Chem. 2006 Feb;6(2):123-9. doi: 10.2174/138955706775476028
2005
Stage-specific expression of the mitochondrial co-chaperonin of Leishmania donovani, CPN10
- Zamora-Veyl FB, Kroemer M, Zander D, Clos J
- Kinetoplastid Biol Dis. 2005 Apr 29;4(1):3. doi: 10.1186/1475-9292-4-3
Comparative analysis of the human and chicken prion protein copper binding regions at pH 6.5
- Redecke L, Meyer-Klaucke W, Koker M, Clos J, Georgieva D, Genov N, Echner H, Kalbacher H, Perbandt M, Bredehorst R, Voelter W, Betzel C
- J Biol Chem. 2005 Apr 8;280(14):13987-92. doi: 10.1074/jbc.M411775200
2004
Synthetic human prion protein octapeptide repeat binds to the proteinase K active site
- Georgieva D, Rypniewski W, Echner H, Perbandt M, Koker M, Clos J, Redecke L, Bredehorst R, Voelter W, Genov N, Betzel C
- Biochem Biophys Res Commun. 2004 Dec 24;325(4):1406-11. doi: 10.1016/j.bbrc.2004.10.184
Oligomerization of the proteolytic products is an intrinsic property of prion proteins
- Georgieva D, Koker M, Redecke L, Perbandt M, Clos J, Bredehorst R, Genov N, Betzel C
- Biochem Biophys Res Commun. 2004 Oct 29;323(4):1278-86. doi: 10.1016/j.bbrc.2004.08.230
Complement C3 is required for the progression of cutaneous lesions and neutrophil attraction in Leishmania major infection
- Jacobs T, Andra J, Gaworski I, Graefe S, Mellenthin K, Kromer M, Halter R, Borlak J, Clos J
- Med Microbiol Immunol. 2005 May;194(3):143-9. doi: 10.1007/s00430-004-0229-y
A Leishmania donovani gene that confers accelerated recovery from stationary phase growth arrest
- Hoyer C, Zander D, Fleischer S, Schilhabel M, Kroener M, Platzer M, Clos J
- Int J Parasitol. 2004 Jun;34(7):803-11. doi: 10.1016/j.ijpara.2004.02.006
2003
Developmentally induced changes of the proteome in the protozoan parasite Leishmania donovani
- Bente M, Harder S, Wiesgigl M, Heukeshoven J, Gelhaus C, Krause E, Clos J, Bruchhaus I
- Proteomics. 2003 Sep;3(9):1811-29. doi: 10.1002/pmic.200300462
Comparison of the A2 gene locus in Leishmania donovani and Leishmania major and its control over cutaneous infection
- Zhang WW, Mendez S, Ghosh A, Myler P, Ivens A, Clos J, Sacks DL, Matlashewski G
- J Biol Chem. 2003 Sep 12;278(37):35508-15. doi: 10.1074/jbc.M305030200
2002
Inhibition of HSP90 in Trypanosoma cruzi Induces a Stress Response but No Stage Differentiation
- Graefe SE, Wiesgigl M, Gaworski I, Macdonald A, Clos J
- Eukaryot Cell. 2002 Dec;1(6):936-43. doi: 10.1128/EC.1.6.936-943.2002
2001
Heat shock protein 90 homeostasis controls stage differentiation in Leishmania donovani
- Wiesgigl M, Clos J
- Mol Biol Cell. 2001 Nov;12(11):3307-16. doi: 10.1091/mbc.12.11.3307
Leishmania and the Leishmaniases: Use of genetic complementation to identify gene(s) which specify species-specific organ tropism of Leishmania
- Hoyer C, Mellenthin K, Schilhabel M, Platzer M, Clos J
- Med Microbiol Immunol. 2001 Nov;190(1-2):43-6. doi: 10.1007/s004300100077
2000
Expression and Subcellular Localization of Cpn60 Protein Family Members in Leishmania donovani
- Schlüter A, Wiesgigl M, Hoyer C, Fleischer S, Klaholz L, Schmetz C, Clos J
- Biochim Biophys Acta. 2000 Apr 25;1491(1-3):65-74. doi: 10.1016/s0167-4781(00)00028-2
Cross-species Homologous Recombination in Leishmania donovani Reveals the Sites of Integration
- Krobitsch, S. and J. Clos
- Mol Biochem Parasitol. 2000 Mar 15;107(1):123-8. doi: 10.1016/s0166-6851(00)00180-8
1999
Uniform distribution of transcription complexes on the clpB gene locus of Leishmania donovani
- Wiesgigl M, Clos J
- Protist. 1999 Dec;150(4):369-73. doi: 10.1016/S1434-4610(99)70038-6
1998
Leishmania donovani heat shock protein 100: characterization and function in amastigote stage differentiation
- Krobitsch S, Brandau S, Hoyer C, Schmetz C, Hübel A, Clos J
- J Biol Chem. 1998 Mar 13;273(11):6488-94. doi: 10.1074/jbc.273.11.6488
1997
Leishmania major Hsp100 is required chiefly in the mammalian stage of the parasite
- Hübel A, Krobitsch S, Hörauf A, Clos J
- Mol Cell Biol. 1997 Oct;17(10):5987-95. doi: 10.1128/MCB.17.10.5987
1996
The genomic organization of the HSP83 gene locus is conserved in three Leishmania species
- Hübel A, Clos J
- Exp Parasitol. 1996 Mar;82(2):225-8. doi: 10.1006/expr.1996.0029
1995
A member of the ClpB family of stress proteins is expressed during heat shock in Leishmania spp
- Hübel A, Brandau S, Dresel A, Clos J
- Mol Biochem Parasitol. 1995 Mar;70(1-2):107-18. doi: 10.1016/0166-6851(95)00012-p
1994
pJC20 and pJC40- two high-copy-number vectors for T7 RNA polymerase-dependent expression of recombinant genes in Escherichia coli
- Clos J, Brandau S
- Protein Expr Purif. 1994 Apr;5(2):133-7. doi: 10.1006/prep.1994.1020