Arbeitgruppe: Kichwa de Sarayaku, Amazonian rainforest, Ecuador
Kichwa de Sarayaku, Amazonian rainforest, Ecuador

OVERVIEW

NGS Core Facility

The NGS Core Facility is a recently established and integrated unit of the BNITM which provides its NGS support to all research groups of the Institute and external collaborators. The Core Facility operates several next-generation sequencers using sequencing-by-synthesis technology from Illumina, including NextSeq2000, NextSeq550, MiSeq and iSeq100, and maintains a fully equipped lab and staff able to perform a variety of sequencing applications - from sample preparation to data analysis. The NGS Facility was founded to help researchers process DNA and RNA samples in an efficient and economical manner. We have established several workflows and pipelines for the sample preparation to increase the throughput and minimize technical variation. By centralizing equipment and expertise, we have dramatically reduced the overall expense to the scientific community, while increasing the efficiency and quality of the data generated. Preparation of libraries for various applications is supported by a robust instrumentation infrastructure and closed system laboratory (e.g. Covaris, Bioanalyzer, Qubit, Pippin Prep, qPCR, and more). The NGS Core provides several analyses in a single- or a pair-end sequencing mode, including multiplexing libraries:

-        Viral metagenomics and metatranscriptomics: RNA-Seq/DNA-Seq

-        Genome-wide location analysis of nucleic acid-protein interactions - ChIP-Seq, CLIP-Seq

-        Whole Transcriptome sequencing: RNA-Seq

-        Insects genome sequencing

-        Discovery of small non-coding RNAs: ncRNA-Seq.

-        Genome-wide DNA methylation analysis: Methyl/BS-Seq.

-        De novo sequencing and re-sequencing of genomic DNA.

-        Targeted enrichment and sequencing (sequence capture).

-        Single-cell genomic applications (scRNA-Seq, scDNA-Seq)

-        16S Metagenomic Sequencing

-        miRNA and Small RNA Analysis

-        Long-Range Amplicon Sequencing

 

Example of metagenomic pipeline developed for virus discovery

Research Projects

Preparedness and Response in an Emergency context to Pathogens of MEDical and VETerinary importance

The rapid advances in sequencing and computational technologies accompanied by a drastic reduction of sequencing costs, have revolutionized microbial genomics and provided an unprecedented insight into microbial evolution, diversity, and host–pathogen interaction. Moreover, genomic technologies demonstrated great potential and suitability for clinical diagnostics or the real-time detection and surveillance of epidemics. The overall goal of the PREPMedVet is to set up a portable diagnostic sequencing platform (iSeq100, Illumina) capable to deliver in a clinically relevant turnaround time critical information in the context of an outbreak. The consortium has aimed to develop a standard wet-lab protocol which could be applied on-site using portable next-generation sequencing platforms and user-friendly sequence analysis software’s capable to deliver valuable insight into epidemic transmission patterns, pathogen genomics, evolution and the possible source of origin. The identification of pathogens will be flanked by production and validation of easy-to-use detection assays that can be stockpiled, transported, and delivered to the end users for timely and comprehensive reaction on outbreak scenarios.

Publications

2022

Causes of fever in returning TRAVELERS: A EUROPEAN MULTICENTER prospective cohort study
Daniel Camprubí-Ferrer, Ludovico Cobuccio, Steven Van Den Broucke, Blaise Genton, Emmanuel Bottieau, Valérie d'Acremont, Natalia Rodriguez-Valero, Alex Almuedo-Riera, Leire Balerdi-Sarasola, Carme Subirà, Marc Fernandez-Pardos, Miguel J Martinez, Jessica Navero-Castillejos, Isabel Vera, Jara Llenas-Garcia, Camilla Rothe, Dániel Cadar, Marjan Van Esbroeck, Nikki Foque, Jose Muñoz
Journal of Travel Medicine, taac002, https://doi.org/10.1093/jtm/taac002

2021

Differential neurovirulence of Usutu virus lineages in mice and neuronal cells.
Clé M, Constant O, Barthelemy J, Desmetz C, Martin MF, Lapeyre L, Cadar D, Savini G, Teodori L, Monaco F, Schmidt-Chanasit J, Saiz JC, Gonzales G, Lecollinet S, Beck C, Gosselet F, Van de Perre P, Foulongne V, Salinas S, Simonin Y.
J Neuroinflammation. 2021 Jan 6;18(1):11. doi: 10.1186/s12974-020-02060-4.

Genome Sequences of West Nile Virus Reference Materials.
Roth H, Kreß J, Chudy M, Blümel J, Schmidt-Chanasit J, Cadar D, Niedrig M, Ferenczi E, Herrmann M, Baylis SA, Miskey C.
Microbiol Resour Announc. 2021 Oct 28;10(43):e0074021. doi: 10.1128/MRA.00740-21. Epub 2021 Oct 28

Phylogeny of Hungarian EBLV-1 strains using whole-genome sequence data.
Forró B, Marton S, Fehér E, Domán M, Kemenesi G, Cadar D, Hornyák Á, Bányai K.
Transbound Emerg Dis. 2021 May;68(3):1323-1331. doi: 10.1111/tbed.13789

Genomic surveillance of SARS-CoV-2 in the Republic of Congo.
Ntoumi F, Mfoutou Mapanguy CC, Tomazatos A, Pallerla SR, Linh LTK, Casadei N, Angelov A, Sonnabend M, Peter S, Kremsner PG, Velavan TP
Int J Infect Dis. 2021 Apr;105:735-738. doi: 10.1016/j.ijid.2021.03.036.

Predominance of HBV Genotype B and HDV Genotype 1 in Vietnamese Patients with Chronic Hepatitis
Hoan NX, Hoechel M, Tomazatos A, Anh CX, Pallerla SR, Linh LTK, Binh MT, Sy BT, Toan NL, Wedemeyer H, Bock CT, Kremsner PG, Meyer CG, Song LH, Velavan TP.
Predominance of HBV Genotype B and HDV Genotype 1 in Vietnamese Patients with Chronic Hepatitis. Hoan NX, Hoechel M, Tomazatos A, Anh CX, Pallerla SR, Linh LTK, Binh MT, Sy BT, Toan NL, Wedemeyer H, Bock CT, Kremsner PG, Meyer CG, Song LH, Velavan TP. Viruses. 2021 Feb 22;13(2):346. doi: 10.3390/v13020346

Molecular surveillance and genetic divergence of rotavirus A antigenic epitopes in Gabonese children with acute gastroenteritis
Manouana GP, Niendorf S, Tomazatos A, Mbong Ngwese M, Nzamba Maloum M, Nguema Moure PA, Bingoulou Matsougou G, Ategbo S, Rossatanga EG, Bock CT, Borrmann S, Mordmüller B, Eibach D, Kremsner PG, Velavan TP, Adegnika AA
EBioMedicine. 2021 Oct 24;73:103648. doi: 10.1016/j.ebiom.2021.103648

Taxonomic update for mammalian anelloviruses (family Anelloviridae).
Varsani A, Opriessnig T, Celer V, Maggi F, Okamoto H, Blomström AL, Cadar D, Harrach B, Biagini P, Kraberger S.
Arch Virol. 2021 Oct;166(10):2943-2953. doi: 10.1007/s00705-021-05192-x.

Discovery and genetic characterization of a novel orthonairovirus in Ixodes ricinus ticks from Danube Delta
Tomazatos A, von Possel R, Pekarek N, Holm T, Rieger T, Baum H, Bialonski A, Maranda I, Erdelyi-Molnár I, Spînu M, Lühken R, Jansen S, Emmerich P, Schmidt-Chanasit J, Cadar D.
Infect Genet Evol. 2021 Mar;88:104704. doi: 10.1016/j.meegid.2021.104704

Metagenomic Snapshots of Viral Components in Guinean Bats.
Hermida Lorenzo RJ, Cadar D, Koundouno FR, Juste J, Bialonski A, Baum H, García-Mudarra JL, Hakamaki H, Bencsik A, Nelson EV, Carroll MW, Magassouba N, Günther S, Schmidt-Chanasit J, Muñoz Fontela C, Escudero-Pérez B
Microorganisms. 2021 Mar 15;9(3):599. doi: 10.3390/microorganisms9030599

West-Nile-Virus Infection acquired in Germany in a Kidney Transplant Recipient
Karrasch M, Pein U, Fritz A, Lange D, Moritz S, Amann K, Schmidt-Chanasit J, Cadar D, Tappe D, Gabriel M.
[West-Nile-Virus Infection acquired in Germany in a Kidney Transplant Recipient]. Karrasch M, Pein U, Fritz A, Lange D, Moritz S, Amann K, Schmidt-Chanasit J, Cadar D, Tappe D, Gabriel M. Dtsch Med Wochenschr. 2021 Apr;146(7):482-486. doi: 10.1055/a-1218-9096

Genomic and Micro-Evolutionary Features of Mammalian 2 orthobornavirus (Variegated Squirrel Bornavirus 1, VSBV-1)
Cadar D, Schmidt-Chanasit J, Tappe D
Microorganisms. 2021 May 25;9(6):1141. doi: 10.3390/microorganisms9061141

No Metagenomic Evidence of Causative Viral Pathogens in Postencephalitic Parkinsonism Following Encephalitis Lethargica
Cadar D, Jellinger KA, Riederer P, Strobel S, Monoranu CM, Tappe D
Microorganisms. 2021 Aug 12;9(8):1716. doi: 10.3390/microorganisms9081716

Taxonomic updates for the genus Gyrovirus (family Anelloviridae): recognition of several new members and establishment of species demarcation criteria
Kraberger S, Opriessnig T, Celer V, Maggi F, Okamoto H, Blomström AL, Cadar D, Harrach B, Biagini P, Varsani A.
Arch Virol. 2021 Oct;166(10):2937-2942. doi: 10.1007/s00705-021-05194-9

Active Case Finding of Current Bornavirus Infections in Human Encephalitis Cases of Unknown Etiology, Germany, 2018-2020
Eisermann P, Rubbenstroth D, Cadar D, Thomé-Bolduan C, Eggert P, Schlaphof A, Leypoldt F, Stangel M, Fortwängler T, Hoffmann F, Osterman A, Zange S, Niller HH, Angstwurm K, Pörtner K, Frank C, Wilking H, Beer M, Schmidt-Chanasit J, Tappe D
Emerg Infect Dis. 2021 May;27(5):1371-1379. doi: 10.3201/eid2705.204490

Introduction and spread of variegated squirrel bornavirus 1 (VSBV-1) between exotic squirrels and spill-over infections to humans in Germany
Cadar D, Allendorf V, Schulze V, Ulrich RG, Schlottau K, Ebinger A, Hoffmann B, Hoffmann D, Rubbenstroth D, Ismer G, Kibbey C, Marthaler A, Rissland J, Leypoldt F, Stangel M, Schmidt-Chanasit J, Conraths FJ, Beer M, Homeier-Bachmann T, Tappe D
Emerg Microbes Infect. 2021 Dec;10(1):602-611. doi: 10.1080/22221751.2021.1902752

Human Borna disease virus 1 (BoDV-1) encephalitis cases in the north and east of Germany
Christina Frank, Jonathan Wickel, Dirk Brämer, Jakob Matschke, Richard Ibe, Caroline Gazivoda, Albrecht Günther, Christian Hartmann, Kordt Rehn, Daniel Cadar, Thomas E Mayer, Kirsten Pörtner, Hendrik Wilking, Jonas Schmidt-Chanasit, Dennis Tappe
Emerg Microbes Infect . 2021 Nov 16;1-19. doi: 10.1080/22221751.2021.2007737

Vector Competence of the Invasive Mosquito Species Aedes koreicus for Arboviruses and Interference with a Novel Insect Specific Virus
Jansen S, Cadar D, Lühken R, Pfitzner WP, Jöst H, Oerther S, Helms M, Zibrat B, Kliemke K, Becker N, Vapalahti O, Rossini G, Heitmann A.
Viruses. 2021 Dec 14;13(12):2507. doi: 10.3390/v13122507.

Toscana virus encephalitis in Southwest Germany: a retrospective study
R Dersch, A Sophocleous, D Cadar, P Emmerich, J Schmidt-Chanasit, S Rauer
BMC Neurol . 2021 Dec 22;21(1):495. doi: 10.1186/s12883-021-02528-7.

2020

Severe Multiorgan Failure Following Yellow Fever Vaccination.
Domingo C, Lamerz J, Cadar D, Stojkovic M, Eisermann P, Merle U, Nitsche A, Schnitzler P
Vaccines (Basel). 2020 May 26;8(2):249. doi: 10.3390/vaccines8020249.

West Nile Virus Epidemic in Germany Triggered by Epizootic Emergence, 2019.
Ziegler U, Santos PD, Groschup MH, Hattendorf C, Eiden M, Höper D, Eisermann P, Keller M, Michel F, Klopfleisch R, Müller K, Werner D, Kampen H, Beer M, Frank C, Lachmann R, Tews BA, Wylezich C, Rinder M, Lachmann L, Grünewald T, Szentiks CA, Sieg M, Schmidt-Chanasit J, Cadar D, Lühken R
Viruses. 2020 Apr 15;12(4):448. doi: 10.3390/v12040448

On detection thresholds-a review on diagnostic approaches in the infectious disease laboratory and the interpretation of their results
Hahn A, Podbielski A, Meyer T, Zautner AE, Loderstädt U, Schwarz NG, Krüger A, Cadar D, Frickmann H
Acta Trop. 2020 May;205:105377. doi: 10.1016/j.actatropica.2020.105377

Comparative Metagenomics of Palearctic and Neotropical Avian Cloacal Viromes Reveal Geographic Bias in Virus Discovery
Truchado DA, Llanos-Garrido A, Oropesa-Olmedo DA, Cerrada B, Cea P, Moens MAJ, Gomez-Lucia E, Doménech A, Milá B, Pérez-Tris J, Cadar D, Benítez L.
Microorganisms. 2020 Nov 26;8(12):1869. doi: 10.3390/microorganisms8121869

Lassa fever in Benin: description of the 2014 and 2016 epidemics and genetic characterization of a new Lassa virus
Yadouleton A, Picard C, Rieger T, Loko F, Cadar D, Kouthon EC, Job EO, Bankolé H, Oestereich L, Gbaguidi F, Pahlman M, Becker-Ziaja B, Journeaux A, Pannetier D, Mély S, Mundweiler S, Thomas D, Kohossi L, Saizonou R, Kakaï CG, Da Silva M, Kossoubedie S, Kakonku AL, M'Pelé P, Gunther S, Baize S, Fichet-Calvet E.
Emerg Microbes Infect. 2020 Dec;9(1):1761-1770. doi: 10.1080/22221751.2020.1796528

Usutu Virus Epizootic in Belgium in 2017 and 2018: Evidence of Virus Endemization and Ongoing Introduction Events
Benzarti E, Sarlet M, Franssen M, Cadar D, Schmidt-Chanasit J, Rivas JF, Linden A, Desmecht D, Garigliany M.
Vector Borne Zoonotic Dis. 2020 Jan;20(1):43-50. doi: 10.1089/vbz.2019.2469

Blood-meal analysis of Culicoides (Diptera: Ceratopogonidae) reveals a broad host range and new species records for Romania
Tomazatos A, Jöst H, Schulze J, Spînu M, Schmidt-Chanasit J, Cadar D, Lühken R
Parasit Vectors. 2020 Feb 17;13(1):79. doi: 10.1186/s13071-020-3938-1

Letea Virus: Comparative Genomics and Phylogenetic Analysis of a Novel Reassortant Orbivirus Discovered in Grass Snakes ( Natrix natrix)
Tomazatos A, Marschang RE, Maranda I, Baum H, Bialonski A, Spînu M, Lühken R, Schmidt-Chanasit J, Cadar D.
Viruses. 2020 Feb 21;12(2):243. doi: 10.3390/v12020243

2019

Phylogeography of Lassa Virus in Nigeria
Ehichioya DU, Dellicour S, Pahlmann M, Rieger T, Oestereich L, Becker-Ziaja B, Cadar D, Ighodalo Y, Olokor T, Omomoh E, Oyakhilome J, Omiunu R, Agbukor J, Ebo B, Aiyepada J, Ebhodaghe P, Osiemi B, Ehikhametalor S, Akhilomen P, Airende M, Esumeh R, Muoebonam E, Giwa R, Ekanem A, Igenegbale G, Odigie G, Okonofua G, Enigbe R, Omonegho Yerumoh E, Pallasch E, Bockholt S, Kafetzopoulou LE, Duraffour S, Okokhere PO, Akpede GO, Okogbenin SA, Odia I, Aire C, Akpede N, Tobin E, Ogbaini-Emovon E, Lemey P, Adomeh DI, Asogun DA, Günther S.
Phylogeography of Lassa Virus in Nigeria. Ehichioya DU, Dellicour S, Pahlmann M, Rieger T, Oestereich L, Becker-Ziaja B, Cadar D, Ighodalo Y, Olokor T, Omomoh E, Oyakhilome J, Omiunu R, Agbukor J, Ebo B, Aiyepada J, Ebhodaghe P, Osiemi B, Ehikhametalor S, Akhilomen P, Airende M, Esumeh R, Muoebonam E, Giwa R, Ekanem A, Igenegbale G, Odigie G, Okonofua G, Enigbe R, Omonegho Yerumoh E, Pallasch E, Bockholt S, Kafetzopoulou LE, Duraffour S, Okokhere PO, Akpede GO, Okogbenin SA, Odia I, Aire C, Akpede N, Tobin E, Ogbaini-Emovon E, Lemey P, Adomeh DI, Asogun DA, Günther S. J Virol. 2019 Oct 15;93(21):e00929-19. doi: 10.1128/JVI.00929-19.

West Nile virus epizootic in Germany, 2018.
Ziegler U, Lühken R, Keller M, Cadar D, van der Grinten E, Michel F, Albrecht K, Eiden M, Rinder M, Lachmann L, Höper D, Vina-Rodriguez A, Gaede W, Pohl A, Schmidt-Chanasit J, Groschup MH.
Antiviral Res. 2019 Feb;162:39-43. doi: 10.1016/j.antiviral.2018.12.005

Usutu Virus Epizootic in Belgium in 2017 and 2018: Evidence of Virus Endemization and Ongoing Introduction Events
Benzarti E, Sarlet M, Franssen M, Cadar D, Schmidt-Chanasit J, Rivas JF, Linden A, Desmecht D, Garigliany M.
Vector Borne Zoonotic Dis. 2020 Jan;20(1):43-50. doi: 10.1089/vbz.2019.2469

Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins
Rockstroh A, Barzon L, Kumbukgolla W, Su HX, Lizarazo E, Vincenti-Gonzalez MF, Tami A, Ornelas AMM, Aguiar RS, Cadar D, Schmidt-Chanasit J, Ulbert S
Emerg Infect Dis. 2019 Jan;25(1):1111-1115. doi: 10.3201/eid2501.180605

Viral metagenomics revealed novel betatorquevirus species in pediatric inpatients with encephalitis/meningoencephalitis from Ghana
Eibach D, Hogan B, Sarpong N, Winter D, Struck NS, Adu-Sarkodie Y, Owusu-Dabo E, Schmidt-Chanasit J, May J, Cadar D.
Sci Rep. 2019 Feb 20;9(1):2360. doi: 10.1038/s41598-019-38975-z

Ecology of West Nile Virus in the Danube Delta, Romania: Phylogeography, Xenosurveillance and Mosquito Host-Feeding Patterns
Tomazatos A, Jansen S, Pfister S, Török E, Maranda I, Horváth C, Keresztes L, Spînu M, Tannich E, Jöst H, Schmidt-Chanasit J, Cadar D, Lühken R.
Viruses. 2019 Dec 14;11(12):1159. doi: 10.3390/v11121159

A novel group of avian astroviruses from Neotropical passerine birds broaden the diversity and host range of Astroviridae
Fernández-Correa I, Truchado DA, Gomez-Lucia E, Doménech A, Pérez-Tris J, Schmidt-Chanasit J, Cadar D, Benítez L.
Sci Rep. 2019 Jul 2;9(1):9513. doi: 10.1038/s41598-019-45889-3

A Novel and Divergent Gyrovirus with Unusual Genomic Features Detected in Wild Passerine Birds from a Remote Rainforest in French Guiana
Truchado DA, Diaz-Piqueras JM, Gomez-Lucia E, Doménech A, Milá B, Pérez-Tris J, Schmidt-Chanasit J, Cadar D, Benítez L.
Viruses. 2019 Dec 11;11(12):1148. doi: 10.3390/v11121148

2018

New Lineage of Lassa Virus, Togo, 2016
Whitmer SLM, Strecker T, Cadar D, Dienes HP, Faber K, Patel K, Brown SM, Davis WG, Klena JD, Rollin PE, Schmidt-Chanasit J, Fichet-Calvet E, Noack B, Emmerich P, Rieger T, Wolff S, Fehling SK, Eickmann M, Mengel JP, Schultze T, Hain T, Ampofo W, Bonney K, Aryeequaye JND, Ribner B, Varkey JB, Mehta AK, Lyon GM 3rd, Kann G, De Leuw P, Schuettfort G, Stephan C, Wieland U, Fries JWU, Kochanek M, Kraft CS, Wolf T, Nichol ST, Becker S, Ströher U, Günther S
Emerg Infect Dis. 2018 Mar;24(3):599-602. doi: 10.3201/eid2403.171905

Viral metagenomics, genetic and evolutionary characteristics of Crimean-Congo hemorrhagic fever orthonairovirus in humans, Kosovo
Emmerich P, Jakupi X, von Possel R, Berisha L, Halili B, Günther S, Cadar D, Ahmeti S, Schmidt-Chanasit J
Infect Genet Evol. 2018 Nov;65:6-11. doi: 10.1016/j.meegid.2018.07.010

Occupation-Associated Fatal Limbic Encephalitis Caused by Variegated Squirrel Bornavirus 1, Germany, 2013
Tappe D, Schlottau K, Cadar D, Hoffmann B, Balke L, Bewig B, Hoffmann D, Eisermann P, Fickenscher H, Krumbholz A, Laufs H, Huhndorf M, Rosenthal M, Schulz-Schaeffer W, Ismer G, Hotop SK, Brönstrup M, Ott A, Schmidt-Chanasit J, Beer M.
Emerg Infect Dis. 2018 Jun;24(6):978-987. doi: 10.3201/eid2406.172027

Investigating porcine parvoviruses genogroup 2 infection using in situ polymerase chain reaction
Novosel D, Cadar D, Tuboly T, Jungic A, Stadejek T, Ait-Ali T, Cságola A.
BMC Vet Res. 2018 May 21;14(1):163. doi: 10.1186/s12917-018-1487-z.

Circulation of Dirofilaria immitis and Dirofilaria repens in the Danube Delta Biosphere Reserve, Romania
Tomazatos A, Cadar D, Török E, Maranda I, Horváth C, Keresztes L, Spinu M, Jansen S, Jöst H, Schmidt-Chanasit J, Tannich E, Lühken R.
Parasit Vectors. 2018 Jul 4;11(1):392. doi: 10.1186/s13071-018-2980-8

Male offspring born to mildly ZIKV-infected mice are at risk of developing neurocognitive disorders in adulthood
Stanelle-Bertram S, Walendy-Gnirß K, Speiseder T, Thiele S, Asante IA, Dreier C, Kouassi NM, Preuß A, Pilnitz-Stolze G, Müller U, Thanisch S, Richter M, Scharrenberg R, Kraus V, Dörk R, Schau L, Herder V, Gerhauser I, Pfankuche VM, Käufer C, Waltl I, Moraes T, Sellau J, Hoenow S, Schmidt-Chanasit J, Jansen S, Schattling B, Ittrich H, Bartsch U, Renné T, Bartenschlager R, Arck P, Cadar D, Friese MA, Vapalahti O, Lotter H, Benites S, Rolling L, Gabriel M, Baumgärtner W, Morellini F, Hölter SM, Amarie O, Fuchs H, Hrabe de Angelis M, Löscher W, Calderon de Anda F, Gabriel G.
Nat Microbiol. 2018 Oct;3(10):1161-1174. doi: 10.1038/s41564-018-0236-1.

Malaria Coinfections in Febrile Pediatric Inpatients: A Hospital-Based Study From Ghana
Hogan B, Eibach D, Krumkamp R, Sarpong N, Dekker D, Kreuels B, Maiga-Ascofaré O, Gyau Boahen K, Wiafe Akenten C, Adu-Sarkodie Y, Owusu-Dabo E, May J; Fever Without Source (FWS) Study Group.
Clin Infect Dis. 2018 Jun 1;66(12):1838-1845. doi: 10.1093/cid/cix1120

Genetic diversity of PRRSV 1 in Central Eastern Europe in 1994-2014: origin and evolution of the virus in the region
Balka G, Podgórska K, Brar MS, Bálint Á, Cadar D, Celer V, Dénes L, Dirbakova Z, Jedryczko A, Márton L, Novosel D, Petrović T, Sirakov I, Szalay D, Toplak I, Leung FC, Stadejek T
Sci Rep. 2018 May 17;8(1):7811. doi: 10.1038/s41598-018-26036-w

2017

Early Evidence for Zika Virus Circulation among Aedes aegypti Mosquitoes, Rio de Janeiro, Brazil
Ayllón T, Campos RM, Brasil P, Morone FC, Câmara DCP, Meira GLS, Tannich E, Yamamoto KA, Carvalho MS, Pedro RS, Schmidt-Chanasit J, Cadar D, Ferreira DF, Honório NA.
Emerg Infect Dis. 2017 Aug;23(8):1411-1412. doi: 10.3201/eid2308.162007

Moku Virus in Invasive Asian Hornets, Belgium, 2016.
Garigliany M, Taminiau B, El Agrebi N, Cadar D, Gilliaux G, Hue M, Desmecht D, Daube G, Linden A, Farnir F, De Proft M, Saegerman C.
Emerg Infect Dis. 2017 Dec;23(12):2109-2112. doi: 10.3201/eid2312.171080

Distribution of Usutu Virus in Germany and Its Effect on Breeding Bird Populations
Lühken R, Jöst H, Cadar D, Thomas SM, Bosch S, Tannich E, Becker N, Ziegler U, Lachmann L, Schmidt-Chanasit J
Emerg Infect Dis. 2017 Dec;23(12):1994-2001. doi: 10.3201/eid2312.171257

Widespread activity of multiple lineages of Usutu virus, western Europe, 2016
Cadar D, Lühken R, van der Jeugd H, Garigliany M, Ziegler U, Keller M, Lahoreau J, Lachmann L, Becker N, Kik M, Oude Munnink BB, Bosch S, Tannich E, Linden A, Schmidt V, Koopmans MP, Rijks J, Desmecht D, Groschup MH, Reusken C, Schmidt-Chanasit J.
Euro Surveill. 2017 Jan 26;22(4):30452. doi: 10.2807/1560-7917.ES.2017.22.4.30452

Genetic Diversity and New Lineages of Dengue Virus Serotypes 3 and 4 in Returning Travelers, Germany, 2006-2015
Shihada S, Emmerich P, Thomé-Bolduan C, Jansen S, Günther S, Frank C, Schmidt-Chanasit J, Cadar D.
Emerg Infect Dis. 2017 Feb;23(2):272-275. doi: 10.3201/eid2302.160751.

Control measures following a case of imported Lassa fever from Togo, North Rhine Westphalia, Germany, 2016
Lehmann C, Kochanek M, Abdulla D, Becker S, Böll B, Bunte A, Cadar D, Dormann A, Eickmann M, Emmerich P, Feldt T, Frank C, Fries J, Gabriel M, Goetsch U, Gottschalk R, Günther S, Hallek M, Häussinger D, Herzog C, Jensen B, Kolibay F, Krakau M, Langebartels G, Rieger T, Schaade L, Schmidt-Chanasit J, Schömig E, Schüttfort G, Shimabukuro-Vornhagen A, von Bergwelt-Baildon M, Wieland U, Wiesmüller G, Wolf T, Fätkenheuer G.
Euro Surveill. 2017 Sep;22(39):17-00088. doi: 10.2807/1560-7917.ES.2017.22.39.17-00088

Detection and characterization of a novel rhabdovirus in Aedes cantans mosquitoes and evidence for a mosquito-associated new genus in the family Rhabdoviridae
Shahhosseini N, Lühken R, Jöst H, Jansen S, Börstler J, Rieger T, Krüger A, Yadouleton A, de Mendonça Campos R, Cirne-Santos CC, Ferreira DF, Garms R, Becker N, Tannich E, Cadar D, Schmidt-Chanasit J.
Infect Genet Evol. 2017 Nov;55:260-268. doi: 10.1016/j.meegid.2017.09.026.

Blood donor screening for West Nile virus (WNV) revealed acute Usutu virus (USUV) infection, Germany, September 2016
Cadar D, Maier P, Müller S, Kress J, Chudy M, Bialonski A, Schlaphof A, Jansen S, Jöst H, Tannich E, Runkel S, Hitzler WE, Hutschenreuter G, Wessiepe M, Schmidt-Chanasit J.
Euro Surveill. 2017 Apr 6;22(14):30501. doi: 10.2807/1560-7917.ES.2017.22.14.30501

2016

New Hosts of The Lassa Virus
Olayemi A, Cadar D, Magassouba N, Obadare A, Kourouma F, Oyeyiola A, Fasogbon S, Igbokwe J, Rieger T, Bockholt S, Jérôme H, Schmidt-Chanasit J, Garigliany M, Lorenzen S, Igbahenah F, Fichet JN, Ortsega D, Omilabu S, Günther S, Fichet-Calvet E.
Sci Rep. 2016 May 3;6:25280. doi: 10.1038/srep25280

Assay optimization for molecular detection of Zika virus
Corman VM, Rasche A, Baronti C, Aldabbagh S, Cadar D, Reusken CB, Pas SD, Goorhuis A, Schinkel J, Molenkamp R, Kümmerer BM, Bleicker T, Brünink S, Eschbach-Bludau M, Eis-Hübinger AM, Koopmans MP, Schmidt-Chanasit J, Grobusch MP, de Lamballerie X, Drosten C, Drexler JF.
Bull World Health Organ. 2016 Dec 1;94(12):880-892. doi: 10.2471/BLT.16.175950

Sexual transmission of Zika virus in Germany, April 2016
Frank C, Cadar D, Schlaphof A, Neddersen N, Günther S, Schmidt-Chanasit J, Tappe D.
Euro Surveill. 2016 Jun 9;21(23). doi: 10.2807/1560-7917.ES.2016.21.23.30252

Evidence for an independent third Usutu virus introduction into Germany
Ziegler U, Fast C, Eiden M, Bock S, Schulze C, Hoeper D, Ochs A, Schlieben P, Keller M, Zielke DE, Luehken R, Cadar D, Walther D, Schmidt-Chanasit J, Groschup MH
Vet Microbiol. 2016 Aug 30;192:60-66. doi: 10.1016/j.vetmic.2016.06.007.

Reconstruction of the Evolutionary History and Dispersal of Usutu Virus, a Neglected Emerging Arbovirus in Europe and Africa
Engel D, Jöst H, Wink M, Börstler J, Bosch S, Garigliany MM, Jöst A, Czajka C, Lühken R, Ziegler U, Groschup MH, Pfeffer M, Becker N, Cadar D, Schmidt-Chanasit J.
mBio. 2016 Feb 2;7(1):e01938-15. doi: 10.1128/mBio.01938-15

Pilot longitudinal mosquito surveillance study in the Danube Delta Biosphere Reserve and the first reports of Anopheles algeriensis Theobald, 1903 and Aedes hungaricus Mihályi, 1955 for Romania
Török E, Tomazatos A, Cadar D, Horváth C, Keresztes L, Jansen S, Becker N, Kaiser A, Popescu O, Schmidt-Chanasit J, Jöst H, Lühken R.
Parasit Vectors. 2016 Apr 11;9:196. doi: 10.1186/s13071-016-1484-7

2015

Surveillance of Batai virus in bovines from Germany
Hofmann M, Wiethölter A, Blaha I, Jöst H, Heinemann P, Lehmann M, Miller T, Cadar D, Yanase T, Kley N, Eiden M, Groschup M, Schmidt-Chanasit J.
Clin Vaccine Immunol. 2015 Jun;22(6):672-3. doi: 10.1128/CVI.00082-15

Putative Lineage of Novel African Usutu Virus, Central Europe
Hofmann M, Wiethölter A, Blaha I, Jöst H, Heinemann P, Lehmann M, Miller T, Cadar D, Yanase T, Kley N, Eiden M, Groschup M, Schmidt-Chanasit J.
Clin Vaccine Immunol. 2015 Jun;22(6):672-3. doi: 10.1128/CVI.00082-15

Emergence of multireassortant bluetongue virus serotype 4 in Hungary
Hornyák Á, Malik P, Marton S, Dóró R, Cadar D, Bányai K.
Emergence of multireassortant bluetongue virus serotype 4 in Hungary. Hornyák Á, Malik P, Marton S, Dóró R, Cadar D, Bányai K. Infect Genet Evol. 2015 Jul;33:6-10. doi: 10.1016/j.meegid.2015.03.036.

Co-circulation of a novel phlebovirus and Massilia virus in sandflies, Portugal
Amaro F, Zé-Zé L, Alves MJ, Börstler J, Clos J, Lorenzen S, Becker SC, Schmidt-Chanasit J, Cadar D.
Virol J. 2015 Oct 24;12:174. doi: 10.1186/s12985-015-0407-0.

A Variegated Squirrel Bornavirus Associated with Fatal Human Encephalitis
Hoffmann B, Tappe D, Höper D, Herden C, Boldt A, Mawrin C, Niederstraßer O, Müller T, Jenckel M, van der Grinten E, Lutter C, Abendroth B, Teifke JP, Cadar D, Schmidt-Chanasit J, Ulrich RG, Beer M
N Engl J Med. 2015 Jul 9;373(2):154-62. doi: 10.1056/NEJMoa1415627

Mammalophilic feeding behaviour of Culex quinquefasciatus mosquitoes collected in the cities of Chetumal and Cancun, Yucatán Peninsula, Mexico
Janssen N, Fernandez-Salas I, Díaz González EE, Gaytan-Burns A, Medina-de la Garza CE, Sanchez-Casas RM, Börstler J, Cadar D, Schmidt-Chanasit J, Jöst H.
Trop Med Int Health. 2015 Nov;20(11):1488-1491. doi: 10.1111/tmi.12587

Characterization of a novel circo-like virus in Aedes vexans mosquitoes from Germany: evidence for a new genus within the family Circoviridae
Garigliany MM, Börstler J, Jöst H, Badusche M, Desmecht D, Schmidt-Chanasit J, Cadar D
J Gen Virol. 2015 Apr;96(Pt 4):915-920. doi: 10.1099/vir.0.000036.

Zika virus infections imported to Italy: clinical, immunological and virological findings, and public health implications
Zammarchi L, Stella G, Mantella A, Bartolozzi D, Tappe D, Günther S, Oestereich L, Cadar D, Muñoz-Fontela C, Bartoloni A, Schmidt-Chanasit J.
J Clin Virol. 2015 Feb;63:32-5. doi: 10.1016/j.jcv.2014.12.005.

2014

Usutu virus in bats, Germany, 2013
Cadar D, Becker N, Campos Rde M, Börstler J, Jöst H, Schmidt-Chanasit J.
Emerg Infect Dis. 2014 Oct;20(10):1771-3. doi: 10.3201/eid2010.140909.

Human Dobrava-Belgrade hantavirus infection, Kosovo
Emmerich P, Müller N, Heinemann P, Rother E, Jakupi X, Günther S, Cadar D, Schmidt-Chanasit
J Clin Virol. 2014 Nov;61(3):439-41. doi: 10.1016/j.jcv.2014.09.013

Emergence of Zaire Ebola virus disease in Guinea
Baize S, Pannetier D, Oestereich L, Rieger T, Koivogui L, Magassouba N, Soropogui B, Sow MS, Keïta S, De Clerck H, Tiffany A, Dominguez G, Loua M, Traoré A, Kolié M, Malano ER, Heleze E, Bocquin A, Mély S, Raoul H, Caro V, Cadar D, Gabriel M, Pahlmann M, Tappe D, Schmidt-Chanasit J, Impouma B, Diallo AK, Formenty P, Van Herp M, Günther S.
N Engl J Med. 2014 Oct 9;371(15):1418-25. doi: 10.1056/NEJMoa1404505

Cyclovirus CyCV-VN species distribution is not limited to Vietnam and extends to Africa
Garigliany MM, Hagen RM, Frickmann H, May J, Schwarz NG, Perse A, Jöst H, Börstler J, Shahhosseini N, Desmecht D, Mbunkah HA, Daniel AM, Kingsley MT, Campos Rde M, de Paula VS, Randriamampionona N, Poppert S, Tannich E, Rakotozandrindrainy R, Cadar D, Schmidt-Chanasit J.
Sci Rep. 2014 Dec 18;4:7552. doi: 10.1038/srep07552.

Zika virus infection after travel to Tahiti, December 2013
Wæhre T, Maagard A, Tappe D, Cadar D, Schmidt-Chanasit J.
Emerg Infect Dis. 2014 Aug;20(8):1412-4. doi: 10.3201/eid2008.140302

Detection of Puumala hantavirus antigen in human intestine during acute hantavirus infection
Latus J, Tenner-Racz K, Racz P, Kitterer D, Cadar D, Ott G, Alscher MD, Schmidt-Chanasit J, Braun N
PLoS One. 2014 May 23;9(5):e98397. doi: 10.1371/journal.pone.0098397.

O'nyong-nyong virus infection imported to Europe from Kenya by a traveler.
Tappe D, Kapaun A, Emmerich P, Campos Rde M, Cadar D, Günther S, Schmidt-Chanasit J
Emerg Infect Dis. 2014 Oct;20(10):1766-7. doi: 10.3201/eid2010.140823.

Genome analysis of Mayaro virus imported to Germany from French Guiana
Friedrich-Jänicke B, Emmerich P, Tappe D, Günther S, Cadar D, Schmidt-Chanasit J.
Emerg Infect Dis. 2014 Jul;20(7):1255-7. doi: 10.3201/eid2007.140043.

Crimean-Congo hemorrhagic fever virus clades V and VI (Europe 1 and 2) in ticks in Kosovo, 2012
Sherifi K, Cadar D, Muji S, Robaj A, Ahmeti S, Jakupi X, Emmerich P, Krüger A.
PLoS Negl Trop Dis. 2014 Sep 25;8(9):e3168. doi: 10.1371/journal.pntd.0003168.

Characterization of a novel circo-like virus in Aedes vexans mosquitoes from Germany: evidence for a new genus within the family Circoviridae
Garigliany MM, Börstler J, Jöst H, Badusche M, Desmecht D, Schmidt-Chanasit J, Cadar D
J Gen Virol. 2015 Apr;96(Pt 4):915-920. doi: 10.1099/vir.0.000036.

Virus Discovery, Ecology and Evolution

NGS Core Facility

Contact

Dániel Cadar, DVM, MPH, PhD

Phone: +49 40 42818-840
Fax: +49 40 42818-400
E-Mail: cadar@bnitm.de


Scientific Staff

Balázs Horváth , PhD (-840)
Alex Tomazatos, PhD (-840)

Associated Scientist

Gabriel da Luz Wallau, PhD -840

Technical Staff

Alexandra Bialonski (-835)
Heike Baum (-835)
Marike Petersen (-835)

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