The microbiome of the marine flatworm Macrostomum lignano provides fitness advantages and exhibits circadian rhythmicity

Ma Y, He J, Sieber M, von Frieling J, Bruchhaus I, Baines JF, Bickmeyer U, Roeder T. 

Commun Biol. 2023 Mar 18;6(1):289. doi: 10.1038/s42003-023-04671-y. PMID: 36934156; PMCID: PMC10024726.


CD36-A Host Receptor Necessary for Malaria Parasites to Establish and Maintain Infection.

Bachmann A*, Metwally NG*, Allweier J, Cronshagen J, Del Pilar Martinez Tauler M, Murk A, Roth LK, Torabi H, Wu Y, Gutsmann T, Bruchhaus I.

Microorganisms. 2022 Nov 29;10(12):2356. doi: 10.3390/microorganisms10122356


An aqueous extract of the brown alga Eisenia bicyclis extends lifespan in a sex-specific manner by interfering with the Tor-FoxO axis.

Tahanzadeh N, Knop M, Seidler Y, Dirndorfer S, Lürsen K, Bruchhaus I, Lang R, Rimbach G, Roeder T. Aging (Albany NY). 2022 Aug 16;14(16):6427-6448. doi: 10.18632/aging.204218. Epub 2022 Aug 16.


Ectopic Expression of Plasmodium vivax vir Genes in P. falciparum Affects Cytoadhesion via Increased Expression of Specific var Genes.

Rehn T, Lubiana P, Nguyen THT, Pansegrau E, Schmitt M, Roth LK, Brehmer J, Roeder T, Cadar D, Metwally NG, Bruchhaus I. Microorganisms. 2022 Jun 9;10(6):1183. doi: 10.3390/microorganisms10061183.

HIF-1α modulates sex-specific Th17/Treg responses during hepatic amoebiasis.

Groneberg M, Hoenow S, Marggraff C, Fehling H, Metwally NG, Hansen C, Bruchhaus I, Tiegs G, Sellau J, Lotter H.

J Hepatol. 2022 Jan;76(1):160-173. doi: 10.1016/j.jhep.2021.09.020. Epub 2021 Sep 29. PMID: 34599999



An unusual trafficking domain in MSRP6 defines a complex needed for Maurer’s clefts anchoring and maintenance in P. falciparum infected red blood cells.

Blancke Soares A, Stäcker J, Schwald S, Hoijmakers W, Metwally NG, Schoeler H, Flemming S, Höhn K, Fröhlke U, Mesén-Ramírez P, Bergmann B, Khosh-Naucke M, Bruchhaus I, Bártfai R, Spielmann T. bioRxiv, 03 Dec 2021, doi: 10.1101/2021.12.03.471078 (DOI: 10.1101/2021.12.03.471078)


Anaerobic peroxisomes in Entamoeba histolytica metabolize myo-inositol.

Verner Z, Žárský V, Le T, Narayanasamy RK, Rada P, Rozbeský D, Makki A, Belišová D, Hrdý I, Vancová M, Lender C, König C, Bruchhaus I, Tachezy J.

PLoS Pathog. 2021 Nov 15;17(11):e1010041. doi: 10.1371/journal.ppat.1010041. eCollection 2021 Nov. PMID: 34780573


Altered Cytokine Response of Human Brain Endothelial Cells after Stimulation with Malaria Patient Plasma.

Raacke M, Kerr A, Dörpinghaus M, Brehmer J, Wu Y, Lorenzen S, Fink C, Jacobs T, Roeder T, Sellau J, Bachmann A, Metwally NG, Bruchhaus I.

Cells. 2021 Jul 1;10(7):1656. doi: 10.3390/cells10071656. PMID: 34359826


Analysis of the Interaction Between Plasmodium falciparum-Infected Erythrocytes and Human Endothelial Cells Using a Laminar Flow System, Bioinformatic Tracking and Transcriptome Analysis.

Wu Y, Bouws P, Lorenzen S, Bruchhaus I, Metwally NG.

Methods Mol Biol. 2021;2369:187-197. doi: 10.1007/978-1-0716-1681-9_11. PMID: 34313990


Common virulence gene expression in adult first-time infected malaria patients and severe cases.

Wichers JS, Tonkin-Hill G, Thye T, Krumkamp R, Kreuels B, Strauss J, von Thien H, Scholz JA, Smedegaard Hansson H, Weisel Jensen R, Turner L, Lorenz FR, Schöllhorn A, Bruchhaus I, Tannich E, Fendel R, Otto TD, Lavstsen T, Gilberger TW, Duffy MF, Bachmann A.

Elife. 2021 Apr 28;10:e69040. doi: 10.7554/eLife.69040. PMID: 33908865


Taxon-Specific Proteins of the Pathogenic Entamoeba Species E. histolytica and E. nuttalli.

König C, Honecker B, Wilson IW, Weedall GD, Hall N, Roeder T, Metwally NG, Bruchhaus I.

Front Cell Infect Microbiol. 2021 Mar 19;11:641472. doi: 10.3389/fcimb.2021.641472. eCollection 2021. PMID: 33816346



An Alcohol Dehydrogenase 3 (ADH3) from Entamoeba histolytica Is Involved in the Detoxification of Toxic Aldehydes.

König C, Meyer M, Lender C, Nehls S, Wallaschkowski T, Holm T, Matthies T, Lercher D, Matthiesen J, Fehling H, Roeder T, Reindl S, Rosenthal M, Metwally NG, Lotter H, Bruchhaus I. Microorganisms. 2020 Oct 19;8(10):1608. doi: 10.3390/microorganisms8101608. PMID: 33086693


Androgens predispose males to monocyte-mediated immunopathology by inducing the expression of leukocyte recruitment factor CXCL1.

Sellau J, Groneberg M, Fehling H, Thye T, Hoenow S, Marggraff C, Weskamm M, Hansen C, Stanelle-Bertram S, Kuehl S, Noll J, Wolf V, Metwally NG, Hagen SH, Dorn C, Wernecke J, Ittrich H, Tannich E, Jacobs T, Bruchhaus I, Altfeld M, Lotter H.

Nat Commun. 2020 Jul 10;11(1):3459. doi: 10.1038/s41467-020-17260-y. PMID: 32651360


Furbellow (Brown Algae) Extract Increases Lifespan in Drosophila by Interfering with TOR-Signaling.

Li Y, Romey-Glüsing R, Tahan Zadeh N, von Frieling J, Hoffmann J, Huebbe P, Bruchhaus I, Rimbach G, Fink C, Roeder T.

Nutrients. 2020 Apr 22;12(4):1172. doi: 10.3390/nu12041172. PMID: 32331413


Adhesion between P. falciparum infected erythrocytes and human endothelial receptors follows alternative binding dynamics under flow and febrile conditions.

Lubiana P, Bouws P, Roth LK, Dörpinghaus M, Rehn T, Brehmer J, Wichers JS, Bachmann A, Höhn K, Roeder T, Thye T, Gutsmann T, Burmester T, Bruchhaus I, Metwally NG.

Sci Rep. 2020 Mar 11;10(1):4548. doi: 10.1038/s41598-020-61388-2. PMID: 32161335


Stringent Selection of Knobby Plasmodium falciparum-Infected Erythrocytes during Cytoadhesion at Febrile Temperature.

Dörpinghaus M, Fürstenwerth F, Roth LK, Bouws P, Rakotonirinalalao M, Jordan V, Sauer M, Rehn T, Pansegrau E, Höhn K, Mesén-Ramírez P, Bachmann A, Lorenzen S, Roeder T, Metwally NG, Bruchhaus I.

Microorganisms. 2020 Jan 25;8(2):174. doi: 10.3390/microorganisms8020174. PMID: 31991814


Gene silencing and overexpression to study pathogenicity factors of Entamoeba histolytica. Eukaryome impact on human intestine homeostasis and mucosal immunology.

König C, Bruchhaus I

Nancy Guillen (Editor), Springer, 2020, ISBN 978-3-030-44825-7, p. 311-325, DOI 10.1007/978-3-030-44826-4



Dissecting the Gene Expression, Localization, Membrane Topology, and Function of the Plasmodium falciparum STEVOR Protein Family.

Wichers JS, Scholz JAM, Strauss J, Witt S, Lill A, Ehnold LI, Neupert N, Liffner B, Lühken R, Petter M, Lorenzen S, Wilson DW, Löw C, Lavazec C, Bruchhaus I, Tannich E, Gilberger TW, Bachmann A. mBio. 2019 Jul 30;10(4):e01500-19. doi: 10.1128/mBio.01500-19. PMID: 31363031


An EGFR-Induced Drosophila Lung Tumor Model Identifies Alternative Combination Treatments.

Bossen J, Uliczka K, Steen L, Pfefferkorn R, Mai MM, Burkhardt L, Spohn M, Bruchhaus I, Fink C, Heine H, Roeder T.

Mol Cancer Ther. 2019 Sep;18(9):1659-1668. doi: 10.1158/1535-7163.MCT-19-0168. Epub 2019 Jun 19. PMID: 31217165


Trigger-induced RNAi gene silencing to identify pathogenicity factors of Entamoeba histolytica.

Matthiesen J, Lender C, Haferkorn A, Fehling H, Meyer M, Matthies T, Tannich E, Roeder T, Lotter H, Bruchhaus I.

FASEB J. 2019 Feb;33(2):1658-1668. doi: 10.1096/fj.201801313R. Epub 2018 Aug 31. PMID: 30169111



Impaired Wnt signaling in dopamine containing neurons is associated with pathogenesis in a rotenone triggered Drosophila Parkinson's disease model.

Stephano F, Nolte S, Hoffmann J, El-Kholy S, von Frieling J, Bruchhaus I, Fink C, Roeder T.

Sci Rep. 2018 Feb 5;8(1):2372. doi: 10.1038/s41598-018-20836-w. PMID: 29403026


Nutritional regimens with periodically recurring phases of dietary restriction extend lifespan in Drosophila.

Romey-Glüsing R, Li Y, Hoffmann J, von Frieling J, Knop M, Pfefferkorn R, Bruchhaus I, Fink C, Roeder T.

FASEB J. 2018 Apr;32(4):1993-2003. doi: 10.1096/fj.201700934R. Epub 2018 Jan 5. PMID: 29196499



The Role of Monoaminergic Neurotransmission for Metabolic Control in the Fruit Fly Drosophila Melanogaster.

Li Y, Tiedemann L, von Frieling J, Nolte S, El-Kholy S, Stephano F, Gelhaus C, Bruchhaus I, Fink C, Roeder T.

Front Syst Neurosci. 2017 Aug 22;11:60. doi: 10.3389/fnsys.2017.00060. eCollection 2017. PMID: 28878633


Characterisation of Plasmodium falciparum populations selected on the human endothelial receptors P-selectin, E-selectin, CD9 and CD151.

Metwally NG, Tilly AK, Lubiana P, Roth LK, Dörpinghaus M, Lorenzen S, Schuldt K, Witt S, Bachmann A, Tidow H, Gutsmann T, Burmester T, Roeder T, Tannich E, Bruchhaus I.

Sci Rep. 2017 Jun 22;7(1):4069. doi: 10.1038/s41598-017-04241-3. PMID: 28642573



Octopamine controls starvation resistance, life span and metabolic traits in Drosophila.

Li Y, Hoffmann J, Li Y, Stephano F, Bruchhaus I, Fink C, Roeder T.

Sci Rep. 2016 Oct 19;6:35359. doi: 10.1038/srep35359. PMID: 27759117


Overexpression of Differentially Expressed Genes Identified in Non-pathogenic and Pathogenic Entamoeba histolytica Clones Allow Identification of New Pathogenicity Factors Involved in Amoebic Liver Abscess Formation.

Meyer M, Fehling H, Matthiesen J, Lorenzen S, Schuldt K, Bernin H, Zaruba M, Lender C, Ernst T, Ittrich H, Roeder T, Tannich E, Lotter H, Bruchhaus I.

PLoS Pathog. 2016 Aug 30;12(8):e1005853. doi: 10.1371/journal.ppat.1005853. eCollection 2016 Aug. PMID: 27575775


Social stress increases the susceptibility to infection in the ant Harpegnathos saltator.

Schneider SA, Scharffetter C, Wagner AE, Boesch C, Bruchhaus I, Rimbach G, Roeder T.

Sci Rep. 2016 May 10;6:25800. doi: 10.1038/srep25800. PMID: 27161621


IL-23 prevents IL-13-dependent tissue repair associated with Ly6C(lo) monocytes in Entamoeba histolytica-induced liver damage.

Noll J, Helk E, Fehling H, Bernin H, Marggraff C, Jacobs T, Huber S, Pelczar P, Ernst T, Ittrich H, Otto B, Mittrücker HW, Hölscher C, Tacke F, Bruchhaus I, Tannich E, Lotter H.

J Hepatol. 2016 May;64(5):1147-1157. doi: 10.1016/j.jhep.2016.01.013. Epub 2016 Jan 22. PMID: 26809113


Phylogeny of haemosporidian blood parasites revealed by a multi-gene approach.

Borner J, Pick C, Thiede J, Kolawole OM, Kingsley MT, Schulze J, Cottontail VM, Wellinghausen N, Schmidt-Chanasit J, Bruchhaus I, Burmester T.

Mol Phylogenet Evol. 2016 Jan;94(Pt A):221-31. doi: 10.1016/j.ympev.2015.09.003. Epub 2015 Sep 11. PMID: 26364971



Type of in vitro cultivation influences cytoadhesion, knob structure, protein localization and transcriptome profile of Plasmodium falciparum.

Tilly AK, Thiede J, Metwally N, Lubiana P, Bachmann A, Roeder T, Rockliffe N, Lorenzen S, Tannich E, Gutsmann T, Bruchhaus I.

Sci Rep. 2015 Nov 16;5:16766. doi: 10.1038/srep16766. PMID: 26568166


A comparative study of the localization and membrane topology of members of the RIFIN, STEVOR and PfMC-2TM protein families in Plasmodium falciparum-infected erythrocytes.

Bachmann A, Scholz JA, Janßen M, Klinkert MQ, Tannich E, Bruchhaus I, Petter M.

Malar J. 2015 Jul 16;14:274. doi: 10.1186/s12936-015-0784-2. PMID: 26173856


Magnetic resonance imaging of pathogenic protozoan parasite Entamoeba histolytica labeled with superparamagnetic iron oxide nanoparticles.

Ernst TM, Fehling H, Bernin H, Zaruba MD, Bruchhaus I, Adam G, Ittrich H, Lotter H.

Invest Radiol. 2015 Oct;50(10):709-18. doi: 10.1097/RLI.0000000000000175. PMID: 26135016


The Plasmodium falciparum exportome contains non-canonical PEXEL/HT proteins.

Schulze J, Kwiatkowski M, Borner J, Schlüter H, Bruchhaus I, Burmester T, Spielmann T, Pick C.

Mol Microbiol. 2015 Jul;97(2):301-14. doi: 10.1111/mmi.13024. Epub 2015 May 9. PMID: 25850860


Cysteine peptidases in pathogenesis. Amebiasis. Biology and Pathogenesis of Entamoeba.

Bruchhaus I, Matthiesen J.

Nozaki, Tomoyoshi, Bhattacharya, Alok (Eds.), Springer, 2015, ISBN 978-4-431-55199-7.



Transcriptional regionalization of the fruit fly's airway epithelium.

Faisal MN, Hoffmann J, El-Kholy S, Kallsen K, Wagner C, Bruchhaus I, Fink C, Roeder T.

PLoS One. 2014 Jul 14;9(7):e102534. doi: 10.1371/journal.pone.0102534. eCollection 2014. PMID: 25020150


Evidence of promiscuous endothelial binding by Plasmodium falciparum-infected erythrocytes.

Esser C, Bachmann A, Kuhn D, Schuldt K, Förster B, Thiel M, May J, Koch-Nolte F, Yáñez-Mó M, Sánchez-Madrid F, Schinkel AH, Jalkanen S, Craig AG, Bruchhaus I, Horstmann RD.

Cell Microbiol. 2014 May;16(5):701-8. doi: 10.1111/cmi.12270. Epub 2014 Feb 24. PMID: 24444337


The cell surface proteome of Entamoeba histolytica

Biller L, Matthiesen J, Kühne V, Lotter H, Handal G, Nozaki T, Saito-Nakano Y, Schümann M, Roeder T, Tannich E, Krause E, Bruchhaus I.

Mol Cell Proteomics. 2014 Jan;13(1):132-44. doi: 10.1074/mcp.M113.031393. Epub 2013 Oct 17. PMID: 24136294

Lophophorata monophyletic – after all. In


Deep Metazoan Phylogeny: Backbone of the Tree of Life.

Nesnidal MP, Helmkampf M, Bruchhaus I, Ebersberg I, Hausdorf, B.

Edited by Waegele JW, Bartholomaeus T, Misof B, Vogt L. Berlin: Walter de Gruyter; 2014..



New phylogenomic data support the monophyly of Lophophorata and an Ectoproct-Phoronid clade and indicate that Polyzoa and Kryptrochozoa are caused by systematic bias.


Nesnidal MP, Helmkampf M, Meyer A, Witek A, Bruchhaus I, Ebersberger I, Hankeln T, Lieb B, Struck TH, Hausdorf B.

BMC Evol Biol. 2013 Nov 17;13:253. doi: 10.1186/1471-2148-13-253. PMID: 24238092


Overexpression of specific cysteine peptidases confers pathogenicity to a nonpathogenic Entamoeba histolytica clone.

Matthiesen J, Bär AK, Bartels AK, Marien D, Ofori S, Biller L, Tannich E, Lotter H, Bruchhaus I.

mBio. 2013 Mar 26;4(2):e00072-13. doi: 10.1128/mBio.00072-13. PMID: 23532975

Agent of whirling disease meets orphan worm: phylogenomic analyses firmly place Myxozoa in Cnidaria.


Nesnidal MP, Helmkampf M, Bruchhaus I, El-Matbouli M, Hausdorf B.

PLoS One. 2013;8(1):e54576. doi: 10.1371/journal.pone.0054576. Epub 2013 Jan 30. PMID: 23382916



Temporal expression and localization patterns of variant surface antigens in clinical Plasmodium falciparum isolates during erythrocyte schizogony

Bachmann A, Petter M, Tilly AK, Biller L, Uliczka KA, Duffy MF, Tannich E, Bruchhaus I.

PLoS One. 2012;7(11):e49540. doi: 10.1371/journal.pone.0049540. Epub 2012 Nov 15. PMID: 23166704


Genomic diversity of the human intestinal parasite Entamoeba histolytica.

Weedall GD, Clark CG, Koldkjaer P, Kay S, Bruchhaus I, Tannich E, Paterson S, Hall N.

Genome Biol. 2012 May 25;13(5):R38. doi: 10.1186/gb-2012-13-5-r38. PMID: 22630046


The cysteine protease inhibitors EhICP1 and EhICP2 perform different tasks in the regulation of endogenous protease activity in trophozoites of Entamoeba histolytica.

Šarić M, Irmer H, Eckert D, Bär AK, Bruchhaus I, Scholze H.

Protist. 2012 Jan;163(1):116-28. doi: 10.1016/j.protis.2011.01.003. Epub 2011 Mar 25. PMID: 21440496


Entamoeba cysteine endopeptidases belonging to the C1 papain superfamily. In: Handbook of Proteolytic Enzymes, 3nd edition

Scholze H, Tannich E, Bruchhaus I.

Rawlings N.D. and Salvesen, G., eds., Ch. 438, Elsevier, 2012; pp. 1915-1918.



The complete mitochondrial genome of Flustra foliacea (Ectoprocta, Cheilostomata) - compositional bias affects phylogenetic analyses of lophotrochozoan relationships.

Nesnidal MP, Helmkampf M, Bruchhaus I, Hausdorf B.

BMC Genomics. 2011 Nov 23;12:572. doi: 10.1186/1471-2164-12-572. PMID: 22111761

Highly co-ordinated var gene expression and switching in clinical Plasmodium falciparum isolates from non-immune malaria patients.

Bachmann A, Predehl S, May J, Harder S, Burchard GD, Gilberger TW, Tannich E, Bruchhaus I.

Cell Microbiol. 2011 Sep;13(9):1397-409. doi: 10.1111/j.1462-5822.2011.01629.x. Epub 2011 Jul 11. PMID: 21740496


Phylogenomic analyses of malaria parasites and evolution of their exported proteins.

Pick C, Ebersberger I, Spielmann T, Bruchhaus I, Burmester T.

BMC Evol Biol. 2011 Jun 15;11:167. doi: 10.1186/1471-2148-11-167. PMID: 21676252



Compositional heterogeneity and phylogenomic inference of metazoan relationships.

Nesnidal MP, Helmkampf M, Bruchhaus I, Hausdorf B. Mol Biol Evol. 2010 Sep;27(9):2095-104. doi: 10.1093/molbev/msq097. Epub 2010 Apr 9. PMID: 20382658


The essentials of protein import in the degenerate mitochondrion of Entamoeba histolytica.

Dolezal P, Dagley MJ, Kono M, Wolynec P, Likić VA, Foo JH, Sedinová M, Tachezy J, Bachmann A, Bruchhaus I, Lithgow T.


Characterization of a subunit of the outer dynein arm docking complex necessary for correct flagellar assembly in Leishmania donovani.

Harder S, Thiel M, Clos J, Bruchhaus I.

PLoS Negl Trop Dis. 2010 Jan 26;4(1):e586. doi: 10.1371/journal.pntd.0000586. PMID: 20126266


Differences in the transcriptome signatures of two genetically related Entamoebahistolytica cell lines derived from the same isolate with different pathogenic properties.

Biller L, Davis PH, Tillack M, Matthiesen J, Lotter H, Stanley SL Jr, Tannich E, Bruchhaus I.

BMC Genomics. 2010 Jan 26;11:63. doi: 10.1186/1471-2164-11-63. PMID: 20102605


Phylogenetic relationships within the lophophorate lineages (Ectoprocta, Brachiopoda and Phoronida).

Hausdorf B, Helmkampf M, Nesnidal MP, Bruchhaus I.

Mol Phylogenet Evol. 2010 Jun;55(3):1121-7. doi: 10.1016/j.ympev.2009.12.022. Epub 2010 Jan 4. PMID: 20045074


tRNA gene sequences are required for transcriptional silencing in Entamoeba histolytica.

Irmer H, Hennings I, Bruchhaus I, Tannich E.

Eukaryot Cell. 2010 Feb;9(2):306-14. doi: 10.1128/EC.00248-09. Epub 2009 Dec 18. PMID: 20023072

Caenopores are antimicrobial peptides in the nematode Caenorhabditis elegans instrumental in nutrition and immunity.

Roeder T, Stanisak M, Gelhaus C, Bruchhaus I, Grötzinger J, Leippe M.

Dev Comp Immunol. 2010 Feb;34(2):203-9. doi: 10.1016/j.dci.2009.09.010. Epub 2009 Oct 21. PMID: 19818806



Absence of erythrocyte sequestration and lack of multicopy gene family expression in Plasmodium falciparum from a splenectomized malaria patient.

Bachmann A, Esser C, Petter M, Predehl S, von Kalckreuth V, Schmiedel S, Bruchhaus I, Tannich E.

PLoS One. 2009 Oct 14;4(10):e7459. doi: 10.1371/journal.pone.0007459. PMID: 19826486


Comparison of two genetically related Entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties.

Biller L, Schmidt H, Krause E, Gelhaus C, Matthiesen J, Handal G, Lotter H, Janssen O, Tannich E, Bruchhaus I.

Proteomics. 2009 Sep;9(17):4107-20. doi: 10.1002/pmic.200900022. PMID: 19688750


Major cysteine peptidases of Entamoeba histolytica are required for aggregation and digestion of erythrocytes but are dispensable for phagocytosis and cytopathogenicity.

Irmer H, Tillack M, Biller L, Handal G, Leippe M, Roeder T, Tannich E, Bruchhaus I.

Mol Microbiol. 2009 May;72(3):658-67. doi: 10.1111/j.1365-2958.2009.06672.x. PMID: 19426210



An Entamoeba cysteine peptidase specifically expressed during encystation.

Ebert F, Bachmann A, Nakada-Tsukui K, Hennings I, Drescher B, Nozaki T, Tannich E, Bruchhaus I.

Parasitol Int. 2008 Dec;57(4):521-4. doi: 10.1016/j.parint.2008.07.002. Epub 2008 Aug 5. PMID: 18723116


Phylogenomic analyses of lophophorates (brachiopods, phoronids and bryozoans) confirm the Lophotrochozoa concept.

Helmkampf M, Bruchhaus I, Hausdorf B.

Proc Biol Sci. 2008 Aug 22;275(1645):1927-33. doi: 10.1098/rspb.2008.0372. PMID: 18495619


Involvement of a Leishmania thymidine kinase in flagellum formation, promastigote shape and growth as well as virulence.

Thiel M, Harder S, Wiese M, Kroemer M, Bruchhaus I.

Mol Biochem Parasitol. 2008 Apr;158(2):152-62. doi: 10.1016/j.molbiopara.2007.12.005. Epub 2007 Dec 15. PMID: 18222009


Characterization of a conserved rhoptry-associated leucine zipper-like protein in the malaria parasite Plasmodium falciparum.

Haase S, Cabrera A, Langer C, Treeck M, Struck N, Herrmann S, Jansen PW, Bruchhaus I, Bachmann A, Dias S, Cowman AF, Stunnenberg HG, Spielmann T, Gilberger TW.

Infect Immun. 2008 Mar;76(3):879-87. doi: 10.1128/IAI.00144-07. Epub 2008 Jan 3. PMID: 18174339

Multigene analysis of lophophorate and chaetognath phylogenetic relationships.

Helmkampf M, Bruchhaus I, Hausdorf B.

Mol Phylogenet Evol. 2008 Jan;46(1):206-14. doi: 10.1016/j.ympev.2007.09.004. Epub 2007 Sep 12. PMID: 17937996



Structure and content of the Entamoeba histolytica genome.

Clark CG, Alsmark UC, Tazreiter M, Saito-Nakano Y, Ali V, Marion S, Weber C, Mukherjee C, Bruchhaus I, Tannich E, Leippe M, Sicheritz-Ponten T, Foster PG, Samuelson J, Noël CJ, Hirt RP, Embley TM, Gilchrist CA, Mann BJ, Singh U, Ackers JP, Bhattacharya S, Bhattacharya A, Lohia A, Guillén N, Duchêne M, Nozaki T, Hall N.

Adv Parasitol. 2007;65:51-190. doi: 10.1016/S0065-308X(07)65002-7. PMID: 18063096


Spiralian phylogenomics supports the resurrection of Bryozoa comprising Ectoprocta and Entoprocta.

Hausdorf B, Helmkampf M, Meyer A, Witek A, Herlyn H, Bruchhaus I, Hankeln T, Struck TH, Lieb B.

Mol Biol Evol. 2007 Dec;24(12):2723-9. doi: 10.1093/molbev/msm214. Epub 2007 Oct 5. PMID: 17921486


Protozoan parasites: programmed cell death as a mechanism of parasitism.

Bruchhaus I, Roeder T, Rennenberg A, Heussler VT.

Trends Parasitol. 2007 Aug;23(8):376-83. doi: 10.1016/j.pt.2007.06.004. Epub 2007 Jun 27. PMID: 17588817 Review.


The Entamoeba histolytica genome: primary structure and expression of proteolytic enzymes.

Tillack M, Biller L, Irmer H, Freitas M, Gomes MA, Tannich E, Bruchhaus I.

BMC Genomics. 2007 Jun 14;8:170. doi: 10.1186/1471-2164-8-170. PMID: 17567921


Proteomic analysis of Entamoeba histolytica.

Tolstrup J, Krause E, Tannich E, Bruchhaus I.

Parasitology. 2007 Feb;134(Pt 2):289-98. doi: 10.1017/S0031182006001442. Epub 2006 Oct 11. PMID: 17032470



The second cysteine protease inhibitor, EhICP2, has a different localization in trophozoites of Entamoeba histolytica than EhICP1.

Sarić M, Vahrmann A, Bruchhaus I, Bakker-Grunwald T, Scholze H.

Parasitol Res. 2006 Dec;100(1):171-4. doi: 10.1007/s00436-006-0206-z. Epub 2006 Jun 27. PMID: 16802137


Increased expression of the major cysteine proteinases by stable episomal transfection underlines the important role of EhCP5 for the pathogenicity of Entamoeba histolytica.

Tillack M, Nowak N, Lotter H, Bracha R, Mirelman D, Tannich E, Bruchhaus I.

Mol Biochem Parasitol. 2006 Sep;149(1):58-64. doi: 10.1016/j.molbiopara.2006.04.009. Epub 2006 May 19. PMID: 16753229


Expression of a mitochondrial peroxiredoxin prevents programmed cell death in Leishmania donovani.

Harder S, Bente M, Isermann K, Bruchhaus I. Eukaryot Cell. 2006 May;5(5):861-70. doi: 10.1128/EC.5.5.861-870.2006. PMID: 16682463



Identification of EhICP1, a chagasin-like cysteine protease inhibitor of Entamoeba histolytica.

Riekenberg S, Witjes B, Sarić M, Bruchhaus I, Scholze H. FEBS Lett. 2005 Mar 14;579(7):1573-8. doi: 10.1016/j.febslet.2005.01.067. PMID: 15757643


The genome of the protist parasite Entamoeba histolytica.

Loftus B, Anderson I, Davies R, Alsmark UC, Samuelson J, Amedeo P, Roncaglia P, Berriman M, Hirt RP, Mann BJ, Nozaki T, Suh B, Pop M, Duchene M, Ackers J, Tannich E, Leippe M, Hofer M, Bruchhaus I, Willhoeft U, Bhattacharya A, Chillingworth T, Churcher C, Hance Z, Harris B, Harris D, Jagels K, Moule S, Mungall K, Ormond D, Squares R, Whitehead S, Quail MA, Rabbinowitsch E, Norbertczak H, Price C, Wang Z, Guillén N, Gilchrist C, Stroup SE, Bhattacharya S, Lohia A, Foster PG, Sicheritz-Ponten T, Weber C, Singh U, Mukherjee C, El-Sayed NM, Petri WA Jr, Clark CG, Embley TM, Barrell B, Fraser CM, Hall N.

Nature. 2005 Feb 24;433(7028):865-8. doi: 10.1038/nature03291. PMID: 15729342


In vitro amoebicidal activity of some medicinal plants of the Bambun region (Cameroon)

Moundipa PF, Melanie Flore KG, Bilong Bilong CF, Bruchhaus, I.

Afr. J. Trad. CAM. 2005;2:113-121



Resistance of Entamoeba histolytica to the cysteine proteinase inhibitor E64 is associated with secretion of pro-enzymes and reduced pathogenicity.

Nowak N, Lotter H, Tannich E, Bruchhaus I.

J Biol Chem. 2004 Sep 10;279(37):38260-6. doi: 10.1074/jbc.M405308200. Epub 2004 Jun 23. PMID: 15215238


A peroxiredoxin specifically expressed in two types of pharyngeal neurons is required for normal growth and egg production in Caenorhabditis elegans.

Isermann K, Liebau E, Roeder T, Bruchhaus I.

J Mol Biol. 2004 May 7;338(4):745-55. doi: 10.1016/j.jmb.2004.03.021. PMID: 15099742


Entamoeba histolytica trophozoites transfer lipophosphopeptidoglycans to enteric cell layers.

Lauwaet T, Oliveira MJ, De Bruyne G, Bruchhaus I, Duchêne M, Mareel M, Leroy A.

Int J Parasitol. 2004 Apr;34(5):549-56. doi: 10.1016/j.ijpara.2003.11.013. PMID: 15064119


Effects of bisphosphonates on the growth of Entamoeba histolytica and Plasmodium species in vitro and in vivo.

Ghosh S, Chan JM, Lea CR, Meints GA, Lewis JC, Tovian ZS, Flessner RM, Loftus TC, Bruchhaus I, Kendrick H, Croft SL, Kemp RG, Kobayashi S, Nozaki T, Oldfield E.

J Med Chem. 2004 Jan 1;47(1):175-87. doi: 10.1021/jm030084x. PMID: 14695831



Developmentally induced changes of the proteome in the protozoan parasite Leishmania donovani.

Bente M, Harder S, Wiesgigl M, Heukeshoven J, Gelhaus C, Krause E, Clos J, Bruchhaus I.

Proteomics. 2003 Sep;3(9):1811-29. doi: 10.1002/pmic.200300462. PMID: 12973740

The intestinal protozoan parasite Entamoeba histolytica contains 20 cysteine protease genes, of which only a small subset is expressed during in vitro cultivation.

Bruchhaus I, Loftus BJ, Hall N, Tannich E.

Eukaryot Cell. 2003 Jun;2(3):501-9. doi: 10.1128/EC.2.3.501-509.2003. PMID: 12796295



Differential gene expression in Entamoeba histolytica isolated from amoebic liver abscess.

Bruchhaus I, Roeder T, Lotter H, Schwerdtfeger M, Tannich E.

Mol Microbiol. 2002 May;44(4):1063-72. doi: 10.1046/j.1365-2958.2002.02941.x. PMID: 12010498


Recombinant expression and purification of an enzymatically active cysteine proteinase of the protozoan parasite Entamoeba histolytica.

Hellberg A, Nowak N, Leippe M, Tannich E, Bruchhaus I.

Protein Expr Purif. 2002 Feb;24(1):131-7. doi: 10.1006/prep.2001.1548. PMID: 11812234


Virtual northern blot. Analysing gene expression. A handbook of methods possibilities and pitfalls.

Roeder T, Bruchhaus I.

Edited by Stefan Lorkowski and Paul Cullen, Wiley-VCH, 2002; ISBN 3-527-30488-6, 260-266.



Comparative proteome analysis of Leishmania donovani at different stages of transformation from promastigotes to amastigotes.

Thiel M, Bruchhaus I.

Med Microbiol Immunol. 2001 Nov;190(1-2):33-6. doi: 10.1007/s004300100075. PMID: 11770106


Introns of Entamoeba histolytica and Entamoeba dispar.

Wilihoeft U, Campos-Góngora E, Touzni S, Bruchhaus I, Tannich E.

Protist. 2001 Jul;152(2):149-56. doi: 10.1078/1434-4610-00053. PMID: 11545438


Overexpression of cysteine proteinase 2 in Entamoeba histolytica or Entamoeba dispar increases amoeba-induced monolayer destruction in vitro but does not augment amoebic liver abscess formation in gerbils.

Hellberg A, Nickel R, Lotter H, Tannich E, Bruchhaus I.

Cell Microbiol. 2001 Jan;3(1):13-20. doi: 10.1046/j.1462-5822.2001.00086.x. PMID: 11207616



A simple and reliable 5'-RACE approach.

Schramm G, Bruchhaus I, Roeder T.

Nucleic Acids Res. 2000 Nov 15;28(22):E96. doi: 10.1093/nar/28.22.e96. PMID: 11071950


Superoxide dismutase reduces susceptibility to metronidazole of the pathogenic protozoan Entamoeba histolytica under microaerophilic but not under anaerobic conditions.

Wassmann C, Bruchhaus I.

Arch Biochem Biophys. 2000 Apr 1;376(1):236-8. doi: 10.1006/abbi.2000.1707. PMID: 10729211


Two major 'higher molecular mass proteinases' of Entamoeba histolytica are identified as cysteine proteinases 1 and 2.

Hellberg A, Leippe M, Bruchhaus I.

Mol Biochem Parasitol. 2000 Feb 5;105(2):305-9. doi: 10.1016/s0166-6851(99)00194-2. PMID: 10693753



Metronidazole resistance in the protozoan parasite Entamoeba histolytica is associated with increased expression of iron-containing superoxide dismutase and peroxiredoxin and decreased expression of ferredoxin 1 and flavin reductase.

Wassmann C, Hellberg A, Tannich E, Bruchhaus I.

J Biol Chem. 1999 Sep 10;274(37):26051-6. doi: 10.1074/jbc.274.37.26051. PMID: 10473552


Verification of differential gene transcription using virtual northern blotting.

Franz O, Bruchhaus I, Roeder T.

Nucleic Acids Res. 1999 Jun 1;27(11):e3. doi: 10.1093/nar/27.11.e3. PMID: 10325436


Primary sequence of a putative non-ATPase subunit of the 26S proteasome from Entamoeba histolytica is similar to the human and yeast S2 subunit.

Hellberg A, Sommer A, Bruchhaus I.

Parasitol Res. 1999 May;85(5):417-20. doi: 10.1007/s004360050569. PMID: 10227061



Isolation of ultrapure supercoiled plasmid-DNA using preparative electrophoresis.

Roeder T, Görich D, Roeder NP, Bruchhaus I.

Electrophoresis. 1998 Jul;19(10):1575-6. doi: 10.1002/elps.1150191009. PMID: 9719528


Recombinant expression and biochemical characterization of an NADPH:flavin oxidoreductase from Entamoeba histolytica.

Bruchhaus I, Richter S, Tannich E.

Biochem J. 1998 Mar 15;330 ( Pt 3)(Pt 3):1217-21. doi: 10.1042/bj3301217. PMID: 9494088


Isolation and molecular characterization of a surface-bound proteinase of Entamoeba histolytica.

Jacobs T, Bruchhaus I, Dandekar T, Tannich E, Leippe M.

Mol Microbiol. 1998 Jan;27(2):269-76. doi: 10.1046/j.1365-2958.1998.00662.x. PMID: 9484883



Removal of hydrogen peroxide by the 29 kDa protein of Entamoeba histolytica.

Bruchhaus I, Richter S, Tannich E.

Biochem J. 1997 Sep 15;326 ( Pt 3)(Pt 3):785-9. doi: 10.1042/bj3260785. PMID: 9307028


Characterization of two E. histolytica proteins that inactivate reactive oxygen species.

Bruchhaus I, Richter S, Tannich E.

Arch Med Res. 1997;28 Spec No:91-2. PMID: 9033025



Entamoeba histolytica and Entamoeba dispar: differences in numbers and expression of cysteine proteinase genes.

Bruchhaus I, Jacobs T, Leippe M, Tannich E.

Mol Microbiol. 1996 Oct;22(2):255-63. doi: 10.1046/j.1365-2958.1996.00111.x. PMID: 8930910


Pyrophosphate-dependent phosphofructokinase of Entamoeba histolytica: molecular cloning, recombinant expression and inhibition by pyrophosphate analogues.

Bruchhaus I, Jacobs T, Denart M, Tannich E.

Biochem J. 1996 May 15;316 ( Pt 1)(Pt 1):57-63. doi: 10.1042/bj3160057. PMID: 8645233


A gene highly homologous to ACP1 encoding cysteine proteinase 3 in Entamoeba histolytica is present and expressed in E. dispar.

Bruchhaus I, Tannich E.

Parasitol Res. 1996;82(2):189-92. doi: 10.1007/s004360050093. PMID: 8825217



Identification of an Entamoeba histolytica gene encoding a protein homologous to prokaryotic disulphide oxidoreductases.

Bruchhaus I, Tannich E.

Mol Biochem Parasitol. 1995 Mar;70(1-2):187-91. doi: 10.1016/0166-6851(94)00214-8. PMID: 7637700

Free radicals and protozoa

Bruchhaus I, Dive D.

Protistol Actual. 1995, 195-203.



Purification and molecular characterization of the NAD(+)-dependent acetaldehyde/alcohol dehydrogenase from Entamoeba histolytica.

Bruchhaus I, Tannich E.

Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):743-8. doi: 10.1042/bj3030743. PMID: 7980441


Induction of the iron-containing superoxide dismutase in Entamoeba histolytica by a superoxide anion-generating system or by iron chelation.

Bruchhaus I, Tannich E.

Mol Biochem Parasitol. 1994 Oct;67(2):281-8. doi: 10.1016/0166-6851(94)00143-x. PMID: 7870132



Unusual gene organization in the protozoan parasite Entamoeba histolytica.

Bruchhaus I, Leippe M, Lioutas C, Tannich E.

DNA Cell Biol. 1993 Dec;12(10):925-33. doi: 10.1089/dna.1993.12.925. PMID: 8274224


Primary structure of the pyruvate phosphate dikinase in Entamoeba histolytica.

Bruchhaus I, Tannich E.

Mol Biochem Parasitol. 1993 Nov;62(1):153-6. doi: 10.1016/0166-6851(93)90193-2. PMID: 8114821


Analysis of the genomic sequence encoding the 29-kDa cysteine-rich protein of Entamoeba histolytica.

Bruchhaus I, Tannich E.

Trop Med Parasitol. 1993 Jun;44(2):116-8. PMID: 8367659



Purification of glutathione reductase from muscle of the adult parasitic nematode Ascaris suum.

Komuniecki R, Bruchhaus I, Ilg T, Wilson K, Zhang Y, Fairlamb AH.

Mol Biochem Parasitol. 1992 Apr;51(2):331-3. doi: 10.1016/0166-6851(92)90084-w. PMID: 1574090


Recombinant expression, purification and biochemical characterization of a superoxide dismutase from Entamoeba histolytica.

Bruchhaus I, Brattig NW, Tannich E.

Arch Med Res. 1992;23(2):27-9. PMID: 1340312



Pathogenic and nonpathogenic Entamoeba histolytica: identification and molecular cloning of an iron-containing superoxide dismutase.

Tannich E, Bruchhaus I, Walter RD, Horstmann RD.

Mol Biochem Parasitol. 1991 Nov;49(1):61-71. doi: 10.1016/0166-6851(91)90130-x. PMID: 1775159











AG Wirt-Parasit-Interaktion

Protrait einer sympathischen Frau mit kurzern braunen Haaren lächelt freundlich vor einem Rapsfeld.
Research Group Leader

Prof. Dr. Iris Bruchhaus

Telefon: +49 40 285380-472

E-Mail: bruchhaus@bnitm.de

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