Pathogen detection in ancient DNA
Molecular pathogen detection in archived samples
The identification of (novel) pathogens, especially for the restrospective detection in past outbreaks, is often restrained by the absence of stored serum or cerebrospinal fluid samples. However, from such epidemics, case series, or single cases of unknown etiology, formalin-fixed paraffin-embedded samples sometimes remain in pathology archives for retrospective molecular analysis. The past decade has seen a steady increase in the appilcability of NGS for the detection and characterization of pathogens also from such archived tissue samples. Nucleic acids recovered from these clinical specimens that were not stored specifically for later DNA (and RNA) analyses may provide insights not only in the cause of a past outbreak, but also in pathogen evolution.
No metagenomic evidence of causative viral pathogens in postencephalitic parkinsonism following encephalitis lethargica.
- Cadar D, Jellinger KA, Riederer P, Strobel S, Monoranu CM, Tappe D.
- Microorganisms. 2021 Aug 12;9(8):1716.
Rickettsia typhi as cause of fatal encephalitic typhus in hospitalized patients, Germany, 1940-1944.
- Rauch J, Muntau B, Eggert P, Tappe D.
- Emerg Infect Dis. 2018 Nov;24(11):1982-1987.
Medical and molecular perspectives into a forgotten epidemic: encephalitis lethargica, viruses, and high-throughput sequencing.
- Tappe D, Alquezar-Planas DE.
- J Clin Virol. 2014 Oct;61(2):189-95