Our publications
2023
The Kelch13 compartment contains highly divergent vesicle trafficking proteins in malaria parasites
- Schmidt S*, Wichers-Misterek JS*, Behrens HM, Birnbaum J, Henshall IC, Dröge J, Jonscher E, Flemming S, Castro-Peña C, Mesén-Ramírez P, Spielmann T (* equal first authors)
- Plos Pathogens, in press
Gene-by-gene screen of the unknown proteins encoded on Plasmodium falciparum chromosome 3
- Kimmel J, Schmitt M, Sinner A, Jansen PWTC, Mainye S, Ramón-Zamorano G, Toenhake CG, Wichers-Misterek JS, Cronshagen J, Sabitzki R, Mesén-Ramírez P, Behrens HM, Bártfai R, Spielmann T
- Cell Syst. 2023 Jan 18;14(1):9-23.e7. doi: 10.1016/j.cels.2022.12.001
Clearing of hemozoin crystals in malaria parasites enables whole-cell STED microscopy
- Kehrer J, Pietsch E, Heinze J, Spielmann T, Frischknecht F
- J Cell Sci. 2023 Jan 1;136(1):jcs260399. doi: 10.1242/jcs.260399
2022
PMRT1, a Plasmodium-Specific Parasite Plasma Membrane Transporter, Is Essential for Asexual and Sexual Blood Stage Development
- Wichers JS, Mesén-Ramírez P, Fuchs G, Yu-Strzelczyk J, Stäcker J, von Thien H, Alder A, Henshall I, Liffner B, Nagel G, Löw C, Wilson D, Spielmann T, Gao S, Gilberger TW, Bachmann A, Strauss J
- mBio. 2022 Apr 26;13(2):e0062322. doi: 10.1128/mbio.00623-22
Proximity-dependent biotinylation approaches to study apicomplexan biology
- Kimmel J, Kehrer J, Frischknecht F, Spielmann T
- Mol Microbiol. 2022 Mar;117(3):553-568. doi: 10.1111/mmi.14815
2021
Characterization of Apicomplexan Amino Acid Transporters (ApiATs) in the Malaria Parasite Plasmodium falciparum
- Wichers JS, van Gelder C, Fuchs G, Ruge JM, Pietsch E, Ferreira JL, Safavi S, von Thien H, Burda PC, Mesén-Ramirez P, Spielmann T, Strauss J, Gilberger TW, Bachmann A
- mSphere. 2021 Dec 22;6(6):e0074321. doi: 10.1128/mSphere.00743-21. Epub 2021 Nov 10
The parasitophorous vacuole nutrient channel is critical for drug access in malaria parasites and modulates the artemisinin resistance fitness cost
- Mesén-Ramírez P, Bergmann B, Elhabiri M, Zhu L, von Thien H, Castro-Peña C, Gilberger TW, Davioud-Charvet E, Bozdech Z, Bachmann A, Spielmann T
- Cell Host Microbe. 2021 Dec 8;29(12):1774-1787.e9. doi: 10.1016/j.chom.2021.11.002. Epub 2021 Dec 3. Comment in Compensating P. falciparum artemisinin resistance. Platon L, Cao J, Ménard D. Cell Host Microbe. 2021 Dec 8;29(12):1732-1734. doi: 10.1016
The newly discovered role of endocytosis in artemisinin resistance
- Behrens HM, Schmidt S, Spielmann T
- Med Res Rev. 2021 Nov;41(6):2998-3022. doi: 10.1002/med.21848
Identification of novel inner membrane complex and apical annuli proteins of the malaria parasite Plasmodium falciparum
- Wichers JS, Wunderlich J, Heincke D, Pazicky S, Strauss J, Schmitt M, Kimmel J, Wilcke L, Scharf S, von Thien H, Burda PC, Spielmann T, Löw C, Filarsky M, Bachmann A, Gilberger TW
- Cell Microbiol. 2021 Sep;23(9):e13341. doi: 10.1111/cmi.13341
2020
The ZIP Code of Vesicle Trafficking in Apicomplexa: SEC1/Munc18 and SNARE Proteins
- Bisio H, Chaabene RB, Sabitzki R, Maco B, Marq JB, Gilberger TW, Spielmann T, Soldati-Favre D
- mBio. 2020 Oct 20;11(5):e02092-20. doi: 10.1128/mBio.02092-20
Introduction of Scaffold Nitrogen Atoms Renders Inhibitors of the Malarial l-Lactate Transporter, PfFNT, Effective against the Gly107Ser Resistance Mutation
- Walloch P, Henke B, Häuer S, Bergmann B, Spielmann T, Beitz E
- J Med Chem. 2020 Sep 10;63(17):9731-9741. doi: 10.1021/acs.jmedchem.0c00852
Endocytosis in Plasmodium and Toxoplasma Parasites
- Spielmann T, Gras S, Sabitzki R, Meissner M
- Trends Parasitol.2020 Jun;36(6):520-532. doi: 10.1016/j.pt.2020.03.010
A Kelch13-defined endocytosis pathway mediates artemisinin resistance in malaria parasites
- Birnbaum J*, Scharf S*, Schmidt S, Jonscher E, Hoeijmakers WAM, Flemming S, Toenhake CG, Schmitt M, Sabitzki R, Bergmann B, Fröhlke U, Mesen-Ramirez P, Blancke Soares A, Herrmann H, Bártfai R and Spielmann T
- Science. 2020 Jan 3;367(6473):51-59. doi: 10.1126/science.aax4735
2019
Epigenetic reader complexes of the human malaria parasite, Plasmodium falciparum
- Hoeijmakers WAM, Miao J, Schmidt S, Toenhake CG, Shrestha S, Venhuizen J, Henderson R, Birnbaum J, Ghidelli-Disse S, Drewes G, Cui L, Stunnenberg HG, Spielmann T, Bártfai R
- Nucleic Acids Res. 2019 Dec 16;47(22):11574-11588. doi: 10.1093/nar/gkz1044
EXP1 is critical for nutrient uptake across the parasitophorous vacuole membrane of malaria parasites
- Mesén-Ramírez P, Bergmann B, Tran TT, Garten M, Stäcker J, Naranjo-Prado I, Höhn K, Zimmerberg J, Spielmann T
- PLoS Biol. 2019 Sep 30;17(9):e3000473. doi: 10.1371/journal.pbio.3000473
Delayed death in the malaria parasite Plasmodium falciparum is caused by disruption of prenylation-dependent intracellular trafficking
- Kennedy K, Cobbold SA, Hanssen E, Birnbaum J, Spillman NJ, McHugh E, Brown H, Tilley L, Spielmann T, McConville MJ, Ralph SA
- PLoS Biol. 2019 Jul 18;17(7):e3000376. doi: 10.1371/journal.pbio.3000376
PfVPS45 Is Required for Host Cell Cytosol Uptake by Malaria Blood Stage Parasites
- Jonscher E, Flemming S, Schmitt M, Sabitzki R, Reichard N, Birnbaum J, Bergmann B, Höhn K, Spielmann T
- Cell Host Microbe. 2019 Jan 9;25(1):166-173.e5. doi: 10.1016/j.chom.2018.11.010
2018
Identification of novel parasitophorous vacuole proteins in P. falciparum parasites using BioID
- Khosh-Naucke M, Becker J, Mesén-Ramírez P, Kiani P, Birnbaum J, Fröhlke U, Jonscher E, Schlüter H, Spielmann T
- Int J Med Microbiol. 2018 Jan;308(1):13-24. doi: 10.1016/j.ijmm.2017.07.007
2017
A genetic system to study Plasmodium falciparum protein function
- Birnbaum J, Flemming S, Reichard N, Soares AB, Mesén-Ramírez P, Jonscher E, Bergmann B, Spielmann T
- Nat Methods. 2017 Apr;14(4):450-456. doi: 10.1038/nmeth.422
Substrate-analogous inhibitors exert antimalarial action by targeting the Plasmodium lactate transporter PfFNT at nanomolar scale
- Golldack A, Henke B, Bergmann B, Wiechert M, Erler H, Blancke Soares A, Spielmann T, Beitz E
- PLoS Pathog. 2017 Feb 8;13(2):e1006172. doi: 10.1371/journal.ppat.1006172
Progress in imaging methods: insights gained into Plasmodium biology
- De Niz M, Burda PC, Kaiser G, Del Portillo HA, Spielmann T, Frischknecht F, Heussler VT
- Nat Rev Microbiol. 2017 Jan;15(1):37-54. doi: 10.1038/nrmicro.2016.158
2016
Stable Translocation Intermediates Jam Global Protein Export in Plasmodium falciparum Parasites and Link the PTEX Component EXP2 with Translocation Activity
- Mesén-Ramírez P, Reinsch F, Blancke Soares A, Bergmann B, Ullrich AK, Tenzer S, Spielmann T
- PLoS Pathog. 2016;12(5):e1005618. doi: 10.1371/journal.ppat.1005618
Hierarchical phosphorylation of apical membrane antigen 1 is required for efficient red blood cell invasion by malaria parasites
- Prinz B, Harvey KL, Wilcke L, Ruch U, Engelberg K, Biller L, Lucet I, Erkelenz S, Heincke D, Spielmann T, Doerig C, Kunick C, Crabb BS, Gilson PR, Gilberger TW
- Sci Rep. 2016 Oct 4;6:34479. doi: 10.1038/srep34479
Export of malaria proteins requires co-translational processing of the PEXEL motif independent of phosphatidylinositol-3-phosphate binding
- Boddey JA, O'Neill MT, Lopaticki S, Carvalho TG, Hodder AN, Nebl T, Wawra S, van West P, Ebrahimzadeh Z, Richard D, Flemming S, Spielmann T, Przyborski J, Babon JJ, Cowman AF
- Nat Commun. 2016 Feb 1;7:10470. doi: 10.1038/ncomms10470
The machinery underlying malaria parasite virulence is conserved between rodent and human malaria parasites
- De Niz M, Ullrich AK, Heiber A, Blancke Soares A, Pick C, Lyck R, Keller D, Kaiser G, Prado M, Flemming S, Del Portillo H, Janse CJ, Heussler V, Spielmann T
- Nat Commun. 2016;7:11659. doi: 10.1038/ncomms11659
Pellicle formation in the malaria parasite
- Kono M, Heincke D, Wilcke L, Wong T, Bruns C, Herrmann S, Spielmann T, Gilberger TW
- J Cell Sci. 2016, 129(4):673-80. doi: 10.1242/jcs.181230
2015
Critical Steps in Protein Export of Plasmodium falciparum Blood Stages
- Spielmann T, Gilberger TW
- Trends Parasitol. 2015, 31(10):514-25. doi: 10.1016/j.pt.2015.06.010
Identity of a Plasmodium lactate/H(+) symporter structurally unrelated to human transporters
- Wu, B., Rambow, J., Bock, S., Holm-Bertelsen, J., Wiechert, M., Soares, A.B., Spielmann, T., Beitz, E
- Nat. Commun. 2015, 6:6284. doi: 10.1038/ncomms7284
The Plasmodium falciparum exportome contains non-canonical PEXEL/HT proteins
- Schulze J, Kwiatkowski M, Borner J, Schlüter H, Bruchhaus I, Burmester T, Spielmann T#, Pick C# (#, equal contributors)
- Mol Microbiol. 2015, 97:301-14. doi: 10.1111/mmi.13024
The role of palmitoylation for protein recruitment to the inner membrane complex of the malaria parasite
- Wetzel J, Herrmann S, Swapna LS, Prusty D, John Peter AT, Kono M, Saini S, Nellimarla S, Wong TW, Wilcke L, Ramsay O, Cabrera A, Biller L, Heincke D, Mossman K, Spielmann T, Ungermann C, Parkinson J, Gilberger TW
- J. Biol. Chem. 2015, 290:1712-28. doi: 10.1074/jbc.M114.598094
2013
Identification of New PNEPs Indicates a Substantial Non-PEXEL Exportome and Underpins Common Features in Plasmodium falciparum Protein Export
- Heiber, A., Kruse, F., Pick, C., Grüring, C., Flemming, S., Oberli, A., Schoeler, H., Retzlaff, S., Mesén-Ramírez, P., Hiss, J.A., Kadekoppala, M., Hecht, L., Holder, A.H., Gilberger, T.-W., Spielmann, T
- PLoS Pathog. 2013;9(8):e1003546. doi: 10.1371/journal.ppat.1003546
Protein export in malaria parasites: many membranes to cross
- Marti M, Spielmann T
- Curr Opin Microbiol. 2013 Aug;16(4):445-51. doi: 10.1016/j.mib.2013.04.010
Specific phosphorylation of the PfRh2b invasion ligand of Plasmodium falciparum
- Engelberg K, Paul AS, Prinz B, Kono M, Ching W, Heincke D, Dobner T, Spielmann T, Duraisingh MT, Gilberger TW
- Biochem J. 2013 Jun 15;452(3):457-66. doi: 10.1042/BJ20121694
2012
Wherever I may roam: Protein and membrane trafficking in P. falciparum-infected red blood cells
- Deponte M, Hoppe HC, Lee MC, Maier AG, Richard D, Rug M, Spielmann T, Przyborski JM
- Mol Biochem Parasitol. 2012 Dec;186(2):95-116. doi: 10.1016/j.molbiopara.2012.09.007
Dissection of minimal sequence requirements for rhoptry membrane targeting in the malaria parasite
- Cabrera A, Herrmann S, Warszta D, Santos JM, John Peter AT, Kono M, Debrouver S, Jacobs T, Spielmann T, Ungermann C, Soldati-Favre D, Gilberger TW
- Traffic. 2012 Oct;13(10):1335-50. doi: 10.1111/j.1600-0854.2012.01394.x
Molecular make-up of the Plasmodium parasitophorous vacuolar membrane
- Spielmann T, Montagna GN, Hecht L, Matuschewski K
- Int J Med Microbiol. 2012 Oct;302(4-5):179-86. doi: 10.1016/j.ijmm.2012.07.011
Evolution and Architecture of the Inner Membrane Complex in Asexual and Sexual Stages of the Malaria Parasite
- Kono M, Herrmann S, Loughran NB, Cabrera A, Engelberg K, Lehmann C, Sinha D, Prinz B, Ruch U, Heussler V, Spielmann T, Parkinson J, Gilberger TW
- Mol Biol Evol. 2012 Sep;29(9):2113-32. doi: 10.1093/molbev/mss081
Host cell deformability is linked to transmission in the human malaria parasite Plasmodium falciparum
- Aingaran M, Zhang R, Law SK, Peng Z, Undisz A, Meyer E, Diez-Silva M, Burke TA, Spielmann T, Lim CT, Suresh S, Dao M, Marti M
- Cell Microbiol. 2012 Jul;14(7):983-93. doi: 10.1111/j.1462-5822.2012.01786.x
Uncovering common principles in protein export of malaria parasites
- Grüring C, Heiber A, Kruse F, Flemming S, Franci G, Colombo SF, Fasana E, Schoeler H, Borgese N, Stunnenberg HG, Przyborski JM, Gilberger TW, Spielmann T
- Cell Host Microbe. 2012 Nov 15;12(5):717-29. doi: 10.1016/j.chom.2012.09.010
Imaging of live malaria blood stage parasites
- Grüring C, Spielmann T
- Methods Enzymol. 2012;506:81-92. doi: 10.1016/B978-0-12-391856-7.00029-9
2011
Phylogenomic analyses of malaria parasites and evolution of their exported proteins
- Pick C, Ebersberger I, Spielmann T, Bruchhaus I, Burmester T
- BMC Evol Biol. 2011 Jun 15;11:167. doi: 10.1186/1471-2148-11-167
Development and Host Cell Modifications of Plasmodium falciparum Blood Stages in 4D
- Grüring C, Heiber A, Kruse F, Ungefehr J, Gilberger TW, Spielmann T
- Nat Commun. 2011 Jan 25;2:165. doi: 10.1038/ncomms1169
2010
Transcriptional profiling of growth perturbations of the human malaria parasite Plasmodium falciparum
- Hu G, Cabrera A, Kono M, Mok S, Chaal BK, Haase S, Engelberg K, Cheemadan S, Spielmann T, Preiser PR, Gilberger TW, Bozdech Z
- Nat Biotechnol. 2010 Jan;28(1):91-8. doi: 10.1038/nbt.1597
Protein export in malaria parasites: do multiple export motifs add up to multiple export pathways?
- Spielmann T, Gilberger TW
- Trends Parasitol. 2010 Jan;26(1):6-10. doi: 10.1016/j.pt.2009.10.001
2009
A novel Plasmodium falciparum erythrocyte binding protein associated with the merozoite surface, PfDBLMSP
- Wickramarachchi T, Cabrera AL, Sinha D, Dhawan S, Chandran T, Devi YS, Kono M, Spielmann T, Gilberger TW, Chauhan VS, Mohmmed A
- Int J Parasitol. 2009 Jun;39(7):763-73. doi: 10.1016/j.ijpara.2008.12.004
Functional analysis of the leading malaria vaccine candidate AMA-1 reveals an essential role for the cytoplasmic domain in the invasion process
- Treeck M, Zacherl S, Herrmann S, Cabrera A, Kono M, Struck NS, Engelberg K, Haase S, Frischknecht F, Miura K, Spielmann T, Gilberger TW
- PLoS Pathog. 2009 Mar;5(3):e1000322. doi: 10.1371/journal.ppat.1000322
Sequence requirements for the export of the P. falciparum Maurer's clefts protein REX2
- Haase S, Herrmann S, Grüring C, Heiber A, Jansen PW, Langer C, Treeck M, Cabrera A, Bruns C, Struck NS, Kono M, Engelberg K, Ruch U, Stunnenberg HG, Gilberger TW, Spielmann T
- Mol Microbiol. 2009 Feb;71(4):1003-17. doi: 10.1111/j.1365-2958.2008.06582.x
2008
Reliable transfection of Plasmodium falciparum using non-commercial plasmid mini preparations
- Spielmann T, Dixon MW, Hernandez-Valladares M, Hannemann M, Trenholme KR, Gardiner DL
- Int J Parasitol. 2006 Oct;36(12):1245-8. doi: 10.1016/j.ijpara.2006.06.017
Targeting of the Ring Exported Protein 1 to the Maurer's clefts is mediated by a two phase process.
- Dixon, M.W.A., Hawthorne, P.L., Spielmann, T., Anderson, K.L., Trenholme, K.R., and Gardiner, D.L.
- Traffic. 2008 Aug;9(8):1316-26. doi: 10.1111/j.1600-0854.2008.00768.x. Epub 2008 May 19.
Targeted mutagenesis of the ring exported protein-1 of Plasmodium falciparum disrupts the architecture of Maurer's cleft organelles
- Hanssen E, Hawthorne P, Dixon MW, Trenholme KR, McMillan PJ, Spielmann T, Gardiner DL, Tilley L
- Mol Microbiol. 2008 Aug;69(4):938-53. doi: 10.1111/j.1365-2958.2008.06329.x
Plasmodium falciparum possesses two GRASP proteins that are differentially targeted to the golgi apparatus via a higher and lower eukaryote-like mechanism
- Struck NS, Herrmann S, Langer C, Krueger A, Foth BJ, Engelberg K, Cabrera AL, Haase S, Treeck M, Marti M, Cowman AF, Spielmann T, Gilberger TW
- J Cell Sci. 2008 Jul 1;121(Pt 13):2123-9. doi: 10.1242/jcs.021154
Spatial dissection of the cis- and trans-Golgi compartments in the malaria parasite Plasmodium falciparum
- Struck NS, Herrmann S, Schmuck-Barkmann I, de Souza Dias S, Haase S, Cabrera AL, Treeck M, Bruns C, Langer C, Cowman AF, Marti M, Spielmann T, Gilberger TW
- Mol Microbiol. 2008 Mar;67(6):1320-30. doi: 10.1111/j.1365-2958.2008.06125.x
Characterization of a conserved rhoptry-associated leucine zipper-like protein in the malaria parasite Plasmodium falciparum
- Haase S, Cabrera A, Langer C, Treeck M, Struck N, Herrmann S, Jansen PW, Bruchhaus I, Bachmann A, Dias S, Cowman AF, Stunnenberg HG, Spielmann T, Gilberger TW
- Infect Immun. 2008 Mar;76(3):879-87. doi: 10.1128/IAI.00144-07
2007
New therapeutic approaches for malaria: molecular aspects of erythrocyte invasion
- Spielmann T, Gilberger TW
- Dtsch Med Wochenschr. 2007 Nov;132(45):2383-6. German. doi: 10.1055/s-2007-991664
The M18 Aspartyl Aminopeptidase of the human malaria parasite Plasmodium falciparum
- Teuscher F, Lowther J, Skinner-Adams TS, Spielmann T, Dixon MW, Stack CM, Donnelly S, Mucha A, Kafarski P, Vassiliou S, Gardiner DL, Dalton JP, Trenholme KR
- J Biol Chem. 2007 Oct 19;282(42):30817-26. doi: 10.1074/jbc.M704938200
Selective permeabilization of the host cell membrane of Plasmodium falciparum-infected red blood cells with streptolysin O and equinatoxin II
- Jackson KE, Spielmann T, Hanssen E, Adisa A, Separovic F, Dixon MW, Trenholme KR, Hawthorne PL, Gardiner DL, Gilberger T, Tilley L
- Biochem J. 2007 Apr 1;403(1):167-75. doi: 10.1042/BJ20061725
2006
A cluster of ring stage-specific genes linked to a locus implicated in cytoadherence in Plasmodium falciparum codes for PEXEL negative and PEXEL positive proteins exported into the host cell
- Spielmann T, Hawthorne PL, Dixon MW, Hannemann M, Klotz K, Kemp DJ, Klonis N, Tilley L, Trenholme KR, Gardiner DL
- Mol Biol Cell. 2006 Aug;17(8):3613-24. doi: 10.1091/mbc.e06-04-0291
Organisation of ETRAMPs and EXP-1 at the parasite-host cell interface of malaria parasites
- Spielmann T, Gardiner DL, Beck HP, Trenholme KR, Kemp DJ
- Mol Microbiol. 2006 Feb;59(3):779-94. doi: 10.1111/j.1365-2958.2005.04983.x
2004
A novel Plasmodium falciparum ring stage protein, REX, is located in Maurer's clefts
- Hawthorne PL, Trenholme KR, Skinner-Adams TS, Spielmann T, Fischer K, Dixon MW, Ortega MR, Anderson KL, Kemp DJ, Gardiner DL
- Mol Biochem Parasitol. 2004 Aug;136(2):181-9. doi: 10.1016/j.molbiopara.2004.03.013
2003
MAHRP-1, a novel Plasmodium falciparum histidine-rich protein, binds ferriprotoporphyrin IX and localises to the Maurer's clefts
- Spycher C, Klonis N, Spielmann T, Kump E, Steiger S, Tilley L, Beck HP
- J Biol Chem. 2003 Sep 12;278(37):35373-83. doi: 10.1074/jbc.M305851200
etramps, a new Plasmodium falciparum gene family coding for developmentally regulated and highly charged membrane proteins located at the parasite-host cell interface
- Spielmann T, Fergusen DJ, Beck HP
- Mol Biol Cell. 2003 Apr;14(4):1529-44. doi: 10.1091/mbc.e02-04-0240
2000
Analysis of stage-specific transcription in plasmodium falciparum reveals a set of genes exclusively transcribed in ring stage parasites
- Spielmann T, Beck HP
- Mol Biochem Parasitol. 2000 Dec;111(2):453-8. doi: 10.1016/s0166-6851(00)00333-9
